Isozyme Variation in Indian and Chinese Melon (Cucumis melo L.) Germplasm Collections

Genetic variation among 378 melon (Cucumis melo L.) germplasm accessions collected in India in 1992 and 26 accessions in China in 1994 was evaluated with 19 isozyme loci. `Top Mark' and `Green Flesh Honeydew', which represented two distinct C. melo ssp. melo L. groups, Cantalupensis and Inodorus, respectively, were used as reference cultivars. Genetic distances among accessions were calculated, and an initial cluster analysis using these distances resulted in 148 groups of varying size, ranging from two to 47 accessions. One accession from each of the 148 groups was chosen at random and used in a second cluster analysis that identified 11 accession groups. Group 1 was unique and consisted of only two C. melo ssp. agrestis (Naudin) Pangalo accessions. Two large branches were detected at cluster node 2. One branch was comprised of three groups of 3, 12, and 34 accessions, while the other branch contained seven groups of 2, 3, 14, 16, and 47 accessions, and the reference cultivars. Of the 148 accessions, 132 were from 41 sites in Rajasthan and Madhya Pradesh, India, which were distributed unequally across the 11 groups. The 14 Chinese accessions originating from seven provinces were also dispersed unequally in the four major cluster groups. `Top Mark' and `Green Flesh Honeydew' were genetically distinct and uniquely clustered in the same group. These results indicate that additional collections of melon germplasm should be made in eastern and southern India.

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Inheritance and Linkage Relationships of Melon (Cucumis melo L.) Isozymes

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.123.2.264 ◽

1998 ◽

Vol 123 (2) ◽

pp. 264-272 ◽

Cited By ~ 6

Author(s):

Jack E. Staub ◽

Vladimir Meglic ◽

James D. McCreight

Keyword(s):

Cucumis Melo ◽

Linkage Studies ◽

Genomic Information ◽

Linkage Groups ◽

Map Construction ◽

Segregation Ratios ◽

Isozyme Loci ◽

Cucumis Melo L ◽

Linkage Relationships ◽

Linkage Distance

Nineteen polymorphic and eleven monomorphic isozyme loci were identified in thirteen enzyme systems in a survey of four-hundred melon (Cucumis melo L.) accessions. Segregation of allozymes in F2 and backcross (BC) families for isozyme loci agreed with the expected 1:2:1 and 1:1 segregation ratios (P <0.01). Eleven isozyme loci were linked and were integrated to form a map containing two linkage groups spanning 98 cM with a mean linkage distance of ≈9 cM. Linkage groups (A and B) contain the following loci in the order: A Fdp-2, Pgd, Pgm, Mpi-1, Idh, and Ac, and B Pep-gl, Mdh-2, Mdh-4, Mdh-5, Mdh-6. The remaining eight loci (Acp-1, Acp-4, Ak-4, Fdp-1, Gpi, Mpi-2, Pep-la, and Pep-pap) segregated independently. The isozyme map constructed in this study provides genomic information for future linkage studies with economically important traits and concensus map construction through map merging.

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DOSES DE POTÁSSIO APLICADAS VIA FERTIRRIGAÇÃO POR GOTEJAMENTO SUPERFICIAL E SUBSUPERFICIAL NO MELOEIRO CULTIVADO EM AMBIENTE PROTEGIDO

Irriga ◽

10.15809/irriga.2005v10n2p184-192 ◽

2005 ◽

Vol 10 (2) ◽

pp. 184-192

Author(s):

Inez Gifone Maia Sales ◽

Thales Vinícius de Araújo Viana ◽

Valdemício Ferreira de Sousa ◽

Benito Moreira de Azevedo ◽

Solerne Caminha Costa

Keyword(s):

