scholarly journals A Scale-up System for Lowbush Blueberry Micropropagation Using a Bioreactor

HortScience ◽  
2009 ◽  
Vol 44 (7) ◽  
pp. 1962-1966 ◽  
Author(s):  
Samir C. Debnath
Keyword(s):  
Large Scale ◽  
Scale Up ◽  
Basal Medium ◽  
Shoot Multiplication ◽  
Multiple Shoots ◽  
Multiplication Rate ◽  
Lowbush Blueberry ◽  
Isopentenyl Adenine ◽  
Bioreactor System

In an attempt to improve the micropropagation protocol for lowbush blueberry (Vaccinium angustifolium Ait.), a protocol using a bioreactor system combined with a semisolid gelled medium has been developed. Cultures of cultivar Fundy and two wild clones (‘NB1’ and ‘QB1’) were established in vitro on a gelled modified cranberry basal medium (BM) containing 5 μM zeatin or 10 μM N6-[2-isopentenyl]adenine. Multiple shoots were obtained within 8 weeks by transferring zeatin-induced shoots from the gelled BM to a bioreactor containing liquid BM with 1 to 4 μM zeatin. Genotypes differed significantly with respect to multiplication rate in liquid and gelled BM containing 1 μM zeatin with ‘NB1’ producing 8.5 ± 1.1 and 2.9 ± 0.3 shoots per explant in liquid and gelled media, respectively, after one subculture followed by ‘QB1’ (7.1 ± 0.6 and 2.6 ± 0.4 shoots per explant, respectively) and ‘Fundy’ (5.8 ± 0.4 and 2.0 ± 0.2 shoots per explant, respectively). With subculture, there was an increase of shoot multiplication rate for all genotypes. Bioreactor- and gelled medium-proliferated shoots were treated with 39.4 mm indole-3-butyric acid powder, rooted in a 2 peat:1 perlite (v/v) medium, plantlets acclimatized, and eventually established in the greenhouse with 64% to 74% rooting of microshoots and 90% to 99% survival of rooted shoots. Results obtained suggested the possibility of large-scale multiplication of lowbush blueberry shoots in bioreactors.

Developing a scale-up system for the in vitro multiplication of thidiazuron-induced strawberry shoots using a bioreactor

2008 ◽  
Vol 88 (4) ◽  
pp. 737-746 ◽  
Author(s):  
Samir C Debnath
Keyword(s):  
Large Scale ◽  
Scale Up ◽  
Adventitious Shoots ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Induction Medium ◽  
Fragaria Ananassa ◽  
Petiole Explants ◽  
Bioreactor System

The use of large-scale liquid cultures in a bioreactor system has the potential to resolve the manual handling of the various stages of micropropagation and increases shoot multiplication in vitro significantly compared with those cultured on semi-solid gelled medium. In an attempt to improve the micropropagation protocol for strawberry (Fragaria × ananassa Duch.), a procedure for the mass propagation of adventitious shoots regenerated from leaf, sepal and petiole explants of cultivar Bounty using a liquid medium-containing bioreactor system combined with gelled medium is described. Leaf disks, sepals and petiole halves produced multiple buds and shoots without an intermediary callus phase on 2-4 µM thidiazuron (TDZ)-containing shoot induction medium within 5-6 wk of culture initiation. TDZ supported rapid shoot proliferation at low concentrations (0.1 µM), but induced hyperhydricity in a bioreactor system. Bioreactor-multiplied hyperhydric shoots were transferred to gelled medium containing 2-4 µM zeatin, and produced normal shoots and root within 4 wk of culture. In vitro derived plantlets were acclimatized and eventually established in the greenhouse and in the field. Present results suggested the possibility of large-scale multiplication of strawberry shoots in bioreactors. Key words: Fragaria × ananassa, growth regulator, shoot regeneration, RITA® bioreactor

scholarly journals In Vitro Micropropagation of the Medicinal Plant Physalis angulata L.

