Cross Species Amplification of Pennisetum glaucum Microsatellite Markers in Pennisetum purpureum and Genetic Diversity of Napier Grass Accessions

Crop Science ◽  
2012 ◽  
Vol 52 (4) ◽  
pp. 1776-1785 ◽  
Author(s):  
Ana Luisa Sousa Azevedo ◽  
Pricila Palla Costa ◽  
Juarez Campolina Machado ◽  
Marco Antonio Machado ◽  
Antônio Vander Pereira ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Pennisetum Glaucum ◽  
Napier Grass ◽  
Pennisetum Purpureum

scholarly journals Microsatellite markers-based characterisation of elephant grass (Pennisetum purpureum) harvested from selected locations in South-West Nigeria

2020 ◽  
Vol 37 (1) ◽  
pp. 38-45
Author(s):  
O.A. Okukenu ◽  
A.A. Olajide ◽  
P.A. Dele ◽  
M. Wheto ◽  
B.T. Akinyemi ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Dna Extraction ◽  
Morphological Characterization ◽  
Ethanol Solution ◽  
Pennisetum Purpureum ◽  
Elephant Grass ◽  
South West ◽  
Ssr Primers ◽  
Genetic Index

This study was carried out to characterise Pennisetum purpureum harvested from some selected locations in S outh-W estern Nigeria using microsatellite markers. Leaf parts of growing young elephant grass (Pennisetum purpureum) were harvested and immediately preserved in ethanol solution before DNA extraction. Two (2) SSR primers (CTM59 and Xtxp278) were used to assess genetic diversity in Pennisetum purpureum. The result shows that 72% of the molecular variations in the elephant grass exists within the population with 28% among the population; there were no unique characteristics among the Nine (9) populations. Nei genetic index ranged from 0.067 (lowest) observed between Isokan and Odeda populations to 0.158 (highest), between Ifedore and Ikoyi Populations. Morphological characterization showed moderate diversity with two major clusters and one minor cluster. Keyword: Elephant grass; cultivars; locations; markers

scholarly journals Genetic Diversity in Napier Grass (Pennisetum purpureum) Assessed by SSR Markers

2015 ◽  
Vol 7 (7) ◽  
Author(s):  
Geofrey Kawube ◽  
Titus Alicai ◽  
Bramwel Wanjala ◽  
Moses Njahira ◽  
Juma Awalla ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Napier Grass ◽  
Pennisetum Purpureum

The utility of ISSRs for the identification of interspecific hybrids between pearl millet (Pennisetum glaucum [L.] R.Br.) × napier grass (Pennisetum purpureum Schumach)

2021 ◽  
pp. 1-8
Author(s):  
S. S. Jade ◽  
P. S. Takawale ◽  
R. A. Bahulikar
Keyword(s):  
Pearl Millet ◽  
Interspecific Hybrids ◽  
Pennisetum Glaucum ◽  
Early Stage ◽  
Polymorphic Information Content ◽  
Napier Grass ◽  
Separate Group ◽  
Pennisetum Purpureum ◽  
Issr Primer ◽  
Hand Pollination

Abstract Interspecific hybrids between pearl millet (Pennisetum glaucum) and napier grass (Pennisetum purpureum) give rise to perennial fodder crops characterized by high biomass, broad clumps and good palatability. These hybrids are triploid and developed by hand pollination of napier grass pollen on pearl millet panicles. The progeny shows a high percentage of pearl millet genotype due to self-pollination in the female parent. Identification of hybrids at a young stage based on morphological characters is difficult. DNA-based molecular markers have high discriminating power and were used to assess genetic differences between hybrids and their parents. Genetic diversity was studied in 18 pearl millet × napier grass hybrids along with their parents and two released national checks using inter simple sequence repeat (ISSR) markers. Eight ISSR primers gave rise to 125 bands, of which 120 bands were polymorphic. Polymorphic information content and ISSR primer index ranged from 0.40 to 0.49 and 8.88 to 11.14, respectively. The hybrids showed the presence of unique bands, besides those shared with male and female parents. Female (pearl millet) parents formed a separate group in the dendrogram constructed based on ISSR polymorphism. The male (napier grass) parents formed a separate group along with hybrids, indicating a higher similarity of hybrids with the male parents. Principal component analysis and STRUCTURE analyses showed a similar grouping. The close resemblance of hybrids to the male parents confirmed their interspecific origin. The study revealed that ISSR marker analysis could be a quick and reliable method to identify interspecific hybrids at an early stage of growth.

