A simple, rapid, accurate and economical method has been developed for the quantification of droxidopa by UPLC. The chromatographic system was equipped with Phenomenex column C18 (50mm x 3.0mm, 3µ) as stationary phase and UV detector at 235nm, in conjunction with a mobile phase of buffer: methanol (25:75 % v/v ratio) at a flow rate of 1.0mL/min. Linearity was observed over the concentration range of 100-300µg/mL for droxidopa. The droxidopa peak eluted at 0.35 min. The recovery of Droxidopa was found to be 100.17% - 100.63% respectively. Statistical techniques were employed for the validation of precision, linearity, accuracy, robustness and ruggedness and can be applied for routine analysis. Validation revealed that the developed method was specific, accurate, precise, reliable, robust, reproducible and suitable for the routine quantitative analysis.
Quantitative analysis of concrete using portable x-ray fluorescence: Method development and validation
