scholarly journals 876 PB 238 MICROPROPAGATION OF UNIOLA PANICULATA L. (SEA OATS) FROM TILLER EXPLANTS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 559d-559 ◽  
Author(s):  
Nancy Phiman ◽  
Michael E. Kane
Keyword(s):  
Shoot Organogenesis ◽  
Shoot Multiplication ◽  
Shoot Tips ◽  
Mineral Salts ◽  
Lateral Shoot ◽  
Uniola Paniculata ◽  
Leaf Surfaces ◽  
Sea Oats ◽  
Accepted Practice ◽  
Control Erosion

Beach stabilization by replanting dune species such as Uniola paniculata L. (Sea Oats), is an accepted practice to control erosion in the southeastern United States. Increased restrictions on collection of sea oat seed and plant material for propagation is of increasing concern. Development of micropropagation protocols for establishment and production of sea oats from donor plants of known phenotype would be useful for selecting and producing plants with commercially valuable characteristics. Terminal and lateral shoot tips (3 mm wide and 4 mm high) from containerized plants were surface sterilized and established on Linsmaier & Skoog mineral salts and organics supplemented with 87.6 mM sucrose, 2.2 μM benzyladenine solidified with 0.8% TC® Agar. Terminal tiller shoot tips were more responsive than lateral shoot tips. Four monthly subcultures were. required for stabilized shoot multiplication from culture lines established from terminal tiller shoot tips. Shoot organogenesis frequently occurred from the cut leaf surfaces of subcultured shoot clusters. Microcuttings were established ex vitro in plug cells containing sand or vermiculite.

scholarly journals Sea Oats, Uniola paniculata

EDIS ◽  
2018 ◽  
Vol 2018 (5) ◽  
Author(s):  
Debbie Miller ◽  
Mack Thetford ◽  
Chris Verlinde ◽  
Gabriel Campbell ◽  
Ashlynn Smith
Keyword(s):  
Wind Waves ◽  
High Tide ◽  
Coastal Areas ◽  
Salt Tolerant ◽  
Uniola Paniculata ◽  
Vertical Growth ◽  
Sea Oats ◽  
In The Wild ◽  
Florida Panhandle ◽  
High Tide Line

Sea oats occur throughout Florida on beach dunes and beaches and on coastal areas west to Texas and north to Maryland. Sea oats are vital dune builders that accumulate sand and prevent erosion due to wind, waves, and large storms. As sand is trapped by the long leaves of sea oats, vertical growth is stimulated, and rooting occurs at the buried nodes. This plant is extremely drought- and salt-tolerant, grows up to the high tide line of beaches, and propagates both vegetatively and by seed in the wild (Shadow 2007).https://edis.ifas.ufl.edu/sg186 This publication is derived from information in SGEB-75/SG156, Dune Restoration and Enhancement for the Florida Panhandle, by Debbie Miller, Mack Thetford, Christina Verlinde, Gabriel Campbell, and Ashlynn Smith. https://edis.ifas.ufl.edu/sg156.

scholarly journals 588 PB 129 IN VITRO MULTIPLICATION OF STEWARTIA MALACODENDRON L., AN ENDANGERED WOODY SPECIES

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 516b-516
Author(s):  
Murdock Ray Gillis ◽  
Michael E. Kane
Keyword(s):  
Woody Plant ◽  
Unit Cost ◽  
Woody Species ◽  
Shoot Multiplication ◽  
Shoot Tips ◽  
Nodal Segments ◽  
Deciduous Tree ◽  
Landscape Plant ◽  
Woody Plant Medium

Stewartia malacodendron L. (silky camellia), a small deciduous tree bearing showy flowers, has potential as a landscape plant. Propagation problems, limited availability and consequent high unit cost have slowed its acceptance as a landscape plant. Given its potential value as a landscape plant, studies were initiated to define a micropropagation protocol. Surface sterilized shoot tips and nodal explants from two-year-old container grown seedlings were established on Woody Plant Medium supplemented with 4.44 μM benzyladenine (BA) and solidified with 0.8% TC® Agar. Sustained growth of subcultured shoot tips and nodal segments required the addition of 8-15 μM gibberellic acid (GA3) to the medium. Regenerated shoots were 3 - 5 cm long, unbranched and typically consisted of three subdivisions. Effects of cytokinin type (BA, 2iP or kinetin) and concentration (0-25 μM) with factorial combinations of GA3 (0-30 μM) on shoot multiplication, elongation and diameter were determined after a 28 day culture period. Moderate GA, levels (10 & 20 μM) in combination with 2.5 μM BA yielded the highest quality microcuttings.

scholarly journals Cryopreservation of cherry rootstock Gisela 5 (Prunus cerasus × Prunus canescens) shoot tips by droplet-vitrification technique

2013 ◽  
Vol 21 (2) ◽  
pp. 79-85 ◽  
Author(s):  
Djurdjina Ružić ◽  
Tatjana Vujović ◽  
Radosav Cerović
Keyword(s):  
Liquid Medium ◽  
Room Temperature ◽  
Sucrose Concentration ◽  
Shoot Multiplication ◽  
Shoot Tips ◽  
Prunus Cerasus ◽  
Droplet Vitrification ◽  
Vitrification Solution ◽  
Gisela 5

