A new method for the determination of dissociation constant (kd) on the binding of CA19-9 to its antibody in type 2diabetic patients by enzyme linked immunosorbent assay (ELISA) with some modifications.

In this study, a rapid and sensitive immunoassay method has been established based on calibration curve implanted enzyme-linked immunosorbent assay (C-ELISA) for the simultaneously quantitative determination of aflatoxin B1, deoxynivalenol and zearalenone in cereal samples, soybean and peanut. The C-ELISA avoids using the standard substances during the detection. The principle of the C-ELISA is to implant the optimized standard curve data into the matched analysis software which can make data processing more convenient and faster. The implanted calibration curve software was programmed with C plus plus. In the new immunoassay system for aflatoxin B1, deoxynivalenol and zearalenone, their linear detection ranges were from 0.03~0.81, 1.00~27.00 and 5.00~135.00 ng/g, respectively. Recovery rates from spiked samples ranged from 85% to 110% with the intra-assay coefficients of variation under 5%. Compared with HPLC method, the new method showed consistence in all the observed contents of the three mycotoxins in real samples. The new method can rapidly and reliably high throughput simultaneously screen for multiplex mycotoxins.

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EVALUATION OF A RECENTLY DEVELOPPED ENZYME LINKED IMMUNOSORBENT ASSAY FOR THE DETERMINATION OF ANTI-PHOSPHOLIPASE A2 RECEPTOR ANTIBODIES

10.26226/morressier.56e174d9d462b8028d88aca7 ◽

2016 ◽

Author(s):

Anne-Sophie Messiaen

Keyword(s):

Phospholipase A2 ◽

Immunosorbent Assay ◽

Enzyme Linked Immunosorbent Assay ◽

Phospholipase A2 Receptor ◽

Receptor Antibodies

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Biotin-avidin Mediated Competitive Enzyme-linked Immunosorbent Assay for Determination of Ketamine

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY (CHINESE VERSION) ◽

10.3724/sp.j.1096.2010.00117 ◽

2010 ◽

Vol 38 (1) ◽

pp. 117-120

Author(s):

Wen-Di ZHANG ◽

Ping SU ◽

Yi YANG ◽

Zhen-Quan GUO

Keyword(s):

Immunosorbent Assay ◽

Enzyme Linked Immunosorbent Assay

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SENSITIVE AND SPECIFIC ENZYME-LINKED IMMUNOSORBENT ASSAY FOR UROKINASE-TYPE PLASMINOGEN ACTIVATOR IN HUMAN PLASMA

10.1055/s-0038-1644425 ◽

1987 ◽

Cited By ~ 1

Author(s):

J Grøndahl-HANSEN ◽

N Agerlin ◽

L S Nielsen ◽

K Danø

Keyword(s):

Plasminogen Activator ◽

Immunosorbent Assay ◽

Enzyme Linked Immunosorbent Assay ◽

Mean Values ◽

Amino Terminal ◽

Type Plasminogen Activator ◽

Healthy Donors ◽

Urokinase Type Plasminogen Activator ◽

The Mean

An enzyme-linked immunosorbent assay (ELISA) was developed for the measurement of human urokinase-type plasminogen activator (u-PA) in plasma and serum. Microtiter plates were coated with a monoclonal antibody and incubated with standard or sample. Bound u-PA was quantitated with polyclonal antibodies conjugated with biotin, followed by avidin-peroxidase. The assay was 10-fold as sensitive as other previously reported ELISAs, the detection limit being approximately 1 pg of u-PA in a volume of 100 μl with a linear dose-response up to 15 pg of u-PA. The assay detected active u-PA and its inactive proenzyme form equally well and the recovery of both forms was higher than 90% in plasma. A variety of structurally related proteins, including t-PA, were tested, but no reaction with proteins other than u-PA and its amino-terminal degradation product were observed. The intra-assay and inter-assay coefficients of variation for determination of u-PA in plasma were 7.6% and 8.4%, respectively. The assay was equally applicable to serum. The values obtained with plasma and serum were similar, and the results were not affected by small variations in the preparation of the samples. The ELISA was used to measure the concentration of u-PA in plasma from 34 healthy donors. The mean values for u-PA in plasma from healthy donors was 1.1 ng/ml ± 0.3 ng/ml (SD) (range 0.6 - 1.5 ng/ml). No significant differences were found between men and women and no correlation between u-PA concentration and age could be demonstrated.The mean u-PA concentration in plasma from healthy donors obtained in this study is substantially lower than that reported by others. This might be due to different methods of determination of the protein content of the standard preparations or to differences in the specificity of the assays.

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