scholarly journals 1P294 Fiber-optic fluorescent imaging of neural activity in freely-moving mice during sleep and wakefulness(27. Bioimaging,Poster)

2013 ◽  
Vol 53 (supplement1-2) ◽  
pp. S154
Author(s):  
Yasuhiro Kasagi ◽  
Takeshi Kanda ◽  
Kentaroh Honda ◽  
Masashi Yanagisawa
Keyword(s):  
Neural Activity ◽  
Fiber Optic ◽  
Fluorescent Imaging ◽  
Freely Moving

scholarly journals All-optical imaging and patterned stimulation with a one-photon endoscope

2021 ◽  
Author(s):  
Jinyong Zhang ◽  
Ryan N Hughes ◽  
Namsoo Kim ◽  
Isabella P Fallon ◽  
Konstantin I bakhurin ◽  
...  
Keyword(s):  
Neural Activity ◽  
Calcium Imaging ◽  
Neural Circuit ◽  
Integrated System ◽  
Striatal Neurons ◽  
Indirect Pathway ◽  
Cellular Resolution ◽  
All Optical ◽  
Freely Moving

While in vivo calcium imaging makes it possible to record activity in defined neuronal populations with cellular resolution, optogenetics allows selective manipulation of neural activity. Recently, these two tools have been combined to stimulate and record neural activity at the same time, but current approaches often rely on two-photon microscopes that are difficult to use in freely moving animals. To address these limitations, we have developed a new integrated system combining a one-photon endoscope and a digital micromirror device for simultaneous calcium imaging and precise optogenetic photo-stimulation with near cellular resolution (Miniscope with All-optical Patterned Stimulation and Imaging, MAPSI). Using this highly portable system in freely moving mice, we were able to image striatal neurons from either the direct pathway or the indirect pathway while simultaneously activating any neuron of choice in the field of view, or to synthesize arbitrary spatiotemporal patterns of photo-stimulation. We could also select neurons based on their relationship with behavior and recreate the behavior by mimicking the natural neural activity with photo-stimulation. MAPSI thus provides a powerful tool for interrogation of neural circuit function in freely moving animals.

Multi-Fiber Photometry to Record Neural Activity in Freely-Moving Animals

10.3791/60278 ◽  
2019 ◽  
Author(s):  
Ekaterina Martianova ◽  
Sage Aronson ◽  
Christophe D. Proulx
Keyword(s):  
Neural Activity ◽  
Freely Moving

scholarly journals Ubiquitous proximity to a critical state for collective neural activity in the CA1 region of freely moving mice

2022 ◽  
Author(s):  
Yi-Ling Chen ◽  
Chun-Chung Chen ◽  
Yu-Ying Mei ◽  
Ning Zhou ◽  
Dongchuan Wu ◽  
...  
Keyword(s):  
Neural Activity ◽  
Critical State ◽  
Ca1 Region ◽  
Freely Moving

scholarly journals Large-scale cellular-resolution imaging of neural activity in freely behaving mice

2021 ◽  
Author(s):  
D.P. Leman ◽  
I.A. Chen ◽  
K.A. Bolding ◽  
J. Tai ◽  
L.K. Wilmerding ◽  
...  
Keyword(s):  
Neural Activity ◽  
Large Scale ◽  
First Generation ◽  
Brain Regions ◽  
Field Of View ◽  
Cellular Resolution ◽  
Neural Populations ◽  
Resolution Imaging ◽  
Freely Behaving ◽  
Freely Moving

AbstractMiniaturized microscopes for head-mounted fluorescence imaging are powerful tools for visualizing neural activity during naturalistic behaviors, but the restricted field of view of first-generation ‘miniscopes’ limits the size of neural populations accessible for imaging. Here we describe a novel miniaturized mesoscope offering cellular-resolution imaging over areas spanning several millimeters in freely moving mice. This system enables comprehensive visualization of activity across entire brain regions or interactions across areas.

scholarly journals Simultaneous optogenetic manipulation and calcium imaging in freely movingC. elegans

2013 ◽  
Author(s):  
Frederick B. Shipley ◽  
Christopher M. Clark ◽  
Mark J. Alkema ◽  
Andrew M. Leifer
Keyword(s):  
Neural Activity ◽  
Calcium Imaging ◽  
Neural Circuits ◽  
Neural Response ◽  
Sensory Stimuli ◽  
Systems Neuroscience ◽  
C Elegans ◽  
Fundamental Goal ◽  
Freely Moving ◽  
Interneuron Activity

A fundamental goal of systems neuroscience is to probe the dynamics of neural activity that drive behavior. Here we present an instrument to simultaneously manipulate neural activity via Channelrhodopsin, monitor neural response via GCaMP3, and observes behavior in freely moving C. elegans. We use the instrument to directly observe the relation between sensory stimuli, interneuron activity and locomotion in the mechanosensory circuit. Now published as: Front Neural Circuits 8:28, doi:10.3389/fncir.2014.00028

scholarly journals Optogenetic control of selective neural activity in multiple freely moving Drosophila adults

2014 ◽  
Vol 111 (14) ◽  
pp. 5367-5372 ◽  
Author(s):  
M.-C. Wu ◽  
L.-A. Chu ◽  
P.-Y. Hsiao ◽  
Y.-Y. Lin ◽  
C.-C. Chi ◽  
...  
Keyword(s):  
Neural Activity ◽  
Freely Moving ◽  
Optogenetic Control

scholarly journals Cocaine addicted rats show reduced neural activity as revealed by manganese-enhanced MRI

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Nazzareno Cannella ◽  
Alejandro Cosa-Linan ◽  
Tatiane Takahashi ◽  
Wolfgang Weber-Fahr ◽  
Rainer Spanagel
Keyword(s):  
Neuronal Activity ◽  
Neural Activity ◽  
Brain Activity ◽  
Cocaine Addiction ◽  
Resonance Imaging ◽  
Experimental Conditions ◽  
Chronic Cocaine ◽  
Potential Biomarker ◽  
Freely Moving ◽  
Manganese Enhanced Mri

Abstract Cocaine addiction develops as a continuum from recreational to habitual and ultimately compulsive drug use. Cocaine addicts show reduced brain activity. However, it is not clear if this condition results from individual predisposing traits or is the result of chronic cocaine intake. A translational neuroimaging approach with an animal model distinguishing non-addict-like vs. addict-like animals may help overcome the limitations of clinical research by comparing controlled experimental conditions that are impossible to obtain in humans. Here we aimed to evaluate neuronal activity in freely moving rats by manganese enhanced magnetic resonance imaging in the 0/3crit model of cocaine addiction. We show that addict-like rats exhibit reduced neuronal activity compared to cocaine-naïve controls during the first week of abstinence. In contrast, cocaine-experienced non-addict-like rats maintained their brain activity at a level comparable to cocaine-naïve controls. We also evaluated brain activity during cocaine bingeing, finding a general reduction of brain activity in cocaine experienced rats independent of an addiction-like phenotype. These findings indicate that brain hypoactivity in cocaine addiction is associated with the development of compulsive use rather than the amount of cocaine consumed, and may be used as a potential biomarker for addiction that clearly distinguishes non-addict-like vs addict-like cocaine use.

Sign in / Sign up

Export Citation Format

Share Document