scholarly journals Evaluation of Virus Resistant Squash and Interaction with Reflective and Nonreflective Mulches

2000 ◽  
Vol 10 (3) ◽  
pp. 574-580 ◽  
Author(s):  
George E. Boyhan ◽  
James E. Brown ◽  
Cynthia Channel-Butcher ◽  
Virginia K. Perdue
Keyword(s):  
Mosaic Virus ◽  
Cucurbita Pepo ◽  
Zucchini Yellow Mosaic Virus ◽  
Final Evaluation ◽  
Yellow Mosaic Virus ◽  
First Year ◽  
Virus Incidence ◽  
Summer Squash ◽  
Second Year

A 3-year study to evaluate mulch type (reflective and black) and new virus resistant summer squash (Cucurbita pepo L.) varieties was undertaken. In the first year of the study (1996), in Shorter, Ala., under slight virus pressure, silver painted mulch suppressed virus symptoms through the final evaluation 2 months after planting. In addition, virus symptoms were significantly more prevalent on `Dixie' compared to `Supersett', `Tigress', `HMX 5727', `Jaguar', `Destiny III', and `Prelude II'. In the second year (1997), two different experiments were conducted in Savannah, Ga., where there was no virus pressure. In the first experiment at the Savannah location, `Tigress' and `HMX 6704' had significantly higher yields than `Destiny III', `Prelude II', `Puma', `Jaguar', `Meigs', `Dixie', and `Supersett'. In the second Savannah experiment, `Prelude II' and `Destiny III' had significantly higher yields than `Zucchini Elite', `Supersett', `HMX 6704', and `Jaguar'. In 1998 at Shorter, there was no difference in virus incidence based on mulch used. Although there were differences in virus incidence among varieties, the lowest incidence was 70% of plants infected for `Prelude II'. In addition to field evaluations, these varieties were evaluated for resistance to zucchini yellow mosaic virus under greenhouse conditions. Varieties HMX 7710, HMX 6704, Puma, Tigress, Prelude II, Jaguar, and Destiny III were significantly more resistant compared to varieties Zucchini Elite, Meigs, Supersett, and Dixie. In conclusion, reflective mulch was effective only under slight virus pressure.

scholarly journals Evaluation of Selected Cucurbitaceous Vegetables for Resistance to Zucchini yellow mosaic virus

Plant Disease ◽  
2013 ◽  
Vol 97 (10) ◽  
pp. 1316-1321 ◽  
Author(s):  
J. Svoboda ◽  
L. Leisova-Svobodova ◽  
M. Amano
Keyword(s):  
Mosaic Virus ◽  
Cucurbita Pepo ◽  
Citrullus Lanatus ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Virus Protein ◽  
Virus Concentration ◽  
Summer Squash ◽  
Polymerase Chain ◽  
Relative Virus

Zucchini yellow mosaic virus (ZYMV) causes considerable losses of cucurbitaceous vegetables grown nearly all over the world; indeed, the commonly planted cultivars are highly susceptible to ZYMV. In all, 3 cultivars of American and 8 of European summer squash (Cucurbita pepo), and 6 Japanese and 21 European cucumber lines (Cucumis sativus), including both slicing and pickling species, were selected for the evaluation of their resistance to the most virulent Czech strain, ZYMV-H (GenBank accession number DQ144054). Butternut squash (Cucurbita moschata) ‘Menina 15’, Chinese slicing cucumber ‘Taichung Mou Gua-1’ (TMG-1), and watermelon (Citrullus lanatus) accession PI 595203 were included in the experiment, because they were reported to be resistant to ZYMV. The tested plants were mechanically inoculated by ZYMV-H and their resistance was assessed through a comparison of the relative virus protein concentrations and visual symptoms. Butternut squash Menina 15, Chinese slicing cucumber TMG-1, Japanese slicing cucumber breeds ‘G22' and ‘A192-18’, and watermelon PI 595203 were evaluated as immune: the virus concentration in their leaves was zero, as verified by polymerase chain reaction. American summer squash ‘Cougar’ and Japanese slicing cucumber breeds ‘A202-18’, ‘R10’, and ‘S93-18’ were clearly resistant, because the virus multiplied at a low rate in these plants. The remaining tested cultivars were tolerant or susceptible to ZYMV.

scholarly journals Evaluation of genotoxicity of the aqueous extracts from wild Artemisia herba-alba and Jasmina montana against Zucchini yellow mosaic virus (ZYMV) in Cucurbita pepo plants

