Experience of using a thrombofibrin clot of plateletrich plasma in ulcerative lesions of the cornea

Purpose: to study the effectiveness of the use of thrombofibrin clot of platelet-rich plasma (PRP) in patients with corneal ulcers. Material and methods. A clinical study, conducted by the Department of Traumatology and Reconstructive Surgery of Helmholtz National Medical Research Center of Eye Diseases, involved 20 patients, aged from 22 to 82, with corneal ulcers of inflammatory and burn genesis more than 100 microns deep. All patients got coated with a thrombofibrin PRP clot from autologous blood. Prior to the study, all patients received standard treatment for 2 weeks to 3 months, including multiple amniotic membrane coating, with no effect. The thrombofibrin clot was produced by the Scientific Department of Biotechnology and Transfusiology of the N.V. Sklifosovsky Research Institute for Emergency Medicine. The ready clot was placed on the surface of the cornea and covered with an amniotic membrane. The membrane was fixed to the episclera along the border of the limb with a circular suture, whereupon autologous serum was injected along the limb in 4 quadrants, to be followed by temporary lateral blepharography. Results. On the 2nd day following the procedure, the patients noted a decrease in lacrimation and pain in the operated eye. As shown by optical coherence tomography, the average depth of the corneal ulcer at the beginning of the study in all patients was 129 ± 28.5 microns. On the 5th day, the depth lowered to an average of 71 ± 32.6 microns, and on the 10th day, to 23.3 ± 15.1 microns. In 7 patients (35%), complete healing of the defect was observed on the 15th day, while in 9 patients (45%) it was stated between the 16th and the 20th day. Thus, the average time of healing of the ulcer with complete epithelization occurred was 15 days. In four patients with the consequences of severe burns (20%), the ulcer did not heal due to extensive damage to the limbal zone. Conclusion. The use of a thrombofibrin PRP clot in combination with amniotic membrane transplantation allows achieving a stable and fairly rapid healing of corneal ulcers of various origins. However, this method is ineffective in patients with limbal cell insufficiency, severe burns and extensive damage to the limbal zone. In such cases, it is advisable to use more radical surgical methods, such as buccal or limbal cell transplantation, or allolimbal transplantation.

Overtraining Strengthens the Visual Discrimination Memory Trace Outside the Hippocampus in Male Rats

The hippocampus (HPC) may compete with other memory systems when establishing a representation, a process termed overshadowing. However, this overshadowing may be mitigated by repeated learning episodes, making a memory resistant to post-training hippocampal damage. In the current study, we examined this overshadowing process for a hippocampal-dependent visual discrimination memory in rats. In Experiment 1, male rats were trained to criterion (80% accuracy on two consecutive days) on a visual discrimination and then given 50 additional trials distributed over 5 days or 10 weeks. Regardless of this additional learning, extensive damage to the HPC caused retrograde amnesia for the visual discrimination, suggesting that the memory remained hippocampal-dependent. In Experiment 2, rats received hippocampal damage before learning and required approximately twice as many trials to acquire the visual discrimination as control rats, suggesting that, when the overshadowing or competition is removed, the non-hippocampal memory systems only slowly acquires the discrimination. In Experiment 3, increasing the additional learning beyond criterion by 230 trials, the amount needed in Experiment 2 to train the non-hippocampal systems in absence of competition, successfully prevented the retrograde amnesic effects of post-training hippocampal damage. Combined, the findings suggest that a visual discrimination memory trace can be strengthened in non-hippocampal systems with overtraining and become independent of the HPC.

Iatrogenic damage in restorative tooth preparation and management

Iatrogenic damage in restorative teeth preparation and management constitute a significant issue that dentists and surgeons might face during and after restoration procedures. Many presentations can be observed for the injured enamel following iatrogenic damage as the presence of fine scratches, 1 mm wide vertical grooves, extensive damage and indentations. Following the occurrence of such complications, subsequent changes to the properties of the affected areas can significantly increase the risk of developing dental caries. It is essential to shedding more light on such phenomena to increase awareness among dentists and physicians and to enhance the potential outcomes. Accordingly, in this literature review, the aim was to discuss the iatrogenic damage in restorative teeth preparation and management as per evidence from the current studies in the literature. At first, the discussion was around the different types of iatrogenic injuries based on the affected regions and the incidence of adjacent teeth injury during restoration was high. Moreover, it had been discussed the potential mechanisms and contributing factors that might flare up the injury and increase the risk of significant damage induction. Hypersensitivity and using rough materials are two common factors that may induce inflammation and induce damage. Further investigations might be needed for the implication of safe practices for surgeons and dentists to enhance the outcomes.

Biological control of Western flower thrips, Frankliniella occidentalis using a self-sustaining granular fungal treatment

Western flower thrips (WFT), Frankliniella occidentalis, is one of the most destructive pests of vegetables, fruits and ornamental crops worldwide, causing extensive damage by direct feeding of the crop and transmitting economically important viruses. Despite the successes of biocontrol agents to control WFT, more efficient and cost-effective ways must be found to encourage grower adoption of integrated pest management. A sustainable fungal treatment was developed to preserve fungal inoculum in potting soil and reduce thrips populations. Combining cooked, oven-dried millet with BotaniGard® (a commercial form of Beauveria bassiana strain GHA) to potting soil increased spore production and persistence of the fungus in the soil. In treated pots with millet, spore concentrations were 3–4 times greater after 30 days compared with spore yields at 10 days. The number of WFT adults was significantly lower in the marigold pots treated with GHA mix + millet than untreated controls, 12% and 10% in treated pots and 70% and 68% in untreated pots in sterile and non-sterile soil, respectively. Incorporation of millet in the potting mix enhanced the effect of the fungal treatments by providing a nutritive substrate on which the fungus could become established. This method is relatively inexpensive and easy for growers to use in greenhouses because granular formulations of B. bassiana are not commercially available.

