scholarly journals Biofficacy of Entomopathogenic Nematode, Heterorhabditis indica against White Grub, Phyllognathus dionysius Feb. Under Laboratory Condition

2015 ◽  
Vol 4 (12) ◽  
pp. 1278-1282
Keyword(s):  
Laboratory Condition ◽  
Entomopathogenic Nematode ◽  
White Grub ◽  
Heterorhabditis Indica

Virulence of two entomopathogenic nematode species, Steinernema sp. (strain PQ16) and Heterorhabditis indica (strain KT3987), to nymphs of the coffee cicada Dundubia nagarasingna

Nematology ◽  
2019 ◽  
Vol 21 (1) ◽  
pp. 35-44 ◽  
Author(s):  
Chau N. Nguyen ◽  
Anh T. Do ◽  
Phap Q. Trinh ◽  
Phuc K. Hoang
Keyword(s):  
Biological Control ◽  
Entomopathogenic Nematodes ◽  
Entomopathogenic Nematode ◽  
Nematode Species ◽  
Mortality Rates ◽  
Coffee Plantations ◽  
Heterorhabditis Indica ◽  
Inoculation Dose ◽  
Pot Trial

Summary The virulence and efficacy of two species of entomopathogenic nematodes, Steinernema sp. (strain PQ16) and Heterorhabditis indica (strain KT3987), against nymphs of the coffee cicada, Dundubia nagarasingna, was evaluated under laboratory and glasshouse conditions. The highest mortality rates of coffee cicada nymphs caused by these two nematode strains were 93.5 and 100%, respectively, at an inoculation dose of 600 infective juveniles (IJ) nymph−1. The virulence (LC50) was established as 137.5 IJ and 149.1 IJ for strains S-PQ16 and H-KT3987, respectively. The highest IJ yields of these nematode strains were 66 × 103 IJ (for S-PQ16) and 134.4 × 103 IJ (for H-KT3987) at a dose of 500 IJ nymph−1. The efficacies of the two nematode strains to coffee cicadas at treated dose of 60 × 103 IJ pot−1 were 84.4 and 88.9% after 30 days, higher than the efficacies at treated dose of 40 × 103 IJ pot−1. The number of IJ in 250 ml of soil at 10, 20 and 30 days after treatment, increased from 0.38 × 103 to 4.80 × 103 IJ in soil treated with a dose of 40 × 103 IJ and from 0.66 × 103 to 5.02 × 103 IJ in soil treated with a dose of 60 × 103 IJ (for S-PQ16). Similarly, for H-KT3987 the number of IJ increased from 0.43 × 103 to 8.99 × 103 IJ and from 0.62 × 103 to 9.64 × 103 IJ, at the respective doses. Based on results of a pot trial from glasshouse modelling, an IJ application dosage for biological control of coffee cicada nymphs in coffee plantations was proposed.

scholarly journals INFECTIVITY OF Heterorhabditis indica (Rhabditida: Heterorhabditidae) IN ADULTS AND LARVAE OF WHITE GRUB (Coleoptera: Melolonthidae)

2012 ◽  
Vol XVIII (3) ◽  
pp. 383-394 ◽  
Author(s):  
María Guadalupe Sánchez-Saavedra ◽  
◽  
Hipólito Cortez-Madrigal ◽  
David Cristobal-Acevedo
Keyword(s):  
White Grub ◽  
Heterorhabditis Indica

scholarly journals Assessing the Pathogenicity of Two Bacteria Isolated from the Entomopathogenic Nematode Heterorhabditis indica against Galleria mellonella and Some Pest Insects

Insects ◽  
2019 ◽  
Vol 10 (3) ◽  
pp. 83
Author(s):  
Rosalba Salgado-Morales ◽  
Fernando Martínez-Ocampo ◽  
Verónica Obregón-Barboza ◽  
Kathia Vilchis-Martínez ◽  
Alfredo Jiménez-Pérez ◽  
...  
Keyword(s):  
16S Rrna ◽  
Galleria Mellonella ◽  
De Novo ◽  
Entomopathogenic Nematode ◽  
23S Rrna ◽  
Its2 Region ◽  
Rrna Gene ◽  
28S Rrna ◽  
Heterorhabditis Indica ◽  
Insecticide Activity

