Individual Feed Efficiency Monitoring of Charolaise Candidate Young Bulls in Relation to Feeding Behavior and Self-Performance Test Results

This study evaluated the effect of differences in residual feed intake (RFI) of Charolaise candidate young bulls on feeding behavior and self-performance test results. Bulls were classified into high and low RFI (H-RFI, L-RFI) groups. Bulls were fed in a HOKOFARM system to measure individual animal intake and behavior. L-RFI bulls had significantly lower feed intakes (p = 0.002) and higher gain to feed ratio (p = 0.001), lower intake per day/kg DM (dry matter) (p = 0.002) and lower intake g/body weight/day (p < 0.001). L-RFI animals had lower visits number per day (p = 0.02), but spent longer time per visit (p = 0.02), and tended to have higher intake g/visit (p = 0.06) on feeders. The correlation between RFI and DMI (dry matter intake)/bodyweight/day as well as intake per day/kg were large and positive. Back-loin length and rump length, and moreover muzzle width and frame, showed negative correlations with RFI value. However, bulls with better RFI values associated with lower legs score. Results reveal that RFI was shown beneficial correlations with economically relevant self-performance traits. Further investigations are needed to seek additional indicator traits that are predictive for RFI.

Individual animal geographies for the more-than-human city: Storying synanthropy and cynanthropy with urban coyotes

Recent efforts within geography to deconstruct anthropocentric readings of the urban and explore the city as ‘multispecies’ or ‘more-than-human’ face substantial methodological challenges. This paper contributes an empirical case study of human-coyote urban cohabitations in the Greater Toronto Area, Canada, using a ‘hybrid’ methodological approach to individual animal geographies. It builds on dialogues surrounding animals’ geographies that centre individual animal lifeworlds and experiences, exploring coyotes as subjects and actors who participate in the co-creation of shared urban worlds. A methodological approach based on collaboration and storying recounts the tales of two coyotes – Urban10 and Blondie – and their kin whose stories are gleaned by weaving together diverse social and ecological research tools, including: participant observation with Coyote Watch Canada, document review, semi-structured interviews, GPS collar data, field investigations, ethological observations, and trail cameras. The discussion details implications in terms of cynanthropy – ‘becoming-canid’ as methodology, delving into coyote lifeworlds using hybrid tools – as well as synanthropy – coyote synurbization and more-than-human urban belongings. Dwelling with Urban10 and Blondie in cynanthropic exploration makes visible opportunities for multispecies researchers to generate knowledge collaboratively with other-than-humans. Findings surrounding synanthropy highlight the practices involved in adapting to and participating, ecologically and socially, in life in the multispecies city. Overall, this paper advances efforts aimed at developing innovative and experimental hybrid methodologies for animal geographies, and theoretical discussions around re-storying the more-than-human city towards livable multispecies futures.

Circulating Metabolites Indicate Differences in High and Low Residual Feed Intake Holstein Dairy Cows

Selection for more feed efficient dairy cows is key to improving sustainability and profitability of dairy production; however, underlying mechanisms contributing to individual animal feed efficiency are not fully understood. The objective of this study was to identify circulating metabolites, and pathways associated with those metabolites, that differ between efficient and inefficient Holstein dairy cows using targeted metabolite quantification and untargeted metabolomics. The top and bottom fifteen percent of cows (n = 28/group) with the lowest and highest residual feed intake in mid-lactation feed efficiency trials were grouped retrospectively as high-efficient (HE) and low-efficient (LE). Blood samples were collected for quantification of energy metabolites, markers of hepatic function, and acylcarnitines, in addition to a broader investigation using untargeted metabolomics. Short-chain acylcarnitines, C3-acylcarnitine, and C4-acylcarntine were lower in HE cows (n = 18/group). Untargeted metabolomics and multivariate analysis identified thirty-nine differential metabolites between HE and LE (n = 8/group), of which twenty-five were lower and fourteen were higher in HE. Pathway enrichment analysis indicated differences in tryptophan metabolism. Combined results from targeted metabolite quantification and untargeted metabolomics indicate differences in fatty acid and amino acid metabolism between HE and LE cows. These differences may indicate post-absorptive nutrient use efficiency as a contributor to individual animal variation in feed efficiency.