Environmental and Radar Characteristics of Gargantuan Hail-Producing Storms

Storms that produce gargantuan hail (defined here as ≥ 6 inches or 15 cm in maximum dimension), although seemingly rare, can cause extensive damage to property and infrastructure, and cause injury or even death to humans and animals. Currently, we are limited in our ability to accurately predict gargantuan hail and detect gargantuan hail on radar. In this study, we analyze the environments and radar characteristics of gargantuan hail-producing storms to define the parameter space of environments in which gargantuan hail occurs, and compare environmental parameters and radar signatures in these storms to storms producing other sizes of hail. We find that traditionally used environmental parameters used for severe storms prediction, such as most unstable convective available potential energy (MUCAPE) and 0–6 km vertical wind shear, display considerable overlap between gargantuan hail-producing storm environments and those that produce smaller hail. There is a slight tendency for larger MUCAPE values for gargantuan hail cases, however. Additionally, gargantuan hail-producing storms seem to have larger low-level storm-relative winds and larger updraft widths than those storms producing smaller hail, implying updrafts less diluted by entrainment and perhaps maximizing the liquid water content available for hail growth. Moreover, radar reflectivity or products derived from it are not different from cases of smaller hail sizes. However, inferred mesocyclonic rotational velocities within the hail growth region of storms that produce gargantuan hail are significantly stronger than the rotational velocities found for smaller hail categories.

Good Practices for In-situ Burning of Marshes Based on Two Decades of Responses in Louisiana

ABSTRACT Spills that result in oiled marshes provide unique challenges for responders because intensive removal methods can cause additional harm and slow overall recovery of the habitat. These issues are of particular concern for spills that affect the marsh interior, where access is limited, often resulting in extensive damage from foot and vessel traffic. In Louisiana, extensive marshes are crossed by numerous pipelines and oil wells, and spills can result in heavy oiling of interior habitats in remote locations. Thus, in-situ burning (ISB) is often considered as the best response option. Monitoring of in-situ burns in marshes has provided the scientific basis for evaluating the conditions under which a burn can speed recovery. The lessons learned from multiple burns in Louisiana over the period 2000–2019 include: the burned area can be much greater than oiled area, so the potential for a larger burn should be explicitly considered and planned for; a water layer over the marsh soil is preferred but not required under all conditions; water-saturated soils are required; ISB can be used weeks post-spill to remove oil, but it will not prevent vegetation mortality from oil exposure prior to the burn; oil that penetrates into the substrates or is released below the marsh surface may persist after burning; select ISB as an option early, to prevent damage from foot traffic, etc.; vegetative recovery usually occurs within 1–2 growing seasons; burning can result in a change in dominant plant species; and ISB is very appropriate for small spills in the marsh interior where access for manual removal can cause extensive damage.

Wildlife Conservation at a Garden Level: The Effect of Robotic Lawn Mowers on European Hedgehogs (Erinaceus europaeus)

We tested the effects of 18 models of robotic lawn mowers in collision with dead European hedgehogs and quantified the results into six damage categories. All models were tested on four weight classes of hedgehogs, each placed in three different positions. None of the robotic lawn mowers tested was able to detect the presence of dependent juvenile hedgehogs (<200 g) and all models had to touch the hedgehogs to detect them. Some models caused extensive damage to the hedgehog cadavers, but there were noteworthy differences in the degree of harm inflicted, with some consistently causing no damage. Our results showed that the following technical features significantly increased the safety index of the robotic lawn mowers: pivoting blades, skid plates, and front wheel drive. Based on these findings, we encourage future collaboration with the manufacturers of robotic lawn mowers to improve the safety for hedgehogs and other garden wildlife species.

Canine developmental elbow disease part 1: aetiopathogenesis and diagnosis

Elbow dysplasia or ‘developmental elbow disease’ as it is now known, is an umbrella term encompassing multiple abnormalities of the elbow joint. These include elbow incongruity, fragmented medial coronoid process, osteochondritis dissecans and ununited anconeal process. These problems may occur individually or in combination with each other and all may cause lameness, pain, reluctance to exercise and restricted movement as the disease progresses. The advanced stage of osteoarthritis associated with medial coronoid disease involving extensive damage to or loss of cartilage is known as medial compartment disease. There are multiple modalities available for imaging the elbow joint: radiography which is widely available in general practice can detect some changes but may miss others; computed tomography in conjunction with arthroscopic examination is considered the ‘gold standard’ in elbow imaging. Part 1 of this two part series of articles introduces the aetiopathogenesis of canine developmental elbow disease, and part 2 will cover the surgical and nonsurgical management.

Impact of COVID-19 on the future of cadaveric dissection and anatomy teaching

The COVID 19 pandemic has left a major impact on medical education globally. We are undergoing crisis for humanity with this virus causing extensive damage to life and its aspects. Hence, we do not know how this will unfold in upcoming year. All the academic classes are suspended during nationwide lockdown to ease the propagation. It is high time to rethink ways to deliver quality medical education under restriction of social distancing in real time teachings and discussions. We propose based upon our experiences, replacement of anatomy dissection with virtual dissection and its implications. It has its own challenges which could overcome with the planned directives based upon current experience.