PERBANDINGAN PEMERIKSAAN TINJA METODE SEDIMENTASI DENGAN METODE NATIF DALAM MENDETEKSI SOIL TRANSMITTED HELMINTH

Soil Transmitted Helminth (STH) is the most common intestinal parasific infection and in one of the contributors to the global disease burden. Diagnosis of Soil Transmitted Helminth desease can be done using sedimentation method and natif method. This study aims to find out the differences in sedimentation methods and natif methods in deteting Soil Transmitted Helminth Type of research conducted ind true experimental form. This research was conducted on March 27-29, 2021 at the Microbiology Laboratory of Muhammadiyah Institute of Health and Technology Palembang which numbered 35 respondents. The results obtained in this study were as many as 2 positive samples and 33 negative samples. In sample 1 there are 2 types of eggs Soil Transmitted Helminth namely eggs Ascaris lumbricoides and Trichuris trichiura on sedimentation method and natif method, while in sample 5 there are eggs Ascaris lumbricoides only on the natif method. The results of the research data were conduted using an alternative test Wilcoxon with a value of P = 0.317 means there is no difference that signifikan.the conclusion of this study was obtained by the examination that there is no difference in sedimentation with natiif method.

Endoparasite infestation of Indian Muntjac (Muntiacus muntjak) in Citra Pesona Ladangku Animal Park, North Sumatra

An investigation on the infestation by endoparasites in the fecal samples of Indian Muntjac (Muntiacus muntjak) captived at Citra Pesona Ladangku Animal Park, North Sumatera, Indonesia had been conducted from October to November 2020. The study aimed to determine the endoparasite species, prevalence, and intensity of the infestation. Fecal samples were prepared for microscopical examination using glass bead sedimentation method. Based the observation on 9 deers, three species of endoparasites were found namely Ascaris sp, Haemonchus sp and Paramphistomum sp. Observations in the first week revealed the prevalence of each species from often (Ascaris sp, Haemonchus sp) to common (Paramphistomum sp) with light (Haemonchus sp, Paramphistomum sp) to moderate (Ascaris sp). Observations in the second week only revealed the presence of Paramphistomum with often prevalency and light intensity of infection.

Contamination of Soil Transmitted Helminths (STH) Eggs in Raw Vegetables at Street Food Stalls and Restaurant in Lorok Pakjo Village, Palembang

Background: Infection Soil Transmitted Helminths (STH) is a disease that is a problem in the world, especially in areas with a tropical climate like Indonesia. This infection can be transmitted through consumption of raw vegetables contaminated with STH. This study aims to determine whether there is contamination of STH eggs in raw vegetables at street food stalls and restaurants in Lorok Pakjo Village, Palembang. Methods: This research is a descriptive observational study. Samples in the form of fresh vegetables were collected from 18 street stalls and 14 restaurants in Lorok Pakjo Village, Palembang. Samples were examined using the sedimentation method and observed using a microscope. Results: The results obtained 80 samples consisting of 30 cabbage, 18 basil, 30 cucumber, 1 lettuce, and 1 eggplant. Of the 80 samples, there were 20 (25%) positive samples contaminated with STH eggs consisting of 8 (10%) cabbage samples, 8 (10%) basil samples, 4 (5%) cucumber samples, and no STH contamination was found in lettuce. and eggplant. Of the 20 positive samples of STH contamination, 12 samples came from street vendors consisting of 5 (10.6%) cabbage samples, 6 (12.8%) basil samples, and 1 (2.1%) cucumber samples. While the other 8 samples came from restaurants consisting of 3 (9.1%) cabbage samples, 2 (6%) basil samples, and 3 (9.1%) cucumber samples. Found only eggs of Ascaris lumbricoides infertile decorticate in all samples consisting of 9 eggs on cabbage, 16 eggs on basil, and five eggs on cucumber. Conclusion: There is contamination of eggs Soil Transmitted Helminths in raw vegetables in street food stalls and restaurants in Lorok Pakjo Village, Palembang.

