scholarly journals Ante-mortem and post-mortem tuberculosis diagnostics in three European Bison from the enclosure in Bukowiec in the Bieszczady National Park in Poland

2017 ◽  
Vol 73 (10) ◽  
pp. 642-646 ◽  
Author(s):  
Krzysztof Anusz ◽  
Blanka Orłowska ◽  
Monika Krajewska-Wędzina ◽  
Ewa Augustynowicz-Kopeć ◽  
Michał Krzysiak ◽  
...  
Keyword(s):  
Bovine Tuberculosis ◽  
Lower Respiratory Tract ◽  
National Park ◽  
Complete Blood Count ◽  
Throat Swab ◽  
European Bison ◽  
Post Mortem ◽  
Free Living ◽  
Interferon Gamma Release Assays ◽  
Tuberculin Testing

The ante-mortem diagnostics of tuberculosis in wildlife constitutes a major problem due to the difficulties in collecting samples for examination. Therefore, tuberculosis in free-living animals is usually diagnosed post mortem. Ante-mortem examinations for bovine tuberculosis (BTB) were conducted in three European bison living in an acclimatisation enclosure in Bukowiec in the Bieszczady National Park in Poland. The reason for performing these examinations was the documented contact between the aforementioned animals and tuberculous European bison from a free-living herd present in the area. The three European bison were pharmacologically immobilised. Next, their blood samples were collected for immunological studies (interferon-gamma release assays), complete blood count and blood biochemistry tests. Moreover, bronchoalveolar lavage (BAL) fluid from the lower respiratory tract, two nasal cavity swabs and one throat swab were taken from each animal. Tuberculin testing was also performed. The findings of all these examinations indicated the possible infection of the European bison with the tubercle bacillus. Ultimately, the decision was taken to euthanize the animals.

scholarly journals Comparative Intradermal Tuberculin Testing of Free-Ranging African Buffaloes (Syncerus caffer) Captured forEx SituConservation in the Kafue Basin Ecosystem in Zambia

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Hetron Mweemba Munang'andu ◽  
Victor Siamudaala ◽  
Wigganson Matandiko ◽  
Andrew Nambota ◽  
John Bwalya Muma ◽  
...  
Keyword(s):  
National Parks ◽  
Bovine Tuberculosis ◽  
National Park ◽  
Tuberculin Test ◽  
Ex Situ ◽  
African Buffalo ◽  
Individual Animal ◽  
Syncerus Caffer ◽  
Tuberculin Testing ◽  
Free Ranging

Bovine tuberculosis (BTB) is endemic in African buffaloes (Syncerus caffer) in some National Parks in Southern Africa, whilst no studies have been conducted on BTB on buffalo populations in Zambia. The increased demand for ecotourism and conservation of the African buffalo on private owned game ranches has prompted the Zambian Wildlife Authority (ZAWA) and private sector in Zambia to generate a herd of “BTB-free buffaloes” forex situconservation. In the present study, 86 African buffaloes from four different herds comprising a total of 530 animals were investigated for the presence of BTB for the purpose of generating “BTB free” buffalo forex-situconservation. Using the comparative intradermal tuberculin test (CIDT) the BTB status at both individual animal and herd level was estimated to be 0.0% by the CIDT technique. Compared to Avian reactors only, a prevalence of 5.8% was determined whilst for Bovine-only reactors a prevalence of 0.0% was determined. These results suggest the likelihood of buffalo herds in the Kafue National Park being free of BTB.

scholarly journals A molecularly defined skin test reagent for the diagnosis of bovine tuberculosis compatible with vaccination against Johne’s Disease

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sonya Middleton ◽  
Sabine Steinbach ◽  
Michael Coad ◽  
Kevina McGill ◽  
Colm Brady ◽  
...  
Keyword(s):  
Skin Test ◽  
Recombinant Proteins ◽  
Bovine Tuberculosis ◽  
Synthetic Peptides ◽  
Blood Test ◽  
High Specificity ◽  
Active Components ◽  
Interferon Gamma Release Assays ◽  
Skin Responses

AbstractTuberculin Purified Protein Derivatives (PPDs) exhibit multiple limitations: they are crude extracts from mycobacterial cultures with largely unknown active components; their production depends on culture of mycobacteria requiring expensive BCL3 production facilities; and their potency depends on the technically demanding guinea pig assay. To overcome these limitations, we developed a molecularly defined tuberculin (MDT) by adding further antigens to our prototype reagent composed of ESAT-6, CFP-10 and Rv3615c (DIVA skin test, DST). In vitro screening using PBMC from infected and uninfected cattle shortlisted four antigens from a literature-based list of 18 to formulate the MDT. These four antigens plus the previously identified Rv3020c protein, produced as recombinant proteins or overlapping synthetic peptides, were formulated together with the three DST antigens into the MDT to test cattle experimentally and naturally infected with M. bovis, uninfected cattle and MAP vaccinated calves. We demonstrated significant increases in MDT-induced skin responses compared to DST in infected animals, whilst maintaining high specificity in unvaccinated or MAP vaccinated calves. Further, MDT can also be applied in in vitro blood-based interferon-gamma release assays. Thus, MDT promises to be a robust diagnostic skin and blood test reagent overcoming some of the limitations of PPDs and warrants full validation.

