MtNOOT gene enhanced high productivity and economical characteristics of tomato (Lycopersicon esculentum)

Plant Omics ◽

10.21475/poj.14.01.21.p3141 ◽

2021 ◽

pp. 1-10

Author(s):

Ghada Ahmed Abu El-Heba

Keyword(s):

Phenolic Compounds ◽

Lycopersicon Esculentum ◽

Transgenic Tomato ◽

Wild Type ◽

Tomato Fruits ◽

Tomato Plants ◽

High Productivity ◽

Transgenic Tomato Plants ◽

Fruit Productivity ◽

Long Time

Tomato (Lycopersicon esculentum) is the main vegetal crop that has tremendous popularity around the world. Medicago truncatula NOOT gene (Mt-NOOT) encodes a BTB/POZ-ankyrin repeat protein of the NONEXPRESSOR OF PR GENES1 (NPR1 family). It was introduced into Lycopersicon esculentum (Tomato) genome. The tomato plants that ectopically expressed Mt-NOOT obtained several favorable traits and fruit quality. Heteroblasty between the transgenic and the non-transgenic tomato leaves and flower architecture were used to distinguish transgenic and wild lines. Transgenic tomato plants accumulated a significant amount of phenolic compounds and plant pigmentations compared to the wild type. On the other hand, transgenic plants acquired a considerable amount of antioxidant such as CuZnSO superoxide dismutase (SOD), tomato Catalase (CAT), and tomato Cell wall-associated peroxidase (TPX1) than the wild type. Antioxidant high content together with the high content of phenolic compounds enabled the transgenic tomato fruits to gain not only edible benefits, but also a significant higher shelf-time, extended to six months more than the wild type stored at 25°C in dark and dry condition. Surprisingly, transgenic tomato fruits did not show any rotten process during long time storage as they did not acquire any contagious microorganism. Total fruit productivity in transgenic tomato was greater than the control with an estimated ratio of 84%.

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Transgenic Plants Expressing Geminivirus Movement Proteins: Abnormal Phenotypes and Delayed Infection by Tomato mottle virus in Transgenic Tomatoes Expressing the Bean dwarf mosaic virus BV1 or BC1 Proteins

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi.2000.13.3.297 ◽

2000 ◽

Vol 13 (3) ◽

pp. 297-308 ◽

Cited By ~ 54

Author(s):

Yu-Ming Hou ◽

Rick Sanders ◽

Virgina M. Ursin ◽

Robert L. Gilbertson

Keyword(s):

Transgenic Plants ◽

Mosaic Virus ◽

Viral Disease ◽

Transgenic Tomato ◽

Wild Type ◽

Significant Delay ◽

Tomato Plants ◽

Movement Proteins ◽

Transgenic Tomato Plants ◽

Bean Dwarf Mosaic Virus

Transgenic tomato plants expressing wild-type or mutated BV1 or BC1 movement proteins from Bean dwarf mosaic virus (BDMV) were generated and examined for phenotypic effects and resistance to Tomato mottle virus (ToMoV). Fewer transgenic plants were recovered with the wild-type or mutated BC1 genes, compared with the wild-type or mutated BV1 genes. Transgenic tomato plants expressing the wild-type or mutated BV1 proteins appeared normal. Interestingly, although BDMV induces only a symptomless infection in tomato (i.e., BDMV is not well adapted to tomato), transgenic tomato plants expressing the BDMV BC1 protein showed a viral disease-like phenotype (i.e., stunted growth, and leaf mottling, curling, and distortion). This suggests that the symptomless phenotype of BDMV in tomato is not due to a host-specific defect in the BC1 protein. One transgenic line expressing the BC1 gene did not show the viral disease-like phenotype. This was associated with a deletion in the 3′ region of the gene, which resulted in expression of a truncated BC1 protein. Several R0 plants, expressing either wild-type or mutated BV1 or BC1 proteins, showed a significant delay in ToMoV infection, compared with non-transformed plants. R1 progeny plants also showed a significant delay in ToMoV infection, but this delay was less than that in the R0 parents. These results also demonstrate that expression of viral movement proteins, in transgenic plants, can have deleterious effects on various aspects of plant development.

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Overexpression of PbDHAR2 from Pyrus sinkiangensis in Transgenic Tomato Confers Enhanced Tolerance to Salt and Chilling Stresses

HortScience ◽

10.21273/hortsci.50.6.789 ◽

2015 ◽

Vol 50 (6) ◽

pp. 789-796 ◽

Cited By ~ 8

Author(s):

An Qin ◽

Xiaosan Huang ◽

Huping Zhang ◽

Juyou Wu ◽

Jie Yang ◽

...

Keyword(s):

Ascorbic Acid ◽

Chilling Tolerance ◽

Antioxidant Enzyme Activity ◽

Transgenic Tomato ◽

Wild Type ◽

Tomato Plants ◽

Chlorophyll Contents ◽

Transgenic Tomato Plants ◽

Transgenic Lines ◽

Redox Buffer

Ascorbic acid (AsA) is a major antioxidant and redox buffer in plants. Dehydroascorbate reductase (DHAR; EC 1.8.5.1) catalyzes the conversion of dehydroascorbate (DHA) to AsA and is crucial for AsA regeneration. In this study, we developed transgenic tomato plants that overexpressed PbDHAR2 to investigate whether PbDHAR2 could limit the deleterious effects of salt and chilling stresses. These transgenic plants contained significantly higher AsA levels than the wild-type (WT) plants. Overexpression of PbDHAR2 increased the expression of the AsA-glutathione (GSH) cycle genes in transgenic lines under salt and chilling stresses. In addition, the transgenic lines subjected to salt and chilling stresses showed higher levels of antioxidant enzyme activity, lower malondialdehyde (MDA) levels, and higher chlorophyll contents than the WT. Thus, our results demonstrate that the regulation of PbDHAR2 during AsA regeneration contributes to enhanced salt and chilling tolerance in tomato.

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