scholarly journals Inheritance and Linkage Relationships of Melon (Cucumis melo L.) Isozymes

1998 ◽  
Vol 123 (2) ◽  
pp. 264-272 ◽  
Author(s):  
Jack E. Staub ◽  
Vladimir Meglic ◽  
James D. McCreight
Keyword(s):  
Cucumis Melo ◽  
Linkage Studies ◽  
Genomic Information ◽  
Linkage Groups ◽  
Map Construction ◽  
Segregation Ratios ◽  
Isozyme Loci ◽  
Cucumis Melo L ◽  
Linkage Relationships ◽  
Linkage Distance

Nineteen polymorphic and eleven monomorphic isozyme loci were identified in thirteen enzyme systems in a survey of four-hundred melon (Cucumis melo L.) accessions. Segregation of allozymes in F2 and backcross (BC) families for isozyme loci agreed with the expected 1:2:1 and 1:1 segregation ratios (P <0.01). Eleven isozyme loci were linked and were integrated to form a map containing two linkage groups spanning 98 cM with a mean linkage distance of ≈9 cM. Linkage groups (A and B) contain the following loci in the order: A Fdp-2, Pgd, Pgm, Mpi-1, Idh, and Ac, and B Pep-gl, Mdh-2, Mdh-4, Mdh-5, Mdh-6. The remaining eight loci (Acp-1, Acp-4, Ak-4, Fdp-1, Gpi, Mpi-2, Pep-la, and Pep-pap) segregated independently. The isozyme map constructed in this study provides genomic information for future linkage studies with economically important traits and concensus map construction through map merging.

scholarly journals 147 GENETIC DIVERSITY, AND INHERITANCE AND LINKAGE OF ISOZYME LOCI IN MELON (Cucumis melo L.)

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 449f-449 ◽  
Author(s):  
V. V. Meglic ◽  
T. F. Horejsi ◽  
J. E. Staub ◽  
J. D. McCreight
Keyword(s):  
Genetic Diversity ◽  
Cucumis Melo ◽  
Multivariate Analyses ◽  
Mendelian Inheritance ◽  
Linkage Groups ◽  
Plant Introductions ◽  
Enzyme Systems ◽  
Isozyme Loci ◽  
Cucumis Melo L ◽  
Linkage Relationships

The genetic diversity of 400 U.S. melon germplasm plant introductions was assessed using 35 enzyme systems. Polymorphisms were observed at 24 putative loci (Ac, Acp1, Acp4, Ak2, Ak3. Ak4, Fdp1, Fdp2, Fdp4, Gpi, Idh, Mdh2, Mdh4, Mdh5, Mdhb, Mpi1, Mpi2, Pgd1, Pgd2, Pgm, Pep-g1, Pep-1a, Pep-pap, Skdh) representing 17 different enzymes. Sixteen loci demonstrated simple Mendelian inheritance. Multivariate analyses aided in reduction of data using 16 loci and linkage relationships were observed among the plant introductions. Two of 16 loci (Pgd1 and Acp1) segregated independently. Fourteen loci were assigned into three linkage groups (A-C): A Fdp1, Fdp2, Acp4, Skdh; B Mdh2, Mdh4, Mdh5, Mdh6, Pep-g1, Pgm; C Mpi2, Ac, Idh.

scholarly journals Inheritance and Linkage Relationships of Isozymes in Easter Cactus

1998 ◽  
Vol 123 (1) ◽  
pp. 98-103
Author(s):  
Maureen C. O'Leary ◽  
Thomas H. Boyle
Keyword(s):  
Interspecific Hybrids ◽  
Triosephosphate Isomerase ◽  
Recombination Frequency ◽  
Polyacrylamide Gel Electrophoresis ◽  
Interspecific Crosses ◽  
Germplasm Collections ◽  
Segregation Ratios ◽  
Isozyme Loci ◽  
Linkage Relationships ◽  
Aberrant Segregation

