Specific Genomic Regions in Common Bean Condition Resistance to Multiple Pathogens

A genetic linkage map of 170 RAPD markers mapped across 79 recombinant inbred lines (Dorado and XAN-176) reveal genomic regions that condition multiple disease resistance to fungal (Ashy Stem Blight—Macrophomina phaseolina), viral (bean golden mosaic virus—BGMV), and bacterial (common bacterial blight—Xanthomonas campestris pv. phaseoli) pathogens of common bean (Phaseolus vulgaris). A genomic site on linkage group US-1 had a major effect, explaining 18%, 34%, and 40% of the variation in phenotypic reaction to ashy stem blight, BGMV, and common bacterial blight disease, respectively. Adjacent to this region was a QTL conditioning 23% of the variation in reaction to another fungal pathogen, web blight (Thanatephorus cucumeris). A second genomic site on linkage group US-1 had minor affect on multiple resistance expression to the same fungal (15%), viral (15%), and bacterial (10%) pathogens. It is unknown whether these specific genomic regions represent a series of linked QTL affecting resistance to each disease separately or an individual locus with pleiotropic effect against all three pathogens.

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Molecular Markers Associated with Plant Architecture and Resistance to Common Blight, Web Blight, and Rust in Common Beans

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.121.5.794 ◽

1996 ◽

Vol 121 (5) ◽

pp. 794-803 ◽

Cited By ~ 56

Author(s):

Geunhwa Jung ◽

Dermot P. Coyne ◽

Paul W. Skroch ◽

James Nienhuis ◽

E. Arnaud-Santana ◽

...

Keyword(s):

Common Bean ◽

Linkage Map ◽

Recombinant Inbred ◽

Bacterial Blight ◽

Xanthomonas Campestris ◽

Random Amplified Polymorphic Dna ◽

Recombinant Inbred Lines ◽

Rapd Marker ◽

Common Bacterial Blight ◽

Web Blight

Random amplified polymorphic DNA (RAPD) markers were used to construct a partial linkage map in a recombinant inbred population derived from the common bean (Phaseolus vulgaris L.) cross BAC 6 × HT 7719 for studying the genetics of disease resistance in common bean. The linkage map spanned 545 cM and included 75 of 84 markers used in this study. The population of 128 recombinant inbred lines was evaluated for resistance to common bacterial blight, foliar resistance to web blight [WB; Thanatephorus cucumeris (Frank) Donk], and resistance to rust [Uromyces appendiculatus var. appendiculatus (Pers.:Pers) Unger]. Common bacterial blight [CBB; Xanthomonas campestris pv. phaseoli (Smith) Dye] resistance was evaluated for CBB strain Epif-IV in later-developed trifoliolate leaves and for CBB strain EK-11 in seeds, first trifoliolate leaves, later-developed trifoliolate leaves, and pods. In addition, lines were rated for plant uprightness and branch density. Two to six markers accounted for 14% to 34% of the phenotypic variation for each trait. Significant marker locustrait associations were found for 14 mapped loci and 7 of the 9 unmapped markers. The distribution of detected QTL appeared to be nonrandom with most significant markers associated with more than one trait or closely linked to markers significantly associated with variation for a different trait. One marker, BC4091250, was significantly associated with WB resistance, resistance for CBB strain Epif-IV in later-developed trifoliolate leaves, and resistance for CBB strain EK-11 in first trifoliolate leaves, later-developed trifoliolate leaves, and pods. A rust resistance gene was mapped in an interval 14.6 cM from RAPD marker H191050 and 12.5 cM from marker AJ16250.

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Molecular-marker-based Genetic Analysis of Tepary Bean-derived Common Bacterial Blight Resistance in Different Developmental Stages of Common Bean

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.122.3.329 ◽

1997 ◽

Vol 122 (3) ◽

pp. 329-337 ◽

Cited By ~ 43

Author(s):

Geunhwa Jung ◽

Paul W. Skroch ◽

Dermot P. Coyne ◽

James Nienhuis ◽

E. Arnaud-Santana ◽

...