ABSTRACT The droplet-vitrification technique was applied to in vitro shoot tips of cherry rootstock Gisela 5 (Prunus cerasus × Prunus canescens). Explants were precultured in the dark at 23 °C, in liquid MS medium with a progressively increasing sucrose concentration (0.3 M for 15 h, then 0.7 M for 5 h). Loading involved a 30 min incubation of explants in a solution comprising 1.9 M glycerol and 0.5 M sucrose. Explants were dehydrated at room temperature using a solution PVS A3 [Murashige and Skoog (MS) liquid medium, 22.5% (w/v) sucrose, 37.5% (w/v) glycerol, 15% (w/v) ethylene glycol and 15% (w/v) dimethylsulfoxide] for 30, 40 and 50 min and the PVS3 solution [MS liquid medium, 50% (w/v) sucrose, 50% (w/v) glycerol] for 60, 90 and 120 min. Explants were cooled by direct immersion in liquid nitrogen (LN) in 10 μl droplets of vitrification solution placed on aluminum foil strips. The foil strips were retrieved from LN and immersed in preheated (37 °C) unloading solution (0.8 M sucrose) for 30 s, and an equal volume of unloading solution at room temperature was added for further incubation for 30 min. Shoot tips were transferred onto the regrowth medium, cultivated in the dark for 7 days before being incubated under standard conditions. Three weeks after transferring the shoot tips onto the regrowth medium, the survival rate of control and cryopreserved explants of Gisela 5 dehydrated with PVS A3 was 100%, regardless of the treatment duration. After dehydration with solution PVS3, the survival varied between 70 and 100% for control explants and 78 and 95% for cryopreserved shoot tips. Gisela 5 shoot tips dehydrated for 40 min with PVS A3 vitrification solution demonstrated the best regrowth (38%). When using the PVS3 solution, survival of cryopreserved shoot tips was the highest (95%) after 60 min treatment followed by 40% regrowth. After three successive subcultures on shoot multiplication, medium shoots recovered viability, multiplication ability and morphology equal of that prior to cryopreservation.

In vitro propagation of Camellia fascicularis: a plant species with extremely small populations

2020 ◽  
Vol 100 (2) ◽  
pp. 202-208
Author(s):  
Mengting Wang ◽  
Guiliang Zhang ◽  
Peiyao Xin ◽  
Yun Liu ◽  
Bin Li ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Large Scale ◽  
In Vitro Regeneration ◽  
Germplasm Conservation ◽  
Shoot Multiplication ◽  
Shoot Tips ◽  
Naphthalene Acetic Acid ◽  
Multiplication Rate ◽  
Ms Medium

Camellia fascicularis is an endangered evergreen ornamental plant with pale yellow flowers. An efficient and reproducible in vitro regeneration method is required for its large-scale propagation and germplasm conservation. In this study, one axillary bud per nodal stem was obtained from C. fascicularis cultured on Murashige & Skoog (MS) medium containing 0.1 mg L−1 indole-3-acetic acid (IAA) combined with 1.0 mg L−1 6-benzylaminopurine (BA). Axillary buds from the stem segments were transferred to modified woody plant medium (WPM) supplemented with 3.0 mg L−1 BA in combination with 0.3 mg L−1 IAA for multiplication, thereby resulting in a high shoot multiplication rate of 6.8. Multiple shoots were divided into nodal stems and shoot tips and were induced to root. The shoot tips were induced to root by culturing on one-half MS medium supplemented with 2.0 mg L−1 indole-3-butyric acid (IBA) in combination with 0.3 mg L−1 α-naphthalene acetic acid (NAA), which resulted in 76.0% rooting efficiency with 2.3 roots per shoot. The optimal hormone ratio for inducing rooting of nodal stems was 1.0 mg L−1 IBA in combination with 2.0 mg L−1 NAA, which resulted in 72.7% rooting efficiency with 1.7 roots per nodal stem. These two rooted plantlets were successfully acclimatized and established in a greenhouse.

scholarly journals Micropropagation of Rubus spp.

1986 ◽  
Vol 58 (4) ◽  
pp. 193-196
Author(s):  
Åke Finne
Keyword(s):  
Phenolic Compounds ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Shoot Tips

Rapid micropropagation of ‘Black Satin’, ‘Thornless Evergreen’ and ‘Bedford Giant’ was achieved by culturing shoot tips in a 1/1 MS. The best medium in the introduction stage contained 2.0 mg/l BAP. Shoot proliferation occurred with 3.0 mg/1BAP. Some problems with callus and phenolic compounds appeared in both introduction and shoot multiplication stages. The plantlets were rooted either in 1/10 MS without hormones or directly in peat. Two months later most of ‘Black Satin’ and ‘Bedford Giant’ and about half of ‘Thornless Evergreen’ were growing steadily. Theoretically it is possible to produce 60,000 plants within a half year by this method.

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