Abomasal ulcers in cattle

Abomasal ulcers in cattle often present with non-specific clinical signs meaning that it is one of several differentials that may be relevant for a wide range of clinical presentations. This, combined with a lack of accurate diagnostic tests, makes the condition hard for the clinician to diagnose with confidence. The management and prognosis for cattle differ significantly depending on the severity and chronicity of an abomasal ulcer. It is important that clinicians are aware of the possibilities for diagnosis, treatment, and prognosis of the varying forms of this condition so they can offer the most appropriate management plans for the individual animal in every case.

186 Have New Feeding Technologies Revolutionized the Way We Design Grazing Experiments?

New technologies have enabled autonomous measurement of intake and feeding behavior of pen-fed animals and this has piqued interest in utilizing similar technologies in grazing experiments. Various feeding systems are currently available, but individual supplementation and feeding experiments have been conducted for years using individual feeding stanchions, which are the lowest level of technology for measuring supplement intake. These experiments are laborious as they require animals to be trained and acclimated, gathered at each feeding, and sorted into individual stalls for supplementation—thereby disrupting the animals grazing activities. In the mid-1980s Calan Gate systems (American Calan; Northwood, NH) became popular in pasture supplementation experiments, but still have many of the same disadvantages. Newer, more automated systems, reduce labor and possibly have less disruption of the animal’s grazing behavior, but are reliant on the animal’s willingness to use the system and ability to access it when more dominant peers are present. Individuals also do not always follow the “averages”-based rules we often hold true and behave differently in groups. Recent research with automatic feeders show steers consumed average of 60% of the potential supplement with supplement intake CVs of 50 to 100%. These technologies lead many to question the paradigm of experimental units in experimental designs. The experimental unit is defined as the smallest independently assigned unit to which the treatment is applied. This definition of experimental unit is somewhat limiting when taken to the extreme, but still applies well for experiments utilizing individual animal feeding, regardless of the level of technology involved. In many cases where the aforementioned technologies are used, the individual animal can be both the observational and experimental unit. This symposia presentation will discuss attributes of different feeding options and the implications on study design, experimental power, and the designated experimental unit.

Seroprevalence and Associated Risk Factors of Leptospira interrogans Serogroup Sejroe Serovar Hardjo in Dairy Farms in and around Jimma Town, Southwestern Ethiopia

A cross-sectional study was conducted on selected dairy farms in and around Jimma town, Oromia, southwestern Ethiopia from November 2019 to May 2020 to determine the seroprevalence of Leptospira interrogans serogroup Sejroe serovar Hardjo (L. hardjo). Furthermore, information was gathered on individual animal and herd level by using pretested semistructured questionnaire to assess associated risk factors. A stratified and simple random sampling procedure was used for the selection of dairy farms and individual animal’s, respectively. Indirect enzyme-linked immunosorbent assay (I-ELISA) was used in this study to detect antibody against L. hardjo. Out of 384 animal’s sera, 94 animals were seropositive against L. hardjo antibodies. From 77 dairy farms selected for the study, 57 of them were distinguished as positive for L. hardjo. The overall seroprevalence of leptospirosis caused by L. hardjo was 24.48% (95% CI: 20.18%–28.78%) and 74.03% (95% CI: 64.23%–83.82%) at individual animal and farm level, respectively. The result of multilogistic regression analysis revealed that management system ( p < 0.05; OR = 4.25 (95% CI: 2.31–7.82)), hygienic status of the farm ( p < 0.05; OR = 0.35 (95% CI: 0.20–0.61)), age of animals ( p < 0.05; OR = 8.30 (95% CI: 1.87–36.89)), history of abortion ( p < 0.05; OR = 8.37 (95% CI: 1.73–40.42)), herd size ( p < 0.05; OR = 2.32 (95% CI: 1.17–4.61)), and access of rodents to the farm ( p < 0.05; OR = 0.17 (95% CI: 0.03–0.86)) were significantly associated with the occurrence of L. hardjo infection. However, breed, parity, and introduction of new animals to the farm were insignificantly associated ( p > 0.05). Management system of the animal, hygienic status of the farm, herd size, age of animals, previous history of abortion, and access of rodents to the farm were identified as potential risk factors of L. hardjo disease occurrence. Thus, limiting rodents contact with cattle and their feed and water as well as good sanitary practices and husbandry management should be undertaken.

