scholarly journals Develope Micro clonal -propagation protocol for Oxytenanthera abyssinica A.Rich. Munro to large scale micro-propagation

2020 ◽  
Author(s):  
Adugnaw Admas ◽  
Berhane Kidane ◽  
Melaku Admasu ◽  
Tesaka Misga
Keyword(s):  
Large Scale ◽  
Shoot Multiplication ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Shoot Initiation ◽  
Oxytenanthera Abyssinica ◽  
Micro Propagation ◽  
Viable Seeds ◽  
Propagation Methods

ABSTRACTIn Ethiopia, Oxytenanthera abyssinica A.Rich. Munro has varies economic importance. However, conventional propagation methods of O. abyssinica are generally inefficient due to their low multiplication rate, time consuming, labor intensive, and too costly. The objective of this study was to develop a protocol for mass micropropagation of O. abyssinica through seed culture. Murashige and Skoog (MS) medium augmented with 6-Benzylaminopurine (BAP) was used for shoot initiation and multiplication. For in vitro rooting, MS medium supplemented with 3-Indole –butric acid (IBA) was used.In shoot initiation experiment all viable seeds were proliferated in 5-7 days of culturing. In shoot multiplication at 0.004 g/L BAP was Sucssefuly shoot multiplied, also best root responding were found at 0.005 g/l IBA.The present optimized protocol enables for any acters who needs large numbers of low land bamboo seedling for industery, small and micro enterprize or for reafforestation programms.

In vitro propagation of Camellia fascicularis: a plant species with extremely small populations

2020 ◽  
Vol 100 (2) ◽  
pp. 202-208
Author(s):  
Mengting Wang ◽  
Guiliang Zhang ◽  
Peiyao Xin ◽  
Yun Liu ◽  
Bin Li ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Large Scale ◽  
In Vitro Regeneration ◽  
Germplasm Conservation ◽  
Shoot Multiplication ◽  
Shoot Tips ◽  
Naphthalene Acetic Acid ◽  
Multiplication Rate ◽  
Ms Medium

Camellia fascicularis is an endangered evergreen ornamental plant with pale yellow flowers. An efficient and reproducible in vitro regeneration method is required for its large-scale propagation and germplasm conservation. In this study, one axillary bud per nodal stem was obtained from C. fascicularis cultured on Murashige & Skoog (MS) medium containing 0.1 mg L−1 indole-3-acetic acid (IAA) combined with 1.0 mg L−1 6-benzylaminopurine (BA). Axillary buds from the stem segments were transferred to modified woody plant medium (WPM) supplemented with 3.0 mg L−1 BA in combination with 0.3 mg L−1 IAA for multiplication, thereby resulting in a high shoot multiplication rate of 6.8. Multiple shoots were divided into nodal stems and shoot tips and were induced to root. The shoot tips were induced to root by culturing on one-half MS medium supplemented with 2.0 mg L−1 indole-3-butyric acid (IBA) in combination with 0.3 mg L−1 α-naphthalene acetic acid (NAA), which resulted in 76.0% rooting efficiency with 2.3 roots per shoot. The optimal hormone ratio for inducing rooting of nodal stems was 1.0 mg L−1 IBA in combination with 2.0 mg L−1 NAA, which resulted in 72.7% rooting efficiency with 1.7 roots per nodal stem. These two rooted plantlets were successfully acclimatized and established in a greenhouse.

scholarly journals A Practical and Efficient Method for the Micropropagation of Japanese Cherry (Prunus sp.)

