Susceptibility of Inbred Lines of Corn to Fusarium Ear Rot
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Ear rot is a globally prevalent class of disease in maize, of which Fusarium ear rot (FER) caused by the fungal pathogen Fusarium verticilloides, is the most commonly reported. In this study, three F2 populations, namely F2-C, F2-D and F2-J, and their corresponding F2:3 families were produced by crossing three highly FER-resistant inbred lines, Cheng351, Dan598, and JiV203 with the same susceptible line, ZW18, for quantitative trait locus (QTL) mapping of FER-resistance. The individual crop plants were inoculated by injecting spore suspension of the pathogen into the kernels of the maize ears. The broad-sense heritability (H2) for FER-resistance was estimated to be as high as 0.76, 0.81, and 0.78 in F2-C, F2-D and F2-J, respectively, indicating that genetic factors played a key role in the phenotypic variation. We detected a total of 20 FER-resistant QTLs in the three F2 populations, among which QTLs derived from the resistant parent Cheng351, Dan598 and JiV203 explained 62.89 to 82.25%, 43.19 to 61.51% and 54.70 to 75.77% of the phenotypic variation, respectively. Among all FER-resistant QTLs detected, qRfer1, qRfer10, and qRfer17 accounted for the phenotypic variation as high as 26.58 to 43.36%, 11.76 to 18.02%, and 12.02 to 21.81%, respectively. Furthermore, QTLs mapped in different F2 populations showed some extent of overlaps indicating potential resistance ‘hotspots’. The FER-resistant QTLs detected in this study can be explored as useful candidates to improve FER-resistance in maize by introducing these QTLs into susceptible maize inbred lines using molecular marker-assisted selection.

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Evaluation of Maize Inbred Lines for Resistance to Fusarium Ear Rot and Fumonisin Accumulation in Grain in Tropical Africa

Plant Disease ◽

10.1094/pdis-91-3-0279 ◽

2007 ◽

Vol 91 (3) ◽

pp. 279-286 ◽

Cited By ~ 42

Author(s):

C. G. Afolabi ◽

P. S. Ojiambo ◽

E. J. A. Ekpo ◽

A. Menkir ◽

R. Bandyopadhyay

Keyword(s):

Disease Severity ◽

Natural Infection ◽

Fusarium Verticillioides ◽

Field Trials ◽

Inbred Lines ◽

Artificial Inoculation ◽

Fusarium Ear Rot ◽

Ear Rot ◽

Maize Inbred Lines ◽

Resistant Lines

Fusarium ear rot and fumonisin contamination is a major problem facing maize growers worldwide, and host resistance is the most effective strategy to control the disease, but resistant genotypes have not been identified. In 2003, a total of 103 maize inbred lines were evaluated for Fusarium ear rot caused by Fusarium verticillioides in field trials in Ikenne and Ibadan, Nigeria. Disease was initiated from natural infection in the Ikenne trial and from artificial inoculation in the Ibadan trial. Ear rot severity ranged from 1.0 to 6.0 in both locations in 2003. Fifty-two inbred lines with disease severity ≤3 (i.e., ≤ 10% visible symptoms on ears) were selected and reevaluated in 2004 for ear rot resistance, incidence of discolored kernels, and fumonisin contamination in grain. At both locations, ear rot severity on the selected lines was significantly (P < 0.0020) higher in 2004 than in 2003. The effects of selected inbred lines on disease severity were highly significant at Ikenne (P = 0.0072) and Ibadan (P < 0.0001) in 2004. Inbred lines did not affect incidence of discolored kernels at both locations and across years except at Ikenne (P = 0.0002) in 2004. Similarly, significant effects of inbred lines on fumonisin concentration were observed only at Ikenne (P = 0.0201) in 2004. However, inbred lines 02C14585, 02C14593, 02C14603, 02C14606, 02C14624, and 02C14683 had consistently low disease severity across years and locations. Fumonisin concentration was significantly correlated with ear rot only at Ikenne (R = 0.42, P < 0.0001). Correlation between fumonisin concentration and incidence of discolored kernels was also significant at Ikenne (R = 0.39, P < 0.0001) and Ibadan (R = 0.35, P = 0.0007). At both locations, no significant inbred × year interaction was observed for fumonisin concentration. Five inbred lines, namely 02C14585, 02C14603, 02C14606, 02C14624, and 02C14683, consistently had the lowest fumonisin concentration in both trials. Two of these inbred lines, 02C14624 and 02C14585, had fumonisin levels <5.0 μg/g across years in trials where disease was initiated from both natural infection and artificial inoculation. These lines that had consistently low disease severity are useful for breeding programs to develop fumonisin resistant lines.

