scholarly journals Experience of using a thrombofibrin clot of plateletrich plasma in ulcerative lesions of the cornea

2022 ◽  
Vol 14 (4) ◽  
pp. 15-21
Author(s):  
E. V. Fedoseeva ◽  
E. V. Chentsova ◽  
N. V. Borovkova ◽  
I. N. Ponomarev ◽  
V. A. Vlasova ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Platelet Rich Plasma ◽  
Autologous Blood ◽  
Complete Healing ◽  
Autologous Serum ◽  
Amniotic Membrane Transplantation ◽  
Corneal Ulcers ◽  
Severe Burns ◽  
Surgical Methods ◽  
Extensive Damage

Purpose: to study the effectiveness of the use of thrombofibrin clot of platelet-rich plasma (PRP) in patients with corneal ulcers. Material and methods. A clinical study, conducted by the Department of Traumatology and Reconstructive Surgery of Helmholtz National Medical Research Center of Eye Diseases, involved 20 patients, aged from 22 to 82, with corneal ulcers of inflammatory and burn genesis more than 100 microns deep. All patients got coated with a thrombofibrin PRP clot from autologous blood. Prior to the study, all patients received standard treatment for 2 weeks to 3 months, including multiple amniotic membrane coating, with no effect. The thrombofibrin clot was produced by the Scientific Department of Biotechnology and Transfusiology of the N.V. Sklifosovsky Research Institute for Emergency Medicine. The ready clot was placed on the surface of the cornea and covered with an amniotic membrane. The membrane was fixed to the episclera along the border of the limb with a circular suture, whereupon autologous serum was injected along the limb in 4 quadrants, to be followed by temporary lateral blepharography. Results. On the 2nd day following the procedure, the patients noted a decrease in lacrimation and pain in the operated eye. As shown by optical coherence tomography, the average depth of the corneal ulcer at the beginning of the study in all patients was 129 ± 28.5 microns. On the 5th day, the depth lowered to an average of 71 ± 32.6 microns, and on the 10th day, to 23.3 ± 15.1 microns. In 7 patients (35%), complete healing of the defect was observed on the 15th day, while in 9 patients (45%) it was stated between the 16th and the 20th day. Thus, the average time of healing of the ulcer with complete epithelization occurred was 15 days. In four patients with the consequences of severe burns (20%), the ulcer did not heal due to extensive damage to the limbal zone. Conclusion. The use of a thrombofibrin PRP clot in combination with amniotic membrane transplantation allows achieving a stable and fairly rapid healing of corneal ulcers of various origins. However, this method is ineffective in patients with limbal cell insufficiency, severe burns and extensive damage to the limbal zone. In such cases, it is advisable to use more radical surgical methods, such as buccal or limbal cell transplantation, or allolimbal transplantation.

scholarly journals Comparison of amniotic membrane transplantation with and without cultured limbal epithelium for persistent corneal ulcers

2021 ◽  
Vol 35 (1) ◽  
pp. 739-745
Author(s):  
Rozaliya Hristova ◽  
Yani Zdravkov ◽  
Georgi Markov ◽  
Davide Borroni ◽  
Alexander Oscar ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Amniotic Membrane Transplantation ◽  
Corneal Ulcers ◽  
Limbal Epithelium

Role of multilayered amniotic membrane transplantation for the treatment of resistant corneal ulcers in North India

2013 ◽  
Vol 34 (3) ◽  
pp. 485-491 ◽  
Author(s):  
Shalini Mohan ◽  
Ina Budhiraja ◽  
Amit Saxena ◽  
Perwez Khan ◽  
S. K. Sachan
Keyword(s):  
Amniotic Membrane ◽  
North India ◽  
Amniotic Membrane Transplantation ◽  
Corneal Ulcers

Sutureless “Contact Lens Sandwich” Technique for Amniotic Membrane Therapy of Central Corneal Ulcers

2021 ◽  
pp. 112067212110490
Author(s):  
Saverio V. Luccarelli ◽  
Edoardo Villani ◽  
Stefano Lucentini ◽  
Francesco Bonsignore ◽  
Matteo Sacchi ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Contact Lens ◽  
Low Cost ◽  
Healing Time ◽  
Amniotic Membrane Transplantation ◽  
Recurrence Rates ◽  
Sandwich Technique ◽  
Corneal Ulcers ◽  
Bandage Contact Lens ◽  
Ring Complex

Purpose To describe a new technique for sutureless and glue-free amniotic membrane transplantation (AMT) and to investigate its effectiveness to treat corneal persistent epithelial defects (PEDs), compared to bandage contact lens (BCL) application alone. Methods We performed AMT with “contact lens sandwich technique” (CLS-AMT) in 8 consecutive patients with central/para-central (up to 4.00 mm from the geometrical centre) PED/ulceration and we retrospectively compared the results with 11 BCL procedures. Results The procedures were performed successfully with no complications. CLS-AMT showed significantly shorter healing time than BCL (24.0  ±  19.1 vs 42.9  ±  14.6 days; P < 0.05, Mann-Whitney test). Recurrence rates were 12% and 27% for CLS-AMT and BCL, respectively. Conclusion CLS-AMT technique, based on the suction effect due to the superposition of a bandage contact lens on the AM-ring complex, represents a quick, low cost, easy to perform and nearly non-invasive AMT technique. This approach is able to provide adequate fixation of AM, and it seems to be a safe and effective treatment for patients with PEDs.