Noninvasive Tuberculosis Screening in Free-Living Primate Populations in Gombe National Park, Tanzania

EcoHealth ◽  
2015 ◽  
Vol 13 (1) ◽  
pp. 139-144 ◽  
Author(s):  
Tiffany M. Wolf ◽  
Srinand Sreevatsan ◽  
Randall S. Singer ◽  
Iddi Lipende ◽  
Anthony Collins ◽  
...  
Keyword(s):  
National Park ◽  
Free Living ◽  
Tuberculosis Screening ◽  
Gombe National Park

scholarly journals NECROBACILLOSIS IN FREE-LIVING MALE EUROPEAN BISON IN POLAND

2000 ◽  
Vol 36 (2) ◽  
pp. 248-256 ◽  
Author(s):  
Willi Jakob ◽  
Hans-Dieter Schröder ◽  
Michael Rudolph ◽  
Zbigniew A. Krasiński ◽  
Matgorzata Krasińska ◽  
...  
Keyword(s):  
European Bison ◽  
Free Living

scholarly journals Improvement of bovine tuberculosis diagnosis

2021 ◽  
pp. 261-265
Author(s):  
M. O. Baratov
Keyword(s):  
Red Blood Cells ◽  
Mycobacterium Bovis ◽  
Blood Cells ◽  
Bovine Tuberculosis ◽  
Intradermal Test ◽  
Mycobacterium Fortuitum ◽  
Post Mortem ◽  
Post Mortem Examination ◽  
Antibody Titers ◽  
Specific Reactions

Detection of animals with non-specific reactions to tuberculin is one of the major problems in bovine tuberculosis (TB) diagnosis. There is a need to find and improve methods for detection of the sensitization causes. This paper presents the results of comparative studies of different ways to stabilize red blood cells in order to obtain diagnosticums for indirect hemagglutination (IHA) test. The article describes the stages of red blood cells stabilization and sensitization and demonstrates the diagnostic significance of Fili stabilization method using formaldehyde as a fixative. The highest antibody titers (1:3000 and 1:4000) were received in hyperimmune sera of rabbits immunized with Mycobacterium bovis using a homologous diagnosticum. Practical importance of the sensitins homologous to the infection is shown during testing of 1,911 serum samples collected from animals of different categories (diseased; healthy and reacting to tuberculin; healthy and not reacting to tuberculin) with IHA test using diagnosticums produced from Mycobacterium bovis and Mycobacterium fortuitum. Based on the positive results of the IHA test, TB was diagnosed in 87.5% of animals originating from an infected farm during post-mortem examination. The results of the IHA test agreed with those of the intradermal tuberculin test in 37.7% of cases. Diagnostic antibody titers were found in 206 TB infected animals with no reaction to the intradermal test. However, the post-mortem examination revealed TB changes in internal organs. The obtained data suggest a possibility to use the IHA test to detect TB infected animals with non-specific reactions to tuberculin.

scholarly journals Bovine tuberculosis in one cattle herd in Ibadan in Nigeria

2012 ◽  
Vol 49 (No. 11) ◽  
pp. 406-412 ◽  
Author(s):  
S. I B Cadmus ◽  
N. N Atsanda ◽  
S. O Oni ◽  
E. E U Akang
Keyword(s):  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Molecular Typing ◽  
Human Population ◽  
Tuberculin Test ◽  
Cattle Herd ◽  
Post Mortem ◽  
Risk Of Infection ◽  
Cultural Isolation ◽  
Male Animal

Bovine tuberculosis was investigated in one private herd with 171 cattle after five cases were suspected to be tuberculous following post mortem examination. Using the intradermal comparative cervical tuberculin test 18 (10.5%) animals (ages from 2 to 12 years) were positive: 11 animals of N’dama breed and seven animals of White Fulani (i.e. Bunaji) breed; 17 female and one male animal. In all 11 randomly selected positive reactors, a spectrum of tuberculous lesions affecting the lungs, spleen, heart, liver, and the lymph nodes were observed. All the smear samples obtained were positive for acid-fast bacilli; cultural isolation confirmed the growth of mycobacteria on pyruvate-enriched Loewenstein-Jensen medium, which were identified by molecular typing to be Mycobacterium bovis. This study demonstrates widespread infection in this cattle herd and potential risk of infection for the human population with M. bovis.

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