scholarly journals Bridge-Overlap-Extension PCR Method for Constructing Chimeric Genes

BioTechniques ◽  
1999 ◽  
Vol 26 (6) ◽  
pp. 1082-1086 ◽  
Author(s):  
Raj Kumar Mehta ◽  
Jagmohan Singh
Keyword(s):  
Chimeric Genes ◽  
Overlap Extension Pcr ◽  
Overlap Extension ◽  
Pcr Method

scholarly journals Reliable Scarless Fusion of Genes through Overlap Extension PCR to Develop Chimeric Genes

2019 ◽  
Vol 51 (4) ◽  
Author(s):  
Iram Gull ◽  
Muhammad Shahbaz Aslam ◽  
Imran Tipu ◽  
Roohi Mushtaq ◽  
Muhammad Amin Athar
Keyword(s):  
Chimeric Genes ◽  
Overlap Extension Pcr ◽  
Overlap Extension

scholarly journals Efficient site-directed mutagenesis using an overlap extension-PCR method for expressing Mycoplasma hyopneumoniae genes in Escherichia coli

2009 ◽  
Vol 79 (1) ◽  
pp. 101-105 ◽  
Author(s):  
Simone Simionatto ◽  
Silvana B. Marchioro ◽  
Vanessa Galli ◽  
Tessália D. Luerce ◽  
Daiane D. Hartwig ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Mycoplasma Hyopneumoniae ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Overlap Extension Pcr ◽  
Overlap Extension ◽  
Pcr Method

Assembling and cloning genes for fusion proteins using reverse transcription one-step overlap extension PCR method

2006 ◽  
Vol 351 (2) ◽  
pp. 311-313 ◽  
Author(s):  
Zsolt B. Nagy ◽  
Zoltán Varga-Orvos ◽  
Barnabás Szakál ◽  
László Tamás ◽  
László G. Puskás
Keyword(s):  
Reverse Transcription ◽  
Fusion Proteins ◽  
Overlap Extension Pcr ◽  
Overlap Extension ◽  
One Step ◽  
Pcr Method

scholarly journals Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Hasnain Hussain ◽  
Nikson Fatt-Ming Chong
Keyword(s):  
Codon Optimization ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Combined Method ◽  
Base Pairs ◽  
Single Experiment ◽  
Mutagenic Primer ◽  
Overlap Extension Pcr ◽  
Overlap Extension ◽  
Pcr Method

The combined overlap extension PCR (COE-PCR) method developed in this work combines the strengths of the overlap extension PCR (OE-PCR) method with the speed and ease of the asymmetrical overlap extension (AOE-PCR) method. This combined method allows up to 6 base pairs to be mutated at a time and requires a total of 40–45 PCR cycles. A total of eight mutagenesis experiments were successfully carried out, with each experiment mutating between two to six base pairs. Up to four adjacent codons were changed in a single experiment. This method is especially useful for codon optimization, where doublet or triplet rare codons can be changed using a single mutagenic primer set, in a single experiment.

scholarly journals Aptamer-dependent full-length cDNA synthesis by overlap extension PCR

BioTechniques ◽  
2004 ◽  
Vol 37 (1) ◽  
pp. 124-129 ◽  
Author(s):  
Yasumasa Mitani ◽  
Takayuki Nakayama ◽  
Matthias Harbers ◽  
Yoshihide Hayashizaki
Keyword(s):  
Full Length ◽  
Cdna Synthesis ◽  
Full Length Cdna ◽  
Overlap Extension Pcr ◽  
Overlap Extension

A Combination Strategy for Construction of Peptide-��2m-H-2Kb Single Chain with Overlap Extension PCR and One-Step Cloning

2016 ◽  
Vol 26 (12) ◽  
pp. 2184-2191 ◽  
Author(s):  
Tao Xu ◽  
Xiaoe Li ◽  
You Wu ◽  
Khawar Ali Shahzad ◽  
Wei Wang ◽  
...  
Keyword(s):  
Single Chain ◽  
Combination Strategy ◽  
Overlap Extension Pcr ◽  
Overlap Extension ◽  
One Step
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