Steinernema phyllophagae n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Florida, USA

Nematology ◽  
2011 ◽  
Vol 13 (4) ◽  
pp. 425-442 ◽  
Author(s):  
Eileen Buss ◽  
Khuong Nguyen
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
First Generation ◽  
Entomopathogenic Nematode ◽  
White Grub ◽  
Lateral Field ◽  
Quercus Virginiana ◽  
Live Oak ◽  
Tail Tip ◽  
A New Species

AbstractA new species of entomopathogenic nematode, Steinernema phyllophagae n. sp., was isolated from a white grub (Phyllophaga sp.) in a live oak (Quercus virginiana) nursery. The new species is characterised by morphometrics of the infective Sjuvenile (IJ) with body length 1289 (1133-1395) μm, distance from anterior end to the excretory pore 99 (84-120) μm, tail 89 (77-109) μm, ratio a = 31 (27.6-35.5), H% = 57 (51-60) and E% = 110 (70-139). The pattern of the lateral field of IJ of the new species is 2, 5, 6, 8, 6, 2. The male of the first generation can be recognised by the length and shape of the spicule and gubernaculum, position of the excretory pore, D% = 75 (68-80), and GS% = 71 (61-77). The female can be recognised by the vulva having low epiptygma and by the presence of several papilla-like structures near the tail tip. Steinernema phyllophagae n. sp. differs further from other species of Steinernema by characteristics of ITS and D2D3 regions of rDNA.

Steinernema beitlechemi n. sp., a new entomopathogenic nematode (Nematoda: Steinernematidae) from South Africa

Nematology ◽  
2016 ◽  
Vol 18 (4) ◽  
pp. 439-453 ◽  
Author(s):  
Harun Çimen ◽  
Vladimír Půža ◽  
Jiří Nermuť ◽  
Justin Hatting ◽  
Tshima Ramakuwela ◽  
...  
Keyword(s):  
South Africa ◽  
New Species ◽  
First Generation ◽  
Entomopathogenic Nematode ◽  
Phylogenetic Analyses ◽  
Monophyletic Clade ◽  
African Species ◽  
Spicule Length ◽  
Tail Tip ◽  
A New Species

Steinernema beitlechemi n. sp. is a new species of entomopathogenic nematode isolated from soil samples collected in Bethlehem, Free State province, South Africa. The infective juveniles of S. beitlechemi n. sp. are characterised by a body length of 696 (606-768) μm, position of nerve ring of 96 (86-105) μm from the head and lateral fields with six ridges (i.e., seven lines) in mid-body. First-generation males of S. beitlechemi n. sp. can be distinguished by a spicule length of 93 (88-100) μm and 12 pairs of genital papillae. First-generation females have a moderately protruding vulva and a rounded tail tip bearing one spine-like projection. The new species is further characterised by sequences of the ITS and partial 28S regions of the ribosomal DNA. Phylogenetic analyses show S. beitlechemi n. sp. as a member of a strongly supported monophyletic clade with three other African species: S. sacchari, S. cameroonense and S. nyetense.

Steinernema ethiopiense sp. n. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Ethiopia

Nematology ◽  
2012 ◽  
Vol 14 (6) ◽  
pp. 741-757 ◽  
Author(s):  
Tewodros Tamiru ◽  
Tewodros Tamiru ◽  
Lieven Waeyenberge ◽  
Tewodros Tamiru ◽  
Lieven Waeyenberge ◽  
...  
Keyword(s):  
New Species ◽  
Phylogenetic Trees ◽  
First Generation ◽  
Second Generation ◽  
Dna Analysis ◽  
Galleria Mellonella ◽  
Entomopathogenic Nematode ◽  
Its Region ◽  
Lateral Field ◽  
Tail Tip