2016 ◽  
Vol 8 (2) ◽  
pp. 161-163
Author(s):  
Owk ANIEL KUMAR ◽  
Songa RAMESH ◽  
Sape SUBBA TATA
Keyword(s):  
Large Scale ◽  
Leaf Disc ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Medicinal Herb ◽  
Root Induction ◽  
Survival Percentage ◽  
Physalis Angulata

Physalis angulata L. is an important medicinal herb. An efficient direct adventitious plant regeneration protocol was developed for large scale propagation using leaf disc as explants. The explants were cultured on MS basal medium supplemented with 0.25-3.0 mg/L 6-benzyl amino purine (BAP) for primary shoot proliferation. Inclusion of indole-3-acetic acid (IAA) and gibberellic acid (GA3) in the culture medium along with BAP promoted a higher rate of shoot multiplication. The maximum number of shoots was produced in MS + BAP (1.0 mg/L) + IAA (0.5 mg/L) + GA3 (0.20 mg/L) after the third subculture. An average of 152.8 ± 0.40 shoots were produced from each leaf disc. For root induction the shootlets were transferred to MS medium supplemented with different concentrations of indole-3-butyric acid (IBA). The highest percentage of root induction was observed in 1.0 mg/L (IBA). Rooted plants were successfully established in the soil after hardening. The survival percentage of rooted plants on soil was found to be 85%. This result will facilitate the conservation and propagation of the important medicinal herb Physalis angulata L.

scholarly journals IMPROVED MULTIPLICATION OF MATURE HAZELNUT (CORYLUS AVELLANA L.) IN VITRO

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 693b-693
Author(s):  
Xiaoling Yu ◽  
Barbara M. Reed
Keyword(s):  
Carbon Sources ◽  
Basal Medium ◽  
Shoot Multiplication ◽  
Multiplication Rate ◽  
Shoot Cultures ◽  
Mature Trees ◽  
Medium Carbon ◽  
Prolonged Culture ◽  
Corylus Avellana L

Multiplication and elongation of shoot cultures established from mature trees of hazelnut cvs. Nonpareil and Tonda Gentile Romana were affected by changes in basal medium, carbon source and concentration, cytokinin and agar concentration. Explants on DKW medium produced significantly more shoots than those on Anderson medium or modified woody plant medium for chestnut. Explants on DKW medium with 3% glucose or fructose gave more and longer shoots than those with the other carbon sources. Cytokinins 6 benzylaminopurine (BA) and zeatin were more effective in producing shoots than kinetin and 2iP. On BA supplemented medium, the best multiplication rate was obtained with 1.5 - 2.0 mg/l. Explants grown on 0.4% agar produced more shoots than those on 0.6%, however, prolonged culture on 0.4% agar caused vitrification of lower parts of the plants. Shoot multiplication rates of these two cultivars were similar, but `Nonpareil' produced longer shoots than `Tonda Gentile Romana'.

scholarly journals In Vitro Regeneration of Vitex negundo L., a Woody Valuable Medicinal Plant through High Frequency Axillary Shoot Proliferation

1970 ◽  
Vol 43 (3) ◽  
pp. 345-352 ◽  
Author(s):  
Farhana Afroz ◽  
AKM Sayeed Hassan ◽  
Laila Shamroze Bari ◽  
Rebeka Sultana ◽  
John Liton Munshi ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Large Scale ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Multiple Shoots ◽  
Nodal Segments ◽  
Axillary Shoot ◽  
Vitex Negundo ◽  
Axillary Shoot Proliferation

