scholarly journals Resistance elicitors and defense response enhancers of maize to Spodoptera frugiperda (J.E.Smith) (Lepidoptera: Noctuidae)

2019 ◽  
pp. 1001-1008
Author(s):  
Janaina Marques Mondego ◽  
Raimunda Nonata Santos de Lemos ◽  
Jacinto de Luna Bastista ◽  
Daniele Lavra Vieira ◽  
Kedma Maria Pinto ◽  
...  
Keyword(s):  
Polyphenol Oxidase ◽  
Defense Response ◽  
Oxidase Activity ◽  
Block Design ◽  
Positive Control ◽  
Negative Control ◽  
Potassium Silicate ◽  
Polyphenol Oxidase Activity ◽  
Damage Level ◽  
Corn Plants

The objective of this research was to assess the potential of abiotic compounds as defense response enhancers in maize using enzymatic quantification and their efficiency in reduction of damages caused by S. frugiperda. The experiment was laid out in a randomized complete block design with six treatments and five replications. The treatments were: T1: positive control (distilled water + infestation); T2: negative control (without product application, uninfested); T3: biofertilizer; T4: acibenzolar-S-methyl (ASM); T5: potassium silicate; T6: potassium silicate + ASM. The treatments were applied in V6 stage of maize plants. After five days, the plants were artificially infested with 2nd instar caterpillars of S. frugiperda. Damage assessments were carried out at 4, 8, 16 and 22 days after infestation using a scale of notes. To evaluate the enzymatic activity, one leaf of each plant was removed at 2, 4, 8, 16 and 22 days after infestation. The largest peroxidase activities were observed at two DAI (days after infestation) using the potassium silicate + ASM (2,344.12 UAE.mg-1 of proteína.min-1) treatment, while the larger polyphenol oxidase activity peaks were observed after treatment with ASM at 22 DAI. The application of potassium silicate + ASM in corn plants can promote peroxidase activity. The polyphenol oxidase activity was increased after application of ASM, whereas the presence of the pest (on positive control) could potentially increase the activity of phenylalanine ammonia-lyase. The applications of ASM alone and potassium silicate + ASM contributed to reduction of the foliar damage level caused by S. frugiperda in corn plants.

Polyphenol oxidase activity staining in polyacrylamide electrophoresis gels

1997 ◽  
Vol 34 (2) ◽  
pp. 155-159 ◽  
Author(s):  
Antonio Rescigno ◽  
Francesca Sollai ◽  
Andrea C Rinaldi ◽  
Giulia Soddu ◽  
Enrico Sanjust
Keyword(s):  
Polyphenol Oxidase ◽  
Oxidase Activity ◽  
Polyphenol Oxidase Activity ◽  
Activity Staining ◽  
Polyacrylamide Electrophoresis

Mapping and allelic variations of Ppo-B1 and Ppo-B2 gene-related polyphenol oxidase activity in durum wheat

2015 ◽  
Vol 35 (2) ◽  
Author(s):  
Francesca Taranto ◽  
Giacomo Mangini ◽  
Antonella Pasqualone ◽  
Agata Gadaleta ◽  
Antonio Blanco
Keyword(s):  
Durum Wheat ◽  
Polyphenol Oxidase ◽  
Oxidase Activity ◽  
Polyphenol Oxidase Activity ◽  
Allelic Variations

scholarly journals Effects of hot-water and steam blanching of sliced potato on polyphenol oxidase activity

2018 ◽  
Vol 54 (2) ◽  
pp. 403-411 ◽  
Author(s):  
Roberto Moscetti ◽  
Flavio Raponi ◽  
Danilo Monarca ◽  
Giacomo Bedini ◽  
Serena Ferri ◽  
...  
Keyword(s):  
Polyphenol Oxidase ◽  
Oxidase Activity ◽  
Hot Water ◽  
Polyphenol Oxidase Activity

scholarly journals Effect of Some Citrus Essential Oils on Post-Harvest Shelf Life and Physicochemical Quality of Strawberries during Cold Storage

Agronomy ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1466
Author(s):  
Said A. Shehata ◽  
Emad A. Abdeldaym ◽  
Marwa R. Ali ◽  
Reda M. Mohamed ◽  
Rwotonen I. Bob ◽  
...  
Keyword(s):  
Weight Loss ◽  
Essential Oils ◽  
Shelf Life ◽  
Polyphenol Oxidase ◽  
Oxidase Activity ◽  
Soluble Solids ◽  
Soluble Solids Content ◽  
Polyphenol Oxidase Activity ◽  
Total Antioxidant ◽  
Solids Content

Utilization of essential oils alone or incorporation with edible films is an appropriate technique to conserve the quality attributes and reduce post-harvest deterioration in fresh vegetables and fruits. Strawberries, being perishable fruits have a short shelf life, and using essential oils is considered one of the most suitable methods to prolong their shelf life during storage. The current study assessed the impact of different essential oils, including lemon oil (L), orange oil (O) and mandarin oil (M) on the physicochemical and microbial load of strawberries (Fragaria × ananassa cv. Festival) stored at 2 ± 1 °C and 95% relative humidity (RH) for 18 days. The differences in the physicochemical and microbial properties of strawberries were assessed by determining the following parameter changes: weight loss, decay percentage, firmness, soluble solids content, titratable acidity, color, anthocyanins, vitamin C, total phenol, total antioxidant, catalase activity, polyphenol oxidase activity, sensory evaluation, microbial content, total coliforms, molds, and yeasts. The results of this study indicated that the fruits treated with all essential oils treatments (L, O and M) had higher total antioxidant content and physicochemical properties than untreated fruits, due to protection against the microbial growth of molds, and yeasts. At the end of the storage period, the treated fruits showed a greater acceptance and sensory attributes than the untreated fruits. Furthermore, the correlation study showed a significant and negative relationship between the total antioxidant of treated fruits and following quality attributes including, weight loss, decay percentage, respiration rate soluble solids content, polyphenol oxidase activity, molds, and yeasts. It is noteworthy that all the essential oil treatments extended the shelf-life of strawberries and delayed their deterioration up to 18 days.

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