scholarly journals Screening of Pearl Millet [Pennisetum glaucum (L.) R. Br.] Germplam Lines for Drought Tolerance Based on Morpho-physiological Traits and SSR Markers

2021 ◽  
pp. 46-63
Author(s):  
M. L. Choudhary ◽  
M. K. Tripathi ◽  
Sushma Tiwari ◽  
R. K. Pandya ◽  
Neha Gupta ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Drought Tolerance ◽  
Microsatellite Markers ◽  
Pearl Millet ◽  
Ssr Markers ◽  
Pennisetum Glaucum ◽  
Physiological Traits ◽  
Physiological Data ◽  
Major Allele Frequency ◽  
Morphological And Physiological Traits

Aim: The present study was undertaken to analyze genetic diversity among pearl millet genotypes based on drought linked morpho-physiological and microsatellite markers. Study Design: In the present investigation, 96 pearl millet germplasm lines were screened against drought using different morphological and physiological traits along with SSR markers. Place and Duration of the Study: The present study was conducted at College of Agriculture, Gwalior, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior, M.P., India during July 2019 to December, 2020. Methodology: The study was conducted to record different morphological and physiological traits related to drought tolerance and susceptibility. Thirty five microsatellite markers were also used in laboratory to analyze the variability among pearl millet genotypes under study. Results: Pearl millet genotypes were grouped according to their morpho-physiological characteristics. Among 35 SSR markers, twenty-two were successfully amplified across all germplasm lines and seven SSR markers were found to be polymorphic and fifteen markers were monomorphic. All seven polymorphic SSR markers were used consequently for amplification of all the 96 germplasm lines. The range of PIC value was 0.0939 to 0.2980 with the average of 0.2274. The highest PIC value was recorded for the markers Xibmsp26 and Xibmsp29 (0.2980), followed by Xibmsp03 (0.2392), Xibmsp29 (0.2392), Xibmsp06 (0.2289) and Xibmsp07 (0.1948) while the lowest for the marker Xibmsp01 (0.0939). The range of major allele frequency value was 0.7604 to 0.9479 with the average of 0.8363. The range of genetic diversity value was 0.0987 to 0.3644 with the average of 0.2665. Conclusions: According to the morpho-physiological data a total of 22 pearl millet genotypes were found to be grouped distantly from rest of the genotypes. These genotypes had shown their drought tolerance bahaviour however, rests of the genotypes were found to be susceptible against drought.

scholarly journals Genome size and ploidy variations in pearl millet (Pennisetum glaucum) and napier grass (Pennisetum purpureum) genotypes

2019 ◽  
Vol 68 (4) ◽  
pp. 299-305
Author(s):  
David Adedayo Animasaun ◽  
Joseph A. Morakinyo ◽  
Oba T. Mustapha ◽  
Ramar Krishnamurthy
Keyword(s):  
Chromosome Number ◽  
Genome Size ◽  
Pearl Millet ◽  
Pennisetum Glaucum ◽  
Basic Chromosome Number ◽  
Napier Grass ◽  
Pennisetum Purpureum ◽  
Evolutionary Pathway ◽  
Ploidy Levels ◽  
Evolution And Development

