QTL mapping of Fusarium ear rot resistance in maize

Ear rot is a globally prevalent class of disease in maize, of which Fusarium ear rot (FER) caused by the fungal pathogen Fusarium verticilloides, is the most commonly reported. In this study, three F2 populations, namely F2-C, F2-D and F2-J, and their corresponding F2:3 families were produced by crossing three highly FER-resistant inbred lines, Cheng351, Dan598, and JiV203 with the same susceptible line, ZW18, for quantitative trait locus (QTL) mapping of FER-resistance. The individual crop plants were inoculated by injecting spore suspension of the pathogen into the kernels of the maize ears. The broad-sense heritability (H2) for FER-resistance was estimated to be as high as 0.76, 0.81, and 0.78 in F2-C, F2-D and F2-J, respectively, indicating that genetic factors played a key role in the phenotypic variation. We detected a total of 20 FER-resistant QTLs in the three F2 populations, among which QTLs derived from the resistant parent Cheng351, Dan598 and JiV203 explained 62.89 to 82.25%, 43.19 to 61.51% and 54.70 to 75.77% of the phenotypic variation, respectively. Among all FER-resistant QTLs detected, qRfer1, qRfer10, and qRfer17 accounted for the phenotypic variation as high as 26.58 to 43.36%, 11.76 to 18.02%, and 12.02 to 21.81%, respectively. Furthermore, QTLs mapped in different F2 populations showed some extent of overlaps indicating potential resistance ‘hotspots’. The FER-resistant QTLs detected in this study can be explored as useful candidates to improve FER-resistance in maize by introducing these QTLs into susceptible maize inbred lines using molecular marker-assisted selection.

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Susceptibility of Inbred Lines of Corn to Fusarium Ear Rot 1

Agronomy Journal ◽

10.2134/agronj1949.00021962004100080003x ◽

1949 ◽

Vol 41 (8) ◽

pp. 347-348 ◽

Cited By ~ 5

Author(s):

Francis L. Smith ◽

Catharine Becker Madsen

Keyword(s):

Inbred Lines ◽

Fusarium Ear Rot ◽

Ear Rot

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QTL Mapping for Fusarium Ear Rot and Fumonisin Contamination Resistance in Two Maize Populations

Crop Science ◽

10.2135/cropsci2005.12-0450 ◽

2006 ◽

Vol 46 (4) ◽

pp. 1734-1743 ◽

Cited By ~ 84

Author(s):

Leilani A. Robertson‐Hoyt ◽

Michael P. Jines ◽

Peter J. Balint‐Kurti ◽

Craig E. Kleinschmidt ◽

Don G. White ◽

...

Keyword(s):

Qtl Mapping ◽

Fusarium Ear Rot ◽

Ear Rot ◽

Maize Populations

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Evaluation of broad spectrum sources of resistance to Fusarium verticillioides and advanced maize breeding lines

World Mycotoxin Journal ◽

10.3920/wmj2010.1206 ◽

2011 ◽

Vol 4 (1) ◽

pp. 43-51 ◽

Cited By ~ 17

Author(s):

A. Lanubile ◽

L. Pasini ◽

M. Lo Pinto ◽

P. Battilani ◽

A. Prandini ◽

...

Keyword(s):

Host Plant ◽

Natural Infection ◽

Fusarium Verticillioides ◽

Inbred Lines ◽

Ear Rot ◽

Preventive Action ◽

Maize Breeding ◽

Sources Of Resistance ◽

Resistant Parent ◽

Breeding Lines

The fungus Fusarium verticillioides is commonly associated with maize production in temperate regions of the world, producing ear rot and grain contamination by fumonisins. Genetic resistance is the best preventive action against fumonisin contamination, although at present no commercial maize hybrids are completely resistant. Several studies of the relationship between Fusarium and other species producing ear rot suggest that these fungal species interact in similar ways with the host plant. Consequently, host plant resistance to one pathogen could be associated with resistance to another. The aim of this study was to introduce sources of resistance to Fusarium spp. into maize inbred lines and to evaluate ear rot severity and fumonisin B1 contamination in advanced breeding lines and hybrids after artificial and natural infection with F. verticillioides. Two inbred lines (CO430 and MP420) with resistance to kernel infection by Fusarium graminearum and Aspergillus flavus, respectively, were crossed and backcrossed to susceptible inbred 1203. The BC1S1 progenies were evaluated for plant morphology and silk date and the selected S1 plants were self-pollinated. The S2 families were evaluated under artificial and natural infection with F. verticillioides. Selected S2 progenies were grown ear-to-row until S5-derived inbreds were developed. The S5 lines were evaluated under artificial and natural infection and used as males to produce single crosses. In both crosses, the mean ear rot of the S5 lines with the lowest ear rot was not significantly different from the resistant parent means. Significant progress was observed in the hybrids regarding ear rot performance: on average, ear rot severity decreased significantly from 23% in check hybrids to 5.3% in CO430-derived hybrids. Our results suggest that inbred lines bred for resistance to A. flavus and F. graminearum ear rot could be used to select advanced breeding lines with increased resistances to F. verticillioides ear rot.

