scholarly journals Virulence Phenotypes on Chili Pepper for Phytophthora capsici Isolates from Michoacán, Mexico

HortScience ◽  
2019 ◽  
Vol 54 (9) ◽  
pp. 1526-1531
Author(s):  
Alfredo Reyes-Tena ◽  
Arturo Castro-Rocha ◽  
Gerardo Rodríguez-Alvarado ◽  
Gerardo Vázquez-Marrufo ◽  
Martha Elena Pedraza-Santos ◽  
...  
Keyword(s):  
New Mexico ◽  
Disease Severity ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Phytophthora Capsici ◽  
Inbred Lines ◽  
Chili Pepper ◽  
Economic Losses ◽  
Post Inoculation ◽  
Resistant Cultivars

Phytophthora blight of vegetables caused by Phytophthora capsici causes significant economic losses in production of Solanaceae and Cucurbitaceae crops in Mexico. The development of universal resistant chili pepper cultivars is challenging due to the diverse virulence phenotypes produced by P. capsici. The objective of the study was to characterize the diversity of phenotypic interactions for P. capsici isolates recovered from production fields in Michoacán, Mexico, to facilitate the development of resistant cultivars. Virulence phenotypes were characterized for 12 isolates of P. capsici using 26 Capsicum annuum New Mexico Recombinant Inbred Lines (NMRILs) in greenhouse conditions. Criollo de Morelos CM-334 and California Wonder were used as resistant and susceptible controls, respectively. Seedlings at the four to eight true leaf stage were inoculated with 10,000 zoospores per seedling and disease severity was evaluated at 20 days post-inoculation. Two of the P. capsici isolates did not infect any pepper host even though the isolate was less than a year old. The 10 virulent isolates were designated in 10 virulence phenotypes. The information generated by this study is of utmost importance for efforts of producing resistant cultivars specific for Michoacán producers.

scholarly journals Physiological Race Characterization of Phytophthora capsici Isolates from Several Host Plant Species in Brazil Using New Mexico Recombinant Inbred Lines of Capsicum annuum at Two Inoculum Levels

2012 ◽  
Vol 137 (6) ◽  
pp. 421-426 ◽  
Author(s):  
Cláudia S. da Costa Ribeiro ◽  
Paul W. Bosland
Keyword(s):  
New Mexico ◽  
Capsicum Annuum ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Phytophthora Capsici ◽  
Screening Method ◽  
Inbred Lines ◽  
Screening Methods ◽  
Resistant Cultivars ◽  
Physiological Races

Phytophthora blight, caused by Phytophthora capsici, is one of the most destructive diseases worldwide that affects Capsicum pepper. Attempts to provide universally resistant cultivars has been unsuccessful, which may be the result of the use of different resistance sources, pathotypes, or races of P. capsici isolates and screening techniques. The screening method used at Embrapa Vegetables in Brazil to detect resistance to P. capsici in Capsicum was compared with the screening method used at New Mexico State University. Both screening methods produced similar and consistent results when a range of P. capsici isolates were used. It was concluded that either method can successfully differentiate resistant and susceptible individuals. In addition, 20 P. capsici isolates from Brazil were characterized for virulence using a subset of 26 New Mexico recombinant inbred lines of pepper (Capsicum annuum). Within the P. capsici populations from Brazil, eight new physiological races for the root rot disease syndrome were identified. A total of nine isolates were pathogenic only on the susceptible control, ‘Camelot’. The ability to identify physiological races of P. capsici occurring in Brazil allows for a better understanding about race-specific resistance leading to improved approaches in breeding for durable resistant cultivars.

scholarly journals Identification of Novel Physiological Races of Phytophthora capsici Causing Foliar Blight Using the New Mexico Recombinant Inbred Pepper Lines Set as a Host Differential

2011 ◽  
Vol 136 (3) ◽  
pp. 205-210 ◽  
Author(s):  
Ariadna Monroy-Barbosa ◽  
Paul W. Bosland
Keyword(s):  
New Mexico ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Phytophthora Capsici ◽  
The United States ◽  
Inbred Lines ◽  
Physiological Races ◽  
Foliar Blight ◽  
Physiological Race ◽  
Geographical Locations

Phytophthora foliar blight caused by Phytophthora capsici is a serious limitation to pepper (Capsicum annuum) production worldwide. Knowledge of the physiological race composition of isolates causing phytophthora foliar blight is necessary for success in breeding for disease resistance. The New Mexico recombinant inbred lines (NMRILs) effectively differentiated isolates from different geographical locations (i.e., Turkey, The Netherlands, Argentina, and two states in the United States) into 12 physiological races of P. capsici causing phytophthora foliar blight. This research demonstrates the use of the NMRILs to identify P. capsici foliar blight races and the importance of identifying the physiological races occurring in specific regions where a C. annuum cultivar will be grown. Knowing the physiological race(s) in a region will provide valuable information to formulate breeding strategies to deploy durable foliar blight resistance.

