scholarly journals A new way of concentrating the cellular material of exudates

2018 ◽  
pp. 166-168
Author(s):  
M. G. Leonov ◽  
V. I. Novik ◽  
S. A. Belyaeva ◽  
Ya. H.-B. Ershova ◽  
Zh. P. Selifonova
Keyword(s):  
Ovarian Cancer ◽  
Exposure Time ◽  
Cytological Study ◽  
Cellular Material ◽  
Optimal Time ◽  
Cytological Investigation ◽  
Minute Exposure ◽  
Cell Complexes ◽  
Sedimentation Method

Objective. Improve the method for concentrating the cellular material of serous cavities exudates for cytological investigation.Materials and methods. The optimal time of accumulation of cell samples in effusion liquids was determined on the basis of the sedimentation method using a dropping funnel by examining 28 samples of exudates (pleural and abdominal) obtained from 24 patients with ovarian cancer. The two methods for concentrating the cellular material of exudate were compared: the method using a dropping funnel and the method using a cylinder.Results. It was found that the 60-minute exposure time was optimal in using a dropping funnel to concentrate the cellular material of exudates.Conclusion. Using a dropping funnel to concentrate the cellular material of the exudates is optimal. In this case, the investigational preparations contain a sufficient amount of cellular material for the cytological study and the cell complexes are larger than those in using the cylinder.

Povidone-Iodine 3-Minute Exposure Time is Viable in Preparation for Cataract Surgery

2017 ◽  
Vol 27 (5) ◽  
pp. 573-576 ◽  
Author(s):  
Chu L. Nguyen ◽  
Lawrence J. Oh ◽  
Eugene Wong ◽  
Ian C. Francis
Keyword(s):  
Clinical Practice ◽  
Cataract Surgery ◽  
Exposure Time ◽  
Ocular Surface ◽  
Povidone Iodine ◽  
Bacterial Load ◽  
Minute Exposure ◽  
Visual Outcomes ◽  
Preoperative Prophylaxis ◽  
Operating Room Staff

Purpose Povidone-iodine (PI) is widely used to reduce the preoperative conjunctival bacterial load. This study aimed to evaluate the employment of PI 10% in an attempt to sterilize the ocular surface prior to cataract surgery, and to show that PI could be left in contact for 3 minutes. The viability of this exposure time in clinical practice, associated adverse events, and visual outcomes were documented. Methods In this prospective cohort study, phacoemulsification cataract surgery was performed in 604 patients by a single surgeon. Preoperative preparation was undertaken with PI 10%, applied to the cornea, conjunctival sac, eyelids, and periorbital skin with sterile cotton gauze. Povidone-iodine was then flushed onto the ocular surface. Operating room staff timed the precise duration of exposure. After the 3-minute preparation, the lids were thoroughly dried with fresh dry gauze. Results The median PI exposure time was 3.17 minutes, with an interquartile range of 0.25. All cases were followed up postoperatively at 1 day, 1 week, and 1 month. There were no complications attributable to PI. Visual outcomes were satisfactory. Conclusions Implementation of a preoperative prophylaxis protocol that used PI 10% with a 3-minute exposure time can be performed in clinical practice. The 3-minute exposure time had no adverse sequelae.

Comments to: Povidone-Iodine 3-Minute Exposure Time is Viable in Preparation for Cataract Surgery

2017 ◽  
Vol 27 (6) ◽  
pp. e187-e188
Author(s):  
Hiroyuki Shimada ◽  
Hiroyuki Nakashizuka ◽  
Andrzej Grzybowski
Keyword(s):  
Cataract Surgery ◽  
Exposure Time ◽  
Povidone Iodine ◽  
Minute Exposure

An Evaluation of Three Biological Indicator Systems in Flash Sterilization

1987 ◽  
Vol 8 (8) ◽  
pp. 311-316 ◽  
Author(s):  
Helen Rosen Kotilainen ◽  
Nelson M. Gantz
Keyword(s):  
Exposure Time ◽  
Color Change ◽  
Biological Indicators ◽  
Biological Indicator ◽  
The Other ◽  
Minute Exposure ◽  
Color Changes ◽  
Indicator Systems