Keyword(s):

Linkage Group ◽

Common Bean ◽

Linkage Map ◽

Recombinant Inbred ◽

Bacterial Blight ◽

Chromosomal Region ◽

Bacterial Disease ◽

Common Bacterial Blight ◽

Group 5 ◽

Group 1

Randomly amplified polymorphic DNA (RAPD) molecular markers were used to construct a partial genetic linkage map in a recombinant inbred population derived from the common bean (Phaseolus vulgaris L.) cross PC-50 × XAN-159 for studying the genetics of bacterial disease resistance in common bean. The linkage map spanned 426 cM and included 168 RAPD markers and 2 classical markers with 11 unassigned markers. The seventy recombinant inbred lines were evaluated for resistance to two strains of common bacterial blight [Xanthomonas campestris pv. phaseoli (Smith) Dye] (Xcp). Common bacterial blight (CBB) resistance was evaluated for Xcp strain EK-11 in later-developed trifoliolate leaves and for Xcp strains, DR-7 and EK-11, in first trifoliolate leaves, seeds, and pods. One to four quantitative trait loci (QTLs) accounted for 18% to 53% of the phenotypic variation for traits. Most significant effects for CBB resistance were associated with one chromosomal region on linkage group 5 and with two regions on linkage group 1, of the partial linkage map. The chromosomal region (a 13-cM interval) in linkage group 5 was significantly associated with resistance to Xcp strains DR-7 and EK-11 in leaves, pods, and seeds. The regions in linkage group 1 were also significantly associated with resistance to both Xcp strains in more than one plant organ. In addition, a seedcoat pattern gene (C) and a flower color gene (vlae) were mapped in linkage groups 1 and 5, respectively, of the partial linkage map. The V locus was found to be linked to a QTL with a major effect on CBB resistance.

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Gene Estimation, Associations of Traits, and Confirmation of QTL for Common Bacterial Blight Resistance in Common Bean

HortScience ◽

10.21273/hortsci.33.3.499e ◽

1998 ◽

Vol 33 (3) ◽

pp. 499e-499

Author(s):

Soon O. Park ◽

Dermot P. Coyne ◽

Geunhwa Jung

Keyword(s):

Common Bean ◽

Phenotypic Variation ◽

Bacterial Blight ◽

Xanthomonas Campestris ◽

Major Gene ◽

Flower Color ◽

Common Bacterial Blight ◽

Total Phenotypic Variation ◽

Recombinant Inbred Population ◽

Serious Disease

Common bacterial blight, incited by Xanthomonas campestris pv. phaseoli (Xcp), is a serious disease of common bean(Phaseolus vulgaris L.). Gene estimation, associations of traits, and confirmation of QTL for resistance to Xcp were investigated in a recombinant inbred population derived from the backcross BC2F6 PC-50 (susceptible to Xcp) × XAN-159 (resistant to Xcp). One or two genes from XAN-159 controlled leaf resistance to Xcp. One major gene from XAN-159 was involved in controlling pod resistance to Xcp. Low (+0.24) to intermediate (+0.57 and +0.75) Pearson correlations were observed between leaf and pod reactions to Xcp. Purple flower color was associated with leaf and pod resistance to Xcp but not days to flower. One to 2 QTLs explained from 20 to 51% of the total phenotypic variation for leaf reactions to 5 Xcp strains. Two QTLs explained from 20 to 22% of the total phenotypic variation for pod reactions to Xcp strains EK-11 and DR-7. A marker BC437.1050 was associated with leaf and pod resistance to 5 Xcp strains in nearly all experiments, and accounted for 13% to 45% of the phenotypic variation for these traits. A unassigned marker D13.1000 was associated with only pod resistance to Xcp strains EK-11 and DR-7. Gene number (1 or 2) estimations and number of QTL (1 or 2) detected for resistance to Xcp generally agree. The confirmed marker BC437.1050 is expected to be useful in breeding programs for resistance to Xcp.

