Modelling and calibration of phosphate and glycogen accumulating organism competition for acetate uptake in a sequencing batch reactor

2004 ◽  
Vol 50 (6) ◽  
pp. 241-250 ◽  
Author(s):  
N. Yagci ◽  
G. Insel ◽  
N. Artan ◽  
D. Orhon
Keyword(s):  
Experimental Data ◽  
Mixed Culture ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Glycogen Metabolism ◽  
Numerical Results ◽  
Phosphate Removal ◽  
Proposed Model ◽  
New Process ◽  
And Performance

This paper improves the modeling of enhanced biological phosphate removal, by introducing the mechanistic description of a mixed culture of glycogen and phosphate accumulating organisms competing for acetate, and glycogen metabolism of the latter. A new process stoichiometry is defined for the mixed culture based upon previously developed metabolic concepts and models. The proposed model is tested with and used for the calibration of experimental data generated by a sequencing batch reactor operated with acetate feeding at a P/Ac ratio of 0.05 mgP mg-1COD. The calibration yielded acceptable numerical results for all the model coefficients tested and supported the validity of selected metabolic relationships for process stoichiometry. It also defined conditions that allow the system to sustain glycogen accumulating organisms with significant impact on process behaviour and performance.

Dynamics of phosphorus and organic substrates in anaerobic and aerobic phases of a sequencing batch reactor

1994 ◽  
Vol 30 (6) ◽  
pp. 237-246 ◽  
Author(s):  
A. Carucci ◽  
M. Majone ◽  
R. Ramadori ◽  
S. Rossetti
Keyword(s):  
Sequencing Batch Reactor ◽  
Municipal Wastewater ◽  
Batch Reactor ◽  
General Assumption ◽  
Organic Substrate ◽  
Operating Conditions ◽  
Phosphate Removal ◽  
Substrate Uptake ◽  
Organic Substrates ◽  
Polyphosphate Metabolism

This paper describes a lab-scale experimentation carried out to study enhanced biological phosphate removal (EBPR) in a sequencing batch reactor (SBR). The synthetic feed used was based on peptone and glucose as organic substrate to simulate the readily biodegradable fraction of a municipal wastewater (Wentzel et al., 1991). The experimental work was divided into two runs, each characterized by different operating conditions. The phosphorus removal efficiency was considerably higher in the absence of competition for organic substrate between P-accumulating and denitrifying bacteria. The activated sludge consisted mainly of peculiar microorganisms recently described by Cech and Hartman (1990) and called “G bacteria”. The results obtained seem to be inconsistent with the general assumption that the G bacteria are characterized by anaerobic substrate uptake not connected with any polyphosphate metabolism. Supplementary anaerobic batch tests utilizing glucose, peptone and acetate as organic substrates show that the role of acetate in the biochemical mechanisms promoting EBPR may not be so essential as it has been assumed till now.

scholarly journals Biohydrogen production under hyper salinity stress by an anaerobic sequencing batch reactor with mixed culture

2018 ◽  
Vol 16 (2) ◽  
pp. 159-170 ◽  
Author(s):  
Ensiyeh Taheri ◽  
Mohammad Mehdi Amin ◽  
Ali Fatehizadeh ◽  
Hamidreza Pourzamani ◽  
Bijan Bina ◽  
...  
Keyword(s):  
Mixed Culture ◽  
Salinity Stress ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Biohydrogen Production ◽  
Anaerobic Sequencing Batch Reactor

Initial Studies on the Anaerobic Sequencing Batch Reactor

1992 ◽  
Vol 26 (9-11) ◽  
pp. 2429-2432 ◽  
Author(s):  
R. R. Dague ◽  
C. E. Habben ◽  
S. R. Pidaparti
Keyword(s):  
Substrate Concentration ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
High Substrate ◽  
Anaerobic Sequencing Batch Reactor ◽  
New Process ◽  
Substrate Conversion ◽  
Solids Separation

This research focuses on an evaluation of the performance of a new process being called the “anaerobic sequencing batch reactor” (ASBR). The ASBR operates on an intermittent, fill and draw regimen. This results in alternating high substrate/low substrate (feast/famine) conditions. The high substrate conditions right after feeding results in high rates of substrate conversion to biogas. The low substrate concentration near the end of the react sequence results in efficient bioflocculation and solids separation.