The entomopathogenic nematodes Heterorhabditis are parasites of insects and are associated with mutualist symbiosis enterobacteria of the genus Photorhabdus; these bacteria are lethal to their host insects. Heterorhabditis indica MOR03 was isolated from sugarcane soil in Morelos state, Mexico. The molecular identification of the nematode was confirmed using sequences of the ITS1-5.8S-ITS2 region and the D2/D3 expansion segment of the 28S rRNA gene. In addition, two bacteria HIM3 and NA04 strains were isolated from the entomopathogenic nematode. The genomes of both bacteria were sequenced and assembled de novo. Phylogenetic analysis was confirmed by concatenated gene sequence datasets as Photorhabdus luminescens HIM3 (16S rRNA, 23S rRNA, dnaN, gyrA, and gyrB genes) and Pseudomonas aeruginosa NA04 (16S rRNA, 23S rRNA and gyrB genes). H. indica MOR03 infects Galleria mellonella, Tenebrio molitor, Heliothis subflexa, and Diatraea magnifactella larvae with LC50 values of 1.4, 23.5, 13.7, and 21.7 IJs/cm2, respectively, at 48 h. These bacteria are pathogenic to various insects and have high injectable insecticide activity at 24 h.

scholarly journals Pathogenicity of Entomopathogenic Nematode on Sugarcane White Grub Lepidiota stigma (Coleoptera: Scarabaeidae)

2018 ◽  
Vol 24 (2) ◽  
pp. 83
Author(s):  
I Gusti Agung Ayu Indrayani ◽  
Subiyakto Subiyakto ◽  
Chaerani Chaerani
Keyword(s):  
Insect Pests ◽  
Entomopathogenic Nematode ◽  
Insect Pest ◽  
Saccharum Officinarum ◽  
Infective Juvenile ◽  
White Grub ◽  
Pathology Laboratory ◽  
Average Percentage ◽  
Host Seeking ◽  
Randomized Design

<p>Sugarcane (Saccharum officinarum) is one of high-value commodities in Indonesia for producing sugar. Sugarcane production recently reduced due to insect pests attacked, mainly white grub Lepidiota stigma (Coleoptera: Scarabaeidae). Utilization of entomopathogenic nematodes (EPN) is one of the alternative control methods for sugarcane white grub. The aim of the present<br />study was to select the higher pathogenicity of EPN isolates for controlling the sugarcane insect pest. The study was conducted in Insect Pathology Laboratory of Indonesia Sweeteners and Fiber Crops Research Institute. Nine isolates of EPN, e.g. DKS-1, AGH-1, DKH-1, DKH-5, NH-1, NH-2, PH-1, PH-2, and PH-4 and one untreated control were tested for their pathogenicity against sugarcane white grub, L. stigma. Each treatment was arranged in Completely Randomized Design with three replications. Every treatment consisted of 30 individuals of the third instar of white grub which treated by 2 × 104 infective juvenile or IJ of EPN<br />isolates. Parameter observed was the mortality of sugarcane white grub, L. stigma. The result showed that all of EPN isolates tested were promising pathogenic against the white grub with about 10 to 80% of the average percentage of mortality. However, DKS-1 and PH-1 showed more pathogenic against L. stigma with about 80–90% and 70–80% of white grub mortality,<br />respectively. The highest enhancement of white grub mortality occurred at 72 hours after treatment and it was showed by DKS-1 and PH-1 isolates which increased the percentage of white grub mortality about 57.1 and 50%, respectively. Obtaining the promising isolates of NPS with different host seeking strategies will potentially increase the effectivity of control against white<br />grub with the result to increase the yield of sugarcane.</p>

Steinernema phyllophagae n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Florida, USA

Nematology ◽  
2011 ◽  
Vol 13 (4) ◽  
pp. 425-442 ◽  
Author(s):  
Eileen Buss ◽  
Khuong Nguyen
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
First Generation ◽  
Entomopathogenic Nematode ◽  
White Grub ◽  
Lateral Field ◽  
Quercus Virginiana ◽  
Live Oak ◽  
Tail Tip ◽  
A New Species

AbstractA new species of entomopathogenic nematode, Steinernema phyllophagae n. sp., was isolated from a white grub (Phyllophaga sp.) in a live oak (Quercus virginiana) nursery. The new species is characterised by morphometrics of the infective Sjuvenile (IJ) with body length 1289 (1133-1395) μm, distance from anterior end to the excretory pore 99 (84-120) μm, tail 89 (77-109) μm, ratio a = 31 (27.6-35.5), H% = 57 (51-60) and E% = 110 (70-139). The pattern of the lateral field of IJ of the new species is 2, 5, 6, 8, 6, 2. The male of the first generation can be recognised by the length and shape of the spicule and gubernaculum, position of the excretory pore, D% = 75 (68-80), and GS% = 71 (61-77). The female can be recognised by the vulva having low epiptygma and by the presence of several papilla-like structures near the tail tip. Steinernema phyllophagae n. sp. differs further from other species of Steinernema by characteristics of ITS and D2D3 regions of rDNA.

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