Polyacrylamide gel electrophoresis was used to study inheritance and linkage of isozymes in Easter cactus (Hatiora species and interspecific hybrids). Five isozyme systems were analyzed: aspartate aminotransferase (AAT), glucose-6-phosphate isomerase (GPI), malate dehydrogenase (MDH), phosphoglucomutase (PGM), and triosephosphate isomerase (TPI). F1, F2, BC1, and S1 progeny were used for inheritance studies. Six polymorphic loci (Aat-1, Gpi-1, Mdh-1, Pgm-1, Pgm-2, and Tpi-2) were identified. Aat-1 and Pgm-1 were linked (recombination frequency = 26% ± 7%), but the other isozyme loci assorted independently. Aberrant segregation ratios were observed in at least one segregating family for all six isozyme loci. We hypothesize that segregation distortion was due to linkage between isozyme loci and other genes subject to pre- or postzygotic selection. The existence of five additional isozyme loci (Aat-2, Gpi-2, Mdh-2, Mdh-3, and Tpi-1) was inferred from segregation patterns and by comparison of isozyme profiles from phylloclades and pollen. These isozyme loci may prove useful for confirming hybridity in intra- and interspecific crosses, determining parentage of cultivars, and assessing genetic diversity in germplasm collections.

scholarly journals Isozyme Variation in Indian and Chinese Melon (Cucumis melo L.) Germplasm Collections

2004 ◽  
Vol 129 (6) ◽  
pp. 811-818 ◽  
Author(s):  
James D. McCreight ◽  
Jack E. Staub ◽  
Anabel López-Sesé ◽  
Sang-Min Chung
Keyword(s):  
Cluster Analysis ◽  
Cucumis Melo ◽  
Genetic Distances ◽  
Germplasm Collections ◽  
Initial Cluster ◽  
Major Cluster ◽  
Isozyme Loci ◽  
Cucumis Melo L ◽  
Made In ◽  
Group 1

Genetic variation among 378 melon (Cucumis melo L.) germplasm accessions collected in India in 1992 and 26 accessions in China in 1994 was evaluated with 19 isozyme loci. `Top Mark' and `Green Flesh Honeydew', which represented two distinct C. melo ssp. melo L. groups, Cantalupensis and Inodorus, respectively, were used as reference cultivars. Genetic distances among accessions were calculated, and an initial cluster analysis using these distances resulted in 148 groups of varying size, ranging from two to 47 accessions. One accession from each of the 148 groups was chosen at random and used in a second cluster analysis that identified 11 accession groups. Group 1 was unique and consisted of only two C. melo ssp. agrestis (Naudin) Pangalo accessions. Two large branches were detected at cluster node 2. One branch was comprised of three groups of 3, 12, and 34 accessions, while the other branch contained seven groups of 2, 3, 14, 16, and 47 accessions, and the reference cultivars. Of the 148 accessions, 132 were from 41 sites in Rajasthan and Madhya Pradesh, India, which were distributed unequally across the 11 groups. The 14 Chinese accessions originating from seven provinces were also dispersed unequally in the four major cluster groups. `Top Mark' and `Green Flesh Honeydew' were genetically distinct and uniquely clustered in the same group. These results indicate that additional collections of melon germplasm should be made in eastern and southern India.

Construction of a reference linkage map for melon

Genome ◽  
2001 ◽  
Vol 44 (5) ◽  
pp. 836-845 ◽  
Author(s):  
M Oliver ◽  
J Garcia-Mas ◽  
M Cardús ◽  
N Pueyo ◽  
A I López-Sesé ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Genetic Distance ◽  
Linkage Map ◽  
Genetic Map ◽  
Cucumis Melo ◽  
Morphological Trait ◽  
Linkage Groups ◽  
Resistant Gene ◽  
Anchor Points ◽  
Cucumis Melo L

A map of melon (Cucumis melo L.) with 411 markers (234 RFLPs, 94 AFLPs, 47 RAPDs, 29 SSRs, five inter-SSRs, and two isozymes) and one morphological trait (carpel number) was constructed using the F2 progeny of a cross between the Korean accession PI161375 and the Spanish melon type 'Pinyonet Piel de Sapo'. RFLPs were obtained using 212 probes from different genomic and cDNA melon libraries, including 16 Arabidopsis ESTs, 13 Cucumis known genes, and three resistant gene homologues. Most loci (391) mapped to 12 major linkage groups, spanning a total genetic distance of 1197 cM, with an average map interval of 3 cM/marker. The remaining 21 loci (six RAPDs and 15 AFLPs) were not linked. A majority (66%) of the markers were codominant (RFLPs, SSRs, and isozymes), making them easily transferable to other melon crosses. Such markers can be used as a reference, to merge other melon and cucumber maps already constructed. Indeed, some of them (23 SSRs, 14 RFLPs, one isozyme, and one morphological trait) could act as anchor points with other published cucurbit maps.Key words: Cucumis melo, genetic map, molecular markers, RFLPs, SSRs.

