Differential Proteomic Identification and Bioinformatics Analysis of Femoral Neck in Elderly Female Patients with Hyperuricaemia

2021 ◽  
Vol 18 ◽  
Author(s):  
Yingyi He ◽  
Guangming Zhang ◽  
Yuyang Huang ◽  
Qi Li ◽  
Cheng Luo
Keyword(s):  
Femoral Neck ◽  
Femoral Neck Fracture ◽  
Differentially Expressed ◽  
Control Group ◽  
Pathophysiological Mechanism ◽  
Differentially Expressed Proteins ◽  
Mineral Density ◽  
Differentially Expressed Protein ◽  
Peptide Mass ◽  
Neck Fracture

Background: Serum uric acid (UA) is positively correlated with bone mineral density (BMD). However, the mechanism by which serum UA affects BMD remains unclear. Objective: The aim was carried out to search for the functional proteins related to serum UA and femoral neck BMD to better understand the pathophysiological mechanism of osteoporosis. Materials and methods: In this study, patients in the UA group (hyperuricaemia combined with femoral neck fracture) and the control group (normal uricaemia combined with femoral neck fracture) were selected according to the inclusion criteria. Total protein was extracted from the femoral neck of each patient. Fluorescence differential gel electrophoresis was used to separate the total proteins, and the differentially expressed protein spots were detected by image analysis. After enzyme digestion, peptide mass fingerprinting and database searches were performed to identify the differentially expressed proteins. DAVID software and Kyoto Encyclopedia of Genes and Genomes (KEGG) data were used for enrichment analysis of the screened differential proteins. Results: After mass spectrometry and database searching, 66 differentially expressed protein spots were identified between the UA group and the control group. Most differentially expressed proteins functioned in cytoskeleton formation, energy metabolism, or signal transduction. They were mainly involved in 50 biological processes, including peroxisome proliferator-activated receptor (PPAR) signalling and fatty acid metabolism. PPARγ and PLIN1 were subject to Western blotting analysis detection; results were consistent with the Label-Free result. Conclusion: Based on an analysis of the biological information, these proteins may be associated with the incidence and progression of the femoral neck bone tissues of hyperuricaemia patients.

scholarly journals Treatment of Pauwels type III femoral neck fracture with medial femoral neck support screw: a biomechanical and clinical study

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zhichao Gao ◽  
Mei Wang ◽  
Baojie Shen ◽  
Xiaodong Chu ◽  
Di Ruan
Keyword(s):  
Femoral Head ◽  
Femoral Neck ◽  
Fracture Healing ◽  
Femoral Neck Fracture ◽  
Biomechanical Study ◽  
Femoral Head Necrosis ◽  
Vertical Shear ◽  
Control Group ◽  
Fixation Failure ◽  
Neck Fracture

AbstractA femoral neck fracture is currently one of the most common types of fracture in clinical practice. The incidence continues to increase due to traffic accidents, trauma, and osteoporosis. This research includes a biomechanical study and a clinical retrospective study. In the biomechanical studies, three groups’ effects (Control Group: 3CCS, DHS group, and study Group: 3CCS + mFNSS group) were compared by vertical compression tests, torsion tests, and fatigue tests. All the data were collected and analyzed. We subsequently performed a retrospective analysis of 131 patients with femoral neck fractures. The operative time, intraoperative blood loss, quality of postoperative fracture reduction, and follow-up observation of fracture healing, screw retreatment rates and fixation failure rates, as well as femoral head necrosis rates and hip function in two groups with 3CCS and 3CCS + mFNSS were compared. By the biomechanical study, we found that 3CCS + Mfnss group were biomechanically superior to 3CCS group and superior to the DHS group in terms of resistance to torsion. However, it was less effective than the DHS group in compressive strength and fatigue resistance. In terms of clinical application, 3CCS + mFNSS group was found to have lower screw retreatment rates and femoral head necrosis rates, and to have better fracture healing rates than group with 3CCS, indicating that medial support screws can effectively resist the vertical shear forces of fracture ends and promote the stability and healing of fracture ends, as well as to reduce the incidence of postoperative complications.

Effect of dicarboxymethyl trisodium alginate on the expression of BMP-7 protein in bone tissues of rats with traumatic femoral neck fracture

2016 ◽  
Author(s):  
Zhao Qin ◽  
LiPing Guan
Keyword(s):  
Femoral Neck ◽  
Femoral Neck Fracture ◽  
Sodium Alginate ◽  
Bone Cells ◽  
Medical Intervention ◽  
Control Group ◽  
Mrna Levels ◽  
Skeletal Growth Factor ◽  
Neck Fracture ◽  
Aldehyde Groups

Bone morphogenetic protein-7 (BMP-7) is a pivotal skeletal growth factor. Sodium alginate (SA) is a natural polysaccharide polymer extracted from brown alga. Partial uronic acid units of sodium alginate can be transformed into aldehyde groups, namely dicarboxymethyl trisodium alginate (DCMTSA). In this investigation, rats with traumatic femoral neck fractures were selected. The rats treated with dicarboxymethyl trisodium alginate intervention were assigned to the experimental group, those treated with sodium alginate intervention were in the control group, and those receiving no medical intervention were in the placebo group. The expression of BMP-7 at both the protein and mRNA levels in the rat fracture tissues in all three groups was quantitatively detected by immunohistochemicals SP and RT-PCR. Results revealed that both dicarboxymethyl trisodium alginate and sodium alginate induced the up-regulation of BMP-7 expression. However, the effect of dicarboxymethyl trisodium alginate was superior to that of sodium alginate. Therefore, dicarboxymethyl trisodium alginate can be used to promote the release of BMP-7 from bone cells and contribute to the bone union in traumatic femoral neck fracture in rat models.