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Mapping QTLs for protein and oil content in soybean by removing the influence of related traits in a four-way recombinant inbred line population

The Journal of Agricultural Science ◽

10.1017/s0021859620000040 ◽

2019 ◽

Vol 157 (9-10) ◽

pp. 659-675 ◽

Cited By ~ 2

Author(s):

Xiyu Li ◽

Hong Xue ◽

Kaixin Zhang ◽

Wenbin Li ◽

Yanlong Fang ◽

...

Keyword(s):

Qtl Mapping ◽

Recombinant Inbred ◽

Oil Content ◽

Molecular Breeding ◽

Recombinant Inbred Lines ◽

Interval Mapping ◽

Inbred Lines ◽

Additive Effects ◽

Linkage Maps ◽

Trait Locus

AbstractProtein content (PC) and oil content (OC) are important breeding traits of soybean [Glycine max (L.) Merr.]. Quantitative trait locus (QTL) mapping for PC and OC is important for molecular breeding in soybean; however, the negative correlation between PC and OC influences the accuracy of QTL mapping. In the current study, a four-way recombinant inbred lines (FW-RILs) population comprising 160 lines derived from the cross (Kenfeng14 × Kenfeng15) × (Heinong48 × Kenfeng19) was planted in eight different environments and PC and OC measured. Conditional and unconditional QTL analyses were carried out by interval mapping (IM) and inclusive complete IM based on linkage maps of 275 simple sequences repeat markers in a FW-RILs population. This analysis revealed 59 unconditional QTLs and 52 conditional QTLs among the FW-RILs. An analysis of additive effects indicated that the effects of 13 protein QTLs were not related to OC, whereas OC affected the expression of 13 and eight QTLs either partially or completely, respectively. Eight QTLs affecting OC were not influenced by PC, whereas six and 26 QTLs were partially and fully affected by PC, respectively. Among the QTLs detected in the current study, two protein QTLs and five oil QTLs had not been previously reported. These findings will facilitate marker-assisted selection and molecular breeding of soybean.

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Diverse Components of Resistance to Fusarium verticillioides Infection and Fumonisin Contamination in Four Maize Recombinant Inbred Families

Toxins ◽

10.3390/toxins11020086 ◽

2019 ◽

Vol 11 (2) ◽

pp. 86 ◽

Cited By ~ 7

Author(s):

Laura Morales ◽

Charles T. Zila ◽

Danilo E. Moreta Mejía ◽

Melissa Montoya Arbelaez ◽

Peter J. Balint-Kurti ◽

...

Keyword(s):

Recombinant Inbred ◽

Food System ◽

Mapping Population ◽

Genetic Relationships ◽

Fusarium Verticillioides ◽

Fusarium Ear Rot ◽

Ear Rot ◽

Ear Morphology ◽

Trait Locus ◽

Accurate Indicator

The fungus Fusarium verticillioides can infect maize ears, causing Fusarium ear rot (FER) and contaminating the grain with fumonisins (FUM), which are harmful to humans and animals. Breeding for resistance to FER and FUM and post-harvest sorting of grain are two strategies for reducing FUM in the food system. Kernel and cob tissues have been previously associated with differential FER and FUM. Four recombinant inbred line families from the maize nested associated mapping population were grown and inoculated with F. verticillioides across four environments, and we evaluated the kernels for external and internal infection severity as well as FUM contamination. We also employed publicly available phenotypes on innate ear morphology to explore genetic relationships between ear architecture and resistance to FER and FUM. The four families revealed wide variation in external symptomatology at the phenotypic level. Kernel bulk density under inoculation was an accurate indicator of FUM levels. Genotypes with lower kernel density—under both inoculated and uninoculated conditions—and larger cobs were more susceptible to infection and FUM contamination. Quantitative trait locus (QTL) intervals could be classified as putatively resistance-specific and putatively shared for ear and resistance traits. Both types of QTL mapped in this study had substantial overlap with previously reported loci for resistance to FER and FUM. Ear morphology may be a component of resistance to F. verticillioides infection and FUM accumulation.