A multicenter retrospective study on cryopreserved amniotic membrane transplantation for the treatment of complicated corneal ulcers in the dog

2019 ◽  
Vol 22 (5) ◽  
pp. 695-702 ◽  
Author(s):  
Daniel Costa ◽  
Marta Leiva ◽  
Fernando Sanz ◽  
Victoria Espejo ◽  
Javier Esteban ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Amniotic Membrane ◽  
Amniotic Membrane Transplantation ◽  
Corneal Ulcers

scholarly journals Efficacy of Amniotic Membrane Transplantation for the Treatment of Corneal Ulcers

Cornea ◽  
2020 ◽  
Vol 39 (4) ◽  
pp. 479-483 ◽  
Author(s):  
Kaspar Schuerch ◽  
Andrea Baeriswyl ◽  
Beatrice E. Frueh ◽  
Christoph Tappeiner
Keyword(s):  
Amniotic Membrane ◽  
Amniotic Membrane Transplantation ◽  
Corneal Ulcers

scholarly journals Active Pedicle Epithelial Flap Transposition Combined with Amniotic Membrane Transplantation for Treatment of Nonhealing Corneal Ulcers

2016 ◽  
Vol 2016 ◽  
pp. 1-7
Author(s):  
Ting Zhang ◽  
Yuexin Wang ◽  
Yanni Jia ◽  
Dongle Liu ◽  
Suxia Li ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Amniotic Membrane ◽  
Ulcer Healing ◽  
Laser Scanning ◽  
Corneal Ulcer ◽  
Anterior Segment ◽  
Healing Time ◽  
Stevens Johnson Syndrome ◽  
Amniotic Membrane Transplantation ◽  
Corneal Ulcers

Introduction. The objective was to evaluate the efficacy of active pedicle epithelial flap transposition combined with amniotic membrane transplantation (AMT) in treating nonhealing corneal ulcers.Material and Methods. Eleven patients (11 eyes) with nonhealing corneal ulcer who underwent the combined surgery were included. Postoperatively, ulcer healing time was detected by corneal fluorescein staining. Visual acuity, intraocular pressure, surgical complications, and recurrence were recorded. Corneal status was inspected by the laser scanning confocal microscopy and anterior segment optical coherence tomography (AS-OCT).Results. The primary diseases were herpes simplex keratitis (8 eyes), corneal graft ulcer (2 eyes), and Stevens-Johnson syndrome (1 eye). All epithelial flaps were intact following surgery, without shedding or displacement. Mean ulcer healing time was10.8±3.1days, with a healing rate of 91%. Vision significantly improved from 1.70 to 0.82 log MAR (P=0.001). A significant decrease in inflammatory cell infiltration and corneal stromal edema was revealed 2 months postoperatively by confocal microscopy and AS-OCT. Corneal ulcer recurred in 1 eye. None of the patients developed major complications.Conclusion. Active pedicle epithelial flap transposition combined with AMT is a simple and effective treatment for nonhealing corneal ulcers.

Amniotic Membrane Transplantation in Refractory Neurotrophic Corneal Ulcers

Cornea ◽  
2005 ◽  
Vol 24 (6) ◽  
pp. 654-660 ◽  
Author(s):  
Sudarshan Khokhar ◽  
Tanie Natung ◽  
Parul Sony ◽  
Namrata Sharma ◽  
Nutan Agarwal ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Amniotic Membrane Transplantation ◽  
Corneal Ulcers

scholarly journals A Challenging Management of Pseudomonas Perforated Corneal Ulcer with Multilayer Amniotic Membrane Transplantation and Pericardial Patch Graft in Pediatric Patient

2021 ◽  
Vol 1 (1) ◽  
pp. 22
Author(s):  
Nabilah Afifah ◽  
Herwindo Dicky Putranto ◽  
Lely Retno Wulandari
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Amniotic Membrane ◽  
Corneal Ulcer ◽  
Definitive Treatment ◽  
Amniotic Membrane Transplantation ◽  
Pericardial Patch ◽  
Corneal Perforation ◽  
Corneal Ulcers ◽  
Patch Graft ◽  
The Right

Introduction: Pseudomonas aeruginosa (P. aeruginosa) is the leading cause of corneal ulcers in children 0 to 3 years of age compared to children in general. Case presentation: A two-months-old infant presented with whitish patches on the right eye two days before admission. A central corneal ulcer with a size of 7-mm x 7-mm accompanied by corneal thinning and melting was shown on the right cornea. It is was surrounded by greyish white creamy infiltrates. Corneal scraping showed Pseudomonas aeruginosa specimens. The cornea became perforate and crystalline lens extrusion was found at the day after intravenous ceftriaxone, levofloxacin eye drop, and cefazoline fortified eye drop administering. It might be caused by bacterial elastase and toxin which contributed to corneal damage. The patient was underwent a multilayer Amniotic Membrane Transplantation (AMT) combined with a pericardial patch graft due to corneal perforation. Two months post-AMT and pericardial patch graft the corneal perforation became entirely heal due to multilayer AMT, despite lysis of the pericardial patch graft. Corneal scar formation and reduction of vitreous opacity in ultrasound examination were shown. The patient was planned to undergo keratoplasty. Conclusions: Corneal ulcers due to Pseudomonas aeruginosa are highly destructive. The levels of infection, diagnostic, and therapeutic are still problems in pediatric patients. Lens extrusion and lysis of the pericardial patch graft are examples in this case. Keratoplasty is the definitive treatment for corneal ulcers with perforation; however, multilayer AMT combined with pericardial patch graft can be used as an alternative therapy to accelerate wound healing, reduce inflammation, and maintain the integrity of the eyeball.

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