Inheritance of Flower Color in Anagallis monelli L.

Plants of Anagallis monelli in their native habitat or in cultivation have either blue or orange flowers. Clonally propagated cultivars, seed obtained from commercial sources and the resulting plants were grown in a greenhouse at the University of New Hampshire. F2 progeny obtained from hybridization between blue- and orange-flowered plants had blue, orange or red flowers. There were no significant differences in petal pH of orange-, blue-, and red-flowered plants that could explain the differences in flower color. Anthocyanidins were characterized by high-performance liquid chromatography. Results indicated that blue color was due to malvidin, orange to pelargonidin, and red to delphinidin. Based on our segregation data, we propose a three-gene model to explain flower color inheritance in this species.

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Update on the Ornamental Breeding Program at the University of New Hampshire

HortScience ◽

10.21273/hortsci.40.4.1100e ◽

2005 ◽

Vol 40 (4) ◽

pp. 1100E-1101 ◽

Cited By ~ 1

Author(s):

Rosanna Freyre

Keyword(s):

New Hampshire ◽

Interspecific Hybrids ◽

Floral Development ◽

Flower Color ◽

Breeding Program ◽

Breeding Lines ◽

Desert Areas ◽

The University ◽

University Of New Hampshire ◽

Blue Eyes

The Ornamental Breeding Program at the University of New Hampshire (UNH) was initiated in 1998, aiming to develop new or improved vegetatively propagated cultivars. Initially, breeding focused on Anagallis monelli (Pimpernel). At the time, only one blue and one orange cultivar (`Skylover Blue' and `Sunrise') were grown commercially. Main breeding goals were to develop plants with more compact habit and earlier flowering in the spring. In 2002, the first two UNH cultivars were released as Proven Selections™: Anagallis`Wildcat Blue' and `Wildcat Orange'. We have also developed breeding lines with new pink, violet, lilac, and white flower colors that are currently in industry trials. Studies on genetics, biochemistry, and anatomy of flower color in A. monelli have been performed and molecular studies are in progress. Breeding of Nolana and Browallia started in 2000 and UNH lines are currently in industry trials. Nolana is comprised of over 80 species native to desert areas of Peru and Chile. Only two cultivars, N. paradoxa`Bluebird' and `Snowbird', and interspecific hybrid `Blue Eyes' are currently commercially available. We now have several Nolana species at UNH representing a wide germplasm base. Based on ornamental potential, some species have been selected for breeding, aiming to develop sterile interspecific hybrids. Studies to break seed dormancy to optimize germination rates are in progress, as well as research on floral development, which is being conducted in collaboration with Peruvian researchers. Interspecific hybridizations have been used in Browallia to develop breeding lines with new or improved traits than those available from seed cultivars.

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Genetics and Anthocyanin Analysis of Flower Color in Mexican Petunia

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.140.1.45 ◽

2015 ◽

Vol 140 (1) ◽

pp. 45-49 ◽

Cited By ~ 6

Author(s):

Rosanna Freyre ◽

Chad Uzdevenes ◽

Liwei Gu ◽

Kenneth H. Quesenberry

Keyword(s):

Mass Spectrometry ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

High Performance ◽

Flower Color ◽

Liquid Chromatography Mass Spectrometry ◽

Color Combination ◽

Pink Color ◽

Color Purple ◽

Corolla Color

The genetics and anthocyanins responsible for flower color were studied in Ruellia simplex Wright (mexican petunia). An F2 population with 153 individuals segregating for four flower colors was developed from a cross between a maternal individual with white corolla with purple throat (WP) and a paternal individual with pink corolla (PK). All the F1 generation had fully purple flowers (P). The F2 generation segregated 94 P:30 PK:24 WP:5 WPK (WPK is a new color combination of white corolla limb and pink throat). These data were separated into groups for corolla limb color and for throat color. The ratio for corolla limb color segregated 94 P:30 PK:29 W, which fits a 9:3:4 recessive epistasis interaction (P = 0.22). The data for corolla throat segregated 118 P:35 PK, which fits a 3:1 ratio (P = 0.54). High-performance liquid chromatography mass spectrometry analyses were performed to elucidate the anthocyanins responsible for the four obtained flower colors. We found that delphinidin derivatives conferred purple corolla color, whereas pelargonidin derivatives were responsible for the pink corolla color. Purple corolla throat color was the result of delphinidin derivatives, whereas the pink color was the result of peonidin derivatives.

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Cross-validation of a high-performance liquid chromatography nevirapine plasma assay in a resource-limited setting in Zimbabwe

African Journal of Laboratory Medicine ◽

10.4102/ajlm.v10i1.1264 ◽

2021 ◽

Vol 10 (1) ◽

Author(s):

Faithful Makita-Chingombe ◽

Anthony T. Podany ◽

Timothy Mykris ◽

Farai Muzambi ◽

Richard W. Browne ◽

...

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

High Performance ◽

Cross Validation ◽

Medical Center ◽

Resource Limited ◽

Aids Clinical Trials ◽

The University ◽

University Of Zimbabwe ◽

Plasma Assay

An international HIV pharmacology specialty laboratory (PSL) was established at the University of Zimbabwe to increase bioanalytical and investigator capacities. Quantitation of plasma nevirapine in samples from the AIDS Clinical Trials Group protocol 5279 was compared between the University of Nebraska Medical Center PSL and the University of Zimbabwe PSL. Both PSLs employed internally developed methods utilising reverse-phase high-performance liquid chromatography with ultraviolet detection. Eighty-seven percent of the cross-validation results exhibited ± 20% difference.

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