Effect of dicarboxymethyl trisodium alginate on the expression of BMP-7 protein in bone tissues of rats with traumatic femoral neck fracture

2016 ◽  
Author(s):  
Zhao Qin ◽  
LiPing Guan
Keyword(s):  
Femoral Neck ◽  
Femoral Neck Fracture ◽  
Sodium Alginate ◽  
Bone Cells ◽  
Medical Intervention ◽  
Control Group ◽  
Mrna Levels ◽  
Skeletal Growth Factor ◽  
Neck Fracture ◽  
Aldehyde Groups

Bone morphogenetic protein-7 (BMP-7) is a pivotal skeletal growth factor. Sodium alginate (SA) is a natural polysaccharide polymer extracted from brown alga. Partial uronic acid units of sodium alginate can be transformed into aldehyde groups, namely dicarboxymethyl trisodium alginate (DCMTSA). In this investigation, rats with traumatic femoral neck fractures were selected. The rats treated with dicarboxymethyl trisodium alginate intervention were assigned to the experimental group, those treated with sodium alginate intervention were in the control group, and those receiving no medical intervention were in the placebo group. The expression of BMP-7 at both the protein and mRNA levels in the rat fracture tissues in all three groups was quantitatively detected by immunohistochemicals SP and RT-PCR. Results revealed that both dicarboxymethyl trisodium alginate and sodium alginate induced the up-regulation of BMP-7 expression. However, the effect of dicarboxymethyl trisodium alginate was superior to that of sodium alginate. Therefore, dicarboxymethyl trisodium alginate can be used to promote the release of BMP-7 from bone cells and contribute to the bone union in traumatic femoral neck fracture in rat models.

Differential Proteomic Identification and Bioinformatics Analysis of Femoral Neck in Elderly Female Patients with Hyperuricaemia

2021 ◽  
Vol 18 ◽  
Author(s):  
Yingyi He ◽  
Guangming Zhang ◽  
Yuyang Huang ◽  
Qi Li ◽  
Cheng Luo
Keyword(s):  
Femoral Neck ◽  
Femoral Neck Fracture ◽  
Differentially Expressed ◽  
Control Group ◽  
Pathophysiological Mechanism ◽  
Differentially Expressed Proteins ◽  
Mineral Density ◽  
Differentially Expressed Protein ◽  
Peptide Mass ◽  
Neck Fracture

Background: Serum uric acid (UA) is positively correlated with bone mineral density (BMD). However, the mechanism by which serum UA affects BMD remains unclear. Objective: The aim was carried out to search for the functional proteins related to serum UA and femoral neck BMD to better understand the pathophysiological mechanism of osteoporosis. Materials and methods: In this study, patients in the UA group (hyperuricaemia combined with femoral neck fracture) and the control group (normal uricaemia combined with femoral neck fracture) were selected according to the inclusion criteria. Total protein was extracted from the femoral neck of each patient. Fluorescence differential gel electrophoresis was used to separate the total proteins, and the differentially expressed protein spots were detected by image analysis. After enzyme digestion, peptide mass fingerprinting and database searches were performed to identify the differentially expressed proteins. DAVID software and Kyoto Encyclopedia of Genes and Genomes (KEGG) data were used for enrichment analysis of the screened differential proteins. Results: After mass spectrometry and database searching, 66 differentially expressed protein spots were identified between the UA group and the control group. Most differentially expressed proteins functioned in cytoskeleton formation, energy metabolism, or signal transduction. They were mainly involved in 50 biological processes, including peroxisome proliferator-activated receptor (PPAR) signalling and fatty acid metabolism. PPARγ and PLIN1 were subject to Western blotting analysis detection; results were consistent with the Label-Free result. Conclusion: Based on an analysis of the biological information, these proteins may be associated with the incidence and progression of the femoral neck bone tissues of hyperuricaemia patients.

scholarly journals Treatment of Pauwels type III femoral neck fracture with medial femoral neck support screw: a biomechanical and clinical study

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zhichao Gao ◽  
Mei Wang ◽  
Baojie Shen ◽  
Xiaodong Chu ◽  
Di Ruan
Keyword(s):  
Femoral Head ◽  
Femoral Neck ◽  
Fracture Healing ◽  
Femoral Neck Fracture ◽  
Biomechanical Study ◽  
Femoral Head Necrosis ◽  
Vertical Shear ◽  
Control Group ◽  
Fixation Failure ◽  
Neck Fracture