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Evaluation of Maize Inbred Lines for Resistance to Fusarium Ear Rot and Fumonisin Accumulation in Grain in Tropical Africa

Plant Disease ◽

10.1094/pdis-91-3-0279 ◽

2007 ◽

Vol 91 (3) ◽

pp. 279-286 ◽

Cited By ~ 42

Author(s):

C. G. Afolabi ◽

P. S. Ojiambo ◽

E. J. A. Ekpo ◽

A. Menkir ◽

R. Bandyopadhyay

Keyword(s):

Disease Severity ◽

Natural Infection ◽

Fusarium Verticillioides ◽

Field Trials ◽

Inbred Lines ◽

Artificial Inoculation ◽

Fusarium Ear Rot ◽

Ear Rot ◽

Maize Inbred Lines ◽

Resistant Lines

Fusarium ear rot and fumonisin contamination is a major problem facing maize growers worldwide, and host resistance is the most effective strategy to control the disease, but resistant genotypes have not been identified. In 2003, a total of 103 maize inbred lines were evaluated for Fusarium ear rot caused by Fusarium verticillioides in field trials in Ikenne and Ibadan, Nigeria. Disease was initiated from natural infection in the Ikenne trial and from artificial inoculation in the Ibadan trial. Ear rot severity ranged from 1.0 to 6.0 in both locations in 2003. Fifty-two inbred lines with disease severity ≤3 (i.e., ≤ 10% visible symptoms on ears) were selected and reevaluated in 2004 for ear rot resistance, incidence of discolored kernels, and fumonisin contamination in grain. At both locations, ear rot severity on the selected lines was significantly (P < 0.0020) higher in 2004 than in 2003. The effects of selected inbred lines on disease severity were highly significant at Ikenne (P = 0.0072) and Ibadan (P < 0.0001) in 2004. Inbred lines did not affect incidence of discolored kernels at both locations and across years except at Ikenne (P = 0.0002) in 2004. Similarly, significant effects of inbred lines on fumonisin concentration were observed only at Ikenne (P = 0.0201) in 2004. However, inbred lines 02C14585, 02C14593, 02C14603, 02C14606, 02C14624, and 02C14683 had consistently low disease severity across years and locations. Fumonisin concentration was significantly correlated with ear rot only at Ikenne (R = 0.42, P < 0.0001). Correlation between fumonisin concentration and incidence of discolored kernels was also significant at Ikenne (R = 0.39, P < 0.0001) and Ibadan (R = 0.35, P = 0.0007). At both locations, no significant inbred × year interaction was observed for fumonisin concentration. Five inbred lines, namely 02C14585, 02C14603, 02C14606, 02C14624, and 02C14683, consistently had the lowest fumonisin concentration in both trials. Two of these inbred lines, 02C14624 and 02C14585, had fumonisin levels <5.0 μg/g across years in trials where disease was initiated from both natural infection and artificial inoculation. These lines that had consistently low disease severity are useful for breeding programs to develop fumonisin resistant lines.

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Multiple alleles for tuber shape in diploid potato detected by qualitative and quantitative genetic analysis using RFLPs.

Genetics ◽

10.1093/genetics/137.1.303 ◽

1994 ◽

Vol 137 (1) ◽

pp. 303-309 ◽

Cited By ~ 2

Author(s):

H J Van Eck ◽

J M Jacobs ◽

P Stam ◽

J Ton ◽

W J Stiekema ◽

...

Keyword(s):

Qtl Mapping ◽

Quantitative Trait ◽

Genetic Factors ◽

Inbred Strains ◽

Width Ratio ◽

Tuber Shape ◽

Multiple Alleles ◽

Length Polymorphisms ◽

Trait Locus ◽

Diploid Level

Abstract Tuber shape in potato is commonly regarded as displaying continuous variation, yet at the diploid level phenotypes can be discerned visually, having round or long tubers. Inheritance of qualitative tuber shape can be explained by a single locus Ro, round being dominant to long. With restriction fragment length polymorphisms (RFLPs) the Ro locus was mapped on chromosome 10. Tuber shape was also studied as a quantitative trait, using the length/width ratio as trait value. The estimated broad sense heritability was h2 = 0.80. The morphologically mapped Ro locus explained 75% of the genetic variation, indicating the presence of a major quantitative trait locus (QTL) at the Ro locus and minor genetic factors. RFLP alleles linked with Ro alleles were used to divide the progeny into four genotypic classes: RofemaleRomale:Rofemalero:roRomale:roro = 1:1:1:1. The recessive ro allele is identical by descent in both parents. The significantly different effects (P = 0.0157) of the non-identical alleles Rofemale and Romale provided evidence for multiallelism at the Ro locus. Linkage mapping of the Ro locus was compared with QTL mapping. Only those markers which are polymorphic in both parents allow accurate QTL mapping when genetic factors segregate from both parents. This finding applies to QTL mapping in all outbreeders without homozygous inbred strains.