2019 ◽  
Vol 1 (4) ◽  
pp. 261-269
Author(s):  
Thuy Linh Thi Nguyen ◽  
Ngoc Thi Pham ◽  
Thao Thi Ninh ◽  
Phuong Thao Thi Nguyen
Keyword(s):  
Climate Adaptation ◽  
Shoot Multiplication ◽  
Good Growth ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Rice Husks ◽  
Shoot Initiation ◽  
Primary Explants ◽  
Planting Materials

This study was conducted to establish the procedure for in vitro propagation of Japanese cherry (Prunus sp.) to produce large quantity of plantlets and initial planting materials for climate adaptation research of this plant in Hanoi. Single nodal stems were used as the primary explants and initially produced shoots on MS medium supplemented with 1 mg L-1 BA. The highest shoot multiplication rate (9.57 times) was obtained on MS medium containing 1 mg L-1 BA and 0.25 mg L-1 a-NAA after 8 weeks of culture. 100% of the shoots produced roots with a mean of 10.10 roots per plant within 4 weeks on ½ MSM medium with 4 mg L-1 IBA. The survival rate of in vitro derived plantlets after a 6 to 7-week-period of rooting during acclimatization using a soil: coco peat: smoked rice husks (2:2:1, v/v/v) substrate was 100% and acclimatized plantlets showed good growth and development. This is the first report on a practical and efficient in vitro multiplication protocol for Japanese cherry in Vietnam, starting from shoot initiation to establishment of plants under greenhouse conditions.

scholarly journals In vitro Propagation of Banana (Musa paradisiaca L.) Plant Using Shoot Tip Explant

2021 ◽  
Vol 9 (12) ◽  
pp. 2339-2346
Author(s):  
Girmay Mekonen ◽  
Meseret Chimdessa Egigu ◽  
Manikandan Muthsuwamy
Keyword(s):  
Shoot Multiplication ◽  
Shoot Tip ◽  
Root Induction ◽  
Ms Medium ◽  
Shoot Initiation ◽  
Half Strength ◽  
Banana Variety ◽  
Propagation Methods ◽  
Number Of Shoots

Banana is a fruit crop which has high demand in Ethiopia, but its production is constrained by lack of disease free planting material with conventional propagation methods. For shoot initiation, shoot tip explants were cultured on MS medium supplemented with 0.5, 1.0, 1.5 and 2.0 mg/L BAP. Similarly, MS medium supplemented with BAP at 1.0, 1.5, 2.0 mg/L in combination with IBA at 0.25 and 0.50 mg/L were used for shoot multiplication. Half- strength MS medium augmented with IBA at 1.0, 2.0, 3.0 and 4.0 mg/l were used for root induction. MS medium without PGRs were used as controls. Finally, hardening of the in vitro derived plantlets was carried out in green house both in the primary and secondary acclimatization stages. Results showed that the highest shoot initiation percent (93.40%), highest mean number of shoots per explant (4.67) and lesser day for shoot induction (11.00) were observed in explant cultured on MS + 1.0 mg/L BAP. With shoot multiplication, highest shooting percent (92.60%), maximum number of shoots (7.67) and highest shoot length (5.27 cm) were recorded on MS + 1.5 mg/L BAP + 0.5 mg/L IBA. The highest rooting percent (93.40%), maximum root number per shoot (7.67) and highest root length (11.00 cm) were found on a half strength MS medium + 2.0 mg/L IBA. The survival rate of plantlets were 96.00% in coco peat substrate in primary acclimatization and 97.92% in forest soil, sand and manure substrates mixed at 3:2:1 ratio in secondary acclimatization. Overall, the result showed that the PGRs type, concentrations and combinations used are effective for mass propagation of banana variety studied in this experiment.

scholarly journals EMBRYO CULTURE AND IN VITRO CLONAL PROPAGATION OF OAK (Quercus aegilops L.)

2020 ◽  
Vol 51 (1) ◽  
Author(s):  
Fadladeen & Toma
Keyword(s):  
Embryo Culture ◽  
Germination Rate ◽  
Shoot Multiplication ◽  
Ms Medium ◽  
Suitable Alternative ◽  
Culture Techniques ◽  
Initiation Stage ◽  
Propagation Methods ◽  
Better Than