scholarly journals Flavonoid-Deficient Mutants in Grass Pea (Lathyrus sativusL.): Genetic Control, Linkage Relationships, and Mapping with Aconitase and S-Nitrosoglutathione Reductase Isozyme Loci

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Dibyendu Talukdar
Keyword(s):  
Genetic Analysis ◽  
Extra Chromosome ◽  
Recessive Inheritance ◽  
Linkage Studies ◽  
Grass Pea ◽  
Flavonoid Content ◽  
Banding Patterns ◽  
Trisomic Analysis ◽  
Isozyme Loci ◽  
Linkage Relationships

Two flavonoid-deficient mutants, designated asfldL-1andfldL-2, were isolated in EMS-mutagenized (0.15%, 10 h) M2progeny of grass pea (Lathyrus sativusL.). Both the mutants contained total leaf flavonoid content only 20% of their mother varieties. Genetic analysis revealed monogenic recessive inheritance of the trait, controlled by two different nonallelic loci. The two mutants differed significantly in banding patterns of leaf aconitase (ACO) and S-nitrosoglutathione reductase (GSNOR) isozymes, possessing unique bands inAco 1, Aco 2,andGsnor 2loci. Isozyme loci inherited monogenically showing codominant expression in F2(1 : 2 : 1) and backcross (1 : 1) segregations. Linkage studies and primary trisomic analysis mappedAco 1andfld 1loci on extra chromosome of trisomic-I andAco 2,fld 2,andGsnor 2on extra chromosome of trisomic-IV in linked associations.

Linkage of morphological and isozyme loci in lentil, Lens culinaris L.

1993 ◽  
Vol 73 (4) ◽  
pp. 917-926 ◽  
Author(s):  
R. E. Vaillancourt ◽  
A. E. Slinkard
Keyword(s):  
Key Words ◽  
Linkage Map ◽  
Lens Culinaris ◽  
Morphological Markers ◽  
Linkage Groups ◽  
Wild Progenitor ◽  
Wide Crosses ◽  
Isozyme Loci ◽  
Linkage Relationships

Six new isozymes and three morphological markers were placed on the lentil (Lens culinaris L.) linkage map. The 17 isozymes and 11 morphological markers that were studied formed four linkage groups (I through IV). These linkage groups represent linkage relationships in the cultivated lentil (L. c. ssp. culinaris) and its wild progenitor (L. c. ssp. orientalis). However, in crosses between L. c. ssp. odemensis and other ssp. of L. culinaris, linkage relationships were slightly different. In these crosses markers of linkage groups III and IV showed disturbed segregation and pseudo-linkage. Although wide crosses contain more variability than narrow crosses in lentil, linkage relationships are more difficult to interpret because of the prevalence of disturbed segregation and pseudo-linkage. Key words: Lentil, Lens culinaris, linkage, isozyme, pseudo-linkage

scholarly journals Resistance to Cucurbit Yellowing Stunting Disorder Virus (CYSDV) in Cucumis melo L.

HortScience ◽  
2000 ◽  
Vol 35 (1) ◽  
pp. 110-113 ◽  
Author(s):  
A.I. López-Sesé ◽  
M.L. Gómez-Guillamón
Keyword(s):  
Wild Species ◽  
Cucumis Melo ◽  
Dominant Allele ◽  
The Family ◽  
Segregation Ratios ◽  
Yellowing Disease ◽  
Related Wild Species ◽  
Cucumis Melo L

Forty-four accessions of Cucumis melo L. and related wild species were tested for reaction to a yellowing disease, incited by the recently identified cucurbit yellowing stunting disorder virus (CYSDV), under natural and controlled-inoculation conditions. The C. melo TGR-1551 accession and one Cucumis metuliferus Naud. accession were asymptomatic. The segregation ratios obtained following controlled inoculations of the family produced by crossing TGR-1551 with the susceptible Spanish cv. Piel de Sapo revealed that the resistance to CYSDV in TGR-1551 is conditioned by a dominant allele at one locus. The name Cucurbit yellow stunting and symbol Cys is proposed for this locus.

Inheritance and Linkage Studies with Five Isozyme Loci in Soybean 1

Crop Science ◽  
1987 ◽  
Vol 27 (5) ◽  
pp. 885-892 ◽  
Author(s):  
Jeffrey Griffin ◽  
Reid G. Palmer
Keyword(s):  
Linkage Studies ◽  
Isozyme Loci
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