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Multi-environment Evaluation of Maize Inbred Lines for Resistance to Fusarium Ear Rot and Fumonisins

Plant Disease ◽

10.1094/pdis-11-15-1360-re ◽

2016 ◽

Vol 100 (10) ◽

pp. 2134-2144 ◽

Cited By ~ 18

Author(s):

L. J. Rose ◽

M. Mouton ◽

I. Beukes ◽

B. C. Flett ◽

C. van der Vyver ◽

...

Keyword(s):

Fusarium Verticillioides ◽

Inbred Lines ◽

Fungal Colonization ◽

Fusarium Ear Rot ◽

Ear Rot ◽

Sources Of Resistance ◽

Resistance Response ◽

Maize Inbred Lines ◽

Target Dna ◽

Local Breeding

Fusarium verticillioides causes Fusarium ear rot (FER) of maize and produces fumonisins, which affects grain quality. Host-plant resistance can reduce both FER and fumonisins in maize. In this study, 18 maize inbred lines were evaluated for resistance to F. verticillioides and fumonisin accumulation at five localities in South Africa. Additive main effects and multiplicative interaction analyses revealed significant environment × genotype interactions, with inbred lines CML 390, US 2540W, RO 424W, and VO 617y-2 consistently exhibiting low FER severity (≤5.4%), fungal target DNA (≤0.1 ng μl−1), and fumonisin levels (≤5.6 ppm). Genotype main effect and genotype × environment biplots showed that inbred lines CML 390, US 2540W, and RO 424W were most resistant to FER, fungal colonization, and fumonisin accumulation, respectively, while inbred line RO 424W was most stable in its resistance response over environments. These inbred lines also demonstrated broad adaptability by consistently exhibiting resistance to FER, fungal colonization, and fumonisins across localities. The identified lines could serve as valuable sources of resistance against F. verticillioides and its fumonisins in local breeding programs.

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Evaluation of Maize Inbred Lines for Resistance to Aspergillus and Fusarium Ear Rot and Mycotoxin Accumulation

Agronomy Journal ◽

10.2134/agronj2009.0004 ◽

2009 ◽

Vol 101 (5) ◽

pp. 1219-1226 ◽

Cited By ~ 37

Author(s):

W. Brien Henry ◽

W. Paul Williams ◽

Gary L. Windham ◽

Leigh K. Hawkins

Keyword(s):

Inbred Lines ◽

Fusarium Ear Rot ◽

Ear Rot ◽

Maize Inbred Lines

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Maize inbred lines resistance to fusarium ear rot

Cereal Research Communications ◽

10.1556/crc.35.2007.2.32 ◽

2007 ◽

Vol 35 (2) ◽

pp. 293-296

Author(s):

Branimir Šimić ◽

Jasenka Ćosić ◽

Vlatka Rozman ◽

Anita Liska

Keyword(s):

Inbred Lines ◽

Fusarium Ear Rot ◽

Ear Rot ◽

Maize Inbred Lines

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Resistance in Maize Inbred Lines to Fusarium verticillioides and Fumonisin Accumulation in South Africa

Plant Disease ◽

10.1094/pdis-08-11-0695 ◽

2012 ◽

Vol 96 (6) ◽

pp. 881-888 ◽

Cited By ~ 26

Author(s):

I. M. Small ◽

B. C. Flett ◽

W. F. O. Marasas ◽

A. McLeod ◽

M. A. Stander ◽

...

Keyword(s):

South Africa ◽

Fusarium Verticillioides ◽

Field Trials ◽

Inbred Lines ◽

Fusarium Ear Rot ◽

Ear Rot ◽

Maize Breeding ◽

Sources Of Resistance ◽

Breeding Lines ◽

Maize Inbred Lines

Fusarium ear rot of maize, caused by Fusarium verticillioides, is an important disease affecting maize production worldwide. Apart from reducing yield and grain quality, F. verticillioides produces fumonisins which have been associated with mycotoxicoses of animals and humans. Currently, no maize breeding lines are known with resistance to F. verticillioides in South Africa. The objective of this study, therefore, was to evaluate 24 genetically diverse maize inbred lines as potential sources of resistance to Fusarium ear rot and fumonisin accumulation in field trials at Potchefstroom and Vaalharts in South Africa. After artificial silk channel inoculation with F. verticillioides, Fusarium ear rot development was determined at harvest and fumonisins B1, B2, and B3 quantified. A significant inbred line by location effect was observed for Fusarium ear rot severity (P ≤ 0.001), although certain lines proved to be consistently resistant across both locations. The individual inbred lines also differed considerably in fumonisin accumulation between Potchefstroom and Vaalharts, with differentiation between susceptible and potentially resistant inbred lines only being possible at Vaalharts. A greenhouse inoculation trial was then also performed on a subset of potentially resistant and highly susceptible lines. The inbred lines CML 390, CML 444, CML 182, VO 617Y-2, and RO 549 W consistently showed a low Fusarium ear rot (<5%) incidence at both Potchefstroom and Vaalharts and in the greenhouse. Two of these inbred lines, CML 390 and CML 444, accumulated fumonisin levels <5 mg kg–1. These lines could potentially act as sources of resistance for use within a maize breeding program.