An attempt was done to develop a micropropagation protocol for oak using embryo culture. Oak is considered a hard-to-root woody plant by conventional propagation methods, that’s why using tissue culture techniques is a very suitable alternative method. For oak embryo culture, WPM was used and found to be better than MS medium for embryo germination which gave 66.13%. As well as adding of GA3 to the medium improved the germination rate of embryos (43.25% and 82.25 %). At initiation stage, WPM was used and found to be the best medium by giving the highest number of shoots/ explant which was 1.80, the highest number of leaves (15.17 leaves/ explant) and the longest shoots (1.42 cm) followed by MS medium then GD which gave the lowest parameters which gave 0.98 shoots/ explant, 7.20 leaves/ explant and 1.06 cm shoot length. At shoot multiplication stage, BA was better than Kinetin for multiplication of oak explants. The addition of BA at 3 mg.l-l gave the highest number of shoot and leaves which were 3.33 and 26.11 respectively. The longest shoots were achieved when 4.5 mg.l-l of BA was used. Furthermore, kinetin at 3 and 4.5 mg-l gave the lowest parameters which were 1 cm in length and 1.54 leaves/ explant. For rooting stage, NAA was better than IAA in giving better parameters and rooting percentage. The highest number of roots and rooting percentage were achieved when 1 mg.l-l was added by giving 6 roots/ explant and 100% rooting percentage. While the longest roots were achieved when 0.5 mg.l-l of NAA was used (3.67 cm) followed by 1.5 mg.l-l IAA which gave 3.55 roots/ explant with rooting percentage 90%. The produced plantlets were successfully acclimatized and transferred to the open-air conditions with a rate reached 85%.

scholarly journals The effect of BA and GA3 on the shoot multiplication of in vitro cultures of Polish wild roses

2011 ◽  
Vol 23 (2) ◽  
pp. 145-149 ◽  
Author(s):  
Bożena Pawłowska
Keyword(s):  
Relative Humidity ◽  
Ornamental Plants ◽  
Shoot Multiplication ◽  
In Vitro Cultures ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Naturally Occurring ◽  
Dog Rose ◽  
The University

The effect of BA and GA3on the shoot multiplication ofin vitrocultures of Polish wild rosesThe experiment was conducted using five species of roses naturally occurring in Poland:Rosa agrestis(fieldbriar rose),R. canina(dog rose),R. dumalis(glaucous dog rose),R. rubiginosa(sweetbriar rose), andR. tomentosa(whitewooly rose), from thein vitrocollection of the Department of Ornamental Plants of the University of Agriculture in Kraków. We examined the effect of cytokinin BA (1-10 μM) added to an MS medium (Murashige and Skoog 1962) on auxiliary shoot multiplication. The second group of test media contained BA (1-5 μM) and gibberellin GA3(0.3-1.5 μM). The cultures were maintained at a phytotron temperature of 23/25°C (night/day), 80% relative humidity, with a 16-hour photoperiod and PPFD of 30 μmol m-2s-1, and cultured in five-week cycles. The highest multiplication rate was obtained forR. caninaandR. rubiginosa(4.1 shoots per one explant) andR. dumalis(2.9 shoots per one explant), when shoots were multiplied on an MS medium supplemented with 1 μM BA and 1.5 μM GA3. Multiplication was the weakest inRosa tomentosaindependent of the medium used.

scholarly journals Process of Establishment And In Vitro Development of Simaba Cedron Planch Seedlings. (Simaroubaceae)

2020 ◽  
Vol 8 (8) ◽  
pp. 748-759
Author(s):  
Marcia Santos de Freitas Lira ◽  
Simone Da Silva ◽  
Fábio Leandro Calderaro ◽  
Jandecy Cabral Leite
Keyword(s):  
Large Scale ◽  
Culture Media ◽  
Physical Space ◽  
Scale Production ◽  
Multiplication Rate ◽  
Large Scale Production ◽  
Snake Bites ◽  
Propagation Methods ◽  
Vitro Development