Bacteria and Protozoa Population Dynamics in Biological Phosphate Removal Systems

1994 ◽  
Vol 29 (7) ◽  
pp. 109-117 ◽  
Author(s):  
J. S. Čech ◽  
P. Hartman ◽  
M. Macek
Keyword(s):  
Population Dynamics ◽  
Phosphorus Removal ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Sole Source ◽  
Phosphate Removal ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
System Collapse ◽  
Biological Phosphorus

Population dynamics of polyphosphate-accumulating bacteria (PP bacteria) was studied in a laboratory sequencing batch reactor simulating anaerobic-oxic sludge system. The competition between PP bacteria and another microorganism (“G bacteria”) for anaerobic-oxic utilization of acetate as the sole source of organic carbon was observed. The competition was found to be seriously influenced by protozoan and metazoan grazing: Predation-resistant “G bacteria” forming large compact flocs outcompeted PP bacteria. Several breakdowns of enhanced biological phosphorus removal were observed. The first one was related to the development of an euglenid flagellate Entosiphon sulcatus and attached ciliates Vorticella microstoma and V. campanula. The second system collapse was connected with a rapid proliferation of rotifers. An alternative-prey predation was thought to be a mechanism of PP bacteria elimination.

scholarly journals MODELING OF MIXED LIQUOR VOLATILE SUSPENDED SOLIDS AND PERFORMANCE EVALUATION FOR A SEQUENCING BATCH REACTOR

2015 ◽  
Vol 9 (1) ◽  
pp. 54-65
Author(s):  
Sayed Ahmad Mirbagheri ◽  
Majid Bagheri ◽  
Majid Ehteshami ◽  
Zahra Bagheri ◽  
Masoud Pourasghar
Keyword(s):  
Experimental Data ◽  
Retention Time ◽  
Sequencing Batch Reactor ◽  
Municipal Wastewater ◽  
Batch Reactor ◽  
Yield Coefficient ◽  
Cell Retention ◽  
Aeration Time ◽  
Test Models ◽  
Filling Time

This study examined carbon, nitrogen and phosphorous removal from municipal wastewater in a sequencing batch reactor and biokinetic coefficients were evaluated according to results of BOD and COD. Furthermore, the MLVSS in the aeration reactor was modeled by using multilayer perceptron and radial basis function artificial neural networks (MLPANN and RBFANN). The experiments were performed so that the cell retention time, filling time and intensity of aeration were (5, 10 and 15 d), (1, 2 and 3 h) and (weak, medium and strong) respectively. The result indicated that with cell retention time of 15 d, filling time of 1 h, aeration time of 6 h and settling time of 3 h the HRT is optimized at 10 h. The BOD5, COD, TP, TN and removal efficiencies were 97.13%, 94.58%, 94.27%, 89.7% and 92.75% respectively. The yield coefficient (Y), decay coefficient (Kd), maximum specific growth rate (K) and saturation constant (Ks) were 6.22 mgVSS/mgCOD, 0.002 1/d, 0.029 1/d and 20 mg COD/L according to COD experimental data. The values of the biokinetic coefficients were found to be as follows: Y = 10.45 mgVSS/mgBOD, Kd = 0.01 1/d, 0.014 1/d and 3.38 mgBOD/L according to BOD5 experimental data. The training procedures for simulation of MLVSS were highly collaborated for both RBFANN and MLPANN. The train and test models for both MLPANN and RBFANN demonstrated perfectly matched results between the experimental and the simulated values of MLVSS. The values of RMSE for train and test (verification) models obtained by MLPANN were 31.82 and 40.25 mg/L respectively, and the value of R2 was 0.99 for both models. The values of RMSE for train and test models obtained by MLPANN were 69.04 and 43.87 mg/L respectively, and the value of R2 was 0.99 for both models. It was observed that the MLPANN has stronger approximation and generalization ability than the RBFANN with regard to our experimental data for MLVSS.

scholarly journals MODELING OF MIXED LIQUOR VOLATILE SUSPENDED SOLIDS AND PERFORMANCE EVALUATION FOR A SEQUENCING BATCH REACTOR

2015 ◽  
Vol 9 (1) ◽  
pp. 54-65 ◽  
Author(s):  
Sayed Ahmad Mirbagheri ◽  
Majid Bagheri ◽  
Majid Ehteshami ◽  
Zahra Bagheri ◽  
Masoud Pourasghar
Keyword(s):  
Experimental Data ◽  
Retention Time ◽  
Sequencing Batch Reactor ◽  
Municipal Wastewater ◽  
Batch Reactor ◽  
Yield Coefficient ◽  
Cell Retention ◽  
Aeration Time ◽  
Test Models ◽  
Filling Time

