A PCR Marker for Growth Habit in Common Wheat Based on Allelic Variation at the
            VRN‐A1
            Gene

Abstract Leaf rust caused by the fungus Puccinia recondita f. sp. tritici is one of the most dangerous diseases of common wheat. Infections caused by fungal pathogens reduce the quantity and quality of yields of many cereal species. The most effective method to limit plant infection is to use cultivars that show rust resistance. Genetically conditioned horizontal-type resistance (racial-nonspecific) is a desirable trait because it is characterized by more stable expression compared to major (R) genes that induce racially specific resistance, often overcome by pathogens. Horizontal resistance is conditioned by the presence of slow rust genes, which include genes Lr34 and Lr46. This study aimed to identify markers linked to both genes in 64 common wheat lines and to develop multiplex PCR reaction conditions that were applied to identify both genes simultaneously. The degree of infestation of the analyzed lines was also assessed in field conditions during the growing season of 2017 and 2018. Simple sequence repeat anchored-polymerase chain reaction (SSR-PCR) marker csLV was identified during analysis in line PHR 4947. The presence of a specific sequence has also been confirmed in multiplex PCR analyses. In addition to gene Lr34, gene Lr46 was identified in this genotype. Lines PHR 4947 and PHR 4819 were characterized by the highest leaf rust resistance in field conditions. During STS-PCR analyses, the marker wmc44 of gene Lr46 was identified in most of the analyzed lines. This marker was not present in the following genotypes: PHR 4670, PHR 4800, PHR 4859, PHR 4907, PHR 4922, PHR 4949, PHR 4957, PHR 4995, and PHR 4997. The presence of a specific sequence has also been confirmed in multiplex PCR analyses. Genotypes carrying the markers of the analyzed gene showed good resistance to leaf rust in field conditions in both 2017 and 2018. Research has demonstrated that marker assisted selection (MAS) and multiplex PCR techniques are excellent tools for selecting genotypes resistant to leaf rust.

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Effect of Allelic Variation and Expression Quantity at Glu-1 Loci on Size Distribution of Glutenin Polymer in Common Wheat

Acta Agronomica Sinica ◽

10.1016/s1875-2780(08)60104-2 ◽

2009 ◽

Vol 35 (9) ◽

pp. 1606-1612

Author(s):

Ping-Ping ZHANG ◽

Hong-Xiang MA ◽

Jin-Bao YAO ◽

Zhong-Hu HE

Keyword(s):

Size Distribution ◽

Common Wheat ◽

Allelic Variation ◽

Glutenin Polymer

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Allelic variations at the HvSNF2 and HvBM5 loci are associated with the heading date and growth habit of barley (Hordeum vulgare L.) under a semi-arid climate

Czech Journal of Genetics and Plant Breeding ◽

10.17221/62/2020-cjgpb ◽

2021 ◽

Author(s):

Salem Marzougui

Keyword(s):

Growth Habit ◽

Allelic Variation ◽

Heading Date ◽

Arid Climate ◽

Hordeum Vulgare L ◽

Pcr Product ◽

Speed Up ◽

Primer Sets ◽

Allelic Variations ◽

Semi Arid

The heading date and growth habit are key factors that regulate the transition from the vegetative to the reproductive stage in barley. In this study, we used PCR based markers to identify the allelic variations in the Vrn-H1 (HvMB5) and Vrn-H2 (HvSNF2) genes and to predict the heading date and growth habit of a collection of Tunisian barley assessed under a semi-arid climate. The allelic variation at HvBM5 revealed two PCR fragments at 830 and 344 bp. Primer sets used to amplify the HvSNF2 gene have resulted in different alleles size of 543, 623, and 700 bp. Different allelic combinations of HVBM5 and HvSNF2 were associated with the heading date and growth habit. The spring and early heading accessions were only characterised by the amplification of the HvSNF2 fragment at 700 bp. All the winter accessions yielded the PCR product HvBM5 at 830 bp, but the variation in the heading date was determined by the HvSNF2 alleles. These DNA markers will be a powerful tool to predict the heading date and growth habit and can be used as markers for the assisted selection to speed up the national breeding programme.

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