Both co-infection and superinfection drive complex Anaplasma marginale strain structure in a natural transmission setting

Vector-borne pathogens commonly establish multi-strain infections, also called complex infections. How complex infections are established, either prior to or after the development of an adaptive immune response, termed co-infection or superinfection, respectively, has broad implications for the maintenance of genetic diversity, pathogen phenotype, epidemiology, and disease control strategies. Anaplasma marginale , a genetically diverse, obligate, intracellular tick-borne bacterial pathogen of cattle commonly establishes complex infections, particularly in regions with high transmission rates. Both co-infection and superinfection can be established experimentally, however it is unknown how complex infections develop in a natural transmission setting. To address this question, we introduced naïve animals into a herd in southern Ghana with high infection prevalence and high transmission pressure and tracked strain acquisition of A. marginale through time using multi-locus sequence typing. As expected, genetic diversity among strains was high and 97% of animals in the herd harboured multiple strains. All the introduced, naïve animals became infected, and three to four strains were typically detected in an individual animal prior to seroconversion, while one to two new strains were detected in an individual animal following seroconversion. On average, the number of strains acquired via superinfection was 16% less than those acquired via co-infection. Thus, while complex infections develop via both co-infection and superinfection, co-infection predominates in this setting. These findings have broad implications for the development of control strategies in high transmission settings.

Evaluation of partial body weight for predicting body weight and average daily gain in growing beef cattle

Information on body weight and average daily gain (ADG) of growing animals is key not only to monitoring performance, but also for use in genetic evaluations in the pursuit of achieving sustainable genetic gain. Accurate calculation of ADG, however, requires serial measures of body weight over at least 70 days. This can be resource intensive and thus alternative approaches to predicting individual animal ADG warrant investigation. One such approach is the use of continuously collected individual animal partial body weights. The objective of the present study was to determine the utility of partial body weights in predicting both body weight and ADG; a secondary objective was to deduce the appropriate length of test to determine ADG from partial body weight records. The dataset used consisted of partial body weights, predicted body weights and recorded body weights recorded for 8,972 growing cattle from a range of different breed types in 35 contemporary groups. The relationships among partial body weight, predicted body weight and recorded body weight at the beginning and end of the performance test were determined and calculated ADG per animal from each body weight measure were also compared. On average, partial body weight explained 90.7 ± 2.0% of the variation in recorded body weight at the beginning of the postweaning gain test and 87.9 ± 2.9% of the variation in recorded body weight at its end. The GrowSafe proprietary algorithm to predict body weight from the partial body weight strengthened these coefficients of determination to 95.1 ± 0.9% and 94.9 ± 0.8%, respectively. The ADG calculated from the partial body weight or from the predicted body weight were very strongly correlated (r = 0.95); correlations between these ADG values with those calculated from the recorded body weights were weaker at 0.81 and 0.78, respectively. For some applications, ADG may be measured with sufficient accuracy with a test period of 50 days using partial body weights. The intended inference space is to individual trials which have been represented in this study by contemporary groups of growing cattle from different genotypes.