Contamination of Soil Transmitted Helminths (STH) Eggs in Raw Vegetables at Street Food Stalls and Restaurant in Lorok Pakjo Village, Palembang

Background: Infection Soil Transmitted Helminths (STH) is a disease that is a problem in the world, especially in areas with a tropical climate like Indonesia. This infection can be transmitted through consumption of raw vegetables contaminated with STH. This study aims to determine whether there is contamination of STH eggs in raw vegetables at street food stalls and restaurants in Lorok Pakjo Village, Palembang. Methods: This research is a descriptive observational study. Samples in the form of fresh vegetables were collected from 18 street stalls and 14 restaurants in Lorok Pakjo Village, Palembang. Samples were examined using the sedimentation method and observed using a microscope. Results: The results obtained 80 samples consisting of 30 cabbage, 18 basil, 30 cucumber, 1 lettuce, and 1 eggplant. Of the 80 samples, there were 20 (25%) positive samples contaminated with STH eggs consisting of 8 (10%) cabbage samples, 8 (10%) basil samples, 4 (5%) cucumber samples, and no STH contamination was found in lettuce. and eggplant. Of the 20 positive samples of STH contamination, 12 samples came from street vendors consisting of 5 (10.6%) cabbage samples, 6 (12.8%) basil samples, and 1 (2.1%) cucumber samples. While the other 8 samples came from restaurants consisting of 3 (9.1%) cabbage samples, 2 (6%) basil samples, and 3 (9.1%) cucumber samples. Found only eggs of Ascaris lumbricoides infertile decorticate in all samples consisting of 9 eggs on cabbage, 16 eggs on basil, and five eggs on cucumber. Conclusion: There is contamination of eggs Soil Transmitted Helminths in raw vegetables in street food stalls and restaurants in Lorok Pakjo Village, Palembang.

Identification of "Soil Transmitted Helminth" Contamination on The Raw Vegetables in Warung Pecel Lele in Kebon Jeruk District, Jakarta

The prevalence of intestinal helminth infections in several provinces in Indonesia including Jakarta reaches 80% (or is still high). Helminth transmission may occur via food/drinks or through the skin. In particular, consumption of raw vegetables allows for the transmission of helminth eggs to happen. The purpose of this study was to identify intestinal worm eggs in raw vegetables sold in warung pecel lele. Raw vegetables were usually served together with the pecel lele menu which is a very popular dish among the Jakartans. The present study is a descriptive study with a cross sectional approach. Seventy-five samples of raw vegetables were included in this study, which were obtained from 25 warung pecel lele in Kebon Jeruk District, Jakarta. Samples were examined for helminth egg contamination based on a sedimentation method by lugol staining. Of all the raw vegetables samples, 20 samples (26.7%) was contaminated by soil transmitted helminths eggs. Ascaris lumbricoides worm eggs were identified in 17 samples (22.7%), whereas hookworm eggs were found in 3 samples (4%). The study suggests that the number of helminth egg contamination identified in raw vegetables are still high among warung pecel lele in West Jakarta.

Estimation of water holding capacity of soils using data from different types of particle size analyses

<p>Determining the particle size distribution of soils helps to monitor the hydrophysical properties of the soil (e.g. water conductivity or water holding capacity). Climate change increases the importance of water retention and permeability, as extreme weather events can severely impair the water supply of drought-prone plant stocks. The amount of water is expected to decrease. At the beginning of the research, we have developed a measurement method to replace the classical “pipette” sedimentation method with the laser diffraction method. The theoretical background of laser diffraction measurements is already known, but its practical application for estimating soil’s water holding capacity is uncommon in detail. The developed, modified Thornthwaite model considers soil properties (e.g. root depth, topsoil layer thickness) and size distribution (silt and clay fraction) of soil particles combined with the most significant soil properties. The pre-sieving of soil aggregates, the pre-treatment (disaggregation and dispersion) of the samples greatly influence the obtained results. In addition to the sedimentation method, instrumental measurements (Mastersizer 3000) were applied with three variants of pre-treatment. For comparison, the results of a Leptosol, a Cambisol, and a Luvisol were prepared for the first modified Thornthwaite water balance model. Significant differences appeared especially during drought periods that could be a basis for studying the drought sensitivity of soils. By the development of our method, the water holding capacity of soil can be estimated; therefore, adapting forest management could be planned against climatic and pedological transformations.</p>