2018 ◽  
pp. 17-29
Author(s):  
Heba A. Mahfouze ◽  
Sherin A. Mahfouze ◽  
Mahmoud E. S. Ottai
Keyword(s):  
Mosaic Virus ◽  
Enzyme Activities ◽  
Cucurbita Pepo ◽  
Random Amplified Polymorphic Dna ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Systemic Resistance ◽  
Aqueous Extracts ◽  
Protein Patterns ◽  
Sds Page

Squash or cucurbit (Cucurbita pepo) is one of the famous and important vegetable plants in most world countries. The squash is infected by the critical Zucchini yellow mosaic virus (ZYMV) in Egypt. Though pesticides can protect the plants of pest infections, there are no effective compounds that can be applied as virucides. In this work, the effects of aqueous extracts from Jasmina montana and Artemisia herba-alba on ZYMV infection in the squash plants were examined. In addition, SDS-PAGE protein patterns and enzyme activities were evaluated and induction of resistance by plant elicitors against ZYMV was studied The results observed that using of the aqueous extracts from A. herba-alba or J. montana extracts prior to ZYMV inoculation recorded 100% inhibition of virus infection. Also, complete inhibition was obtained by treatment of A. herba-alba extract with the virus inoculum led to destroy the virus particles due to presence of the effective compounds (terpens, phenolics and the essential oils) in the plant extract. SDS-PAGE protein profiles and enzyme activities were studied in treated and untreated plants. Genomic DNA variation was studied using random amplified polymorphic DNA (RAPD) and Inter-simple sequence repeat (ISSR) loci. The alterations in RAPD and ISSR assays of representative squash plants can be applied to comprehend of induced systemic resistance. These inducers stimulated resistance in the squash plants. Therefore, elicitors should be taken into consideration in the breeding programs for ZYMV control.

scholarly journals Field Performance Comparison of Two Transgenic Summer Squash Hybrids to Their Parental Hybrid Line

HortScience ◽  
1995 ◽  
Vol 30 (3) ◽  
pp. 492-493 ◽  
Author(s):  
Juan Pablo Arce-Ochoa ◽  
Frank Dainello ◽  
Leonard M. Pike ◽  
David Drews
Keyword(s):  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Field Performance ◽  
Performance Comparison ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Parental Line ◽  
Summer Squash ◽  
Transgenic Squash ◽  
Marketable Fruit

`Pavo', a commercially grown, virus-susceptible squash (Cucurbita pepo L.) hybrid, and two experimental virus-resistant transgenic squash hybrids, XPH-1719 and XPH-1739, were tested for field performance. The two transgenic squash hybrids possess the desired fruit and plant characteristics of their parental line, `Pavo', plus resistance to zucchini yellow mosaic virus and watermelon mosaic virus 2 (XPH-1719), and resistance to zucchini yellow mosaic virus, watermelon mosaic virus 2, and cucumber mosaic virus (XPH-1739). Percent emergence and days to flowering were similar among the three hybrids. XPH-1719 and XPH-1739 were equally effective in producing a high percentage of quality marketable fruit and yields with 90% and 13,800 kg·ha–1 and 87% and 16,500 kg·ha–1, respectively. XPH-1719 and XPH-1739 demonstrated their outstanding virus resistance over `Pavo' by producing only 3% and 14% symptomatic plants, respectively, compared to 53% for `Pavo'. They also produced the lowest percentage of infected fruit, 0% and 7%, respectively, with `Pavo' at 26%.

scholarly journals Yield and Virus Resistance of Summer Squash Cultivars and Breeding Lines in North Carolina

1998 ◽  
Vol 8 (1) ◽  
pp. 31-39 ◽  
Author(s):  
Jonathan R. Schultheis ◽  
S. Alan Walters
Keyword(s):  
North Carolina ◽  
Mosaic Virus ◽  
Virus Resistance ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Breeding Lines ◽  
Summer Squash