AbstractA femoral neck fracture is currently one of the most common types of fracture in clinical practice. The incidence continues to increase due to traffic accidents, trauma, and osteoporosis. This research includes a biomechanical study and a clinical retrospective study. In the biomechanical studies, three groups’ effects (Control Group: 3CCS, DHS group, and study Group: 3CCS + mFNSS group) were compared by vertical compression tests, torsion tests, and fatigue tests. All the data were collected and analyzed. We subsequently performed a retrospective analysis of 131 patients with femoral neck fractures. The operative time, intraoperative blood loss, quality of postoperative fracture reduction, and follow-up observation of fracture healing, screw retreatment rates and fixation failure rates, as well as femoral head necrosis rates and hip function in two groups with 3CCS and 3CCS + mFNSS were compared. By the biomechanical study, we found that 3CCS + Mfnss group were biomechanically superior to 3CCS group and superior to the DHS group in terms of resistance to torsion. However, it was less effective than the DHS group in compressive strength and fatigue resistance. In terms of clinical application, 3CCS + mFNSS group was found to have lower screw retreatment rates and femoral head necrosis rates, and to have better fracture healing rates than group with 3CCS, indicating that medial support screws can effectively resist the vertical shear forces of fracture ends and promote the stability and healing of fracture ends, as well as to reduce the incidence of postoperative complications.

scholarly journals Application of Robot Positioning for Cannulated Screw Internal Fixation in the Treatment of Femoral Neck Fracture: Retrospective Study (Preprint)

2020 ◽  
Author(s):  
Lei Wan ◽  
Xiangyun Zhang ◽  
Dalong Wu ◽  
Zhihao Li ◽  
Dongtao Yuan ◽  
...  
Keyword(s):  
Surgical Treatment ◽  
Femoral Neck ◽  
Internal Fixation ◽  
Fracture Healing ◽  
Femoral Neck Fracture ◽  
Cannulated Screw ◽  
Control Group ◽  
Study Group ◽  
Robot Assisted ◽  
Neck Fracture

BACKGROUND Femoral neck fracture is a common type of hip fracture. Conventional surgical treatment aims at fixing the fracture site with screws and then gradually promoting bone healing. A robot-assisted orthopedic surgery system is computer technology applied to surgical treatment. OBJECTIVE This study aimed to explore the therapeutic effect and prognostic value of percutaneous cannulated screw internal fixation using robot-assisted positioning in patients with femoral neck fractures. METHODS From July 2018 to September 2019, 42 cases of femoral neck fracture admitted to the Second Affiliated Hospital of Luohe Medical College were randomly and averagely divided into control and study groups. The patients in the control group were treated with conventional percutaneous cannulated screw internal fixation, while the patients in the study group were treated with robot-assisted percutaneous cannulated screw fixation during surgical treatment. We compared the treatment conditions and results of the operation between the 2 groups. The Harris score was used to evaluate the treatment efficacy. The state of fracture healing was followed up and compared between the 2 groups. RESULTS The duration of the operation was shorter, there was less fluoroscopy use, and there were fewer drilled holes in the study group than in the control group (all, <i>P</i>&lt;.001). There was no statistical difference in the amount of intraoperative bleeding between the 2 groups (<i>P</i>=.33). The Harris score (<i>P</i>=.045) and number of excellent and good ratings (<i>P</i>=.01) were significantly higher in the study group than in the control group. The difference in the fracture healing rate between the 2 groups was not statistically significant (<i>P</i>=.23). The fracture healing duration of the study group was shorter than that of the control group (<i>P</i>=.001). CONCLUSIONS The use of robotic positioning aids in the treatment of femoral neck fractures with percutaneous cannulated screw fixation can effectively improve the efficiency of surgery, shorten the duration of surgery, and reduce the radiation damage to patients. Meanwhile, it improves postoperative treatment and recovery rates of the patients and shortens the fracture healing time.

scholarly journals Application of Robot Positioning for Cannulated Screw Internal Fixation in the Treatment of Femoral Neck Fracture: Retrospective Study

10.2196/24164 ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. e24164
Author(s):  
Lei Wan ◽  
Xiangyun Zhang ◽  
Dalong Wu ◽  
Zhihao Li ◽  
Dongtao Yuan ◽  
...  
Keyword(s):  
Surgical Treatment ◽  
Femoral Neck ◽  
Internal Fixation ◽  
Fracture Healing ◽  
Femoral Neck Fracture ◽  
Cannulated Screw ◽  
Control Group ◽  
Study Group ◽  
Robot Assisted ◽  
Neck Fracture