Drip Irrigation ◽

Cucumis Melo ◽

Fertilizer Application ◽

Fruit Weight ◽

Potassium Fertilizer ◽

Level Increase ◽

Total Productivity ◽

Cucumis Melo L ◽

Potassium 40 ◽

Made In

DOSES DE POTÁSSIO APLICADAS VIA FERTIRRIGAÇÃO POR GOTEJAMENTO SUPERFICIAL E SUBSUPERFICIAL NO MELOEIROCULTIVADO EM AMBIENTE PROTEGIDO Inêz Gifone Maia Sales; Thales Vinícius de Araújo Viana; Valdemício Ferreira de Sousa; Benito Moreira de Azevedo; Solerne Caminha CostaDepartamento de Engenharia Agrícola, Universidade Federal do Ceará, Fortaleza, CE, [email protected] 1 RESUMO Esta pesquisa foi desenvolvida com o objetivo de estudar os efeitos de três doses de potássio, aplicadas via fertirrigação por gotejamento superficial e subsuperficial, na produtividade do meloeiro (Cucumis melo L.). O trabalho experimental foi conduzido, de abril a agosto de 2002, em um ambiente protegido, em Limoeiro do Norte, Ceará. Utilizaram-se mudas do híbrido de meloeiro Bônus 2, espaçadas de 0,5 x 1,0 m, com tutoramento vertical, em espaldeiras de 2,0m de altura. Os tratamentos resultaram de combinações entre doses de potássio (40; 120 e 200 kg ha-1) e o local de aplicação (superfície do solo ou subsuperficialmente). O delineamento experimental foi em blocos ao acaso, com arranjo fatorial 2 x 3, com 4 repetições. Foram avaliadas quatro variáveis: número e peso médio dos frutos e produtividades total e comercial. A aplicação de adubos potássicos na subsuperfície via irrigação não proporcionou diferenças significativas, assim como as doses de potássio aplicadas. UNITERMOS: Cucumis melo L, estufa, irrigação localizada. SALES, I. G. E . ; VIANA, T. V. DE A.; SOUSA, V. F. DE; AZEVEDO, B. M. DE; COSTA, S. C. POTASSIUM DOSES APPLIED BY FERTIRRIGATION USING SUPERFICIAL AND SUBSUPERFICIAL IRRIGATION IN NET MELON IN GREENHOUSE 2 ABSTRACT The purpose of this research was to study the effects of three doses of potassium application, under drip irrigation in two soil profile layers, on melon productivity (Cucumis melo L.). The experiment was carried out in a kind of chapel-shaped greenhouse, in Limoeiro do Norte, CE, Brazil, from April to August 2002. Bonus 2 hybrid young plants were transplanted, separated every 0.5 x 1.0m and vertically supported on 2.0 m high racks. The treatments were a combination of doses of potassium (40; 120 and 200 kg ha-1) and the following nutrient application depths: 0.0 m (surface) and 0.20 m (subsurface). The experimental design was made in randomized blocks with factorial arrangement 2 x 3 and 4 replications. The evaluated variables were: number of fruits, average fruit weight, total productivity and commercial productivity. The potassium fertilizer application on subsurface by irrigation did not provide significant differences, in comparison to a surface application; the potassium level increase from 40 kg ha-1 to 120 kg ha-1 and 200 kg ha-1 did not cause significant changes in the analyzed variables. Keywords: Cucumis melo L, greenhouse, drip irrigation.

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Genetic Analysis of Cucumber Collections Made in India in 1992

HortScience ◽

10.21273/hortsci.32.3.495c ◽

1997 ◽

Vol 32 (3) ◽

pp. 495C-495

Author(s):

J. Staub ◽

Felix Sequen ◽

J.D. McCreight

Keyword(s):

Genetic Variation ◽

Genetic Analysis ◽

Uttar Pradesh ◽

Madhya Pradesh ◽

Isozyme Loci ◽

National Plant Germplasm System ◽

Group 2 ◽

The U.S ◽

Made In ◽

Group 1

Genetic variation in cucumber (Cucumis sativus L.) accessions from India was assessed by examining variation at 21 polymorphic isozyme loci. Forty-six accessions acquired by the U.S. National Plant Germplasm System (NPGS) before 1972 were compared with 146 accessions collected during a 1992 U.S.–India expedition to the states of Rajasthan, Madhya Pradesh, and Uttar Pradesh, India. Two distinct groups (Group 1 and Group 2) were identified within accessions collected in 1992 (0.025 < P < 0.01). Variation at Ak-2, Fdp-2, Gr, Mdh-2, Mpi-1, Per, Pgm, and Skdh was important in the detection of this difference. Group 1 contained 37 (27 Madhya Pradesh + 10 Uttar Pradesh) accessions and Group 2 contains 102 (84 Rajasthan + 18 Madhya Pradesh) accessions. Seven accessions (5 Madhya Pradesh + 2 Rajasthan) were not associated with either group. Isozymic variation in U.S. NPGS accessions acquired before 1972 differed significantly (P < 0.005) from those collected during 1992. When Indian accessions taken collectively (collected before 1972 and in 1992) were compared with an array of 707 C. sativus U.S. NPGS accessions examined previously, relationships differed between accessions grouped by country or subcontinent.