An efficient protocol was established for rapid and large scale propagation of woody aromatic medicinal plant Vitex negundo L. by in vitro shoot multiplication from shoot tips and nodal segments of mature plant. Of the four different growth regulators BA, Kn, GA3, NAA and coconut water, MS fortified with BA 1.0 mg/l was found to be the most effective for inducing multiple shoots from nodal explants. The percentage (96%) of shoot multiplication per node (21.83) was highest up to second subculture passages, after which there was a gradual decline in shoot development. Best rooting was induced (93%) in excised shoots on half strength MS medium supplemented with an optimal combination of NAA (0.3 mg/l). Soil, compost and sand (1:1:1) mixture was the most suitable planting substrate for hardening. The survival rate was 80% and the regenerated plants were successfully transferred to the soil.Key words: Vitex negundo, Medicinal plant, Shoot proliferation, Micropropagation, RegenerationDOI = 10.3329/bjsir.v43i3.1149Bangladesh J. Sci. Ind. Res. 43(3), 345-352, 2008

In vitro propagation of Camellia fascicularis: a plant species with extremely small populations

2020 ◽  
Vol 100 (2) ◽  
pp. 202-208
Author(s):  
Mengting Wang ◽  
Guiliang Zhang ◽  
Peiyao Xin ◽  
Yun Liu ◽  
Bin Li ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Large Scale ◽  
In Vitro Regeneration ◽  
Germplasm Conservation ◽  
Shoot Multiplication ◽  
Shoot Tips ◽  
Naphthalene Acetic Acid ◽  
Multiplication Rate ◽  
Ms Medium

Camellia fascicularis is an endangered evergreen ornamental plant with pale yellow flowers. An efficient and reproducible in vitro regeneration method is required for its large-scale propagation and germplasm conservation. In this study, one axillary bud per nodal stem was obtained from C. fascicularis cultured on Murashige & Skoog (MS) medium containing 0.1 mg L−1 indole-3-acetic acid (IAA) combined with 1.0 mg L−1 6-benzylaminopurine (BA). Axillary buds from the stem segments were transferred to modified woody plant medium (WPM) supplemented with 3.0 mg L−1 BA in combination with 0.3 mg L−1 IAA for multiplication, thereby resulting in a high shoot multiplication rate of 6.8. Multiple shoots were divided into nodal stems and shoot tips and were induced to root. The shoot tips were induced to root by culturing on one-half MS medium supplemented with 2.0 mg L−1 indole-3-butyric acid (IBA) in combination with 0.3 mg L−1 α-naphthalene acetic acid (NAA), which resulted in 76.0% rooting efficiency with 2.3 roots per shoot. The optimal hormone ratio for inducing rooting of nodal stems was 1.0 mg L−1 IBA in combination with 2.0 mg L−1 NAA, which resulted in 72.7% rooting efficiency with 1.7 roots per nodal stem. These two rooted plantlets were successfully acclimatized and established in a greenhouse.

scholarly journals Micropropagation of Wetland Plants: Sagittaria latifolia

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 547B-547
Author(s):  
Michael E. Kane ◽  
Charles Lane
Keyword(s):  
Basal Medium ◽  
Shoot Multiplication ◽  
High Sensitivity ◽  
Wetland Plants ◽  
Environmental Damage ◽  
Multiplication Rate ◽  
Mineral Salts ◽  
Sagittaria Latifolia ◽  
Wetland Species