Background Femoral neck fracture is a common type of hip fracture. Conventional surgical treatment aims at fixing the fracture site with screws and then gradually promoting bone healing. A robot-assisted orthopedic surgery system is computer technology applied to surgical treatment. Objective This study aimed to explore the therapeutic effect and prognostic value of percutaneous cannulated screw internal fixation using robot-assisted positioning in patients with femoral neck fractures. Methods From July 2018 to September 2019, 42 cases of femoral neck fracture admitted to the Second Affiliated Hospital of Luohe Medical College were randomly and averagely divided into control and study groups. The patients in the control group were treated with conventional percutaneous cannulated screw internal fixation, while the patients in the study group were treated with robot-assisted percutaneous cannulated screw fixation during surgical treatment. We compared the treatment conditions and results of the operation between the 2 groups. The Harris score was used to evaluate the treatment efficacy. The state of fracture healing was followed up and compared between the 2 groups. Results The duration of the operation was shorter, there was less fluoroscopy use, and there were fewer drilled holes in the study group than in the control group (all, P<.001). There was no statistical difference in the amount of intraoperative bleeding between the 2 groups (P=.33). The Harris score (P=.045) and number of excellent and good ratings (P=.01) were significantly higher in the study group than in the control group. The difference in the fracture healing rate between the 2 groups was not statistically significant (P=.23). The fracture healing duration of the study group was shorter than that of the control group (P=.001). Conclusions The use of robotic positioning aids in the treatment of femoral neck fractures with percutaneous cannulated screw fixation can effectively improve the efficiency of surgery, shorten the duration of surgery, and reduce the radiation damage to patients. Meanwhile, it improves postoperative treatment and recovery rates of the patients and shortens the fracture healing time.

scholarly journals Preoperative deep venous thrombosis (DVT) after femoral neck fracture in the elderly, the incidence, timing, location and related risk factors

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Shuai Niu ◽  
Juan Li ◽  
Yan Zhao ◽  
Dianzhu Ding ◽  
Guangwei Jiang ◽  
...  
Keyword(s):  
Risk Factors ◽  
Femoral Neck ◽  
Elderly Patients ◽  
Venous Thrombosis ◽  
Deep Venous Thrombosis ◽  
Femoral Neck Fracture ◽  
Femoral Neck Fractures ◽  
Control Group ◽  
Neck Fracture ◽  
Time From Injury

Abstract Objective To investigate the epidemiologic characteristics of deep venous thrombosis (DVT) in elderly patients with femoral neck fracture. Methods Retrospective analysis was performed on elderly patients with femoral neck fractures admitted to two institutions from January 2016 to October 2019. Duplex ultrasonography (DUS) was used to detect DVT. Patients’ hospitalization medical records were retrieved to collect the data, which were related to demographics, comorbidities, injury and laboratory results on admission. Patients with preoperative DVT were defined as the case group and those without DVT as control group, and compared using the univariate analyses. Multivariate logistic regression analysis was used to identify the independent factors associated with DVT. Results Totally, 980 patients met the predefined criteria and were included. Sixty-seven patients were diagnosed to have preoperative DVT, with incidence of 6.8% for overall, 1.7% for proximal and 5.1% for distal DVT. The mean time from injury to diagnosis of DVT was 6.0 ± 4.7 days (median, 5.0). Most (76.1%) patients with DVT had thrombi solely in the injured extremity, in contrast with 14.9% (10/67) in the uninjured and 9.0% (6/67) in both injured and uninjured extremity. Multivariate analysis showed chronic renal insufficiency (OR, 3.37; 95%CI, 1.57 to 7.28), current smoking status (OR, 2.42; 95%CI, 1.23 to 5.63), time from injury to DUS (OR, 1.26; 95%CI, 1.07 to 1.61) and PLT > 220*109/L (OR, 1.94; 95%CI, 1.31 to 3.77) were independent factors for DVT. Conclusion Preoperative DVT is not very prevalent following elderly femoral neck fractures, but with a certain proportion in the uninjured extremity, necessitating the more attention. These identified risk factors aid in patient counseling, individualized risk assessment and risk stratification, and should be kept in mind.

scholarly journals Efficacy of the Combined Administration of Systemic and Intra-Articular Tranexamic Acid in Total Hip Arthroplasty Secondary to Femoral Neck Fracture: A Retrospective Study

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Joseph Maalouly ◽  
Antonios Tawk ◽  
Rami Ayoubi ◽  
Georges Katoul Al Rahbani ◽  
Aida Metri ◽  
...  
Keyword(s):  
Total Hip Arthroplasty ◽  
Femoral Neck ◽  
Blood Loss ◽  
Tranexamic Acid ◽  
Femoral Neck Fracture ◽  
Hip Arthroplasty ◽  
Control Group ◽  
Transfusion Rate ◽  
Combined Administration ◽  
Neck Fracture

