Activities of methionine-γ-lyase in the acidophilic archaeon “Ferroplasma acidarmanus” strain fer1

‘Ferroplasma acidarmanus’ strain Fer1 is an extremely acidophilic archaeon involved in the genesis of acid mine drainage, and was isolated from copper-contaminated mine solutions at Iron Mountain, CA, USA. Here, the initial proteomic and molecular investigation of Cu2+ resistance in this archaeon is presented. Analysis of Cu2+ toxicity via batch growth experiments and inhibition of oxygen uptake in the presence of ferrous iron demonstrated that Fer1 can grow and respire in the presence of 20 g Cu2+ l−1. The Fer1 copper resistance (cop) loci [originally detected by Ettema, T. J. G., Huynen, M. A., de Vos, W. M. & van der Oost, J. Trends Biochem Sci 28, 170–173 (2003)] include genes encoding a putative transcriptional regulator (copY), a putative metal-binding chaperone (copZ) and a putative copper-transporting P-type ATPase (copB). Transcription analyses demonstrated that copZ and copB are co-transcribed, and transcript levels were increased significantly in response to exposure to high levels of Cu2+, suggesting that the transport system is operating for copper efflux. Proteomic analysis of Fer1 cells exposed to Cu2+ revealed the induction of stress proteins associated with protein folding and DNA repair (including RadA, thermosome and DnaK homologues), suggesting that ‘Ferroplasma acidarmanus’ Fer1 uses multiple mechanisms for resistance to high levels of copper.

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Methionine gamma lyase from Clostridium sporogenes increases the anticancer effect of doxorubicin in A549 cells and human cancer xenografts

Investigational New Drugs ◽

10.1007/s10637-018-0619-4 ◽

2018 ◽

Vol 37 (2) ◽

pp. 201-209 ◽

Cited By ~ 2

Author(s):

V. S. Pokrovsky ◽

N. Yu Anisimova ◽

D. Zh Davydov ◽

S. V. Bazhenov ◽

N. V. Bulushova ◽

...

Keyword(s):

Human Cancer ◽

A549 Cells ◽

Anticancer Effect ◽

Clostridium Sporogenes ◽

Methionine Gamma Lyase

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Purification and characterization of methionine γ-lyase from Trichomonas vaginalis

Biochemical Journal ◽

10.1042/bj2790675 ◽

1991 ◽

Vol 279 (3) ◽

pp. 675-682 ◽

Cited By ~ 65

Author(s):

B C Lockwood ◽

G H Coombs

Keyword(s):

Molecular Mass ◽

Trichomonas Vaginalis ◽

Protozoan Parasite ◽

Purification And Characterization ◽

Native Molecular Mass ◽

Substrate Analogues ◽

Beta Elimination ◽

Derivatives Of ◽

Methionine Gamma Lyase

Methionine gamma-lyase (EC 4.4.1.11) was purified to homogeneity from the anaerobic protozoan parasite Trichomonas vaginalis by a series of f.p.l.c. procedures. The enzyme catalyses alpha gamma- and alpha beta-elimination reactions of a number of derivatives of methionine and cysteine. It also catalyses gamma-replacement reactions of the thiomethyl group of methionine, homocysteine and ethionine to yield the corresponding S-substituted homocysteine derivative. The enzyme is pyridoxal 5′-phosphate-dependent, has a native molecular mass of approx. 160 kDa and consists of four apparently identical subunits of molecular mass 43-45 kDa. The absorption spectrum of the enzyme is typical of those obtained for other pyridoxal 5′-phosphate-dependent enzymes, and the holoenzyme can be resolved to the apoenzyme by incubation with hydroxylamine and reconstituted by addition of the cofactor. The enzyme activity is significantly affected by carbonyl and thiol reagents, is competitively inhibited by a number of substrate analogues and is completely inactivated by the suicide inhibitor DL-propargylglycine. The T. vaginalis enzyme is similar, in terms of activity and properties, to the enzymes found in a number of species of bacteria that metabolize methionine under anaerobic conditions. It is suggested that methionine catabolism may be of particular importance to the survival of T. vaginalis under microaerophilic conditions in its host.

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Microbial Population of Industrial Biooxidation Reactors

Solid State Phenomena ◽

10.4028/www.scientific.net/ssp.262.48 ◽

2017 ◽

Vol 262 ◽

pp. 48-52 ◽

Cited By ~ 7

Author(s):

Aleksander Bulaev ◽

Aleksandr Belyi ◽

Anna Panyushkina ◽

Natalia Solopova ◽

Tatyana Pivovarova

Keyword(s):

16S Rrna ◽

Microbial Population ◽

Acid Soils ◽

Rrna Gene ◽

Acidithiobacillus Thiooxidans ◽

Nutrient Media ◽

Molecular Biological Analysis ◽

Sulfide Ore ◽

Ferroplasma Acidarmanus ◽

Gold Bearing

Microbial population performing biooxidation of flotation concentrate of gold bearing sulfide ore containing pyrite, arsenopyrite, and pyrrhotite was studied using cultural and molecular biological (metagenomics sequencing of V3-V4 fragments of 16S rRNA gene) approaches. The biooxidation of the concentrate was conducted at temperatures from 38 to 42°C. Strains of Acidithiobacillus thiooxidans, Acidiphilium multivorum, Leptospirillum ferriphilum, Sulfobacillus thermotolerans, Ferroplasma acidarmanus, and Ferroplasma acidiphilum were isolated from the samples of the pulp from biooxidation reactors. It was shown that optimum temperatures of isolated strains were from 38 to 40°C. Metagenomic analysis demonstrated predominance of the genera Acidiferrobacter, Acidithiobacillus, Acidiphilum, Leptospirillum, and Ferroplasma. According to results of molecular biological analysis, share of the genus Acidithiobacillus was of 0 to 25%, share of the genus Acidiferrobacter was of 7 to 56%, share of the genus Acidiphilum was of 0.03 to 36%, share of the genus Leptospirillum was of 0.7 to 7%, whereas share of the archaea of the genus Ferroplasma was of 33 to 94%. Thus, it was shown that representatives of the genus Ferroplasma can play significant role in bioleach process. Representatives of the genus Acidiferrobacter were previously detected in acid mine drainages, acid soils as well as in bioleach heaps and reactors, whereas data on predominance of the genus in tank bioleach processes have not been presented in the literature. In the present study, strains of the genus Acidiferrobacter were not isolated despite application of the nutrient media recommended for Acidiferrobacter and their properties were not studied. Nevertheless, results of the present study suggest that representatives of the genus Acidiferrobacter have a great impact on industrial bioleach processes.

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