Using Recombinant Inbred Lines to Monitor Changes in the Race Structure of Phytophthora capsici in Chile Pepper in New Mexico

2015 ◽  
Vol 16 (4) ◽  
pp. 235-240 ◽  
Author(s):  
Li Jiang ◽  
Soum Sanogo ◽  
Paul W. Bosland
Keyword(s):  
New Mexico ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Phytophthora Capsici ◽  
Resistance Index ◽  
Inbred Lines ◽  
Resistance Testing ◽  
Chile Pepper ◽  
New Races ◽  
Pepper Breeding

Since 2008, 11 races of Phytophthora capsici have been identified in New Mexico based on their reactions to a set of host differentials, the New Mexico Recombinant Inbred Lines (NMRILs) developed by the Chile Pepper Breeding Program at New Mexico State University. The objective of this study was to appraise the current race structure of 13 isolates of P. capsici newly collected from chile pepper fields in New Mexico. Of the 13 isolates, 12 were identified as new races. Race 25 (isolate PWB175) was the most virulent isolate, and showed virulence that was higher than that of race 1 (PWB24), which is widely used as a highly virulent race for resistance testing of chile peppers in the United States, and was considered as a potential risk for chile pepper production in New Mexico. Of the 17 NMRILs tested, only 7 were needed for the identification of the 12 new races. The NMRILs are genetically stable including their disease reactions, and hence, are invaluable as P. capsici race differentials. A resistance index that ranges from 0 to 1 with 1 being resistant to all isolates was developed in this study. Among all the NMRILs evaluated, one NMRIL (NMRIL-R) had a low resistance index of 0.17, demonstrating its susceptibility to most of the isolates, while other NMRILs (A, B, F, O, and Z) showed medium to high levels of resistance to the P. capsici evaluated with the range of resistance index from 0.75 to 0.89. The NMRILs with a high resistance index can be a good source of resistance for chile pepper breeding programs. The results of this study prove that multiple races of P. capsici can exist in a single production field and plant breeders must be cognizant to breed for more than one race in a given production location. Accepted for publication 3 December 2015. Published 17 December 2015.

Breeding for Pepper yellow mosaic virus resistance and agronomic attributes in recombinant inbred lines of chili pepper (Capsicum baccatum L.) using mixed models

2021 ◽  
Vol 282 ◽  
pp. 110025
Author(s):  
Daniele Viana da Costa ◽  
Claudia Lougon de Almeida Paiva ◽  
Cíntia dos Santos Bento ◽  
Cláudia Pombo Sudré ◽  
Thâmara Figueiredo Menezes Cavalcanti ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Recombinant Inbred ◽  
Mixed Models ◽  
Virus Resistance ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Chili Pepper ◽  
Yellow Mosaic Virus ◽  
Capsicum Baccatum

scholarly journals Solanum habrochaites Accession LA1777 Recombinant Inbred Lines Are Not Resistant to Tomato Yellow Leaf Curl Virus or Tomato Mottle Virus

HortScience ◽  
2007 ◽  
Vol 42 (5) ◽  
pp. 1149-1152 ◽  
Author(s):  
Aliya Momotaz ◽  
John W. Scott ◽  
David J. Schuster
Keyword(s):  
Disease Severity ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Tomato Yellow Leaf ◽  
Mottle Virus ◽  
Yellow Leaf ◽  
Ril Population ◽  
Leaf Curl Virus ◽  
Leaf Curl

Cultivated tomato (Solanum lycopersicum L.) accessions have been susceptible to the whitefly-transmitted begomoviruses Tomato yellow leaf curl virus (TYLCV) and Tomato mottle virus (ToMoV) that can cause serious crop damage. S. habrochaites accession LA1777 has been reported to be resistant to TYLCV. To locate putative virus resistance genes, 89 recombinant inbred lines (RILs) previously developed from LA1777 in a tomato background, LA1777 and the susceptible RIL parent E6203, were screened against the begomoviruses TYLCV and ToMoV. An initial study showed 18 RILs had less disease severity to TYLCV or ToMoV. Eight RILs had S. habrochaites alleles at TG27 (restriction fragment length polymorphism marker) on chromosome 1, three RILs had S. habrochaites alleles at TG202 on chromosome 7, and one RIL had S. habrochaites alleles at both marker loci. The RILs with these regions were intercrossed in 10 different cross combinations and F2 seeds were then obtained. The F2 progenies were inoculated separately with both viruses and then evaluated in the field. The F2 plants with less disease severity were selected, but most did not have the markers from the hypothetical resistance regions. The F3 progenies were then inoculated and rated for disease severity to both viruses. None of the F3s demonstrated any increased level of resistance, even if derived from F2s homozygous for the target regions from both chromosomes. All plants from every cross combination were susceptible for both TYLCV and ToMoV, suggesting that there is no begomovirus resistance in the LA1777 RIL population. Some limitations of capturing all genes in an RIL population derived from an outcrossing accession are discussed.

