scholarly journals INSECTICIDAL POTENCY OF NATIVE ENTOMOPATHOGENIC FUNGI ISOLATES AGAINST THE Galleria mellonella (LEPIDOPTERA: PYRALIDAE) AND Spodoptera littoralis (LEPIDOPTERA: NOCTUIDAE) Larvae.

2015 ◽  
Vol 6 (5) ◽  
pp. 727-738
Author(s):  
H. Aamer ◽  
F. Kassem ◽  
Soad Ahmed ◽  
E. Abdallah
Keyword(s):  
Entomopathogenic Fungi ◽  
Spodoptera Littoralis ◽  
Galleria Mellonella ◽  
Lepidoptera Pyralidae ◽  
Lepidoptera Noctuidae

scholarly journals Effet des extraits aqueux de Calotropis procera sur les principaux ravageurs du chou en culture au Sénégal

2020 ◽  
Vol 14 (5) ◽  
pp. 1600-1610
Author(s):  
Saliou Ngom ◽  
Toffène Diome ◽  
Bocar Diop ◽  
Mbacké Sembene
Keyword(s):  
Plutella Xylostella ◽  
Spodoptera Littoralis ◽  
Calotropis Procera ◽  
P Value ◽  
Aqueous Extracts ◽  
Hellula Undalis ◽  
Lepidoptera Pyralidae ◽  
Extraits Aqueux ◽  
Cabbage Pests ◽  
Lepidoptera Noctuidae

Au Sénégal, le chou est l’un des légumes les plus cultivés et consommés, de par son cycle relativement court (60-90 jours après repiquage) et sa possibilité d’être cultivé toute l’année. Cependant il est attaqué par plusieurs ravageurs tels que Plutella xylostella (L.) (Lepidoptera, Plutellidae), Hellula undalis (F.) (Lepidoptera, Pyralidae) et Spodoptera littoralis (B.) (Lepidoptera, Noctuidae). L’objectif de cette étude était de contribuer à la lutte contre ces ravageurs du chou en culture dans la zone des Niayes en utilisant les extraits aqueux à base de feuilles de Calotropis procera (Apocynaceae). L’étude a été menée sur 28 parcelles élémentaires dans la zone des Niayes (durant la période du 3 janvier au 22 mars 2019). Les formulations d’extrait de feuilles de C. procera utilisant comme solvant l’eau de robinet et l’eau de puits (ER et EP), appliquées à des temps différents le matin (ERM, EPM) et le soir (ERS, EPS) ont montré leurs efficacités sur les larves de P. xylostella et H. undalis. Par contre, les extraits ne sont pas efficaces sur S. littoralis (P-value= 0,672). En plus de leurs effets biocides, les extraits à base de feuilles de C. procera semblent accélérer la croissance des plants de chou. La nature du solvant et la période du traitement n’ont pas montré une différence significative.Mots clés : Biocide, Brassicassée, Niayes, lutte biologique English title: Aqueous extracts effect of Calotropis procera on the principal cabbage pests in culture in Senegal In Senegal, the cabbage is one of the most cultivated and consumed vegetables, from its relatively short cycle (60-90 days after road repair) and the possibility of being cultivated all the year. However it is attacked by several pests such as Plutella xylostella (L.) (Lepidoptera, Plutellidae), Hellula undalis (F.) (Lepidoptera, Pyralidae) and Spodoptera littoralis (B.) (Lepidoptera, Noctuidae). The objective of this study was to contribute on protect against cabbage pests in culture in the zone of Niayes by using the aqueous extracts containing sheets of Calotropis procera (Apocynaceae). The study was undertaken on 28 elementary pieces to in the zone of Niayes (during the period of January 3 at March 22, 2019). The formulations of extract using as solvent the water of tap and the water of well (ER and EP), applied to different times morning (ERM, EPM) and evening (ERS, EPS) showed their effectiveness on larvae of P. xylostella and H. undalis. On the other part S. littoralis was resistant to treatment (P=0,672) confirms it. In addition to their biocides effects, the extracts containing sheets of C procera seem accelerated the cabbage patches growth. The nature of solvent and the treatment period did not show a significant difference.Keywords: Biocide, Brassicasseae, Niayes, biological control

scholarly journals Photorhabdus luminescens subsp. akhurstii SL0708 pathogenicity in Spodoptera frugiperda (Lepidoptera: Noctuidae) and Galleria mellonella (Lepidoptera: Pyralidae)

2017 ◽  
Vol 20 (4) ◽  
pp. 1112-1121 ◽  
Author(s):  
Julián David Salazar-Gutiérrez ◽  
Andrés Castelblanco ◽  
María Ximena Rodríguez-Bocanegra ◽  
Wilson Teran ◽  
Adriana Sáenz-Aponte
Keyword(s):  
Spodoptera Frugiperda ◽  
Galleria Mellonella ◽  
Photorhabdus Luminescens ◽  
Lepidoptera Pyralidae ◽  
Lepidoptera Noctuidae

scholarly journals Soil drenching with entomopathogenic fungi for control of the soil-dwelling life stages and adults of the same generation of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae)

2019 ◽  
Vol 110 (2) ◽  
pp. 242-248 ◽  
Author(s):  
I. Garrido-Jurado ◽  
G. Resquín-Romero ◽  
M. Yousef-Naef ◽  
A. Ríos-Moreno ◽  
E. Quesada-Moraga
Keyword(s):  
Entomopathogenic Fungi ◽  
Spodoptera Littoralis ◽  
Reproductive Potential ◽  
Total Mortality ◽  
Life Stages ◽  
Egg Viability ◽  
Survival Times ◽  
Average Survival ◽  
Lepidoptera Noctuidae ◽  
Overall Mortality

