Linkage of morphological and isozyme loci in lentil, Lens culinaris L.

1993 ◽  
Vol 73 (4) ◽  
pp. 917-926 ◽  
Author(s):  
R. E. Vaillancourt ◽  
A. E. Slinkard
Keyword(s):  
Key Words ◽  
Linkage Map ◽  
Lens Culinaris ◽  
Morphological Markers ◽  
Linkage Groups ◽  
Wild Progenitor ◽  
Wide Crosses ◽  
Isozyme Loci ◽  
Linkage Relationships

Six new isozymes and three morphological markers were placed on the lentil (Lens culinaris L.) linkage map. The 17 isozymes and 11 morphological markers that were studied formed four linkage groups (I through IV). These linkage groups represent linkage relationships in the cultivated lentil (L. c. ssp. culinaris) and its wild progenitor (L. c. ssp. orientalis). However, in crosses between L. c. ssp. odemensis and other ssp. of L. culinaris, linkage relationships were slightly different. In these crosses markers of linkage groups III and IV showed disturbed segregation and pseudo-linkage. Although wide crosses contain more variability than narrow crosses in lentil, linkage relationships are more difficult to interpret because of the prevalence of disturbed segregation and pseudo-linkage. Key words: Lentil, Lens culinaris, linkage, isozyme, pseudo-linkage

A peach linkage map integrating RFLPs, SSRs, RAPDs, and morphological markers

Genome ◽  
2001 ◽  
Vol 44 (5) ◽  
pp. 783-790 ◽  
Author(s):  
Maria Teresa Dettori ◽  
Roberta Quarta ◽  
Ignazio Verde
Keyword(s):  
Molecular Markers ◽  
Comparative Analysis ◽  
Linkage Map ◽  
Morphological Traits ◽  
Prunus Persica ◽  
Average Distance ◽  
Morphological Markers ◽  
Linkage Groups ◽  
Peach Genome ◽  
The Common

A linkage map was obtained using a BC1 progeny (Prunus persica × (P. persica × P. ferganensis)). The map is composed of 109 loci (74 RFLPs, 17 SSRs, 16 RAPDs, and two morphological traits) distributed in 10 linkage groups. Loci, segregating in five different ratios, were integrated in the map with JoinMap 2.0 software. The map covers 521 cM of the peach genome. The average distance between adjacent loci is 4.8 cM. Two monogenic traits, flesh adhesion (F/f) and leaf glands (E/e), were placed on the map. Thirty-two loci in common with a saturated linkage map of Prunus allowed a comparative analysis to be made between the two maps. Homologies were found among the respective linkage groups. No relevant differences were observed in the linear order of the common loci.Key words: peach, linkage map, Prunus persica, Prunus ferganensis, molecular markers.

Linkage of random amplified polymorphic DNA, isozyme and morphological markers in grasspea (Lathyrus sativus)

1999 ◽  
Vol 133 (4) ◽  
pp. 389-395 ◽  
Author(s):  
M. A. CHOWDHURY ◽  
A. E. SLINKARD
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Rapd Markers ◽  
Genetic Linkage Map ◽  
Average Distance ◽  
Random Amplified Polymorphic Dna ◽  
Lathyrus Sativus ◽  
Morphological Markers ◽  
Linkage Studies ◽  
Linkage Groups

We constructed a genetic linkage map of grasspea (Lathyrus sativus L.; 2n = 14) from 100 F2 individuals derived from a cross between PI 426891.1.3 and PI 283564c.3.2. A total of 71 RAPD, three isozyme and one morphological markers segregated in the F2 progeny. A small fraction of markers (12%) deviated significantly from the expected Mendelian ratio (1[ratio ]2[ratio ]1 or 3[ratio ]1). Out of 75 markers, 69 (one morphological, three isozyme and 65 RAPD markers) were assigned to 14 linkage groups comprising 898 cM. The average distance between two adjacent markers was 17·2 cM. The present linkage map will serve as a reference point for further linkage studies in grasspea.

