scholarly journals THE INFLUENCE OF pH ON AUXIN-INDUCED ADVENTITIOUS ROOT INITIATION IN MALUS DOMESTICA

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1124b-1124
Author(s):  
James F. Harbage ◽  
Dennis P. Stimart
Keyword(s):  
Malus Domestica ◽  
Adventitious Root ◽  
Root Formation ◽  
Adventitious Root Formation ◽  
Root Number ◽  
Root Numbers ◽  
Apical Shoot ◽  
Ethanesulfonic Acid ◽  
Auxin Uptake ◽  
Influence Of Ph

Many physiological responses in plants are influenced by pH. The present chemiosmotic hypothesis suggests that auxin uptake into plant cells is governed by pH. Since auxin is used widely to enhance rooting, the influence of pH on 1H-indole-3-butyric acid (IBA) induced adventitious root formation was examined. Roots were initiated aseptically in 5 node apical shoot cuttings of micropropagated Malus domestica 'Gala'. Initiation was induced using a four day pulse in IBA and 15 g/L sucrose at pH 5.6 and 30C in the dark. Observations showed pH rose to 7.0 or greater within 1 to 2 days from microcutting placement in unbuffered initiation medium. Root numbers from shoots in media containing 1.5 μM IBA buffered with 10 mM 2[N-morpholino] ethanesulfonic acid (MES) to pH 5.5, 6.0, 6.5 or 7.0 with KOH resulted in average root numbers of 14.2, 10.9, 8.7, and 7.1, respectively, while unbuffered medium yielded 7,6 roots per shoot. Comparison of MES buffered medium at pH 5.5, 6.25 or 7.0 in factorial combination with IBA at 0, 0.15, 1.5, 15.0, and 150.0 μM resulted in a significant pH by IBA interaction for root number. At 0, 0.15 and 1.5 μM IBA root numbers were greatest at pH 5.5. At 15.0 μM IBA, pH 6.25 was optimal and at 150.0 μM IBA all three pH levels produced equivalent root numbers. A calorimetric assay to measure IBA removal from the initiation medium by microcuttings of `Gala' and `Triple Red Delicious' showed more IBA removal at pH 5.5 than at pH 7.0. Possible reasons for the effect of pH on adventitious root formation will be discussed.

scholarly journals Effects of Auxin Concentration and Medium Temperature on Four Woody Plant Taxa Propagated by Stem Cuttings using Recirculating Subirrigation in a Growth Chamber

2003 ◽  
Vol 21 (3) ◽  
pp. 126-130
Author(s):  
James S. Owen ◽  
William A. Johnson ◽  
Brian K. Maynard
Keyword(s):  
Root Formation ◽  
Woody Plant ◽  
Adventitious Root Formation ◽  
Stem Cuttings ◽  
Root Number ◽  
Medium Temperature ◽  
Root Numbers ◽  
Auxin Treatment ◽  
Auxin Concentration ◽  
Propagation Medium

Abstract Four woody plant taxa (‘Sparkleberry’ holly, ‘Mariesii’ viburnum, ‘Shasta’ viburnum, and Red Sunset® maple) were propagated from softwood stem cuttings in a recirculating subirrigation propagation system to evaluate the effect on adventitious root formation of three auxin (Dip-n-Grow®) concentrations [0 (water), 20: 1, or 10: 1] and three medium temperatures [20C (68F), 23C (74F), or 26C (79F)]. All taxa showed a positive rooting response to auxin treatment. Compared to cuttings treated with water, root number of all taxa doubled when treated with the highest auxin concentration (10: 1 Dip-n-Grow® dilution). Length of the longest root of rooted cuttings increased with auxin treatment of two taxa. Medium temperature also affected rooting of all taxa. The intermediate temperature evaluated, 23C (74F), was optimal for all rooting parameters on cuttings of ‘Mariesii’ viburnum, and for root length and percentage rooting on cuttings of ‘Shasta’ viburnum and Red Sunset® maple. A propagation medium temperature of 26C (79F) resulted in the greatest root number on cuttings of ‘Shasta’ viburnum, while 20C (68F) was optimal for root number on cuttings of Red Sunset® maple. Root numbers on cuttings of ‘Mariesii’ viburnum were reduced at 20C (68F) and 26C (79F), which appeared to be sub-and supra-optimal temperatures, respectively, for root initiation. All rooting parameters on cuttings of ‘Sparkleberry’ holly increased with propagation medium temperature. Temperature-auxin interactions were apparent for root number and length on cuttings of ‘Mariesii’ and ‘Shasta’ viburnum. The recirculating subirrigation system was useful for evaluating the basal temperature and auxin dose interactions and was effective for rooting softwood stem cuttings without intermittent mist irrigation.