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Selecting for Common Bacterial Blight Resistance in Common Bean: Effects of Multiple Leaf Inoculation and Detached Pod Inoculation Test

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.123.5.864 ◽

1998 ◽

Vol 123 (5) ◽

pp. 864-867 ◽

Cited By ~ 4

Author(s):

H.M. Ariyarathne ◽

D.P. Coyne ◽

A.K. Vidaver ◽

K.M. Eskridge

Keyword(s):

Common Bean ◽

Bacterial Blight ◽

Xanthomonas Campestris ◽

Heritability Estimate ◽

Accurate Estimate ◽

Common Bacterial Blight ◽

Plant Organs ◽

Disease Reaction ◽

Split Plot ◽

Similar Disease

Breeding for resistance is an important strategy to manage common bacterial blight disease caused by Xanthomonas campestris pv. phaseoli (E. Smith) Dye (Xep) in common bean (Phaseolus vulgaris L.). It is necessary to determine if prior inoculation of the first trifoliolate leaf with Xcp influences subsequent reactions in other plant organs by increasing or decreasing resistance to Xcp. It is difficult to get an accurate estimate of heritability of disease reaction in pods since environment greatly affects the heritability estimate if flowering occurs over extended time periods. Thus, the disease reaction in attached pods versus detached pods was compared. A split-split plot design with two replications (growth chambers as blocks) was used, with bean lines as the whole-plot factors, Xcp strains as subplot factors, and bacterial inoculation treatments for leaf reactions or pod treatments as split-split plot factors. The first trifoliolate leaves, later developed leaves, and attached and detached pods were inoculated. No effects of prior inoculation on the disease reactions of subsequently inoculated leaves and pods were observed, indicating that the different plant organs can be inoculated at different times. The fact that detached and attached pods showed similar disease symptoms would suggest use of the former to reduce environment variance and improve heritability estimates of resistance.

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Bacterial, Fungal, and Viral Disease Resistance Loci Mapped in a Recombinant Inbred Common Bean Population (`Dorado'/XAN 176)

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.125.4.476 ◽

2000 ◽

Vol 125 (4) ◽

pp. 476-481 ◽

Cited By ~ 62

Author(s):

Phillip N. Miklas ◽

Richard Delorme ◽

Valerie Stone ◽

Mark J. Daly ◽

J. Rennie Stavely ◽

...

Keyword(s):

Disease Resistance ◽

Quantitative Trait Loci ◽

Common Bean ◽

Quantitative Trait ◽

Recombinant Inbred ◽

Bacterial Blight ◽

Scar Markers ◽

Common Bacterial Blight ◽

Linkage Groups ◽

Stem Blight

Understanding the genomic associations among disease resistance loci will facilitate breeding of multiple disease resistant cultivars. We constructed a genetic linkage map in common bean (Phaseolus vulgaris L.) containing six genes and nine quantitative trait loci (QTL) comprising resistance to one bacterial, three fungal, and two viral pathogens of bean. The mapping population consisted of 79 F5:7 recombinant inbred lines (RILs) derived from a `Dorado'/XAN 176 hybridization. There were 147 randomly amplified polymorphic DNA (RAPD) markers, two sequence characterized amplified region (SCAR) markers, one intersimple sequence repeat (ISSR) marker, two seedcoat color genes R and V, the Asp gene conditioning seed brilliance, and two rust [Uromyces appendiculatus var. appendiculatus (Pers.:Pers) Unger] resistance genes: one conditioning resistance to Races 53 and 54 and the other conditioning resistance to Race 108. These markers mapped across eleven linkage groups, one linked triad, and seven linked pairs for an overall map length of 930 cM (Kosambi). Genes conditioning resistance to anthracnose (Co-2) [Colletotrichum lindemuthianum (Sacc. and Magnus) Lams.-Scrib.], bean rust (Ur-5), and bean common mosaic virus (I and bc-3) (BCMV) did not segregate in this population, but were mapped by inference using linked RAPD and SCAR markers identified in other populations. Nine previously reported quantitative trait loci (QTL) conditioning resistance to a variety of pathogens including common bacterial blight [Xanthomonas campestris pv. phaseoli (Smith) Dye], ashy stem blight [Macrophomina phaseolina (Tassi) Goid.], and bean golden mosaic virus (BGMV), were located across four linkage groups. Linkage among QTL for resistance to ashy stem blight, BGMV, and common bacterial blight on linkage group B7 and ashy stem blight, BGMV, and rust resistance loci on B4 will complicate breeding for combined resistance to all four pathogens in this population.