Three isolates (Dero-1, Dero-8 and Mosisa-1) of a new entomopathogenic nematode, S. ethiopiense sp. n., were isolated by baiting soil samples from the Mendi area, Western Wollega, Ethiopia, with last instar wax moth larvae Galleria mellonella. Infective juveniles of S. ethiopiense sp. n. have a body length of 898 (768-1010) μm, a maximum of eight identical ridges (i.e., nine lines) in the lateral field, excretory pore located at mid-pharynx, hyaline layer occupying approximately half of the tail and c′ = 3.2. First generation males lack a caudal mucron, whereas second generation males possess a short spine-like mucron. The spicules are slightly arcuate, golden-brown in colour and have an ellipsoid or oblong manubrium. First generation females lack a postanal swelling and have a minute protuberance on the tail tip whereas second generation females have a postanal swelling and protruding vulva. Based on the morphology, morphometrics and DNA analysis, the new species belongs to the glaseri group. The closest relative species is the afro-tropical S. karii recorded from Kenya. The BLAST analysis of the ITS region of the rDNA revealed a similarity of 93% with S. karii, supporting the validity of S. ethiopiense sp. n. as a new species. In the phylogenetic trees the new species groups together only with S. karii (bootstrap value of 100%), but is also separated from S. karii by a bootstrap value of 100% or 70%.

scholarly journals Morphological and molecular characterisation of Steinernema hermaphroditum n. sp. (Nematoda: Steinernematidae), an entomopathogenic nematode from Indonesia, and its phylogenetic relationships with other members of the genus

Nematology ◽  
2004 ◽  
Vol 6 (3) ◽  
pp. 401-412 ◽  
Author(s):  
S. Patricia Stock ◽  
Christine T. Griffin ◽  
Rani Chaerani
Keyword(s):  
New Species ◽  
First Generation ◽  
Entomopathogenic Nematode ◽  
Phylogenetic Reconstruction ◽  
Infective Juvenile ◽  
Molecular Evidence ◽  
Lateral Field ◽  
Third Stage ◽  
28S Rdna Sequence ◽  
A New Species

Abstract Steinernema hermaphroditum n. sp., a new entomopathogenic nematode isolated from soil samples in the Moluccan islands, Indonesia, is described. Morphological observations as well as biological evidence (cross-hybridisation studies) indicate the distinctness of S. hermaphroditum n. sp. from other Steinernema spp. This new species is characterised by the presence of hermaphrodites in the first adult generation. Key morphological diagnostic characters include: a digitate tail with a mucro and a glandular spermatheca filled with sperm in the first generation hermaphrodite; the value of D%; the morphology of the male spicules and gubernaculum and the number and arrangement of the genital papillae; the values of D%, E% and the pattern of the lateral field of the third-stage infective juvenile. Additionally, molecular evidence obtained from ITS rDNA RFLP profiles, 28S rDNA sequence analyses, and phylogenetic reconstruction provide further evidence to establish this nematode as a new species.

Steinernema texanum n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Texas, USA

Nematology ◽  
2007 ◽  
Vol 9 (3) ◽  
pp. 379-396 ◽  
Author(s):  
Robin Stuart ◽  
Ugur Gozel ◽  
Khuong Nguyen ◽  
Michael Rogers ◽  
Vanessa Andalo
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
Entomopathogenic Nematode ◽  
Its Region ◽  
Ventral Side ◽  
Infective Juvenile ◽  
Sequence Length ◽  
Field Pattern ◽  
Lateral Field ◽  
Tail Tip

AbstractSteinernema texanum n. sp. is characterised by morphometrics of the infective juvenile with body length = 756 μm, distance from anterior end to the excretory pore = 59 μm, tail = 73 μm, ratio a = 25, H% = 59 and E% = 81. The lateral field pattern of the new species is 2, 7, 2, and is typical for the species. The male of the first generation can be recognised by the spicule and the gubernaculum lengths and shapes, position of the excretory pore, D% = 67 and GS% = 75. The female can be recognised by the vulva with very low epiptygma and two wart-like structures anterior to the tail tip that are always present on the ventral side. Steinernema texanum n. sp. is characterised genetically by the sequence of the ITS region (sequence length = 956 bp, the length of ITS1 = 263 bp, ITS2 = 286 bp, composition of its sequence and by 18 autapomorphies) and by sequence of D2D3 regions (sequence length = 860 bp, its composition and 15 autapomorphies). Steinernema texanum n. sp. is closely related to species in the feltiae-group, which include S. akhursti, S. feltiae, S. hebeiense, S. jollieti, S. kraussei, S. kushidai, S. litorale, S. monticolum, S. oregonense, S. sangi, S. silvaticum and S. weiseri. Isolates of the new species were obtained using the Galleria-baiting technique from soil samples taken near Kingsville, Texas, USA.