scholarly journals Application of Robot Positioning for Cannulated Screw Internal Fixation in the Treatment of Femoral Neck Fracture: Retrospective Study (Preprint)

2020 ◽  
Author(s):  
Lei Wan ◽  
Xiangyun Zhang ◽  
Dalong Wu ◽  
Zhihao Li ◽  
Dongtao Yuan ◽  
...  
Keyword(s):  
Surgical Treatment ◽  
Femoral Neck ◽  
Internal Fixation ◽  
Fracture Healing ◽  
Femoral Neck Fracture ◽  
Cannulated Screw ◽  
Control Group ◽  
Study Group ◽  
Robot Assisted ◽  
Neck Fracture

BACKGROUND Femoral neck fracture is a common type of hip fracture. Conventional surgical treatment aims at fixing the fracture site with screws and then gradually promoting bone healing. A robot-assisted orthopedic surgery system is computer technology applied to surgical treatment. OBJECTIVE This study aimed to explore the therapeutic effect and prognostic value of percutaneous cannulated screw internal fixation using robot-assisted positioning in patients with femoral neck fractures. METHODS From July 2018 to September 2019, 42 cases of femoral neck fracture admitted to the Second Affiliated Hospital of Luohe Medical College were randomly and averagely divided into control and study groups. The patients in the control group were treated with conventional percutaneous cannulated screw internal fixation, while the patients in the study group were treated with robot-assisted percutaneous cannulated screw fixation during surgical treatment. We compared the treatment conditions and results of the operation between the 2 groups. The Harris score was used to evaluate the treatment efficacy. The state of fracture healing was followed up and compared between the 2 groups. RESULTS The duration of the operation was shorter, there was less fluoroscopy use, and there were fewer drilled holes in the study group than in the control group (all, <i>P</i>&lt;.001). There was no statistical difference in the amount of intraoperative bleeding between the 2 groups (<i>P</i>=.33). The Harris score (<i>P</i>=.045) and number of excellent and good ratings (<i>P</i>=.01) were significantly higher in the study group than in the control group. The difference in the fracture healing rate between the 2 groups was not statistically significant (<i>P</i>=.23). The fracture healing duration of the study group was shorter than that of the control group (<i>P</i>=.001). CONCLUSIONS The use of robotic positioning aids in the treatment of femoral neck fractures with percutaneous cannulated screw fixation can effectively improve the efficiency of surgery, shorten the duration of surgery, and reduce the radiation damage to patients. Meanwhile, it improves postoperative treatment and recovery rates of the patients and shortens the fracture healing time.

scholarly journals Application of Robot Positioning for Cannulated Screw Internal Fixation in the Treatment of Femoral Neck Fracture: Retrospective Study

10.2196/24164 ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. e24164
Author(s):  
Lei Wan ◽  
Xiangyun Zhang ◽  
Dalong Wu ◽  
Zhihao Li ◽  
Dongtao Yuan ◽  
...  
Keyword(s):  
Surgical Treatment ◽  
Femoral Neck ◽  
Internal Fixation ◽  
Fracture Healing ◽  
Femoral Neck Fracture ◽  
Cannulated Screw ◽  
Control Group ◽  
Study Group ◽  
Robot Assisted ◽  
Neck Fracture

Background Femoral neck fracture is a common type of hip fracture. Conventional surgical treatment aims at fixing the fracture site with screws and then gradually promoting bone healing. A robot-assisted orthopedic surgery system is computer technology applied to surgical treatment. Objective This study aimed to explore the therapeutic effect and prognostic value of percutaneous cannulated screw internal fixation using robot-assisted positioning in patients with femoral neck fractures. Methods From July 2018 to September 2019, 42 cases of femoral neck fracture admitted to the Second Affiliated Hospital of Luohe Medical College were randomly and averagely divided into control and study groups. The patients in the control group were treated with conventional percutaneous cannulated screw internal fixation, while the patients in the study group were treated with robot-assisted percutaneous cannulated screw fixation during surgical treatment. We compared the treatment conditions and results of the operation between the 2 groups. The Harris score was used to evaluate the treatment efficacy. The state of fracture healing was followed up and compared between the 2 groups. Results The duration of the operation was shorter, there was less fluoroscopy use, and there were fewer drilled holes in the study group than in the control group (all, P<.001). There was no statistical difference in the amount of intraoperative bleeding between the 2 groups (P=.33). The Harris score (P=.045) and number of excellent and good ratings (P=.01) were significantly higher in the study group than in the control group. The difference in the fracture healing rate between the 2 groups was not statistically significant (P=.23). The fracture healing duration of the study group was shorter than that of the control group (P=.001). Conclusions The use of robotic positioning aids in the treatment of femoral neck fractures with percutaneous cannulated screw fixation can effectively improve the efficiency of surgery, shorten the duration of surgery, and reduce the radiation damage to patients. Meanwhile, it improves postoperative treatment and recovery rates of the patients and shortens the fracture healing time.

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