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075 Confirmation of Molecular Markers and Flower Color Associated with QTL for Resistance to Common Bacterial Blight in Common Beans

HortScience ◽

10.21273/hortsci.34.3.454b ◽

1999 ◽

Vol 34 (3) ◽

pp. 454B-454

Author(s):

Soon O. Park ◽

Dermot P. Coyne ◽

Nedim Mutlu ◽

James R. Steadman ◽

Geunhwa Jung

Keyword(s):

Linkage Group ◽

Bacterial Blight ◽

Xanthomonas Campestris ◽

Rapd Markers ◽

Rapd Marker ◽

Flower Color ◽

Common Bacterial Blight ◽

Backcross Population ◽

Leaf Resistance ◽

Group 5

Common bacterial blight, incited by Xanthomonas campestris pv. phaseoli (Xcp), is a serious disease of common bean (Phaseolus vulgaris). RAPD markers and flower color (V gene) previously had been reported to be associated with six QTL affecting leaf and pod resistance to Xcp. However, the markers for the QTL were not confirmed in different populations and environments to indicate their merit in breeding. Our objective was to determine if the associations of RAPD markers and the V gene with QTL for leaf and pod resistance to Xcp in a RI backcross population from the cross BC2F6 `PC-50' × XAN-159 and for leaf resistance to Xcp in a F2 population from a different cross Pinto `Chase' × XAN-159 could be confirmed. Among six QTL previously detected, five in the RI backcross population and three in the F2 population were confirmed to be associated with resistance to Xcp. The V gene and RAPD marker BC437.1050 on linkage group 5 were most consistently associated with leaf and pod resistance to two to five XCP strains in the RI backcross population and with leaf resistance to two Xcp strains in the F2 population. The confirmed marker BC437.1050 and V gene on linkage group 5, along with other resistance genes from other germplasm, could be used to pyramid the different genes into a bean cultivar to enhance the resistance to Xcp.

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The genetic background of resistance to common bacterial blight in newly identified common bean lines on the basis of inheritance studies

International Journal of Horticultural Science ◽

10.31421/ijhs/6/1/73 ◽

2000 ◽

Vol 6 (1) ◽

Author(s):

E. Mergenthaler ◽

Bisztray Gy.

Keyword(s):

Common Bean ◽

Genetic Background ◽

Bacterial Blight ◽

Xanthomonas Campestris ◽

Resistance Breeding ◽

Inheritance Of Resistance ◽

Common Bacterial Blight ◽

Phaseolus Vulgaris L ◽

Coupling Phase ◽

Recessive Genes

Common bacterial blight (CBB), caused by Xanthomonas campestris pv. phaseoli (Xcp). is a major disease problem of common bean (Phaseolus vulgaris L.). The inheritance of resistance in Xrl and Xr2 lines to two isolates of Xcp was studied in the F2 and F3 populations from the crosses between these lines and the Masay variety (susceptible to Xcp). Segregation patterns indicated that different single recessive genes presumably in coupling phase linkage determined the resistance to the HUN and EK-1 1 strains of Xcp in both lines. The presence of some minor, modifying genes beside the monogenic genetic background of resistance was also observed. Xrl and Xr2 lines represent valuable new monogenic genetic sources in resistance breeding to CBB.

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Confirmation of QTL Associated with Common Bacterial Blight Resistance in Phaseolus vulgaris L.

HortScience ◽

10.21273/hortsci.31.4.623f ◽

1996 ◽

Vol 31 (4) ◽

pp. 623f-623

Author(s):

Geunhwa Jung ◽

Paul W. Skroch ◽

Dermot P. Coyne ◽

James Nienhuis ◽

E. Arnaud-Santana

Keyword(s):

Common Bean ◽

Recombinant Inbred ◽

Bacterial Blight ◽

Xanthomonas Campestris ◽

Rapd Marker ◽

Marker Locus ◽

Common Bacterial Blight ◽

Backcross Population ◽

Transfer Resistance ◽

Inbred Backcross

Common bacterial blight (CBB) incited by the bacterial pathogen Xanthomonas campestris pv. phaseoli (Smith) Dye is an important disease of common bean. In a previous study, QTL associated with CBB resistance were described based on RAPD marker analysis of a recombinant inbred population derived from the common bean cross BAC-6 (R) × HT-7719 (S) (resistant × susceptible). The objective of this research is to confirm these previously described candidate marker locus-QTL associations using an inbred backcross PC-50 (S) × BAC-6 (R) and a recombinant inbred Venezuela 44 (S) × BAC-6 (R) population. Two markers previously found to be associated with QTL for CBB resistance in the BAC-6 × HT-7719 population were found to account for 30% of the phenotypic variation for CBB resistance in the PC-50 × BAC-6 inbred backcross population. The three most resistant BC2F3 lines based on marker locus genotypes were ranked 1, 3, and 7 (out of 64) based on phenotypic evaluation. These results provide important confirmation of marker locus-QTL associations and indicate that RAPD markers linked to loci controlling the expression of CBB resistance in common bean may be used to transfer resistance genes into susceptible breeding material.