This study examined carbon, nitrogen and phosphorous removal from municipal wastewater in a sequencing batch reactor and biokinetic coefficients were evaluated according to results of BOD and COD. Furthermore, the MLVSS in the aeration reactor was modeled by using multilayer perceptron and radial basis function artificial neural networks (MLPANN and RBFANN). The experiments were performed so that the cell retention time, filling time and intensity of aeration were (5, 10 and 15 d), (1, 2 and 3 h) and (weak, medium and strong) respectively. The result indicated that with cell retention time of 15 d, filling time of 1 h, aeration time of 6 h and settling time of 3 h the HRT is optimized at 10 h. The BOD5, COD, TP, TN and removal efficiencies were 97.13%, 94.58%, 94.27%, 89.7% and 92.75% respectively. The yield coefficient (Y), decay coefficient (Kd), maximum specific growth rate (K) and saturation constant (Ks) were 6.22 mgVSS/mgCOD, 0.002 1/d, 0.029 1/d and 20 mg COD/L according to COD experimental data. The values of the biokinetic coefficients were found to be as follows: Y = 10.45 mgVSS/mgBOD, Kd = 0.01 1/d, 0.014 1/d and 3.38 mgBOD/L according to BOD5 experimental data. The training procedures for simulation of MLVSS were highly collaborated for both RBFANN and MLPANN. The train and test models for both MLPANN and RBFANN demonstrated perfectly matched results between the experimental and the simulated values of MLVSS. The values of RMSE for train and test (verification) models obtained by MLPANN were 31.82 and 40.25 mg/L respectively, and the value of R2 was 0.99 for both models. The values of RMSE for train and test models obtained by MLPANN were 69.04 and 43.87 mg/L respectively, and the value of R2 was 0.99 for both models. It was observed that the MLPANN has stronger approximation and generalization ability than the RBFANN with regard to our experimental data for MLVSS.

scholarly journals Microbial community and performance of a partial nitritation/anammox sequencing batch reactor treating textile wastewater

Heliyon ◽  
2021 ◽  
pp. e08445
Author(s):  
Elisa Clagnan ◽  
Lorenzo Brusetti ◽  
Silvia Pioli ◽  
Simone Visigalli ◽  
Andrea Turolla ◽  
...  
Keyword(s):  
Microbial Community ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Textile Wastewater ◽  
Partial Nitritation ◽  
And Performance

Anaerobic uptake of phosphate in an anaerobic–aerobic granular sludge sequencing batch reactor

2006 ◽  
Vol 53 (9) ◽  
pp. 63-70 ◽  
Author(s):  
X. Wang ◽  
M. Ji ◽  
J.F. Wang ◽  
Z. Liu ◽  
Z.Y. Yang
Keyword(s):  
Sequencing Batch Reactor ◽  
Phosphate Uptake ◽  
Batch Reactor ◽  
Aerobic Condition ◽  
Granular Sludge ◽  
Organic Substrate ◽  
Phosphate Removal ◽  
Aerobic Granular Sludge ◽  
Unusual Phenomenon ◽  
Phosphate Carrier

An unusual phenomenon of anaerobic phosphate uptake under alternating anaerobic/aerobic condition was observed in a granular sludge sequencing batch reactor, fed with acetate as sole organic substrate. Anaerobic phosphate uptake efficiencies remained at 50–70% as the influent P/COD was increased from 2/100 to 4/100, and results showed that anaerobic uptake of phosphate was correlated with anaerobic absorption of acetate. Excluding the main possibility of chemical phosphate removal, it appeared that phosphate uptake during the anaerobic phase was associated with organisms enriched in the reactor. Moreover, results indicated that intracellular glycogen was used as the main energy source of organics anaerobic absorption and intracellular polymers storage. Measuring and analysing the variation of phosphate, organic substrate, intracellular glycogen and pH in the anaerobic phase, a preliminary explanation was developed that anaerobic uptake of phosphate was the demand of intracellular glycogen degradation, and extracellular phosphate was transported to intracellular by pH gradient-sensitive phosphate carrier protein.

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