Abstract
The production of synergistically multi-acting cell wall hydrolyzing enzymes was observed in the culture filtrate of a strain molecularly characterized as Streptomyces rochei MZ227230. It showed high ability to produce chitinase, glucanase, protease and whole cell-wall lytic enzymes “WCL”. The simultaneous optimization production of several enzymes is a true challenge since each enzyme has its own favorable requirements that may not support or contradict the production of the others. Thus, the current study discussed such issue through application of statistical modeling. Productivity of the enzymes was monitored in different media and superiority of the modified TLE medium (M4) was clearly noticed. Box-Behnken design (BBD) using the key components of M4 (C. albicans cell walls, baker yeast, chitin and peptone) followed by independent analysis for productivity of each enzyme helped to conduct four significant models describing four media that were specifically optimized to produce the utmost yields of chitinase (14.97), glucanase (27.89), protease (137.59) and WCL (65.28 U/ml). Pearson’s correlation analysis indicated the impossibility to potentiate the tested enzymes to the utmost yields at the same time. However, simultaneous solution for all models’ equations identified a coproduction medium that potentiated more than 80% of the maximal yields for all enzymes. Finally, the crude filtrate showed potent fungicidal effects against Fusarium graminearum, Mucor racemosus, Fusarium solani, and Candida albicans which were proven to be attributed to the hydrolytic activity of the filtrate basing on dose and time-dependent release of C. albicans cell constituents DNA, RNA and protein as a model microbe.
Production of cell wall-hydrolyzing enzymes and GC-MS extract analysis of Byssochlamys lagunculariae, a new record isolated from Egypt