Many wetland plant species used for aquascaping and wetland revegetation projects are collected from donor wetland sites for planting elsewhere. Increased demand for wetland plants has lead to over-collection and subsequent environmental damage to these donor sites. Micropropagation provides an ecologically sound alternative to field collection and allows for production of under utilized wetland species and genotypes that are either slow-growing or difficult to propagate using conventional methods. Sagittaria latifolia Willd. (Duck-potato), a rhizomatous herbaceous wetland species, was established in vitro from surface-sterilized lateral and terminal rhizome shoot-tips cultured in liquid basal medium consisting of half-strength Murashige and Skoog mineral salts, 0.56 mM myo-inositol and 1.2 μM thiamine supplemented with 87.6 mM sucrose. Prior to multiplication, responsive Stage I cultures were indexed for cultivable bacteria and fungi. Shoot multiplication occurred in vitro through formation of multiple node rhizomes bearing terminal shoots. Duck-potato exhibited a high sensitivity to relatively low benzyladenine (BA) levels. Maximum rhizome and shoot production occurred from single shoot explants initially cultured on agar-solidified BM supplemented with 4.0 μM BA for 28 days. However, repeated subculture on BM supplemented with greater than 2.5 μM BA resulted in increased mortality, reduction in multiplication rate, or production of dormant corms. Consistent shoot multiplication (four to five shoots/explant) was possible in the presence of 1.5 μM BA. Maximum (100%) acclimatization and rooting was attained by direct sticking of Stage II microcuttings in soilless growing medium contained in 38 cell plugs. Production of salable plants bearing multiple rhizomes was possible within 6 weeks post-transplant. Preliminary observations indicate that corm formation in Sagittaria latifolia may be mediated by photoperiod.

scholarly journals 027 MULTIPLICATION OF ROSE SPECIES IN VITRO

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 431d-431
Author(s):  
Yan Ma ◽  
David H. Byrne ◽  
Jing Chen ◽  
Amanda Byrne
Keyword(s):  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Multiple Shoots ◽  
Naphthalene Acetic Acid ◽  
Good Growth ◽  
Lateral Buds ◽  
Half Strength ◽  
The Media

Several rose species (Rosa rugosa, R. wichuraiana, R. setigera, R. laevigata, R. banksiae, R. roxburghii, R. odorata and hybrids) were employed to establish the appropriate nutrient media for shoot multiplication and root initiation of cultured shoots and to describe a procedure for the successful transfer to soil of plants obtained in vitro. Cultured shoot tips and lateral buds from different genotypes proliferated multiple shoots on a basal medium (MS salt, vitamins, glycine, sucrose and agar) supplemented with 0mg/l to 6mg/l 6-benzylamino purine (BA) and 0mg/l to 0.5 mg/l naphthalene acetic acid (NAA). Most rose species cultured in a modified MS medium supplemented with 2mg/l BA showed good growth and shoot proliferation. The buds nearest the apex exhibited the slowest rate of bud development. Root development was enhanced and shoot development inhibited by lowering the concentration of MS salts to quarter- and half-strength. With difficult-to-root species, rooting was improved by supplementing the media with auxin or giving them 3-7days of dark treatment.

scholarly journals THE INFLUENCE OF GROWTH REGULATORS ON DAHLIA PROPAGATION IN TISSUE CULTURE AND ACCLIMATIZATION OF PLANTS IN ex vitro CONDITIONS

2019 ◽  
Vol 18 (5) ◽  
pp. 49-61
Author(s):  
Barbara Marcinek ◽  
Marzena Parzymies ◽  
Monika Poniewozik ◽  
Danuta Kozak ◽  
Wojciech Durlak
Keyword(s):  
Shoot Multiplication ◽  
Multiplication Rate ◽  
Ex Vitro ◽  
Fresh Weight ◽  
Flower Stalk ◽  
Axillary Shoots ◽  
First Order ◽  
Isopentenyl Adenine ◽  
Consequent Effect

The aim of work was to evaluate the influence of cytokinins on dahlia propagated in vitro and their consequent effect on acclimatization. Plant material were shoot tips and nodes. From three cytokinins, benzyladenine, kinetin and 2-isopentenyl-adenine, only BA effectively stimulated shoot multiplication from axiliary buds. The highest multiplication rate was obtained from nodes in presence of 0.25–0.5 mg·dm–3 BA. Higher concentrations shortened internodes and decreased leaf blades and growth of callus. 1 mg·dm–3 of KIN and 2iP positively influenced shoots growth and size of leaves. Gibberellic acid (GA3) used with BA increased the number of axillary shoots. The best quality shoots and the highest multiplication rate were obtained when 2 mg·dm–3 BA was used with 5 mg·dm–3 GA3. Cytokinins affected rooting and acclimatization ex vitro. Dahlias shoots multiplicated in presence of 1 mg·dm–3 KIN or 2iP rooted faster in the soil and 100% survived in field, while those from 1 mg·dm–3 BA media rooted slowly, had shorter shoots and only 60% survived. Plants bloomed after 11–12 weeks in the field. Dahlia plants that had been multiplicated in presence of KIN had a bigger diameter and fresh weight in the field. BA and 2iP positively influenced flowers diameter, length of flower stalk and a number of first order shoots.

