scholarly journals Intergeneric Hybrids between Weigela and Diervilla (Caprifoliaceae)

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 1008D-1008 ◽  
Author(s):  
Darren Touchell ◽  
Zenaida Viloria ◽  
Thomas Ranney
Keyword(s):  
Tissue Culture ◽  
North America ◽  
Cold Hardiness ◽  
Northeast Asia ◽  
Intergeneric Hybrids ◽  
Related Genus ◽  
Ex Vitro ◽  
Embryo Abortion ◽  
Intergeneric Crosses ◽  
Cleaved Amplified Polymorphic Sequences

Weigela Thunb. consists of 12 species distributed throughout Northeast Asia. Diervilla Mill. is a closely related genus containing three species endemic to North America. Taxa from both of these genera are important nursery crops. Hybrids between these genera could potentially combine the excellent cold hardiness and adaptability of Diervilla with diverse forms, foliage colors, and flower colors found in Weigela. Prior attempts to create intergeneric hybrids between these genera were unsuccessful and resulted in embryo abortion before seeds matured. To overcome this barrier, ovule culture and micropropagation procedures were used to develop intergeneric hybrids. Cleaved amplified polymorphic sequences (CAPS) analysis was used to verify hybrids. Intergeneric crosses, D. lonicera × W. middendorfiana, D. sessilifolia × W. florida (two clones), and D. lonicera × W. florida were attempted. Crosses of D. lonicera × W. middendorfiana did not produce viable hybrids. From the remaining three crosses, a total of 544 plants were obtained from 1278 ovules. About 85% of the 544 plants appeared very chlorotic or had low vigor, and senesced when transferred to multiplication medium. Only 80 of the 544 plants were successfully maintained in tissue culture, of which 10 have been successfully transferred ex vitro. CAPS analysis indicated that a majority of these plants were hybrids. Further studies are focused on improving tissue culture procedures and other methods to develop tetraploids to increase plantlet vigor and fertility.

scholarly journals Interploid Hybridizations in Ornamental Cherries Using Prunus maackii

2012 ◽  
Vol 30 (2) ◽  
pp. 89-92
Author(s):  
Margaret Pooler ◽  
Hongmei Ma ◽  
David Kidwell-Slak
Keyword(s):  
United States ◽  
Tissue Culture ◽  
Cold Hardiness ◽  
Diploid Species ◽  
Pest Resistance ◽  
The United States ◽  
Tetraploid Species ◽  
Genetic Base ◽  
Flowering Cherry ◽  
New Cultivars

The United States National Arboretum has an ongoing flowering cherry (Prunus) breeding program aimed at broadening the genetic base of cultivated ornamental cherries by developing new cultivars with disease and pest resistance, tolerance to environmental stresses, and superior ornamental characteristics. Interploid crosses, specifically 2X × 4X, in ornamental Prunus would be beneficial in breeding because they could allow introgression of traits not available in the diploid germplasm (pest resistance, cold hardiness), and could result in the creation of seedless triploids that would not set nuisance fruit and possibly have extended bloom durations. This report documents successful hybridization of P. maackii (Manchurian or Amur cherry), a tetraploid species, with P. campanulata, P. ‘Umineko’, and P. maximowiczii, all diploid species. Chromosomes of one of these resulting triploid hybrids were successfully doubled using oryzalin in tissue culture to create a hexaploid plant.

scholarly journals Ex Vitro Rooting of American Chestnut Improves Acclimatization Survival and Plantlet Quality

2016 ◽  
Vol 34 (3) ◽  
pp. 75-79 ◽  
Author(s):  
Allison D Oakes ◽  
Tyler R. Desmarais ◽  
William A. Powell ◽  
Charles A. Maynard
Keyword(s):  
Tissue Culture ◽  
Keystone Species ◽  
Ex Vitro Rooting ◽  
Plant Production ◽  
Shoot Cultures ◽  
Ex Vitro ◽  
Regulatory Review ◽  
Survival Percentage ◽  
Virus Indexing

