SOIL EXTRACTION, ION EXCHANGE RESIN, AND ION EXCHANGE MEMBRANE MEASURES OF SOIL MINERAL NITROGEN DURING INCUBATION OF A TALLGRASS PRAIRIE SOIL

A new water purification ion exchange membrane has been synthesized using an all-aqueous and sustainable process. These thin film membranes exhibit a pin hole free, mesoporous architecture that rapidly removes several classes of pervasive and persistent contaminants from water.

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Effect of the Mass Fraction of Ion-Exchange Resin in a Ralex CM Cation-Exchange Membrane on Demineralization of Phenylalanine Aqueous Salt Solutions by Neutralization Dialysis

Membranes and Membrane Technologies ◽

10.1134/s2517751621020074 ◽

2021 ◽

Vol 3 (2) ◽

pp. 98-106

Author(s):

V. I. Vasil’eva ◽

A. M. Saud ◽

E. M. Akberova

Keyword(s):

Ion Exchange ◽

Cation Exchange ◽

Mass Fraction ◽

Exchange Resin ◽

Ion Exchange Resin ◽

Cation Exchange Membrane ◽

Salt Solutions ◽

Aqueous Salt Solutions ◽

Exchange Membrane

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Penyediaan dan Pencirian Membran Pertukaran Kation dengan Resin untuk Proses Elektrodialisis

Jurnal Teknologi ◽

10.11113/jt.v40.422 ◽

2012 ◽

Author(s):

Mahadevan. M. ◽

Lay Pee Lin ◽

Zainal Abidin M. Y. ◽

Mohamad Roji Sarmidi

Keyword(s):

Ion Exchange ◽

Cation Exchange ◽

Anion Exchange ◽

Exchange Resin ◽

Ion Exchange Resin ◽

Anion Exchange Membrane ◽

Cation Exchange Membrane ◽

Membrane Thickness ◽

Anion Exchange Membranes ◽

Exchange Membrane

Elektrodialisis merupakan suatu proses yang menggunakan perbezaan keupayaan elektrik sebagai daya penggerak yang menyebabkan pergerakan ion-ion dalam sesuatu elektronit. Membran yang digunakan dalam proses ini akan membenarkan sama ada cas-cas positif atau negatif sahaja melaluinya bergantung kepada kumpulan berfungsi yang terikat pada membran. Objektif projek penyelidikan ini adalah untuk menghasilkan membran pertukaran kation yang digunakan dalam proses elektrodialisis. Membran-membran pertukaran kation yang dihasilkan terbahagi kepada lima jenis, dan diberi nama sebagai BERL–30, 40, 50, 60, dan 70. Kelima-lima jenis membran ini berbeza dari segi kandungan resin yang berfungsi sebagai vektor pertukaran cas kation. Di samping penyediaan membran, penyelidikan ini juga meliputi aspek pencirian membran tersebut serta perbandingan dengan membran komersial. Kriteria yang dikaji adalah ketebalan membran, sifat kebolehtelapan membran, kapasiti pertukaran ion, kapasiti kepekatan ion kumpulan berfungsi, morfologi struktur membran, ujian kestabilan kimia dan kandungan air membran. Secara keseluruhannya, didapati peratus kandungan resin yang tinggi boleh meningkatkan kapasiti pertukaran ion, peratus kebolehtelapan membran serta kapasiti kepekatan ion kumpulan berfungsi. Antara membran yang dihasilkan, membran pertukaran kation jenis BERL–70 merupakan membran yang berpotensi dalam penggunaan proses elektrodialisis. Kata kunci: Membran pertukaran kation, polisulfona, resin pertukaran ion, ciri-ciri membran, elekrodialisis The objective of this work is to prepare an anion exchange membrane from polysulfone binder–ion exchange resin, which can be used in electrodialysis process. The cation exchange membranes were prepared by the solution casting method. The prepared anion exchange membranes are different from the conventional cation exchange membranes because its functional group is not derived from chlorosulfonic acid but from the absorption of anion exchange resins. The content of resins in each set of the prepared cation exchange membrane varied and were named as BERL–30, 40, 50, 60, and 70. In addition, the performance and behaviour of the prepared anion exchange membrane were evaluated and compared with the commercial cation exchange membranes. The physico-chemical properties of anion exchange membrane were determined by measuring the membrane thickness, permselectivity, and concentration of ion exchange capacity, chemical stability, water content, and scanning electron microscope (SEM). It was found that the increase in the quantity of resin (%) would increase the capacity of ion exchange, percentage of permselectivity, and capacity concentration of ion exchange group. The experimental results showed that cation exchange membrane has the potential to be used in electrodialysis process. Keywords: Cation exchange membrane, electrodialysis, polysulfone, ion exchange resin, membrane characterisation