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Evaluation of the genetic diversity of maize lines by microsatellite markers

Vietnam Journal of Biotechnology ◽

10.15625/1811-4989/15/2/12349 ◽

2018 ◽

Vol 15 (2) ◽

pp. 327-332

Author(s):

Nguyễn Thị Thu ◽

Ngô Anh Tuấn ◽

Vũ Thị Ngọc ◽

Ngô Thị Thùy Linh ◽

Vương Huy Minh ◽

...

Keyword(s):

Genetic Diversity ◽

Genetic Similarity ◽

Dna Marker ◽

Polymorphic Information Content ◽

Inbred Lines ◽

Similarity Coefficient ◽

Fusarium Ear Rot ◽

Ear Rot ◽

Sources Of Resistance ◽

Cross Combination

Recently, quantitative trait loci (QTLs) have been identified for resistance to ear rot. Techniques to ensure accurate phenotyping of Fusarium ear rot have been developed and validated. DNA marker technologies have matured and become economically feasible for some DNA marker assisted selection programs. Research and breeding efforts aimed at improving resistance to the Fusarium ear rot, identifying sources of resistance and characterizing the inheritance of ear rot. Resistance to Fusarium ear rot is under genetic control, but no complete resistance has been identified in maize. Once suitable sources of resistance have been identified, inheritance of resistance should be considered before selecting a breeding strategy, so evaluation of genetic diversity of maize lines is necessary for cross combination. Genetic diversity analysis of 71 inbred lines by 10 SSR markers detected 10 loci with a total of 42 alleles and average of 4.2 alleles per locus, the number of alleles on a locus varied from 3 to 6. Polymorphic Information Content (PIC) varied from 0.5783 to 0.8088, average of 0.6981. The Umc1196 marker revealed the highest polymorphism, the Umc1249 marker gave the lowest polymorphism. 71 inbred lines were divided into seven main groups. The genetic similarity among 71 lines ranged from 0.333 to 0.976. H3062 line maize had the lowest genetic similarity coefficient (0.333). The lowest genetic similarity coefficient was recorded in H3062 and H84 (0.333). Two lines H161 and KC161 had the highest genetic similarity coefficient (0.976). Almost maize lines had high genetic similarity coefficient showing the same similarity in genetic relationship.

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The Reaction of some Maize Hybrids, Created at ARDS TURDA, to Fusarium spp. Infection

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Agriculture ◽

10.15835/buasvmcn-agr:12658 ◽

2017 ◽

Vol 74 (1) ◽

pp. 47

Author(s):

Laura ȘOPTEREAN ◽

Loredana SUCIU ◽

Ana Maria VĂLEAN ◽

Felicia MUREŞANU ◽

Carmen PUIA

Keyword(s):

Production Capacity ◽

Climatic Conditions ◽

Fusarium Ear Rot ◽

Ear Rot ◽

Artificial Infection ◽

Fusarium Spp ◽

Corn Hybrids ◽

Maize Hybrids ◽

Resistance Reaction

The most important disease of maize in Romania are stalk and ear rot, which caused yield losses in average of 20%. The resistant hibrids represent one of the most efficient solution for reducing the field loses caused by Fusarium spp. on the maize (Nagy et al., 2006). Diseases caused by Fusarium spp. can affect the yield and grain quality of maize because of contamination with numerous mycotoxins produced by these fungi (Czembor et al., 2015). The purpose of this paper was to know more about the reaction of different maize hybrids to Fusarium and the evaluating the effect of ear rot on the yield ability and mycotoxins accumulation. The experiments carried out at ARDS Turda, during four years (2012-2015). The biological material was represented by 8 hybrids, from different maturity groups, tested in two infection conditions with Fusarium spp. (natural and artificial infections). The temperature and rainfalls of the four years of experiments corresponding to the vegetation of maize (april-september) are influenced favourably the pathogenesis of stalk and ear rot caused by Fusarium spp. and a good discrimination of the resistance reaction of genotypes. Fusarium ear rot has significantly affected production capacity and chemical composition of corn hybrids tested. In conditions of artificial infection with Fusarium spp. was a decrease in the content of starch, fat and increased protein content compared with artificially inoculated variants. The quantity of fumonizin B1+B2 has reached to 5630 μg/kg in conditions of artificial infection. There are negative correlations between production capacity and degree of attack of fusarium ear rot; depending on the reacting genotypes tested increasing disease causes production decrease. The response of maize hybrids to Fusarium infection is influenced by infection and climatic conditions. These factors affect production both in terms of quantity and quality and accumulation of mycotoxins.

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