Steinernema khoisanae n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from South Africa

Nematology ◽  
2006 ◽  
Vol 8 (2) ◽  
pp. 157-175 ◽  
Author(s):  
Antoinette Malan ◽  
Ugur Gozel ◽  
Khuong Nguyen
Keyword(s):  
South Africa ◽  
New Species ◽  
Excretory Pore ◽  
Phylogenetic Trees ◽  
First Generation ◽  
Entomopathogenic Nematode ◽  
Internal Transcribed Spacers ◽  
Infective Juvenile ◽  
Field Pattern ◽  
Lateral Field

AbstractA new species of entomopathogenic nematode, Steinernema khoisanae n. sp. is described from South Africa. The new species is characterised by morphometrics of the infective juvenile with body length 1076 μm, narrow body diameter of 33 μm, excretory pore 94 μm from anterior end, tail 85 μm long, a = 33, D% = 68, H% = 57, and E% = 111. The lateral field pattern of the new species is 2, 7, 8, 6, 4 and 2. The male of the first generation can be recognised by the spicule and the gubernaculum shape, excretory pore located posteriorly near the end of the pharynx, D% = 88, and SW% = 199. The first generation female can be recognised by the non-protruding vulva and tail bearing a prominent mucron. Steinernema khoisanae n. sp. is characterised genetically by sequences of the internal transcribed spacers and D2/D3 regions of 28S ribosomal DNA, by composition of their sequences and by numerous unique, derived, nucleotide character states. Phylogenetic trees show that S. khoisanae n. sp. and other members of the S. glaseri-group form a monophyletic assemblage.

A new entomopathogenic nematode, Steinernema tielingense n. sp. (Rhabditida: Steinernematidae), from north China

Nematology ◽  
2012 ◽  
Vol 14 (3) ◽  
pp. 321-338 ◽  
Author(s):  
Juan Ma ◽  
Shulong Chen ◽  
Xiuhua Li ◽  
Richou Han ◽  
Hari Bahadur Khatri-Chhetri ◽  
...  
Keyword(s):  
New Species ◽  
Body Length ◽  
Excretory Pore ◽  
First Generation ◽  
North China ◽  
Tail Length ◽  
Internal Transcribed Spacers ◽  
Male Body ◽  
Lateral Field ◽  
A New Species

A new species of Steinernema was isolated from shrub soils in Liaoning province during a survey for entomopathogenic nematodes in north China. This nematode was obtained by the insect-baiting technique using last instar larvae of Galleria mellonella (Lepidoptera: Pyralidae). This new species is described herein as S. tielingense n. sp. It belongs to the ‘feltiae-kraussei-oregonense’ group and is characterised by infective third-stage juvenile (IJ) with a body length of 915 (824-979) μm, distance from head to excretory pore of 69 (64-73) μm, tail length of 81 (74-85) μm, ratio E=88 (85-94)%, lateral field with eight ridges at mid-body, first generation male spicule 88 (79-98) μm and gubernaculum 61 (49-70) μm long, and first generation female with a vulval protrusion and ratio D=41 (32-49)%. The new species distinctly differs from the related species S. kraussei, S. silvaticum, S. oregonense and S. cholashanense in the different number of ridges in the lateral fields and hyaline tail length as % of total tail length of IJ and male body length and distance from head to excretory pore. Cross hybridisation tests showed that these species were reproductively isolated. The sequences analyses of the internal transcribed spacers (ITS) and D2-D3 regions of the ribosomal DNA confirm this to be a new species.