Linkage relationships of 19 allozyme encoding loci within the commercial mushroom genus Pleurotus

Genome ◽  
1988 ◽  
Vol 30 (6) ◽  
pp. 888-895 ◽  
Author(s):  
Bernie May ◽  
Kathrine J. Henley ◽  
Christine G. Fisher ◽  
Daniel J. Royse
Keyword(s):  
Linkage Map ◽  
Interspecific Hybrids ◽  
Single Locus ◽  
Linkage Groups ◽  
Edible Fungi ◽  
Single Spore ◽  
Joint Segregation ◽  
The Individual ◽  
Duplicate Loci ◽  
Linkage Relationships

Single spore derived Pleurotus spp. isolates from four commercial lines (two P. sapidus, one P. florida, and one P. ostreatus) and from two interspecific hybrids (P. sajor-caju × P. sapidus) were analyzed for single locus and joint segregation of 25 allozyme encoding loci. The two alleles at the individual loci departed significantly in their segregation from a 1:1 Mendelian ratio in 26% of the intraspecific and 29% of the interspecific tests. Six linkage groups were identified as follows: Dia-1 ~ Est-5; Tpi ~ Pgd-2 ~ Skdh; (Fum) ~ Pgm-2 ~ Pgd-1 ~ PepLgg-1 ~ Gr-2; Ndh ~ Gr-1; Np ~ PepGl-1 ~ Aat-2 ~ Pgk ~ Mup; and Gr-4 ~ Mdh-1. The duplicate loci coding for GR, PEP-LGG, PGM, and PGD were both not linked to each other and not part of duplicate linkage groups. Six loci were not shown to be linked to any other loci (Lap, Pgm-1, Ha, Gpi, PepPap, and PepLgg-2), although the latter two loci were only tested against four and five loci, respectively. The first linkage map of 19 allozyme encoding loci for the Pleurotus genome is presented.Key words: Pleurotus, allozymes, linkage map, inheritance, edible fungi.

scholarly journals Inheritance and Linkage Relationships of Melon (Cucumis melo L.) Isozymes

1998 ◽  
Vol 123 (2) ◽  
pp. 264-272 ◽  
Author(s):  
Jack E. Staub ◽  
Vladimir Meglic ◽  
James D. McCreight
Keyword(s):  
Cucumis Melo ◽  
Linkage Studies ◽  
Genomic Information ◽  
Linkage Groups ◽  
Map Construction ◽  
Segregation Ratios ◽  
Isozyme Loci ◽  
Cucumis Melo L ◽  
Linkage Relationships ◽  
Linkage Distance

Nineteen polymorphic and eleven monomorphic isozyme loci were identified in thirteen enzyme systems in a survey of four-hundred melon (Cucumis melo L.) accessions. Segregation of allozymes in F2 and backcross (BC) families for isozyme loci agreed with the expected 1:2:1 and 1:1 segregation ratios (P <0.01). Eleven isozyme loci were linked and were integrated to form a map containing two linkage groups spanning 98 cM with a mean linkage distance of ≈9 cM. Linkage groups (A and B) contain the following loci in the order: A Fdp-2, Pgd, Pgm, Mpi-1, Idh, and Ac, and B Pep-gl, Mdh-2, Mdh-4, Mdh-5, Mdh-6. The remaining eight loci (Acp-1, Acp-4, Ak-4, Fdp-1, Gpi, Mpi-2, Pep-la, and Pep-pap) segregated independently. The isozyme map constructed in this study provides genomic information for future linkage studies with economically important traits and concensus map construction through map merging.

A SUMMARY OF LINKAGE RELATIONSHIPS AND A REVISED LINKAGE MAP OF THE CHICKEN

1973 ◽  
Vol 15 (3) ◽  
pp. 553-570 ◽  
Author(s):  
R. J. Etches ◽  
R. O. Hawes
Keyword(s):  
Linkage Map ◽  
Linkage Groups ◽  
Independent Segregation ◽  
Linkage Relationships

The literature pertaining to linkage relationships in the chicken has been reviewed. A new linkage map is proposed consisting of 13 linkage groups. In cases where nomenclature has been duplicated, new symbols are proposed. A tabular summary of linkage tests which have shown independent segregation is included.

scholarly journals A Targeted Capture Linkage Map Anchors the Genome of the Schistosomiasis Vector Snail, Biomphalaria glabrata

2017 ◽  
Vol 7 (7) ◽  
pp. 2353-2361 ◽  
Author(s):  
Jacob A Tennessen ◽  
Stephanie R Bollmann ◽  
Michael S Blouin
Keyword(s):  
Linkage Map ◽  
Chromosomal Rearrangements ◽  
Biomphalaria Glabrata ◽  
Linkage Groups ◽  
Genome Sequences ◽  
Human Parasite ◽  
Causal Genes ◽  
Targeted Capture ◽  
Future Work ◽  
Linkage Relationships