Tissue culture of plants has many applications, from producing genetically identical horticultural varieties, to production of secondary metabolites, to virus indexing, and most relevantly, developing novel traits by genetic transformation. Using Agrobacterium-mediated transformation on somatic embryos, blight-resistant American chestnuts [Castanea dentata (Marsh.) Borkh.] have been developed as shoot cultures in plant tissue culture. Rooting tissue-cultured shoots and acclimatizing the rooted plantlets are key steps in tree production. In this study, in vitro and ex vitro rooting methods were compared. The ex vitro method resulted in a lower initial rooting percentage but an overall higher survival percentage, resulting in higher potted plant production. The higher survival was likely due to partial acclimatization taking place before the plantlets were transplanted into potting mix. After 8 weeks, plantlets rooted via the ex vitro method were taller, and had more, and larger, leaves than the in vitro-rooted plantlets. These trees are currently in high demand for inoculation studies for federal regulatory review and eventually for restoration of this keystone species to its native habitat.

scholarly journals VARIATION AMONG PERENNIAL RYEGRASS x TALL FESCUE PLANTS FROM TISSUE CULTURE

1988 ◽  
pp. 81-86
Author(s):  
A.G. Scott ◽  
D.W.R. White
Keyword(s):  
Tissue Culture ◽  
Lolium Perenne ◽  
Perennial Ryegrass ◽  
Tall Fescue ◽  
Festuca Arundinacea ◽  
Chromosome Doubling ◽  
Chromosome Elimination ◽  
Intergeneric Hybrids ◽  
Hybrid Plants ◽  
Spontaneous Chromosome

Tissue culture was used in an attempt to obtain a fertile perennial ryegrass x tall fescue hybrid. Regenerated hybrid plants were found to be morphologically variable and contain extensive chromosome rearrangements. Spontaneous chromosome doubling had occurred as well as chromosome elimination. though no fertile hybrid plants have been obtained to date. Keywords: somaclonal variation, Lolium perenne, Festuca arundinacea, intergeneric hybrids

scholarly journals Mass Propagation of Feronia limonia L. through Tissue Culture

1970 ◽  
Vol 45 (1) ◽  
pp. 75-78 ◽  
Author(s):  
Shahina Islam ◽  
Mosfequa Zahan ◽  
Shahina Akter ◽  
Tanjina Akhtar Banu ◽  
Ahashan Habib ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Key Words ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Nodal Explants ◽  
Ms Medium ◽  
Ex Vitro ◽  
Mass Propagation

An efficient mass propagation method for Feronia limonia was developed from excised shoot tips and nodal explants of in vitro grown seedlings. Explants were cultured on MS medium with different conc. of NAA, Kn, IAA and BAP singly or in combinations. Highest number of micro shoots and better plant growth were obtained from these two explants on MS medium supplemented with 0.2 mg/l BAP alone. The regenerated shoots were successfully rooted on MS medium supplemented with 0.5 mg/l NAA. The in vitro raised plantlets were successfully established in soil following the formation of roots with 100% survivability under ex vitro condition. Key words: Feronia limonia; Mass propagation; Node; Shoot tips; Multiple shoot DOI: 10.3329/bjsir.v45i1.5186 Bangladesh J. Sci. Ind. Res. 45(1), 75-78, 2010

The first record of Iris setosa (Iridaceae) in Europe

Phytotaxa ◽  
2020 ◽  
Vol 437 (1) ◽  
pp. 32-38
Author(s):  
EUGENY V. BOLTENKOV ◽  
MARIJA Y. MENSHAKOVA ◽  
RAMZIYA I. GAINANOVA ◽  
ZINAIDA Y. RUMJANTSEVA
Keyword(s):  
North America ◽  
Russian Federation ◽  
Northeast Asia ◽  
First Record ◽  
Morphological Description ◽  
Murmansk Oblast ◽  
Original Material ◽  
Detection And Identification ◽  
First Time

Iris setosa is one of the widespread Iris species in Northeast Asia and North America. In 2018, it was found for the first time in Europe, on Kildin Island (Murmansk Oblast, Russian Federation). The presence of this plant on the island and its disjunct range are discussed. To facilitate further detection and identification, a general morphological description, information about the original material of the name I. setosa, and supporting illustrations are here provided.

Organic Tempering in Northeast Asia and Alaska

1958 ◽  
Vol 24 (2) ◽  
pp. 193-194 ◽  
Author(s):  
Chester S. Chard
Keyword(s):  
North America ◽  
New World ◽  
Northeast Asia ◽  
Northeastern Asia ◽  
Marked Contrast ◽  
The Status

Alaskan pottery techniques are distinguished by considerable use of a variety of organic tempering materials, in marked contrast to the rest of aboriginal North America. So pronounced a focus on the threshhold of the New World would suggest that the practice might have been borrowed from Asia. It will therefore be of interest to ascertain the status of organic tempers in northeastern Asia as compared with Alaska.

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