Background. Total hip arthroplasty (THA) is associated with substantial blood loss in the postoperative course. Tranexamic acid (TXA) is a potent antifibrinolytic agent, routinely administered by intravenous (IV) and topical (intra-articular, IA) route, which can possibly interrupt the cascade of events due to hemostatic irregularities close to the source of bleeding. However, scientific evidence of combined administration of TXA in THA secondary to a femoral neck fracture is still meagre. The present study aims to compare the patients who were administered combined IV and topical TXA with a control group in terms of blood loss, transfusion rate, and incidence of deep vein thrombosis (DVT) and thromboembolism (TE). Patients and Methods. 195 patients with femoral neck fracture underwent THA and were placed into two groups: (1) IV and IA TXA group which had 58 patients and (2) no TXA control group which had 137 patients. In the TXA group, 1 g IV TXA was administered 30 minutes before incision, and 1 g IA TXA was administered intraoperatively after fascia closure. No drains were placed, and soft spica was applied to the hip. Results. Combined usage of IV and IA TXA showed better results when compared to the control group in terms of blood transfusion rate (31%) and hemoglobin drop (28%). No cases of DVT or TE were noted among the two study groups. Conclusion. Combined use of IV and IA TXA provided significantly better results compared to no TXA use with respect to all variables related to postoperative blood loss in THA. Moreover, TXA use is safe in terms of incidence of symptomatic DVT and TE.

scholarly journals Biomechanical Evaluation of Reconstruction of Calcar Femorale Structure for Femoral Neck Fractures: A Comparative Finite Element Analysis

2021 ◽  
Author(s):  
Zhi-Hao Gao ◽  
Jian-Xiong Ma ◽  
Ying Wang ◽  
Bin Lu ◽  
Hao-Hao Bai ◽  
...  
Keyword(s):  
Finite Element Analysis ◽  
Finite Element ◽  
Femoral Neck ◽  
Internal Fixation ◽  
Femoral Neck Fracture ◽  
Biomechanical Properties ◽  
Control Group ◽  
Element Analysis ◽  
Neck Fracture ◽  
Reconstruction Nail

Abstract BackgroundThe optimum internal fixation approach for femoral neck fracture therapy is still debated, and there are few studies on calcar femorale repair following femoral neck fracture. The biomechanical properties of various internal fixation systems can be adequately studied using the finite element method. The goal of this work was to use finite element analysis to examine the biomechanical parameters of Pauwels type III femoral neck fractures under various calcar femorale repair nailing procedures.MethodThe finite element approach was used to numerically investigate the calcar femorale reconstruction method for femoral neck fracture repair. A control group with standard inverted triangle nail placement and internal fixation models with calcar femorale reconstruction nails of 150, 155, and 160 degrees, respectively, were constructed. The four groups of models' von Mises stress and displacement distributions are evaluated and compared.ResultsIn the control group and three calcar femorale reconstruction nail angle models, the maximum stress of femur was (MPa): 106.605, 87.317, 147.691, and 102.072 respectively; The peak stress of four kinds of internal fixation were (MPa): 315.121, 228.819, 198.173 and 208.798. In the control group and three calcar femorale reconstruction nail angle models, the maximum displacement of the femur was (mm): 13.19, 13.183, 12.443, and 12.896 respectively; The displacement peaks of the four internal fixations were (mm): 12.646, 12.625, 11.932 and 12.347.ConclusionsAccording to the results of finite element analysis, the biomechanical performance of calcar femorale reconstruction nail with 150 ° is excellent, which is helpful to the reconstruction of calcar femorale structure. This nail placement method can achieve better biomechanical properties. The results of this study can provide a theoretical reference for clinical practice.

scholarly journals Preoperative Deep Venous Thrombosis (DVT) after Femoral Neck Fracture in the Elderly, the Incidence, Timing, Location and Related Risk Factors

2021 ◽  
Author(s):  
Shuai Niu ◽  
Juan Li ◽  
Yan Zhao ◽  
Dianzhu Ding ◽  
Guangwei Jiang ◽  
...  
Keyword(s):  
Risk Factors ◽  
Femoral Neck ◽  
Elderly Patients ◽  
Venous Thrombosis ◽  
Deep Venous Thrombosis ◽  
Femoral Neck Fracture ◽  
Multivariate Logistic Regression Analysis ◽  
Femoral Neck Fractures ◽  
Control Group ◽  
Neck Fracture

