Genetic diversity studies on seven Egyptian wheat (Triticum aestivum) cultivars using Scot and ISSR polymorphism markers.

Saharan wheats have been studied particularly from a botanical viewpoint. Genotypic identification, classification and genetic diversity studies to date were essentially based on the morphology of the spike and grain. For this, the allelic variation at the glutenin loci was studied in a set of Saharan bread and durum wheats from Algerian oases where this crop has been traditionally cultivated. The high molecular weight and low molecular weight glutenin subunit composition of 40 Saharan bread and 30 durum wheats was determined by SDS-PAGE. In Saharan bread wheats 32 alleles at the six glutenin loci were detected, which in combination resulted in 36 different patterns including 17 for HMW and 23 for LMW glutenin subunits. For the Saharan durum wheats, 29 different alleles were identified for the five glutenin loci studied. Altogether, 29 glutenin patterns were detected, including 13 for HMW-GS and 20 for LMW-GS. Three new alleles were found in Saharan wheats, two in durum wheat at the Glu-B1 and Glu-B3 loci, and one in bread wheat at the Glu-B1 locus. The mean indices of genetic variation at the six loci in bread wheat and at the five loci in durum wheat were 0.59 and 0.63, respectively, showing that Saharan wheats were more diverse. This information could be useful to select Saharan varieties with improved quality and also as a source of genes to develop new lines when breeding for quality.

Download Full-text

Molecular diversity analysis in hexaploid wheat (Triticum aestivum L.) and two Aegilops species (Aegilops crassa and Aegilops cylindrica) using CBDP and SCoT markers

Journal of Genetic Engineering and Biotechnology ◽

10.1186/s43141-021-00157-8 ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Ghazal Ghobadi ◽

Alireza Etminan ◽

Ali Mehras Mehrabi ◽

Lia Shooshtari

Keyword(s):

Genetic Diversity ◽

Triticum Aestivum ◽

Gene Diversity ◽

Polymorphic Information Content ◽

Crop Improvement ◽

Wild Relatives ◽

Resolving Power ◽

Start Codon ◽

Aegilops Cylindrica ◽

Aegilops Crassa

Abstract Background Evaluation of genetic diversity and relationships among crop wild relatives is an important task in crop improvement. The main objective of the current study was to estimate molecular variability within the set of 91 samples from Triticum aestivum, Aegilops cylindrica, and Aegilops crassa species using 30 CAAT box–derived polymorphism (CBDP) and start codon targeted (SCoT) markers. Results Fifteen SCoT and Fifteen CBDP primers produced 262 and 298 fragments which all of them were polymorphic, respectively. The number of polymorphic bands (NPB), polymorphic information content (PIC), resolving power (Rp), and marker index (MI) for SCoT primers ranged from 14 to 23, 0.31 to 0.39, 2.55 to 7.49, and 7.56 to 14.46 with an average of 17.47, 0.34, 10.44, and 5.69, respectively, whereas these values for CBDP primers were 15 to 26, 0.28 to 0.36, 3.82 to 6.94, and 4.74 to 7.96 with a mean of 19.87, 0.31, 5.35, and 6.24, respectively. Based on both marker systems, analysis of molecular variance (AMOVA) indicated that the portion of genetic diversity within species was more than among them. In both analyses, the highest values of the number of observed (Na) and effective alleles (Ne), Nei’s gene diversity (He), and Shannon’s information index (I) were estimated for Ae. cylindrica species. Conclusion The results of cluster analysis and population structure showed that SCoT and CBDP markers grouped all samples based on their genomic constitutions. In conclusion, the used markers are very effective techniques for the evaluation of the genetic diversity in wild relatives of wheat.

Download Full-text

The new use of Sorghum bicolor-derived SSR markers to evaluate genetic diversity in 17 Australian Sorghum species

Plant Genetic Resources ◽

10.1079/pgr200454 ◽

2005 ◽

Vol 3 (1) ◽

pp. 19-28 ◽

Cited By ~ 12

Author(s):

Sally L. Dillon ◽

Peter K. Lawrence ◽

Robert J. Henry

Keyword(s):

Genetic Diversity ◽

Sorghum Bicolor ◽

Ssr Markers ◽

Diversity Indices ◽

Basic Knowledge ◽

Apparent Lack ◽

Breeding Programmes ◽

Diversity Studies ◽

Potential Use ◽

Simple Sequence

The Sorghum genus is extremely diverse both morphologically and geographically, however, relatively few of the 25 recognized species have been evaluated genetically. The apparent lack of basic knowledge pertaining to the levels of genetic diversity both within and between the 17 Australian wild species is a major obstacle to both their effective conservation and potential use in breeding programmes. Twelve Sorghum bicolor-derived simple sequence repeat (SSR) markers were evaluated for cross-species amplification in all 25 Sorghum species. The SSR markers were highly polymorphic, with diversity indices ranging from 0.59 to 0.99 with mean of 0.91. Five markers combined were able to differentiate 24 of the 25 Sorghum species, with intra-species polymorphism apparent. Sorghum bicolor-derived SSRs have proven to be an efficient source of markers for genetic diversity studies of the relatively poorly characterized Australian indigenous Sorghum species.

