Integrative 'Bricks‑And‑Mortar' Approach for the Fabrication of Poly( <sub>D,L</sub>‑Lactide)/Bioactive Glass Nanocomposite Scaffolds by Freeze‑Casting

AbstractThe citrate-based thermoset elastomer is a promising candidate for bone scaffold material, but the harsh curing condition made it difficult to fabricate porous structure. Recently, poly (1, 8-octanediol-co-Pluronic F127 citrate) (POFC) porous scaffold was creatively fabricated by chitin nanofibrils (ChiNFs) supported emulsion-freeze-casting. Thanks to the supporting role of ChiNFs, the lamellar pore structure formed by directional freeze-drying was maintained during the subsequent thermocuring. Herein, bioactive glass (BG) was introduced into the POFC porous scaffolds to improve bioactivity. It was found the complete replacement of ChiNF particles with BG particles could not form a stable porous structure; however, existing at least 15 wt% ChiNF could ensure the formation of lamellar pore, and the interlamellar distance increased with BG ratios. Thus, the BG granules did not contribute to the formation of pore structure like ChiNFs, however, they surely endowed the scaffolds with enhanced mechanical properties, improved osteogenesis bioactivity, better cytocompatibility as well as quick degradation rate. Reasonably adjusting BG ratios could balance the requirements of porous structure and bioactivity.

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Synthesis, characterization and biocompatibility evaluation of sol–gel derived bioactive glass scaffolds prepared by freeze casting method

Ceramics International ◽

10.1016/j.ceramint.2013.10.115 ◽

2014 ◽

Vol 40 (4) ◽

pp. 5349-5355 ◽

Cited By ~ 24

Author(s):

M. Mozafari ◽

F. Moztarzadeh

Keyword(s):

Bioactive Glass ◽

Sol Gel ◽

Freeze Casting ◽

Casting Method

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Development of 3D Bioactive Nanocomposite Scaffolds Made from Gelatin and Nano Bioactive Glass for Biomedical Applications

Advanced Composites Letters ◽

10.1177/096369351001900204 ◽

2010 ◽

Vol 19 (2) ◽

pp. 096369351001900 ◽

Cited By ~ 32

Author(s):

M. Mozafari ◽

F. Moztarzadeh ◽

M. Rabiee ◽

M. Azami ◽

N. Nezafati ◽

...

Keyword(s):

Tissue Engineering ◽

Bioactive Glass ◽

Biomedical Applications ◽

Cell Attachment ◽

Tissue Engineering Scaffolds ◽

Study Results ◽

Nanocomposite Scaffold ◽

Nanocomposite Scaffolds ◽

First Time

In this research, macroporous, mechanically competent and bioactive nanocomposite scaffolds have been fabricated from cross-linked gelatine (Gel) and nano bioactive glass (nBG) through layer solvent casting combined with freeze-drying and lamination techniques. This study has developed a new composition to produce a new bioactive nanocomposite which is porous with interconnected microstructure, pore sizes are 200-500 μm, porosity are 72%-86%. Also, we have reported formation of chemical bonds between nBG and Gel for the first time. Finally, the in vitro cytocompatability of the scaffolds was assessed using MTT assay and cell attachment study. Results indicated no sign of toxicity and cells found to be attached to the pore walls offered by the scaffolds. These results suggested that the developed nanocomposite scaffold possess the prerequisites for bone tissue engineering scaffolds and it can be used for tissue engineering applications.

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