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147 GENETIC DIVERSITY, AND INHERITANCE AND LINKAGE OF ISOZYME LOCI IN MELON (Cucumis melo L.)

HortScience ◽

10.21273/hortsci.29.5.449f ◽

1994 ◽

Vol 29 (5) ◽

pp. 449f-449 ◽

Cited By ~ 1

Author(s):

V. V. Meglic ◽

T. F. Horejsi ◽

J. E. Staub ◽

J. D. McCreight

Keyword(s):

Genetic Diversity ◽

Cucumis Melo ◽

Multivariate Analyses ◽

Mendelian Inheritance ◽

Linkage Groups ◽

Plant Introductions ◽

Enzyme Systems ◽

Isozyme Loci ◽

Cucumis Melo L ◽

Linkage Relationships

The genetic diversity of 400 U.S. melon germplasm plant introductions was assessed using 35 enzyme systems. Polymorphisms were observed at 24 putative loci (Ac, Acp1, Acp4, Ak2, Ak3. Ak4, Fdp1, Fdp2, Fdp4, Gpi, Idh, Mdh2, Mdh4, Mdh5, Mdhb, Mpi1, Mpi2, Pgd1, Pgd2, Pgm, Pep-g1, Pep-1a, Pep-pap, Skdh) representing 17 different enzymes. Sixteen loci demonstrated simple Mendelian inheritance. Multivariate analyses aided in reduction of data using 16 loci and linkage relationships were observed among the plant introductions. Two of 16 loci (Pgd1 and Acp1) segregated independently. Fourteen loci were assigned into three linkage groups (A-C): A Fdp1, Fdp2, Acp4, Skdh; B Mdh2, Mdh4, Mdh5, Mdh6, Pep-g1, Pgm; C Mpi2, Ac, Idh.

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ESTIMATION OF GENETIC SIMILARITY AMONG CULTIVARS OF THEOBROMA CACAO USING MOLECULAR MARKERS

HortScience ◽

10.21273/hortsci.27.6.660g ◽

1992 ◽

Vol 27 (6) ◽

pp. 660g-661

Author(s):

Antonio Figueira ◽

Jules Janick ◽

Peter Goldsbrough

Keyword(s):

Genetic Similarity ◽

Rapd Markers ◽

Genetic Relationships ◽

Genetic Distances ◽

Conservation Strategies ◽

Center Of Origin ◽

Germplasm Collections ◽

Improvement Programs ◽

Single Cluster ◽

Made In

RAPD markers were used to examine genetic similarity in cacao. DNA from 30 cacao cultivars amplified using 15 arbitrary oligonucleotide primers, produced a total of 112 fragments, of which 88% were polymorphic. A phenogram was developed which illustrates the genetic relationships among the cacao cultivars representing the four major geographic groups of cacao (Criollo, Trinitario, Forastero Lower Amazonian, and Forastero Upper Amazonian). The phenogram indicated a general separation of the four groups into three clusters. Criollos and Trinitarios (supposedly hybrids between Forastero and Criollos types) appeared in a single cluster. Lower Amazonian cultivars (mainly selections made in Bahia, Brazil) appeared in a separate cluster. The third cluster consisted of the Upper Amazonian cultivars, which were originally collected from the region believed to be the center of origin of this crop. This cluster displayed the furthest genetic distance from the others. Crosses between Upper Amazon germplasm and local selections have shown heterosis in clonal crosses, which has been exploited in all genetic improvement programs for cacao. We propose that genetic distances based on RAPD markers can be potentially used as a criterion to select parents capable of producing superior hybrids and populations. Genetic relationships can also be useful to define germplasm collections and conservation strategies. Studies are underway to compare phenograms derived from RAPD markers and ribosomal RNA gene polymorphisms.

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