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Sources of Resistance to Common Bacterial Blight and Rust in Elite Phaseolus vulgaris L. Germplasm

HortScience ◽

10.21273/hortsci.28.6.644 ◽

1993 ◽

Vol 28 (6) ◽

pp. 644-646 ◽

Cited By ~ 8

Author(s):

E. Arnaud-Santana ◽

M.T. Mmbaga ◽

D.P. Coyne ◽

J.R. Steadman

Keyword(s):

Phaseolus Vulgaris ◽

Common Bean ◽

Bacterial Blight ◽

Xanthomonas Campestris ◽

Uromyces Appendiculatus ◽

Common Bacterial Blight ◽

Phaseolus Vulgaris L ◽

Sources Of Resistance ◽

The Common

We studied leaf and pod reactions of 18 Phaseolus vulgaris germplasm lines (three temperate and 15 tropical) to four Xanthomonas campestris pv. phaseoli (XCP) (Smith) Dye strains and seven Uromyces appendiculatus (UA) (Pers.) Unger races. Line × XCP interaction was significant for leaf and pod reactions. The common bean lines XAN-159, BAC-6, and XAN-112 had the best combined leaf and pod resistance to XCP. Line × UA race interactions were significant (P = 0.05). Lines IAPAR-14 and BAC-6 had the best combined resistance to XCP and UA.

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Toward an integrated linkage map of common bean. III. Mapping genetic factors controlling host-bacteria interactions.

Genetics ◽

10.1093/genetics/134.1.341 ◽

1993 ◽

Vol 134 (1) ◽

pp. 341-350 ◽

Cited By ~ 1

Author(s):

R O Nodari ◽

S M Tsai ◽

P Guzmán ◽

R L Gilbertson ◽

P Gepts

Keyword(s):

Linkage Group ◽

Common Bean ◽

Linkage Map ◽

Genetic Control ◽

Xanthomonas Campestris ◽

Chromosome Region ◽

Rhizobium Tropici ◽

Plant Host ◽

Putative Qtls ◽

Genomic Regions

Abstract Restriction fragment length polymorphism (RFLP)-based genetic linkage maps allow us to dissect the genetic control of quantitative traits (QT) by locating individual quantitative trait loci (QTLs) on the linkage map and determining their type of gene action and the magnitude of their contribution to the phenotype of the QT. We have performed such an analysis for two traits in common bean, involving interactions between the plant host and bacteria, namely Rhizobium nodule number (NN) and resistance to common bacterial blight (CBB) caused by Xanthomonas campestris pv. phaseoli. Analyses were conducted in the progeny of a cross between BAT93 (fewer nodules; moderately resistant to CBB) and Jalo EEP558 (more nodules; susceptible to CBB). An RFLP-based linkage map for common bean based on 152 markers had previously been derived in the F2 of this cross. Seventy F2-derived F3 families were inoculated in separate greenhouse experiments with Rhizobium tropici strain UMR1899 or X. c. pv. phaseoli isolate isolate W18. Regression and interval mapping analyses were used to identify genomic regions involved in the genetic control of these traits. These two methods identified the same genomic regions for each trait, with a few exceptions. For each trait, at least four putative QTLs were identified, which accounted for approximately 50% and 75% of the phenotypic variation in NN and CBB resistance, respectively. A chromosome region on linkage group D7 carried factor(s) influencing both traits. In all other cases, the putative QTLs affecting NN and CBB were located in different linkage groups or in the same linkage group, but far apart (more than 50 cM). Both BAT93 and Jalo EEP558 contributed alleles associated with higher NN, whereas CBB resistance was always associated with BAT93 alleles. Further investigations are needed to determine whether the QTLs for NN and CBB on linkage group D7 represent linked genes or the same gene with pleiotropic effects. Identification of the QTLs raises the possibility of initiating map-based cloning and marker-assisted selection for these traits.

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