Steinernema lamjungense n. sp. (Rhabditida: Steinernematidae), a new species of entomopathogenic nematode from Lamjung district, Nepal

Nematology ◽  
2011 ◽  
Vol 13 (5) ◽  
pp. 589-605 ◽  
Author(s):  
Hari Bahadur Khatri-Chhetri ◽  
Lieven Waeyenberge ◽  
Sergei Spiridonov ◽  
Hira Kaji Manandhar ◽  
Maurice Moens
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
First Generation ◽  
Second Generation ◽  
Entomopathogenic Nematode ◽  
Soil Samples ◽  
Field Pattern ◽  
Lateral Field ◽  
Spicule Length ◽  
A New Species

Abstract A new species of entomopathogenic nematode, Steinernema lamjungense n. sp., was recovered from soil samples collected from Tarku, Lamjung district of Nepal, during a survey in 2007. The analysis of ITS-rDNA sequence placed S. lamjungense n. sp. in the arenarium-glaseri-karii-longicaudum group. The nematode can be separated from other described species of the group by morphological and morphometric characteristics of different stages and by characterisations and phylogeny of DNA sequences of the D2D3 domain of the LSU or ITS regions of rDNA. For infective juveniles, the new species can be recognised by medium-long body length of 832 (690-950) μm, pharynx length of 127 (110-142) μm, H% = 61 (55-69) and E% = 79 (69-97). The lateral field pattern is 2, 6, 7, 8, 3, 2. The males have well curved, yellowish-brown spicules with a prominent velum. The spicule terminus is blunt with a longitudinal ventral notch. Spicule length is 87 (81-94) μm and gubernaculum length is 57 (50-66) μm in the first generation males. A tail mucron is absent in first but present in second generation males. First generation males have a lateral field with a single ridge. A post-anal swelling is not developed in first generation females but is well developed in second generation females. Three other isolates of the same species were recovered from the neighbourhood of the type locality and yet another isolate was collected from Swarketari, Syangja district.

Steinernema everestense n. sp. (Rhabditida: Steinernematidae), a new species of entomopathogenic nematode from Pakhribas, Dhankuta, Nepal

Nematology ◽  
2011 ◽  
Vol 13 (4) ◽  
pp. 443-462 ◽  
Author(s):  
Hira Kaji Manandhar ◽  
Lieven Waeyenberge ◽  
Maurice Moens ◽  
Hari Bahadur Khatri-Chhetri ◽  
Sergei Spiridonov
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Entomopathogenic Nematode ◽  
Ventral View ◽  
Lateral View ◽  
The Body ◽  
Field Pattern ◽  
Lateral Field ◽  
Tail Tip ◽  
A New Species

AbstractA new species of entomopathogenic nematode, Steinernema everestense n. sp., was recovered from soil samples collected from Pakhribas, Dhankuta district of Nepal, during a survey in 2007. The analysis of ITS-rDNA and D2D3 LSU sequences placed S. everestense n. sp. in the feltiae-kraussei-oregonense group. The nematode can be separated from other described species of Steinernema by morphological and morphometric characteristics and by characterisations and phylogeny inferred from DNA sequences of the D2D3 LSU or ITS regions of rDNA. For infective juveniles, the new species can be recognised by the body length of 775 (705-838) μm, pharynx 119 (110-130) μm long, H% = 61 (47-68) and E% = 78 (60-89). The lateral field pattern is 2, 8, 6, 4, 3. The males have well curved, brownish-yellow spicules with a prominent but short velum, gubernaculum plump, boat-shaped to almost straight in lateral view, cuneus long, needle-shaped, corpus with two wings in ventral view. Spicule and gubernaculum length of the first generation males is 79 (65-87) μm and 50 (39-57) μm, respectively. A tail mucron is present in both male generations. Genital papillae total 23 or 25, comprising 11 or 12 pairs and a single midventral papilla. Specimens with 23 genital papillae have eight precloacal pairs (seven pairs subventral and one pair lateral), two pairs subterminal and one pair subdorsal. The most posterior two pairs are consistently located near the tail tip. The specimens with 25 genital papillae have an extra adcloacal pair of papillae and the anteriormost two pairs are close together. The vulva is slightly protruding, mostly with equal lips, and low epiptygmata. A post-anal swelling is developed in most first and all second generation females.