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Comparing Traditional Soil Extraction With Ion Exchange Resin Capsules for Determining Sulfur Bioavailability in Semiarid Low-Fertility Soils

Soil Science ◽

10.1097/ss.0000000000000137 ◽

2016 ◽

Vol 181 (1) ◽

pp. 39-43

Author(s):

Rachel L. Buck ◽

Bruce L. Webb ◽

Von D. Jolley ◽

Bruce A. Roundy ◽

Bryan G. Hopkins

Keyword(s):

Ion Exchange ◽

Exchange Resin ◽

Ion Exchange Resin ◽

Low Fertility ◽

Soil Extraction

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OPTIMIZATION OF DNA EXTRACTION METHODS FROM BIOLOGICAL MATERIALS OF HONEY BEES IN A DIFFERENT METAMOTPHOSIS PHASE

Animal Breeding and Genetics ◽

10.31073/abg.52.22 ◽

2016 ◽

Vol 52 ◽

pp. 171-176

Author(s):

M. Palkina ◽

O. Metlitska

Keyword(s):

Ion Exchange ◽

Dna Extraction ◽

Honey Bees ◽

Biological Material ◽

Exchange Resin ◽

Ion Exchange Resin ◽

Extraction Methods ◽

Chelex 100 ◽

Drone Brood ◽

Dna Extraction Methods

The aim of the research – adaptation, optimization and using of existing DNA extraction methods from bees’ biological material with the reagent «Chelex-100" under complex economic conditions of native laboratories, which will optimize labour costs and improve the economic performance of DNA extraction protocol. Materials and methods. In order to conduct the research the samples of honey bees’ biological material: queen pupae exuviae, larvae of drone brood, some adult bees’ bodies (head and thorax) were selected. Bowl and drone brood were obtained from the experimental bee hives of Institute of Apiculture nd. a. P. I. Prokopovich of NAAS. DNA extraction from biosamples of Apis mellifera ssp. was carried out using «Chelex-100®» ion exchange resin in different concentrations and combinations. Before setting tests for determination of quantitative and quality indexes, dilution of DNA samples of the probed object was conducted in ratio 1:40. The degree of contamination with protein and polysaccharide fractions (OD 260/230), quantitative content of DNA (OD 260/280) in the extracted tests were conducted using spectrophotometer of «Biospec – nano» at the terms of sample volume in 2 µl and length of optical way in 0,7 mm [7]. Verification of DNA samples from biological material of bees, isolated by «Chelex-100®», was conducted after cold keeping during 24 hours at 20°C using PСR with primaries to the fragment of gene of quantitative trait locus (QTL) Sting-2 of next structure [8]: 3' – CTC GAC GAG ACG ACC AAC TTG – 5’; 3' – AAC CAG AGT ATC GCG AGT GTT AC – 5’ Program of amplification: 94 °C – 5 minutes – 1 cycle; 94 °C – 1 minute, 57°C – 1 minute, 72 °C – 2 minutes – 30 cycles; elongation after 72°C during 2 minutes – 1 cycle. The division of obtained amplicons was conducted by gel electrophoresis at a low current – 7 µÀ, in 1,5 % agarose gel (Sigma ®) in TAE buffer [7]. The results. At the time of optimization of DNA isolation methods, according to existing methods of foreign experts, it was found optimal volume of ion exchange resin solution was in the proposed concentration: instead of 60 µl of solution used 120 µl of «Chelex-100®», time of incubation was also amended from 30 minutes to 180 minutes [9]. The use of the author's combination of method «Chelex-100®» with lysis enzymes, proteinase K and detergents (1M dithiothreitol), as time of incubation was also amended, which was reduced to 180 minutes instead of the proposed 12 hours [10]. Changes in quality characteristics of obtained DNA in samples after reduction in incubation time were not found. Conclusions. The most economical method of DNA isolation from bees’ biological material is 20% solution of «Chelex-100» ion exchange resin with the duration of the incubation period of 180 minutes. It should also be noted that the best results can be obtained from exuviae, selected immediately after the queen’s exit from bowl, that reduces the likelihood of DNA molecules destruction under the influence of nucleases activation, but not later than 12 hours from release using the technology of isolated obtain of queens.

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