Abstract The aquatic planorbid snail Biomphalaria glabrata is one of the most intensively-studied mollusks due to its role in the transmission of schistosomiasis. Its 916 Mb genome has recently been sequenced and annotated, but it remains poorly assembled. Here, we used targeted capture markers to map over 10,000 B. glabrata scaffolds in a linkage cross of 94 F1 offspring, generating 24 linkage groups (LGs). We added additional scaffolds to these LGs based on linkage disequilibrium (LD) analysis of targeted capture and whole-genome sequences of 96 unrelated snails. Our final linkage map consists of 18,613 scaffolds comprising 515 Mb, representing 56% of the genome and 75% of genic and nonrepetitive regions. There are 18 large (&gt; 10 Mb) LGs, likely representing the expected 18 haploid chromosomes, and &gt; 50% of the genome has been assigned to LGs of at least 17 Mb. Comparisons with other gastropod genomes reveal patterns of synteny and chromosomal rearrangements. Linkage relationships of key immune-relevant genes may help clarify snail–schistosome interactions. By focusing on linkage among genic and nonrepetitive regions, we have generated a useful resource for associating snail phenotypes with causal genes, even in the absence of a complete genome assembly. A similar approach could potentially improve numerous poorly-assembled genomes in other taxa. This map will facilitate future work on this host of a serious human parasite.

A linkage map of hexaploid oat based on grass anchor DNA clones and its relationship to other oat maps

Genome ◽  
2001 ◽  
Vol 44 (2) ◽  
pp. 249-265 ◽  
Author(s):  
V A Portyanko ◽  
D L Hoffman ◽  
M Lee ◽  
J B Holland
Keyword(s):  
Linkage Map ◽  
Fragment Length ◽  
Rflp Markers ◽  
Linkage Groups ◽  
Sequence Tagged Sites ◽  
Length Polymorphisms ◽  
Amplified Fragment Length Polymorphisms ◽  
Incomplete Coverage ◽  
Marker Loci ◽  
Isozyme Loci

A cultivated oat linkage map was developed using a recombinant inbred population of 136 F6:7 lines from the cross 'Ogle' × 'TAM O-301'. A total of 441 marker loci, including 355 restriction fragment length polymorphism (RFLP) markers, 40 amplified fragment length polymorphisms (AFLPs), 22 random amplified polymorphic DNAs (RAPDs), 7 sequence-tagged sites (STSs), 1 simple sequence repeat (SSR), 12 isozyme loci, and 4 discrete morphological traits, was mapped. Fifteen loci remained unlinked, and 426 loci produced 34 linkage groups (with 2–43 loci each) spanning 2049 cM of the oat genome (from 4.2 to 174.0 cM per group). Comparisons with other Avena maps revealed 35 genome regions syntenic between hexaploid maps and 16–34 regions conserved between diploid and hexaploid maps. Those portions of hexaploid oat maps that could be compared were completely conserved. Considerable conservation of diploid genome regions on the hexaploid map also was observed (89–95%); however, at the whole-chromosome level, colinearity was much lower. Comparisons among linkage groups, both within and among Avena mapping populations, revealed several putative homoeologous linkage group sets as well as some linkage groups composed of segments from different homoeologous groups. The relationships between many Avena linkage groups remain uncertain, however, due to incomplete coverage by comparative markers and to complications introduced by genomic duplications and rearrangements.Key words: Avena, linkage map, comparative mapping, homoeology.

scholarly journals (261) A PCR-based Linkage Map of Broccoli [Brassica oleracea(L.) var. italica] and Comparisons with Existing BrassicaMaps

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1090B-1090
Author(s):  
Allan Brown ◽  
Khalid Ibrahim ◽  
Sultana Islam ◽  
Elizabeth Jeffery ◽  
John Juvik
Keyword(s):  
Molecular Markers ◽  
Genetic Analysis ◽  
Linkage Map ◽  
Brassica Oleracea ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Morphological Markers ◽  
Linkage Groups ◽  
Interval Width ◽  
Brassica Oleracea L