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Identification of a major quantitative trait locus, qGER4.2, conferring resistance to Gibberella ear rot in maize using a Chinese recombination inbred line population

10.21203/rs.2.20975/v1 ◽

2020 ◽

Author(s):

Guangfei Zhou ◽

Shunfa Li ◽

Liang Ma ◽

Fang Wang ◽

Yali Sun ◽

...

Keyword(s):

Inbred Line ◽

Quantitative Trait ◽

Snp Array ◽

Ear Rot ◽

Maize Breeding ◽

Resistant Parent ◽

Gibberella Ear Rot ◽

Prevalent Disease ◽

Stable Qtls ◽

Trait Locus

Abstract Background: Gibberella ear rot (GER), a prevalent disease caused by Fusarium graminearum, can result in yield loss and mycotoxin contamination in maize. Despite that several QTLs related to GER resistance have been reported previously, few of them was identified in Chinese maize inbred lines. In this study, we employed a Chinese recombination inbred line (RIL) population comprising of 204 lines, developed from a cross involving a resistant parent DH4866 and a susceptible line T877. The population was phenotypically evaluated at three field trial locations under artificial inoculation with F. graminearum and genotyped with an Affymetrix microarray CGMB56K SNP Array to detect the quantitative trait loci (QTLs) for resistance to GER. Results: Based on the genetic linkage map constructed using 1,868 bins as markers, a total of 15 QTLs were identified, and both DH4866 and T877 alleles at these QTLs contributed toward resistance. Of these QTLs, five were stably expressed across multiple locations, including three co-localized with previously reported genomic regions. The largest-effect QTL located on chromosome 4, qGER4.2, which accounted for 5.66~17.10% of the phenotypic variation, was detected at all locations. Based on the phenotypic values of the alleles corresponding to the five stable QTLs, a significant correlation (r2 = 0.45) between resistance to GER and the number of resistant alleles was observed. Conclusions: QTL mapping was successfully employed to identify genetic loci conferring resistance to GER by a high-density genetic map constructed from two Chinese maize germplasms. The five stable QTLs identified, especially the large-effect QTL, qGER4.2, will be useful in maize breeding programs aimed at improving GER resistance.

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Linkage Mapping and Genome-Wide Association Study Reveals Conservative QTLs and Candidate Genes for Fusarium Rot Resistance in Maize

10.21203/rs.2.13416/v2 ◽

2020 ◽

Author(s):

Yabin Wu ◽

Zijian Zhou ◽

Chaopei Dong ◽

Jiafa Chen ◽

Junqiang Ding ◽

...

Keyword(s):

Association Study ◽

Candidate Genes ◽

Phenotypic Variation ◽

Linkage Mapping ◽

Genome Wide Association Study ◽

Recombinant Inbred Lines ◽

Inbred Lines ◽

Genome Wide Association ◽

Ear Rot ◽

Genome Wide

Abstract Background: Fusarium ear rot (FER) caused by Fusarium verticillioides is a major disease of maize that reduces grain yield and quality globally. However, there have been few reports of major loci for FER were verified and cloned. Result: To gain a comprehensive understanding of the genetic basis of natural variation in FER resistance, a recombinant inbred lines (RIL) population and one panel of inbred lines were used to map quantitative trait loci (QTL) for resistance. As a result, a total of 10 QTL were identified by linkage mapping under four environments, which were located on six chromosomes and explained 1.0%-7.1% of the phenotypic variation. Epistatic mapping detected four pairs of QTL that showed significant epistasis effects, explaining 2.1%-3.0% of the phenotypic variation. Additionally, 18 single nucleotide polymorphisms (SNPs) were identified across the whole genome by genome-wide association study (GWAS) under five environments. Compared linkage and association mapping revealed five common intervals located on chromosomes 3, 4, and 5 associated with FER resistance, four of which were verified in different near-isogenic lines (NILs) populations. GWAS identified three candidate genes in these consistent intervals, which belonged to the Glutaredoxin protein family , actin-depolymerizing factors (ADFs), and AMP-binding proteins. In addition, two verified FER QTL regions were found consistent with Fusarium cob rot (FCR) and Fusarium seed rot (FSR). Conclusions: These results revealed that multi pathways were involved in FER resistance, which was a complex trait that was controlled by multiple genes with minor effects, and provided important QTL and genes, which could be used in molecular breeding for resistance.

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