Simaba cedron, popularly known as "cedron", is largely used for fever and snake bites. Its seeds are used in the treatment of stomach problems and liver infections. The fruits are used for the treatment of pain and malaria while its bark is an antispasmodic. Simaba cedron is generally propagated through seeds, but with limited success, as the low viability of same restricts its propagation. In view of such difficulty, it becomes necessary the study for adequate conditions for the large scale production of these seedlings. Being it known that in several species, the use of micropropagation has made it possible to obtain a large amount of disease-free and more homogeneous seedlings, in reduced time and physical space, in comparison with conventional propagation methods, the objective of this work was to analyze the effect of two culture media on the production of aseptic parent plants as a first step in the development of a micropropagation protocol for Simaba cedron. The seeds were collected from a matrix plant located in the Amazon Biotechnology Center (CBA), in Manaus/AM. The experiment was installed at the Vegetable Tissue Culture Laboratory, where the  explants were desinfected and grown in culture medium  according to Murashige & Skoog (MS) and in Wood Plant Medium (WPM), during 60 days. The disinfestation rate obtained was 75% and, of the disinfested seeds, 100% germinated. The cultivation medium that was more favorable to the cultivation of simaba was the MS, where the multiplication rate was of 8.0: 1, whose seedlings reached, in average, 4.8 cm and 75% of rooting.

scholarly journals Micropropagation of Baptisia `Purple Smoke'

HortScience ◽  
1999 ◽  
Vol 34 (2) ◽  
pp. 353-354 ◽  
Author(s):  
James R. Ault ◽  
Kayri Havens
Keyword(s):  
Plant Growth Regulator ◽  
Potassium Salt ◽  
Shoot Growth ◽  
Basal Medium ◽  
Shoot Multiplication ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Single Node ◽  
Shoot Initiation

Shoot explants from actively growing, greenhouse-maintained plants of Baptisia `Purple Smoke' were cultured in vitro for shoot initiation on Murashige and Skoog (MS) basal medium containing vitamins and supplemented with 30 g·L–1 sucrose, 8.87 μm BA, and 4.14 μm K-IBA. All subsequent media were supplemented with 2.47 mm NaH2PO4 to enhance shoot growth. Single-node explants were subcultured for shoot multiplication on MS medium with either no plant growth regulator or with 2.22, 4.44, 8.87, 17.74, or 35.48 mm BA in combination with 0.0 or 4.14 μm K-IBA. Explants produced a maximum of 4.1 shoots on the medium with 2.22 μm BA. Shoots rooted on all concentrations of K-IBA (2.07, 4.14, 10.36, or 20.72 μm) and K-NAA (2.23, 4.46, 11.15, or 22.29 μm) tested. Maximum rooting was 100% on MS medium with 11.15 μm K-NAA; however, this treatment induced copious stem callusing. Rooted shoots were greenhouse-acclimatized for 2.5 weeks. Overall survival was 86%. For optimal rooting and subsequent acclimatization, treatment with 2.23 μm K-NAA is recommended; this resulted in 83% rooting and 87% acclimatization. Chemical names used: N6 benzyladenine (BA); potassium salt of indole-3-butyric acid (K-IBA); potassium salt of 1-naphthalene acetic acid (K-NAA).

Rapid Micropropagation Protocol of Mucuna pruriens var. utilis using Cotyledonary Node Explant: A Cultivated Medicinal Edible Legume

2021 ◽  
Author(s):  
Sanjay Kumar Madkami ◽  
Arpita Moharana ◽  
Durga Prasad Barik
Keyword(s):  
Seed Germination ◽  
Large Scale ◽  
Shoot Multiplication ◽  
Cotyledonary Node ◽  
Production Method ◽  
Commercial Production ◽  
Mucuna Pruriens ◽  
Garden Soil ◽  
Ms Medium

Background: The presence of L-dopa coupled with rich protein and amino acid marked Mucuna pruriens var. utilis as an important under-utilised legume. Therefore, it is useful to develop a method for large-scale multiplication for commercial production. Method: Tissue culture technology is successfully utilized in propagation of plants with poor and uncertain response to conventional propagation. Murashige and Skoog’s (MS) medium without any Plant Growth Regulators (PGRs) was used for seed germination and with PGRs for shoot and root multiplication.Result: Highest 95% seed germination was found in fresh seeds at 7-8 days of culture. Shoot multiplication percentage was found to be 100% with highest c.a. 21.1 shoots with an average 4.8 cm shoot length on MS + BAP 1.5 mg L-1 per 10 days old cotyledonary node explant. A total c.a. 144 shoots were harvested after 3rd harvest of the mother cotyledonary node with two whole cotyledons at day 70. Rooting was best induced in in vitro derived shoots on MS medium without any PGRs and plantlets were acclimatized in sand and soil (1:1) and established in pot with garden soil. 

scholarly journals Influence of Additives on Enhanced In vitro Shoot Multiplication of Orthosiphon aristatus (Blume) Miq.