AbstractAn evaluation of two flash-sterilization-specific biological indicators (BI) and a traditional spore strip indicator was performed to assess sensitivity and reliability as reflected in survive/kill ratios. The Bis tested included: 3M's Attest® #1261, Amsco's Proof Flash™, and Castle® Tec Test. Survival after “come-up” time alone, (0 exposure) and one-, two-, and three-minute exposures at 273°F in a gravity displacement sterilizer was measured by media color change or turbidity after incubation at 55°C. Each cycle was replicated three times on two separate days with six of each BI per run. Positive Bis were subcultured as necessary. Proof Flash presented technical difficulties due to incomplete or impossible crushing of media vials, unexpected media color changes, and evaporation of media. Tec Test was not sufficiently resistant as survivors were not detected at any exposure time. The Attest had 100% survival at zero and one-minute exposures and 94% survival after the two-minute exposure. No survivors were detected after the three-minute exposure. Although each institution should evaluate Bis for their own use independently, the data indicate that Attest #1261 monitored the three-minute flash cycles more satisfactorily than the other Bis tested.

scholarly journals The optimal time for surgery in women with serous ovarian cancer

2016 ◽  
Vol 59 (4) ◽  
pp. 223-232 ◽  
Author(s):  
Jocelyn M. Stewart ◽  
Alicia A. Tone ◽  
Haiyan Jiang ◽  
Marcus Q. Bernardini ◽  
Sarah Ferguson ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Optimal Time ◽  
Serous Ovarian Cancer

Taking ecto- and endocervical scrapings for the cytological study

2016 ◽  
Vol 3 (2) ◽  
pp. 92-96
Author(s):  
V. A Kaptilnyy
Keyword(s):  
Clinical Practice ◽  
Uterine Cervix ◽  
Cytological Study ◽  
Routine Clinical Practice ◽  
Cellular Material ◽  
Sample Technique ◽  
Cytological Method ◽  
Cytological Sample

In the article there is presented the technique of taking ecto- and endocervical scrapings for the cytological method of research. There are described in details the various techniques used in routine clinical practice. Particular attention is paid to the quality of the collecting cytological sample - technique of taking samples of cellular material. There is described the preparation to the study, readings and research goals. The accompanying photos illustrate in detail all the stages of the cytological screening of the uterine cervix.

Author's Reply to Comments to: Povidone-Iodine 3-Minute Exposure Time is Viable in Preparation for Cataract Surgery

2017 ◽  
Vol 27 (6) ◽  
pp. e189-e189 ◽  
Author(s):  
Chu L. Nguyen ◽  
Lawrence J. Oh ◽  
Eugene Wong ◽  
Ian C. Francis
Keyword(s):  
Cataract Surgery ◽  
Exposure Time ◽  
Povidone Iodine ◽  
Minute Exposure

Blood corticosteroids and anterior pituitary ACTH and cytology of dogs exposed to hypocapnia and/or hypoxemia

1959 ◽  
Vol 197 (1) ◽  
pp. 126-128 ◽  
Author(s):  
Clyde Biddulph ◽  
J. C. Finerty ◽  
J. P. Ellis
Keyword(s):  
Ascorbic Acid ◽  
Acid Content ◽  
Anterior Pituitary ◽  
Cytological Study ◽  
Venous Blood ◽  
Ascorbic Acid Content ◽  
Peripheral Venous Blood ◽  
Minute Exposure ◽  
Pituitary Tissue

The concentration of 17-hydroxycorticosterone and a corticosterone-like fraction in peripheral venous blood was not elevated significantly following 15- or 30-minute exposure of dogs to hypocapnia induced by hyperventilation, to hypoxemia induced by breathing 5% oxygen, or to the combination of these conditions. Neither were there significant differences between control and exposed animals in ACTH content of anterior pituitary tissue, or in ascorbic acid content of adrenals. Cytological study of sections of anterior pituitaries revealed somewhat greater than normal acidophilia in all dogs, with the greatest acidophilia being observed in the hypocapnic animals. The concentration of 17-hydroxycorticosterone was higher than that of the corticosterone-like fraction, the 17-hydroxycorticosterone to corticosterone-like ratio varying from 1.3:1 to 1.7:1.

Is it possible to define an optimal time for chemotherapy after surgery for ovarian cancer?

2014 ◽  
Vol 133 (3) ◽  
pp. 454-459 ◽  
Author(s):  
L. Lydiksen ◽  
S. Jensen-Fangel ◽  
J. Blaakaer
Keyword(s):  
Ovarian Cancer ◽  
Optimal Time
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