AbstractFour Beauveria bassiana and three Metarhizium brunneum isolates were evaluated, as soil drenches, against Spodoptera littoralis prepupae. Treatment efficacy was determined by assessing total mortality during development from prepupae through to pupae and adults; mortality and sub-lethal effects on reproduction were also quantified for adults emerging from surviving prepupae/pupae. All isolates were pathogenic but overall mortality varied between 31.7 and 83.3% (0% for control); average survival time was 7.5–10.5 days (14.0 days for control). From 1.7–15.0% of adults emerging from surviving prepupae/pupae were deformed (0% in control). Contact with fungal suspensions as prepupae/pupae caused a significant reduction in fecundity of emerging adult females (15–58.9%), and a significant reduction in egg viability (6.8–28.4%) compared with controls. Two isolates were selected for virulence evaluation against S. littoralis prepupae. The LC50s were 1.7 × 107 and 1.8 × 107 conidia ml−1 and the median survival times were 7 and 6 days for isolates EAMa 01/58-Su and EAMb 09/01-Su, respectively. Destruxin A was present in pupae developing from prepupae treated with isolates EAMa 01/58-Su (0.010 ± 0.002 µg pupae−1) and EAMb 09/01-Su (0.015 ± 0.003 µg pupae−1). The use of entomopathogenic fungi as soil drenches could be a key component of S. littoralis IPM strategies due to direct reductions in the number of soil-dwelling life stages and, also, the significant reduction in reproductive potential of surviving adults.

scholarly journals Efficacy of the entomopathogenic nematode isolates against Spodoptera littoralis (Boisduval) and Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae)

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Gehan Mohamed Nouh
Keyword(s):  
Spodoptera Littoralis ◽  
Galleria Mellonella ◽  
Insect Pests ◽  
Entomopathogenic Nematode ◽  
Insect Pest ◽  
Soil Samples ◽  
Agrotis Ipsilon ◽  
Pest Species ◽  
Egyptian Clover ◽  
Lepidoptera Noctuidae

Abstract Background Entomopathogenic nematodes (EPNs), as biological control agents, have been isolated from many regions throughout the world. Local isolates of EPNs are usually more effective for controlling indigenous insect pests as they are adapted to the local environmental conditions and the insect pest species. Results In the present work, EPN isolates were searched in the soil under citrus and guava trees, and Egyptian clover at Noubaria region, Elbhaira governorate, Egypt, within two consecutive years. The EPNs were isolated from two positive soil samples of Egyptian clover (Trifolium alexandrinum) (TAN5) and guava trees (Psidium guajava) (PGN6), while the EPNs were not existent in the soil samples under citrus. Laboratory applications of the two EPNs isolates against the cotton leafworm, Spodoptera littoralis(Boisd.), and the black cutworm, Agrotis ipsilon (Hufn.) (Lepidoptera: Noctuidae), were recorded. Nematodes naturally occurring in the soil were trapped by full-grown larvae of the greater wax moth (Galleria mellonella L.). Infected larvae turned from whitish beige to dark reddish color, proofed that these isolates belong to the genus Heterorhabditis. Laboratory results revealed that the mortality rate ranges from 24 to 100% with TAN5 while from 18 to 96% with PGN6 at A. ipsilon larvae. The LC50 values of TAN5 against A. ipsilon were 1285.527 and 1560.747 IJs/cup, while those values for S. littoralis were 1339.099 and 2531.605 IJs/cup in larvae and pupae, respectively. The 3rd instar larvae of A. ipsilon and S. littoralis were more sensitive than the pupae. Production of Heterorhabditis sp. strain TAN5 was the highest in the reproduction of infective juveniles than the strain PGN6 at all concentrations. Conclusions The EPNs isolated from the soil samples belonged to the genus Heterorhabditis. Heterorhabditis sp. strain (TAN5) collected from the soil under Egyptian clover at Noubaria region was the highest reproduction and the most effective against both tested pests, A. ipsilon and S. littoralis larvae and pupae. EPN species would serve as an alternative to chemical pesticides and fit well in an integrated pest management program against larvae as well as adults and pupae of many economic insect pests which inhabit the soil.

scholarly journals Pathogenicity of some local entomopathogenic fungus isolates on the cotton leafworm larvae, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae)

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Funda Şahin ◽  
Yusuf Yanar
Keyword(s):  
Biological Control ◽  
Entomopathogenic Fungi ◽  
Entomopathogenic Fungus ◽  
Spodoptera Littoralis ◽  
Biological Control Agent ◽  
Mortality Rates ◽  
Control Agent ◽  
Soil Samples ◽  
Cotton Leaf ◽  
Lepidoptera Noctuidae

Abstract Background Pathogenicity of the entomopathogenic fungi (EPF), isolated from soil samples collected from Ordu Province, Turkey, was evaluated on the second-instar larvae of the cotton leaf worm Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) under laboratory conditions. Results Firstly, single-concentration response tests were conducted in order to determine the efficacy of the 64 isolates on S. littoralis larvae at the concentration of 1 × 108 conidia/ml. The five isolates displaying the highest mortality rates in single-concentration response tests, ORU-50, ORM-40, ORP-13, ORP-27 and ORM-48 (which included Beauveria bassiana, Metarhizium brunneum and Clonostachys rogersoniana), were subjected to concentration–response tests at the concentrations of 1 × 105–1 × 109 conidia/ml. The lowest LC50 and LC90 values were recorded at ORP-27 with 1.68 × 107 and 4.60 × 108 conidia/ml, respectively, followed by ORP-13 and ORM-40. Conclusions Accordingly, it was found that M. brunneum isolates were more effective than B. bassiana and C. rogersoniana against S. littoralis larvae. ORP-27, ORP-13 and ORM-40 of M. brunneum isolates can be a potential biological control agent used against S. littoralis larvae.

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