Immunotoxicity, Flow Cytometry, and Chemoinformatics: Review, Bibliometric Analysis, and New QSAR Model of Drug Effects Over Macrophages

The dependence of spermatogenesis function on murine age shall be investigated. Thus, testicular samples of at least 10 NMRI mice per group aged 0 to 26 months are analysed by flow cytometry after staining the DNA with DAPI. The aim of this study is to be able to account for the influence of age on mice. There are no changes in spermatogenesis in mice aged 11 weeks up to 16 months with respect to testis weight and to the frequency of different testicular cell types. From 16 months onwards, there is a tendency to a reduced spermatogenesis function; The frequency of round spermatids is decreased. In addition, there is an increased chromatin dispersion in elongated spermatids with age. Thus, older mice (>16 months) should not be used for experiments e.g. on radiation and drug effects any more. The frequency of abnormal diploid spermatozoa does not increase with age.

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Drug effects in multiple rounds of cell division monitored by flow cytometry

Journal of Inflammation ◽

10.1186/1476-9255-12-s1-o6 ◽

2015 ◽

Vol 12 (Suppl 1) ◽

pp. O6

Author(s):

Derek Davies

Keyword(s):

Flow Cytometry ◽

Cell Division ◽

Drug Effects

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Automation of cytokine flow cytometry assays

JALA Journal of the Association for Laboratory Automation ◽

10.1016/s1535-5535(03)00105-9 ◽

2004 ◽

Vol 9 (1) ◽

pp. 5-9 ◽

Cited By ~ 5

Author(s):

J DUNNE ◽

H MAECKER

Keyword(s):

Flow Cytometry ◽

Cytokine Flow Cytometry

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Intraepithelial lymphocytes and coeliac disease: permanent changes in CD3-/CD7+ and T cell receptor ?-d subsets studied by flow cytometry

Acta Paediatrica ◽

10.1080/080352500750028410 ◽

2000 ◽

Vol 89 (3) ◽

pp. 285-290 ◽

Cited By ~ 16

Author(s):

C. Camarero

Keyword(s):

Flow Cytometry ◽

T Cell ◽

Coeliac Disease ◽

T Cell Receptor ◽

Cell Receptor ◽

Intraepithelial Lymphocytes

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Review for "Diet‐induced microbial autofluorescence confounds flow cytometry of ex vivo isolated fecal microbes"

10.1002/eji.201948087/v1/review2 ◽

2019 ◽

Author(s):

Hyun-Dong Chang

Keyword(s):

Flow Cytometry ◽

Ex Vivo

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Reduction in Pain Sensitivity from Pharmacological Elevation of Blood Pressure in Persons with Chronically Low Blood Pressure

Journal of Psychophysiology ◽

10.1027/0269-8803.23.3.104 ◽

2009 ◽

Vol 23 (3) ◽

pp. 104-112 ◽

Cited By ~ 12

Author(s):

Stefan Duschek ◽

Heike Heiss ◽

Boriana Buechner ◽

Rainer Schandry

Keyword(s):

Blood Pressure ◽

Heart Rate ◽

Pain Sensitivity ◽

Pain Threshold ◽

Pain Perception ◽

Drug Effects ◽

Pressure Effects ◽

Double Blind ◽

Low Blood Pressure ◽

Present Trial

Recent studies have revealed evidence for increased pain sensitivity in individuals with chronically low blood pressure. The present trial explored whether pain sensitivity can be reduced by pharmacological elevation of blood pressure. Effects of the sympathomimetic midodrine on threshold and tolerance to heat pain were examined in 52 hypotensive persons (mean blood pressure 96/61 mmHg) based on a randomized, placebo-controlled, double-blind design. Heat stimuli were applied to the forearm via a contact thermode. Confounding of drug effects on pain perception with changes in skin temperature, temperature sensitivity, and mood were statistically controlled for. Compared to placebo, higher pain threshold and tolerance, increased blood pressure, as well as reduced heart rate were observed under the sympathomimetic condition. Increases in systolic blood pressure between points of measurement correlated positively with increases in pain threshold and tolerance, and decreases in heart rate were associated with increases in pain threshold. The findings underline the causal role of hypotension in the augmented pain sensitivity related to this condition. Pain reduction as a function of heart rate decrease suggests involvement of a baroreceptor-related mechanism in the pain attrition. The increased proneness of persons with chronic hypotension toward clinical pain is discussed.

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