scholarly journals Develope Micro clonal -propagation protocol for Oxytenanthera abyssinica A.Rich. Munro to large scale micro-propagation

2020 ◽  
Author(s):  
Adugnaw Admas ◽  
Berhane Kidane ◽  
Melaku Admasu ◽  
Tesaka Misga
Keyword(s):  
Large Scale ◽  
Shoot Multiplication ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Shoot Initiation ◽  
Oxytenanthera Abyssinica ◽  
Micro Propagation ◽  
Viable Seeds ◽  
Propagation Methods

ABSTRACTIn Ethiopia, Oxytenanthera abyssinica A.Rich. Munro has varies economic importance. However, conventional propagation methods of O. abyssinica are generally inefficient due to their low multiplication rate, time consuming, labor intensive, and too costly. The objective of this study was to develop a protocol for mass micropropagation of O. abyssinica through seed culture. Murashige and Skoog (MS) medium augmented with 6-Benzylaminopurine (BAP) was used for shoot initiation and multiplication. For in vitro rooting, MS medium supplemented with 3-Indole –butric acid (IBA) was used.In shoot initiation experiment all viable seeds were proliferated in 5-7 days of culturing. In shoot multiplication at 0.004 g/L BAP was Sucssefuly shoot multiplied, also best root responding were found at 0.005 g/l IBA.The present optimized protocol enables for any acters who needs large numbers of low land bamboo seedling for industery, small and micro enterprize or for reafforestation programms.

scholarly journals Micropropagation of banana (Musa spp) using temporary immersion bioreactor system

2021 ◽  
Vol 12 (2) ◽  
pp. 197-200
Author(s):  
M.M. Abdulmalik ◽  
I.S. Usman ◽  
A.U. Nasir ◽  
L.A. Sani
Keyword(s):  
Large Scale ◽  
Shoot Multiplication ◽  
Cost Effective ◽  
Scale Production ◽  
Multiplication Rate ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Cost Effective Method ◽  
Bioreactor System ◽  
Planting Materials

Banana is an important crop in the tropics which possess the potential for commercial production in Nigeria. Large scale production requires large volume of planting materials which may be difficult to obtain using conventional methods of propagation. Temporary immersion bioreactor system (TIBs) is a cost effective method for micropropagation of plants. The present study was carried out to develop an efficient method for rapid multiplication of banana using temporary immersion bioreactor system (TIBs). Banana microshoots were regenerated from young suckers obtained from field grown plants using conventional plant tissue culture. Microshoots of 2cm length were used as explants for multiplication in temporary immersion bioreactor system. Ten (10) explants were cultured in bioreactor bottles containing Murashinge and Skoog (MS) liquid media supplemented with different concentrations of 6-bezylaminopurine (BAP) with or without 250mg/L Activated Charcoal (AC). Results showed that explants cultured in media supplemented with 2 mg/L or 1mg/L BAP without AC gave the highest shoot multiplication rate of 900% and 800%, respectively compared to hormone free media. Production of competent plants (plants ready for ex vitro establisment) were however, influenced by the presence of AC and the highest percentage of competent plants (80%) were produced when media was fortified with 1mg/L BAP+ 250mg AC. Regenerated plants were successfully established in the field and were morphologically normal and fertile.

Sign in / Sign up

Export Citation Format

Share Document