2013 ◽  
Vol 5 (3) ◽  
pp. 338-345 ◽  
Author(s):  
Swarna JAYAKUMAR ◽  
Ravindhran RAMALINGAM
Keyword(s):  
Large Scale ◽  
Shoot Multiplication ◽  
Ms Medium ◽  
Morphological Variations ◽  
Different Types ◽  
Mother Plants ◽  
Half Strength ◽  
Different Parts ◽  
Number Of Shoots

Orthosiphon aristatus is a valuable medicinal plant and different parts of the plant are pharmaceutically used for the treatment of various diseases. The present study was designed to develop an efficient protocol for micropropagation of O. aristatus from nodal explants and to study the influence of additives on the enhancement of the number of shoots per explant. Among the different types of additives used, 10% coconut water and 30 mg/L glutamine added to Murashige and Skoog’s (MS) medium supplemented with 1.0 mg/L 6-benzyl amino purine (BAP) and 0.5 mg/L kinetin (KIN) was found to be most effective. Maximum number of shoots (44.07 ± 0.38) with 100% shooting response and shoot length of 7.47 ± 0.10 cm was recorded. In vitro rooting of the microshoots was achieved on half-strength MS medium containing 0.5 mg/L indole-3-butyric acid (IBA), producing an average of 30.27 ± 0.36 roots and 6.02 ± 0.20 cm root length. The rooted shoots were acclimatized with 100% survival rate on coco pith: soil (3:1) planting substrate and was successfully transferred to field conditions. The hardened plants exhibited homogeneity and no morphological variations were observed among the regenerants and the mother plants. Thus, the procedure described is a quick and reliable method which could be applied for efficient large-scale propagation, genetic transformation assays and secondary metabolite production.

scholarly journals In Vitro Regeneration of Grass Pea (Lathyrus sativus L.) from Cotyledonary Node Explants

2017 ◽  
Vol 5 (2) ◽  
pp. 57 ◽  
Author(s):  
Diriba Tesfaye ◽  
Kassahun Bantte ◽  
Tewodros Tadesse
Keyword(s):  
In Vitro Regeneration ◽  
Shoot Multiplication ◽  
Full Potential ◽  
Ms Medium ◽  
Regeneration Capacity ◽  
Grass Pea ◽  
In Vitro Techniques ◽  
Shoot Initiation ◽  
Shoot Number

Full potential of grass pea has not been utilized because of the presence of the neurotoxin amino acid β-N-oxalyl-L-αβ -diaminopropionic acid (ODAP/BOAA). Conventional breeding and other approaches have not been successful in reducing the toxin. Integration of in vitro techniques can contribute significantly to meet the challenge. Therefore, this study was carried out to evaluate the in vitro regeneration capacity of grass pea genotypes. Shoot initiation, multiplication and rooting of IVAT-LS-690 were conducted using completely randomized design with five replications. Genotypes were treated with BAP and NAA for shoot initiation while BAP and Kn Combination were used for multiplication. Different concentrations of IBA and IAA were used for rooting. Shoot proliferation percentage was the highest (100%) for IVAT-LS-690,on Murashige and Skoog (MS) medium augmented with 2.0 mg/l BAP +0.1 mg/lNAA.For in vitro shoot multiplication, best results were obtained on concentrations of 3mg/l BAP+1mg/l Kn with maximum shoot number per explants (11.5). High number of roots per shoot (6) and percent of rooted shoot (86.66%) were obtained from ½ MS medium supplemented with 0.5 mg/l IBA. This study inferred that both genotype and BAP levels play a crucial role for shoot regeneration capacity and the optimum hormonal combination for grass pea is genotype specific.

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