scholarly journals Effect of Auxin and Wounding on Adventitious Root Formation of Prickly-pear Cactus Cladodes

1999 ◽  
Vol 9 (1) ◽  
pp. 99-102 ◽  
Author(s):  
Carlos A. Lazcano ◽  
Fred T. Davies ◽  
Andrés A. Estrada-Luna ◽  
Sharon A. Duray ◽  
Victor Olalde-Portugal
Keyword(s):  
Adventitious Root ◽  
Root Formation ◽  
Dry Mass ◽  
Adventitious Root Formation ◽  
Prickly Pear ◽  
Root Number ◽  
Commercial Practice ◽  
Prickly Pear Cactus ◽  
Cut Surface ◽  
Pear Cactus

Mature cladodes of prickly-pear cactus (Opuntia amyclaea Tenore. `Reina') were treated with five wounding methods and four concentrations of potassium salt indole-3-butyric acid (K-IBA) to stimulate adventitious root formation. The wounding method and K-IBA had highly significant effects on root number and root dry mass of cladodes. Interaction between K-IBA and wounding methods showed that greater root number was obtained at the higher auxin concentrations and with wounding methods that had the greatest cut surface area. K-IBA concentrations from 4,144 to 41,442 μm (1,000 to 10,000 mg·L-1) increased root dry mass. Only the wounding method affected rotting of cladodes. Treatments allowing suberization had a higher percentage of nonrotted cladodes. This research validates the commercial practice of allowing cladodes to suberize early in the propagation cycle. K-IBA altered rooting polarity and stimulated adventitious root formation along the wounded cladode surfaces. The vertical nonsuberized wounding methods and auxin treatments are an excellent classroom demonstration for manipulating rooting polarity. Auxin application and wounding could be of commercial benefit for enhanced rooting in the clonal regeneration of new selections for prickly-pear cactus orchards.

scholarly journals Effect of Auxin and Wounding on Adventitious Root Formation of (Opuntia amyclaea) Cladodes

HortScience ◽  
1998 ◽  
Vol 33 (4) ◽  
pp. 602d-602
Author(s):  
Carlos A. Lazcano ◽  
Fred T. Davies ◽  
Sharon A. Duray ◽  
Andres Estrada-Luna ◽  
Victor Olalde-Portugal
Keyword(s):  
Adventitious Root ◽  
Root Formation ◽  
Dry Weight ◽  
Adventitious Root Formation ◽  
Prickly Pear ◽  
Root Number ◽  
Indolebutyric Acid ◽  
Root Dry Weight ◽  
Prickly Pear Cactus ◽  
Pear Cactus

Mature cladodes of prickly-pear cactus (Opuntia amyclaea Tenore. cv. Reina) were treated with five wounding methods and four concentrations of potassium salt indolebutyric acid (K-IBA) to stimulate adventitious root formation. K-IBA from 4144 to 41,442 μm (1000 to 10,000 mg·L-1) increased root number and root dry weight; however, root length was decreased at 41,442 μm (10,000 mg·L-1). Root number and root dry weight were higher with wounding methods that had larger wounded surface areas. K-IBA altered rooting polarity and stimulated adventitious root formation along the wounded cladode surfaces. Treatments without suberization had a higher percentage of rotted cladodes. This research validates the commercial practice in Mexico of suberizing cladodes early in the propagation cycle. Auxin application could be of commercial benefit for enhanced rooting in the clonal regeneration of new selections for prickly-pear cactus orchards. The wounding methods and auxin treatments utilized make an excellent classroom demonstration for manipulating rooting polarity.

Tissue culture of Arabidopsis thaliana explants reveals a stimulatory effect of alkamides on adventitious root formation and nitric oxide accumulation

Plant Science ◽  
2008 ◽  
Vol 174 (2) ◽  
pp. 165-173 ◽  
Author(s):  
Juan Carlos Campos-Cuevas ◽  
Ramón Pelagio-Flores ◽  
Javier Raya-González ◽  
Alfonso Méndez-Bravo ◽  
Randy Ortiz-Castro ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Tissue Culture ◽  
Arabidopsis Thaliana ◽  
Adventitious Root ◽  
Root Formation ◽  
Adventitious Root Formation
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