The bioaugmentation of sequencing batch reactor sludges for biological phosphorus removal

1997 ◽  
Vol 35 (1) ◽  
pp. 19-26 ◽  
Author(s):  
E. Belia ◽  
P. G. Smith
Keyword(s):  
Activated Sludge ◽  
Nitrogen Removal ◽  
Pure Culture ◽  
Phosphorus Removal ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Sewage Treatment Plant ◽  
Biological Phosphorus Removal ◽  
Conventional Activated Sludge ◽  
Biological Phosphorus

The development of enhanced biological phosphorus removal (EBPR) through the bioaugmentation of a conventional activated sludge was studied. The objectives of the study were to evaluate the phosphorus removal capability of a sequencing batch reactor (SBR) when started with conventional activated sludge and augmented with a pure culture of Acinetobacter lwoffii. The effect of the addition of the pure culture on the reactor start up time, the settling properties of the sludge and on COD and nitrogen removal was also investigated. The effect of the removal of up to 70% of the bioaugmented biomass and its substitution with unconditioned sludge from a conventional sewage treatment plant was determined. This study has demonstrated that bioaugmentation can convert a conventional sewage works activated sludge to an EBPR sludge in 14 days. The sludge produced shows resilience to influent phosphate fluctuations, low D.O. and biomass replacement. The COD and nitrogen removal capabilities of the sludge and its settling properties are not affected by the addition of the pure culture.

pH: Keyfactor in the Biological Phosphorus Removal Process

1994 ◽  
Vol 29 (7) ◽  
pp. 71-74 ◽  
Author(s):  
G. J. F. Smolders ◽  
M. C. M. van Loosdrecht ◽  
J. J. Heijnen
Keyword(s):  
Activated Sludge ◽  
Phosphorus Removal ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Glycogen Metabolism ◽  
Activated Sludge Process ◽  
Biological Phosphorus Removal ◽  
Anaerobic Conditions ◽  
Biochemical Mechanisms ◽  
Biological Phosphorus

Experiments have been performed, using a sequencing batch reactor, to examine the effect of pH on biological phosphorus removal in the activated sludge process. The results, which indicate that glycogen metabolism occurs during anaerobic conditions, are useful in elucidating the biochemical mechanisms involved in phosphorus-removal, and have potential implications for systems such as Phostrip.

Simplicispira limi sp. nov., isolated from activated sludge

2007 ◽  
Vol 57 (1) ◽  
pp. 31-34 ◽  
Author(s):  
Shipeng Lu ◽  
Seung Hyun Ryu ◽  
Bok Sil Chung ◽  
Young Ryun Chung ◽  
Woojun Park ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Phosphorus Removal ◽  
Sequencing Batch Reactor ◽  
Novel Species ◽  
Batch Reactor ◽  
Molecular Characteristics ◽  
Rrna Gene ◽  
Biological Phosphorus Removal ◽  
16S Rrna Gene Sequences ◽  
Biological Phosphorus

A Gram-negative, motile, rod-shaped bacterium, designated strain EMB325T, was isolated from activated sludge that performed enhanced biological phosphorus removal in a sequencing batch reactor. The predominant fatty acids of strain EMB325T were summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2-OH), C16 : 0, C18 : 1 ω7c and C18 : 1 ω7c 11-methyl. The strain contained phosphatidylethanolamine and diphosphatidylglycerol as polar lipids. The G+C content of the genomic DNA was 63.3 mol%. The major quinone was Q-8. Phylogenetic analysis of 16S rRNA gene sequences showed that strain EMB325T formed a phyletic lineage with members of the genus Simplicispira and was most closely related to Simplicispira psychrophila LMG 5408T and Simplicispira metamorpha DSM 1837T with similarities of 98.1 and 97.9 %, respectively. Levels of DNA–DNA relatedness between strain EMB325T and S. psychrophila LMG 5408T and S. metamorpha DSM 1837T were 28 and 23 %, respectively. On the basis of chemotaxonomic data and molecular characteristics, strain EMB325T is considered to represent a novel species within the genus Simplicispira, for which the name Simplicispira limi sp. nov. is proposed. The type strain is EMB325T (=KCTC 12608T=DSM 17964T).

Bacteria and Protozoa Population Dynamics in Biological Phosphate Removal Systems

1994 ◽  
Vol 29 (7) ◽  
pp. 109-117 ◽  
Author(s):  
J. S. Čech ◽  
P. Hartman ◽  
M. Macek
Keyword(s):  
Population Dynamics ◽  
Phosphorus Removal ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Sole Source ◽  
Phosphate Removal ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
System Collapse ◽  
Biological Phosphorus

Population dynamics of polyphosphate-accumulating bacteria (PP bacteria) was studied in a laboratory sequencing batch reactor simulating anaerobic-oxic sludge system. The competition between PP bacteria and another microorganism (“G bacteria”) for anaerobic-oxic utilization of acetate as the sole source of organic carbon was observed. The competition was found to be seriously influenced by protozoan and metazoan grazing: Predation-resistant “G bacteria” forming large compact flocs outcompeted PP bacteria. Several breakdowns of enhanced biological phosphorus removal were observed. The first one was related to the development of an euglenid flagellate Entosiphon sulcatus and attached ciliates Vorticella microstoma and V. campanula. The second system collapse was connected with a rapid proliferation of rotifers. An alternative-prey predation was thought to be a mechanism of PP bacteria elimination.

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