Confirmation of QTLs controlling Ascochyta fabae resistance in different generations of faba bean (Vicia faba L.)

2009 ◽  
Vol 60 (4) ◽  
pp. 353 ◽  
Author(s):  
R. Díaz-Ruiz ◽  
Z. Satovic ◽  
C. M. Ávila ◽  
C. M. Alfaro ◽  
M. V. Gutierrez ◽  
...  
Keyword(s):  
Vicia Faba ◽  
Disease Severity ◽  
Faba Bean ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Ascochyta Blight ◽  
Inbred Lines ◽  
Phenotypic Variance ◽  
Vicia Faba L ◽  
Ascochyta Fabae

Ascochyta blight, caused by Ascochyta fabae Speg., is a disease of faba bean (Vicia faba L.) of worldwide distribution. In this study we have conducted an experiment on Ascochyta fabae resistance in 165 recombinant inbred lines (RILs) developed by single-seed descent from the cross between resistant and susceptible lines (Vf6 × Vf136) in which A. fabae resistance QTLs (quantitative trait loci) have been previously reported in the original F2 population. Recombinant inbred lines were inoculated under controlled growth chamber conditions and evaluated for disease severity and infection type index. The linkage map was constructed by MAPMAKER V2.0 and the QTL analysis was carried out using QTL Cartographer. Two hundred and seventy-seven markers (238 RAPDs, 4 isozymes, 5 ESTs, 1 SCAR, 6 SSRs, 2 STSs, and 21 intron-spanning markers) mapped into 21 linkage groups covering 2.856.7 cM, with a mean inter-marker distance of 12.72 cM. Composite interval mapping identified two zones of putative QTL action in the RIL population for DSL (disease severity on leaves) and DSS (disease severity on stems) traits. Putative QTLs (Af1 and Af2) were identified on chromosome 3 and chromosome 2, respectively, and jointly explained 24% of the phenotypic variance of DSL and 16% of DSS. With this study we have (1) confirmed the QTLs for ascochyta blight resistance found in F3 families in the derived RILs (F6), (2) re-estimated their position and genetic effects, and (3) assessed the stability of these QTLs in different genetic backgrounds by comparison of the mapping data with a previous QTL study.

Diverse sources of resistance to Spodoptera litura (F.) in groundnut

2019 ◽  
Vol 79 (01S) ◽  
Author(s):  
M. A. Saleem ◽  
G. K. Naidu ◽  
H. L. Nadaf ◽  
P. S. Tippannavar
Keyword(s):  
Recombinant Inbred ◽  
Spodoptera Litura ◽  
Hot Spot ◽  
Recombinant Inbred Lines ◽  
Insect Pest ◽  
Inbred Lines ◽  
Leaf Damage ◽  
Botanical Variety ◽  
Resistant Genotypes ◽  
Botanical Varieties

Spodoptera litura an important insect pest of groundnut causes yield loss up to 71% in India. Though many effective chemicals are available to control Spodoptera, host plant resistance is the most desirable, economic and eco-friendly strategy. In the present study, groundnut mini core (184), recombinant inbred lines (318) and elite genotypes (44) were studied for their reaction to Spodoptera litura under hot spot location at Dharwad. Heritable component of variation existed for resistance to Spodoptera in groundnut mini core, recombinant inbred lines and elite genotypes indicating scope for selection of Spodoptera resistant genotypes. Only 29 (15%) genotypes belonging to hypogaea, fastigiata and hirsuta botanical varieties under mini core set, 15 transgressive segregants belonging to fastigiata botanical variety among 318 recombinant inbred lines and three genotypes belonging to hypogaea and fastigiata botanical varieties under elite genotypes showed resistance to Spodoptera litura with less than 10% leaf damage. Negative correlation existed between resistance to Spodoptera and days to 50 per cent flowering indicating late maturing nature of resistant genotypes. Eight resistant genotypes (ICG 862, ICG 928, ICG 76, ICG 2777, ICG 5016, ICG 12276, ICG 4412 and ICG 9905) under hypogaea botanical variety also had significantly higher pod yield. These diverse genotypes could serve as potential donors for incorporation of Spodoptera resistance in groundnut.

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