Pennisetum glaucum (Pearl millet) and P. purpureum (Napier grass) are economically important members of the genus Pennisetum.  The knowledge of variability in chromosome number, size and genomic content of the species could provide clues on the mechanisms responsible for decrease or increase in genomic content in the evolutionary pathway.  In the present study, twenty nine genotypes consisting of 24 pearl millet and 5 Napier grass were assessed for inter- and intra-variations in chromosome number, ploidy status and genome content.  Conventional cytogenetic was used for chromosome counting and flow cytometry technique for assessing genomic contents of the genotypes.  Pearl millet genotypes were diploid (2n = 14) while Napier grass were mainly polyploid (2n = 28).  Despite differences in the ploidy levels, the basic chromosome number was 7.  The average 2C values was 4.86 pg and 4.58 pg for pearl millet and Napier grass genotypes respectively.  The DNA content per haploid cell was higher among pearl millet genotypes and the genomic size were negatively related to chromosome number and ploidy levels, meanwhile, the GC richness was directly proportional to genomic size of the genotype.  The mean channel values showed that the genotypes were nuclear haploid.  The present study suggests that evolution and development of polyploidy was accompanied with loss in genomic content in Napier grass.  It was also discovered that pearl millet with less number of chromosome, had higher genome size than Napier grass.

scholarly journals Characterization of interphase nuclei from triploid hybrids between Pennisetum purpureum Schumach and Pennisetum glaucum (L.) R. Br.

2010 ◽  
Vol 34 (5) ◽  
pp. 1124-1128
Author(s):  
Cristiane Gouvêa Fajardo ◽  
Lisete Chamma Davide ◽  
Antônio Vander Pereira
Keyword(s):  
Pearl Millet ◽  
Interspecific Hybrid ◽  
Small Proportion ◽  
Pennisetum Glaucum ◽  
Napier Grass ◽  
Pennisetum Purpureum ◽  
Triploid Hybrid ◽  
Interphase Nuclei ◽  
Nucleus Volume

This study evaluated the structure and the volume of interphase nuclei from root meristems of the genotypes of napier grass (Pennisetum purpureum), pearl millet (Pennisetum glaucum) and hybrids resultant of such breeding. In napier grass, nuclei were areticulate. Both pearl millet and the triploid hybrid had semi-reticulate nuclei; also, the hybrid presented a small proportion (6%) of areticulate nuclei. Pearl millet had the highest averages of nuclear dimensions, such as volume, diameter and radius, followed by the interspecific hybrid and napier grass. There was no intraspecific variation for the type of nuclear structure, which indicates this feature is important for cytotaxonomic studies involving the genus Pennisetum. Results demonstrated that chromatin organization in these nuclei was influenced by the number and size of chromosomes, affecting the nucleus volume in the analyzed taxa.

scholarly journals Genetic diversity in Napier grass (Pennisetum purpureum) cultivars: implications for breeding and conservation

AoB Plants ◽  
2013 ◽  
Vol 5 (0) ◽  
pp. plt022-plt022 ◽  
Author(s):  
B. W. Wanjala ◽  
M. Obonyo ◽  
F. N. Wachira ◽  
A. Muchugi ◽  
M. Mulaa ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Napier Grass ◽  
Pennisetum Purpureum

Meiotic behavior in early and recent duplicated hexaploid hybrids of napier grass (Pennisetum purpureum) and pearl millet (Pennisetum glaucum)

Caryologia ◽  
2012 ◽  
Vol 65 (2) ◽  
pp. 114-120 ◽  
Author(s):  
Elisa A.A. Paiva ◽  
Fernanda O. Bustamante ◽  
Sandro Barbosa ◽  
Antonio V. Pereira ◽  
Lisete C. Davide
Keyword(s):  
Pearl Millet ◽  
Pennisetum Glaucum ◽  
Napier Grass ◽  
Pennisetum Purpureum ◽  
Meiotic Behavior
Sign in / Sign up

Export Citation Format

Share Document