Abstract Objective to investigate the epidemiologic characteristics of deep venous thrombosis (DVT) in elderly patients with femoral neck fracture Methods Retrospective analysis was performed on elderly patients with femoral neck fractures admitted to two institutions from January 2016 to October 2019. Duplex ultrasonography (DUS) was used to detect DVT. Patients' hospitalization medical records were retrieved to collect the data, which were related to demographics, comorbidities, injury and laboratory results on admission. Patients with preoperative DVT were defined as the case group and those without DVT as control group, and compared using the univariate analyses. Multivariate logistic regression analysis was used to identify the independent factors associated with DVT. Results Totally, 980 patients met the predefined criteria and were included. Sixty-seven patients were diagnosed to have preoperative DVT, with incidence of 6.8% for overall, 1.7% for proximal and 5.1% for distal DVT. The mean time from injury to diagnosis of DVT was 6.0 ± 4.7 days (median, 5.0). Most (76.1%) patients with DVT had thrombi solely in the injured extremity, in contrast with 14.9% (10/67) in the uninjured and 9.0% (6/67) in both injured and uninjured extremity. Multivariate analysis showed chronic renal insufficiency, current smoking status, delay to DUS and PLT > 220*109/L were independent factors for DVT. Conclusion Preoperative DVT is not very prevalent following elderly femoral neck fractures, but with a certain proportion in the uninjured extremity, necessitating the more attention. These identified risk factors aid in patient counseling, individualized risk assessment and risk stratification, and should be kept in mind.

Evaluation of the correlation between risk factors and mortality in elderly patients with femoral neck fracture with 5-year follow-up

2008 ◽  
Vol 149 (11) ◽  
pp. 493-503 ◽  
Author(s):  
Andor Sebestyén ◽  
Imre Boncz ◽  
Ferenc Tóth ◽  
Márta Péntek ◽  
József Nyárády ◽  
...  
Keyword(s):  
Risk Factors ◽  
Femoral Neck ◽  
Elderly Patients ◽  
Femoral Neck Fracture ◽  
Neck Fracture

A csípőtáji törésekhez idős korban magas halálozás társul. A szakirodalomban kevés a nagy beteganyagot feldolgozó, országos kiterjedésű ellátórendszer adatain alapuló feldolgozás. Célkitűzés: A tanulmány célja a 60 év feletti akut, monotraumás combnyaktöröttek primer ellátását követő halálozások vizsgálata havonta és évente ötéves utánkövetéssel, valamint a különböző rizikófaktoraik halálozásra gyakorolt hatásainak értékelése az egyes időperiódusokban. Módszer: Az adatok az Országos Egészségbiztosítási Pénztár adatbázisából származnak. Az értékelés bázisát a fekvőbeteg-ellátást végző intézményekből combnyaktörés primer műtéti ellátását követően 2000. évben emittált betegek képezik. Bemutatjuk az átlagos évenkénti, havonkénti és heti halálozási arányokat, valamint rizikótényezők szerinti alakulásukat havonként és évenként. A rizikótényezők és a halálozás kapcsolatának értékelése logisztikus és Cox-regressziós analízissel történik. Eredmények: A tanulmányban 3783 fő került elemzésre. Átlagéletkoruk 77,97 (SD 8,52) év. A halálozás az első héten 1,71%, 30 napon belül 8,99%, az első évben 30,74%, öt év alatt 61,88% volt. A halálozás havi szinten az első 5 hónapig mutat csökkenést, éves szinten az első év után stagnál. A rizikófaktorok közül a férfinem és a magasabb életkor öt évig, a kísérőbetegségek hatásai a negyedik évig, a laterális combnyaktöréstípus és a 12 órán túli ellátás két évig, a korai lokális szövődmények egy évig, a hétvégi ellátások az első hónapban eredményeznek magasabb halálozási kockázatot. Az országos és egyetemi ellátásokat követően az első évben alacsonyabb a halálozási kockázat. Következtetések: A csípőtáji törések managementjében a halálozások csökkentése érdekében hangsúlyozzuk a 12 órán belüli ellátás, a törési típusnak megfelelő módszerválasztás, a hét minden napján történő azonos ellátási feltételek biztosítása, az ellátások centrumokba történő szervezése, a beteg általános állapotának és kísérőbetegségeinek megfelelő akut ellátás és az utókezelések fontosságát.

Sign in / Sign up

Export Citation Format

Share Document