A set of 216 PCR-based molecular markers was screened for polymorphisms using two morphologically dissimilar broccoli lines, `VI-158' and `BNC'. Fifty-nine of these markers, representing 69 detected polymorphisms and two morphological markers, were used to construct a genetic linkage map of broccoli [Brassicaoleracea (L.) var. italica] from a population of 162 F2:3 families generated from the cross between these two lines. Ten genetic linkage groups were generated that spanned a distance of 468 cM with an average interval width of 9.4 cm. This map represents the first combined SSR and SRAP map of Brassica oleracea. Comparisons are made to existing maps of Brassicanapus and to inter-specific maps of Brassicaoleracea. To our knowledge this is the first linkage map of broccoli [Brassicaoleracea (L.) var. italica] and should provide a useful tool for the genetic analysis of traits specific to the italica subspecies.

scholarly journals 147 GENETIC DIVERSITY, AND INHERITANCE AND LINKAGE OF ISOZYME LOCI IN MELON (Cucumis melo L.)

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 449f-449 ◽  
Author(s):  
V. V. Meglic ◽  
T. F. Horejsi ◽  
J. E. Staub ◽  
J. D. McCreight
Keyword(s):  
Genetic Diversity ◽  
Cucumis Melo ◽  
Multivariate Analyses ◽  
Mendelian Inheritance ◽  
Linkage Groups ◽  
Plant Introductions ◽  
Enzyme Systems ◽  
Isozyme Loci ◽  
Cucumis Melo L ◽  
Linkage Relationships

The genetic diversity of 400 U.S. melon germplasm plant introductions was assessed using 35 enzyme systems. Polymorphisms were observed at 24 putative loci (Ac, Acp1, Acp4, Ak2, Ak3. Ak4, Fdp1, Fdp2, Fdp4, Gpi, Idh, Mdh2, Mdh4, Mdh5, Mdhb, Mpi1, Mpi2, Pgd1, Pgd2, Pgm, Pep-g1, Pep-1a, Pep-pap, Skdh) representing 17 different enzymes. Sixteen loci demonstrated simple Mendelian inheritance. Multivariate analyses aided in reduction of data using 16 loci and linkage relationships were observed among the plant introductions. Two of 16 loci (Pgd1 and Acp1) segregated independently. Fourteen loci were assigned into three linkage groups (A-C): A Fdp1, Fdp2, Acp4, Skdh; B Mdh2, Mdh4, Mdh5, Mdh6, Pep-g1, Pgm; C Mpi2, Ac, Idh.

scholarly journals Secondary Trisomics and Telotrisomics of Rice: Origin, Characterization, and Use in Determining the Orientation of Chromosome Map

Genetics ◽  
1996 ◽  
Vol 143 (1) ◽  
pp. 517-529
Author(s):  
Kuldeep Singh ◽  
D S Multani ◽  
Gurdev S Khush
Keyword(s):  
Linkage Map ◽  
Large Population ◽  
Marker Genes ◽  
Linkage Groups ◽  
Breeding Behavior ◽  
Specific Chromosome ◽  
Chromosome Map ◽  
Primary Trisomics ◽  
Correct Orientation ◽  
Genetic Segregation

Abstract Secondary trisomics and telotrisomics representing the 12 chromosomes of rice were isolated from the progenies of primary trisomics. A large population of each primary trisomic was grown. Plants showing variation in gross morphology compared to the primary trisomics and disomic sibs were selected and analyzed cytologically at diakinesis and pachytene. Secondary trisomics for both arms of chromosomes 1, 2, 6, 7 and 11 and for one arm of chromosomes 4, 5, 8, 9 and 12 were identified. Telotrisomics for short arm of chromosomes 1, 8, 9 and 10 and for long arms of chromosomes 2, 3 and 5 were isolated. These secondary and telotrisomics were characterized morphologically and for breeding behavior. Secondary trisomics 2n + 1S · 1S, 2n + 1L · 1L, 2n + 2S · 2S, 2n + 2L · 2L, 2n + 6S · 6S, 2n + 6L · 6L and 2n + 7L · 7L are highly sterile, and 2n + 1L · 1L, 2n + 2L · 2L and 2n + 7L · 7L do not set any seed even upon backcrossing. Telotrisomics are fertile and vigorous. Genetic segregation of 43 marker genes was studied in the F2 or backcross progenies. On the basis of segregation data, these genes were delimited to specific chromosome arms. Correct orientation of 10 linkage groups was determined and centromere positions on nine linkage groups were approximated. A revised linkage map of rice is presented.

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