Download Full-text

Assessing genetic diversity in a set of wheat (Triticum aestivum) genotypes using microsatellite markers to improve the yellow rust resistant breeding programs

African Journal of Agricultural Research ◽

10.5897/ajar11.2059 ◽

2012 ◽

Vol 7 (48) ◽

pp. 6447-6455 ◽

Cited By ~ 1

Author(s):

Jamalirad S ◽

A Mohammadi S ◽

Toorchi M

Keyword(s):

Genetic Diversity ◽

Triticum Aestivum ◽

Microsatellite Markers ◽

Yellow Rust ◽

Breeding Programs

Download Full-text

Genetic Diversity and Geographical Differentiation in Asian Common Wheat (Triticum aestivum L.), Revealed by the Analysis of Peroxidase and Esterase Isozymes

Breeding Science ◽

10.1270/jsbbs.55.175 ◽

2005 ◽

Vol 55 (2) ◽

pp. 175-185 ◽

Cited By ~ 8

Author(s):

Surya Kant Ghimire ◽

Yukari Akashi ◽

Chieko Maitani ◽

Masakazu Nakanishi ◽

Kenji Kato

Keyword(s):

Genetic Diversity ◽

Triticum Aestivum ◽

Common Wheat ◽

Triticum Aestivum L ◽

Geographical Differentiation ◽

Esterase Isozymes

Download Full-text

Genetic Diversity Studies Based on Morphological Variability, Pathogenicity and Molecular Phylogeny of the Sclerotinia sclerotiorum Population From Indian Mustard (Brassica juncea)

Frontiers in Microbiology ◽

10.3389/fmicb.2018.01169 ◽

2018 ◽

Vol 9 ◽

Cited By ~ 7

Author(s):

Pankaj Sharma ◽

Amos Samkumar ◽

Mahesh Rao ◽

Vijay V. Singh ◽

Lakshman Prasad ◽

...

Keyword(s):

Genetic Diversity ◽

Molecular Phylogeny ◽

Brassica Juncea ◽

Sclerotinia Sclerotiorum ◽

Morphological Variability ◽

Indian Mustard ◽

Diversity Studies

Download Full-text

Review: Genetic diversity studies using microsatellite markers and their contribution in supporting sustainable sheep breeding programs

Asian Journal of Agriculture ◽

10.13057/asianjagric/g010109 ◽

2017 ◽

Vol 1 (01) ◽

pp. 46-51

Author(s):

OUMER SHERIFF ◽

KEFYALEW ALEMAYEHU

Keyword(s):

Genetic Diversity ◽

Microsatellite Markers ◽

Polymorphic Information Content ◽

Domestic Sheep ◽

Breeding Programs ◽

Sheep Breeding ◽

Population Structures ◽

The Mean ◽

Tree Building ◽

Diversity Studies

Sheriff O, Alemayehu K. 2017. Review: Genetic diversity studies using microsatellite markers and their contribution in supporting sustainable sheep breeding programs. Asian J Agric 1: 46-51. Microsatellites have been widely accepted and employed as useful molecular markers for measuring genetic diversity and divergence within and among populations. The various parameters developed so far to measure genetic diversity within and among populations are observed and expected heterozygosities (Ho and He), the mean number of alleles per locus (MNA),polymorphic information content (PIC), genetic distance and phylogenetic or tree building approach.The objective of thisreview was therefore to quantifythe genetic diversity studies of domestic sheep populations using microsatellite markersand their contribution in supporting sustainable sheep breeding programs. From the review, it is possible to see that there was high within population genetic variations in all the studied sheep populations, poor level of population differentiations and high levels of inbreeding. On the other hand, low estimates of hetrozygosities and mean number of alleles and employing only few and weak markers were observed in some of the studies. The gaps observed in the previous genetic diversity studies of the sheep populations may demand further works to reveal more information on the population structures andto start appropriate and sustainable breeding programs.

Download Full-text