Yellow and zucchini squash (Cucurbita pepo L.) cultigens (breeding lines and cultivars) were evaluated over a 2-year (1995 and 1996) period in North Carolina. Yellow squash cultigens that performed well (based on total marketable yields) were `Destiny III', `Freedom III', `Multipik', XPHT 1815, and `Liberator III' in Fall 1995 and HMX 4716, `Superpik', PSX 391, `Monet', `Dixie', XPH 1780, and `Picasso' in Spring 1996. Some of the yellow squash cultigens evaluated had superior viral resistance: XPHT 1815, XPHT 1817, `Freedom III', `Destiny III', `Freedom II', TW 941121, `Prelude II', and `Liberator III' in Fall 1995 and XPHT 1815, `Liberator III', `Prelude II', and `Destiny III' in Fall 1996; all these cultigens were transgenic. The yellow squash cultigens that performed well (based on total marketable yields) in the Fall 1995 test had transgenic virus resistance (`Destiny III', `Freedom III', XPHT 1815, and `Liberator III') or had the Py gene present in its genetic background (`Multipik'). Based on total marketable yields, the best zucchini cultigens were XPHT 1800, `Tigress', XPHT 1814, `Dividend' (ZS 19), `Elite', and `Noblesse' in Fall 1995; and `Leonardo', `Tigress', `Hurricane', `Elite', and `Noblesse' in Spring 1996. The zucchini cultigens with virus resistance were TW 940966, XPHT 1814, and XPHT 1800 in Fall 1995 and XPHT 1800, XPHT 1776, XPHT 1777, XPHT 1814, and XPHT 1784 in Fall 1996. Even though TW 940966 had a high level of resistance in the Fall 1995 test, it was not as high yielding as some of the more susceptible lines. Viruses detected in the field were papaya ringspot virus (PRSV) and watermelon mosaic virus (WMV) for Fall 1995; while PRSV, zucchini yellow mosaic virus (ZYMV), and WMV were detected for Fall 1996. Summer squash cultigens transgenic for WMV and ZYMV have potential to improve yield, especially during the fall when viruses are more prevalent. Most transgenic cultigens do not possess resistance to PRSV, except XPHT 1815 and XPHT 1817. Papaya ringspot virus was present in the squash tests during the fall of both years. Thus, PRSV resistance must be transferred to the transgenic cultigens before summer squash can be grown during the fall season without the risk of yield loss due to viruses.

scholarly journals Danos na produção da abobrinha de moita causados pelo Papaya ringspot virus type W e Zucchini yellow mosaic virus

2007 ◽  
Vol 33 (2) ◽  
pp. 192-194 ◽  
Author(s):  
Mônica Juliani Zavaglia Pereira ◽  
Angelo Aparecido Barbosa Sussel ◽  
Ricardo Ferreira da Silva ◽  
Odair José Kuhn ◽  
Fernanda Domingues ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Virus Type ◽  
Cucurbita Pepo ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Papaya Ringspot Virus

Este trabalho teve por objetivo avaliar, em condições de casa de vegetação e de campo, os danos causados pelo PRSV-W e ZYMV em abobrinha-de-moita (Cucurbita pepo cv. Caserta). As plantas em casa de vegetação foram inoculadas com os vírus individualmente e em mistura aos 12 e 22 dias após emergência (DAE) e aos 5, 15 e 25 DAE no campo. Em casa de vegetação, as infecções com PRSV-W + ZYMV, PRSV-W e ZYMV, na primeira época de inoculação, ocasionaram reduções de área foliar de 39,6%, 36,8% e 12,1%, respectivamente. As massas fresca e seca também foram significativamente afetadas na primeira época de inoculação. No campo, as plantas com infecções individuais ou mistas dos potyvírus produziram frutos não comerciais em quantidades que variaram de 14 a 861 g/planta, dependendo da idade que foram inoculadas. As plantas tratadas com tampão fosfato aos 5, 15 e 25 DAE produziram em média 573 g, 937 g e 1172 g de frutos comerciais e 282 g, 221 g e 192 g de frutos não comerciais, respectivamente. A redução na massa fresca das plantas foi diretamente relacionada com a época de inoculação, com médias de 60,7% para aquelas inoculadas aos 5 DAE e de 22,7% para aquelas inoculadas aos 15 DAE. Na terceira época de inoculação não houve diferença significativa de massa fresca entre os tratamentos.

Phenotypic and marker-assisted pyramiding of genes for resistance to zucchini yellow mosaic virus in oilseed pumpkin (Cucurbita pepo)

2014 ◽  
Vol 134 (1) ◽  
pp. 121-128 ◽  
Author(s):  
Martin Pachner ◽  
Harry S. Paris ◽  
Johanna Winkler ◽  
Tamas Lelley
Keyword(s):  
Mosaic Virus ◽  
Cucurbita Pepo ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus
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