Novel moisturized and antimicrobial hand gel based on zinc-aminoclay and Opuntia humifusa extract

The high antimicrobial ability and low toxicity of zinc-aminoclay (ZnAC) are claimed in our previous reports. In this study, we formulate a novel hand gel based on ZnAC and Opuntia humifusa (O. humifusa) extract, which is a high moisturizing agent. The antimicrobial activity, cytotoxicity, moisturizing effect, and clinical skin irritation of the hand gel are evaluated. The hand gel with 0.5 wt.% ZnAC and 1.0 v/v% O. humifusa extract can kill more than 99% Escherichia coli (gram-negative bacteria) and Staphylococcus aureus (gram-positive bacteria) after 24 h. Toxicity evaluation shows that, the hand gel does not affect the viability of mammalian HaCaT cells. Additionally, skin moisture is increased by applying the hand gel while its viscosity is at the standard level of commercial products. The hand gel has a skin irritation index of 0.0 and is classified as a non-irritating product. We successfully formulated hand gel from ZnAC, glucomannan, glycerol, and O. humifusa extract. Owing to the high antimicrobial activity and skin protection of hand gels, they are suitable to be used as hand sanitizers in restaurants, hospitals, and homes effectively.

Dispersal of Opuntia humifusa (Cactaceae) in the continental part of Ukraine

The localities of Opuntia humifusa (Cactaceae) in the continental part of Ukraine are described. All previous findings reported in Ukraine were made in the territory of the Autonomous Republic of Crimea, where the species sometimes behaves as a transformer and is threatening Crimea's natural phytodiversity. The first locality in continental Ukraine was discovered near Smila (Cherkasy Region) in a ruderal habitat; observations were conducted since 2015, the species increased the size of its population due to vegetative reproduction. The second locality was discovered in 2020 in Holosiivskyi National Nature Park (Kyiv), in a pine forest on a hill. There are also several localities, information about which is published in social networks and databases. Information of the range of the species is analyzed. Given the current understanding of taxonomy of the species, some records of its finds in Ukraine and in the world may belong to O. humifusa s. str. and, in some cases, to other species of the O. humifusa group. It is emphasized that O. humifusa can be expected in new localities. The species needs further monitoring of its distribution, especially in view of current climate changes that may promote its further spread.

Effect of a Synbiotic Containing Lactobacillus paracasei and Opuntia humifusa on a Murine Model of Irritable Bowel Syndrome

The administration of a combination of probiotics and prebiotics is expected to be a promising strategy for improving irritable bowel syndrome (IBS) symptoms. This study aimed to investigate the efficacy of a synbiotic containing Lactobacillus paracasei and Opuntia humifusa extract for symptomatic improvement of IBS in a murine model and to evaluate the mechanism underlying the beneficial effects of this synbiotic. A total of 20 male Wistar rats aged 8 weeks with IBS induced by restraint stress were assigned into four groups and administered L. paracasei as a probiotic and O. humifusa extract as a prebiotic for 4 weeks. The primary outcome was stool consistency at week 4. To evaluate the mechanism underlying the beneficial effects of the synbiotic, fecal microbial analysis was conducted, and the serum corticosterone levels, tumor necrosis factor-α (TNF-α) levels in the colon tissue, and expression of tight junction proteins were investigated. All three treatment groups showed significantly lower scores for stool consistency than the control group at week 4 (all p < 0.001). When compared with the control group, the synbiotic groups showed a significantly greater abundance of L. paracasei in fecal microbial analysis, lower serum corticosterone levels, lower TNF-α levels in the colon tissue, and higher expression of tight junction proteins. This novel synbiotic containing L. paracasei and O. humifusa extract can improve the stool consistency in a murine model of IBS. It may be a promising treatment option for IBS, and human studies are warranted.

Opuntia humifusa sensu stricto (Cactaceae): New to Kentucky (U.S.A.) and confirmed in the Unglaciated Appalachian Plateaus

A new population of Opuntia humifusa (Raf.) Raf. was found in Pike County, Kentucky. Consequent examination of other misidentified populations in Kentucky (Harlan and Pike counties) and West Virginia (Jackson County) provides the first known occurrences of Opuntia humifusa (sensu stricto) in Kentucky and a confirmed range extension into the Unglaciated Appalachian Plateaus Province.

Effects of Microwave-Assisted Opuntia humifusa Extract in Inhibiting the Impacts of Particulate Matter on Human Keratinocyte Skin Cell

Particulate matter (PM) is one of the most important factors causing serious skin diseases, due to its generation of reactive oxygen species (ROS) over the course of long-term exposure. As a source of natural antioxidants, Opuntia humifusa (O. humifusa) is a potential candidate for the design of advanced formulations to prevent PM’s harmful effects. Unfortunately, its high viscosity does not allow it to be utilized in these formulations. In this present study, a new approach to the extract of O. humifusa using high-power microwave treatment, namely microwave-assisted O. humifusa extract (MA-OHE), was investigated. The results indicated that MA-OHE not only is a reasonable viscosity extract, but also enhances O. humifusa’s antioxidant properties. Additionally, this study also found that MA-OHE exhibited outstanding antioxidant and anti-inflammatory activities in eliminating PM’s effects, due to suppression of AhR degradation, ROS production, and COX-2 and MMP-9 expression in HaCaT keratinocytes. It is believed that MA-OHE is a potential cosmeceutical ingredient that could be utilized to prevent PM-induced skin oxidative stress and inflammation.