A new entomopathogenic nematode, Steinernema xinbinense n. sp. (Nematoda: Steinernematidae), from north China

Nematology ◽  
2012 ◽  
Vol 14 (6) ◽  
pp. 723-739 ◽  
Author(s):  
Juan Ma ◽  
Juan Ma ◽  
Shulong Chen ◽  
Juan Ma ◽  
Shulong Chen ◽  
...  
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
First Generation ◽  
Galleria Mellonella ◽  
Entomopathogenic Nematode ◽  
Tail Length ◽  
Northern China ◽  
Morphological Characters ◽  
Liaoning Province ◽  
Lateral Field

During a survey for entomopathogenic nematodes in northern China, a new species of Steinernema was isolated from soil samples collected from Xinbin county, Liaoning province. This nematode was obtained by the insect-baiting technique using last instar larvae of Galleria mellonella. It is described herein as S. xinbinense n. sp. The nematode can be separated from other described species of the group by morphological and morphometric characteristics of the different stages and by characterisation and phylogeny of DNA sequences of the D2D3 domain of the LSU or ITS regions of rDNA. This new species is characterised by the following morphological characters: infective third-stage juvenile with a body length of 694 (635-744) μm, distance from head to excretory pore of 51 (46-53) μm, tail length of 73 (61-81) μm, E = 71 (65-78)%, presence of eight unevenly spaced and developed ridges in middle lateral field (i.e., nine lines). First generation male with well curved, yellowish spicules 56 (49-62) μm long and gubernaculum 35 (30-41) μm long, small mucron mostly present, first generation female with protruding vulva, tail conical with one or two small mucrons and D = 45 (41-50)%. Cross hybridisation tests with S. tielingense, S. kraussei, S. feltiae and S. hebeiense showed that this species was reproductively isolated. The analyses of ITS-rDNA and D2D3 sequence confirm that the studied nematode isolate is a valid new species belonging to the ‘feltiae-kraussei-oregonense’ group.

Bursaphelenchus parayongensis n. sp. (Tylenchina: Aphelenchoididae) found in packaging wood from China

Nematology ◽  
2021 ◽  
pp. 1-13
Author(s):  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Ute Schönfeld ◽  
Xinxin Ma ◽  
Xiaoling Lü
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Excretory Pore ◽  
Posterior Margin ◽  
Posterior Part ◽  
Precloacal Papilla ◽  
Lateral Field ◽  
The Status ◽  
A New Species ◽  
Partial 18S

Summary Bursaphelenchus parayongensis n. sp. was isolated from packaging wood of a consignment from China exported to Germany. The new species is characterised by the lateral field with three lines, 9-13 μm long stylet with small basal swellings, and the excretory pore located near the posterior part of metacorpus. The male spicules are mitten-shaped, the lamina smoothly tapers towards the distal tip. The condylus is broadly rounded, with a small dorsally bent hook, rostrum triangular with sharply pointed tip, and without cucullus. One precloacal papilla, one adcloacal pair and two postcloacal pairs of male papillae present, with the posteriormost pair forming ‘gland papillae’. Bursal flap long, spade-like with posterior margin truncate or irregular. Females have a small vulval flap of 2-3 μm long, and a post-vulval uterine sac extending for 55-77% of the vulva to anus distance. Tail cylindrical, mostly (about 70%) with a robust mucron, very short to less than 3 μm, but occasionally tail terminus bluntly rounded, without mucron. It is similar to species in subgroup 3 of the eggersi group including B. carpini, B. clavicauda, B. cryphali and B. yongensis, but morphologically different. Detailed phylogenetic analysis based on partial 18S, ITS and D2-D3 region of 28S sequences has confirmed the status of this nematode as a new species.

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