Development and application of 16S rRNA-targeted oligonucleotide probe for detection of the phosphate-accumulating bacterium microlunatus phosphovorus in an enhanced biological phosphorus removal process

1998 ◽  
Vol 37 (4-5) ◽  
pp. 481-484 ◽  
Author(s):  
Mamoru Kawaharasaki ◽  
Takahiro Kanagawa ◽  
Hideo Tanaka ◽  
Kazunori Nakamura
Keyword(s):  
16S Rrna ◽  
Activated Sludge ◽  
Phosphorus Removal ◽  
Oligonucleotide Probe ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Phylogenetic Groups ◽  
Removal Process ◽  
Biological Phosphorus ◽  
Total Bacteria

A 16S rRNA-targeted oligonucletide probe (MP2) specific for the phosphate-accumulating bacterium (PAB) M. phosphovorus was designed and applied to a sludge from an enhanced biological phosphorus removal (EBPR) process. Probes specific for defined phylogenetic groups and the polyphosphate staining dye, DAPI (4′, 6-diamidino-2-phenylindol dihydrochloride) were also used to analyze the activated sludge community. M. phosphovorus was about 3% of the total bacteria in the EBPR sludge used. Proteobacteria belonging to the beta subclass were the most abundant. Many coccoid bacteria similar to M. phosphovorus were stained with DAPI. The percentage of PABs detected by DAPI stain was about 9% of the total bacteria.

Side-Stream EBPR Practices and Fundamentals – Rethinking and Reforming the Enhanced Biological Phosphorus Removal Process

2018 ◽  
Vol 2018 (5) ◽  
pp. 223-239
Author(s):  
Nicholas B Tooker ◽  
Guangyu Li ◽  
Varun Srinivasan ◽  
James L Barnard ◽  
Charles Bott ◽  
...  
Keyword(s):  
Phosphorus Removal ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Removal Process ◽  
Side Stream ◽  
Biological Phosphorus

Biochemical Aspects of Enhanced Biological Phosphorus Removal from Wastewater

1985 ◽  
Vol 17 (11-12) ◽  
pp. 23-41 ◽  
Author(s):  
M. C. Hascoet ◽  
M. Florentz ◽  
P. Granger
Keyword(s):  
Activated Sludge ◽  
Phosphorus Removal ◽  
Phosphorus Uptake ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
The Past ◽  
Nitrate Addition ◽  
The Subject ◽  
Biological Phosphorus ◽  
Microbiological Analyses

Enhanced biological phosphorus removal from wastewater by means of microorganisms found in activated sludge has for the past few years been the subject of much research and it is now commonly recognized that an activated sludge system must include alternating anaerobic-aerobic periods. The present article covers biochemical aspects of this phenomenon using a phosphorus removing biomass obtained in a laboratory-scale pilot with alternating phases and a synthetic substrate feed. The percentage of phosphorus obtained in the pilot sludge was four times greater than that of a conventional sludge plant. By exposing the same biomass to different conditions and using 31P Nuclear Magnetic Resonance, we were able to accurately pin-point the various forms of phosphorus found within cells and follow their development during the course of alternating phases. The following results were obtained:the transformation of phosphorus in its inorganic to polyphosphate form depends on the medium's level of oxygenation,the presence of nitrates disturbs the anaerobic period but does not affect phosphorus uptake in the aerated period.Continuous nitrate addition alters biomass behaviour in the anaerobic phase, which loses the capacity to release phosphorus,copper at a concentration of over 1 mg Cu2+/1 inhibits phosphorus uptake in the aerated phase. Various microbiological analyses made on the pilot biomass isolated conventional bacteria found in activated sludge.

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