Antioxidant and ROS inhibitory activities of heterocyclic 2-Aryl-4(3H)-quinazolinone derivatives

Background: Antioxidants are small molecules that prevent or delay the process of oxidations caused by highly reactive free radicals. These molecules are known for their ability to protect various cellular architecture and other biomolecules from oxidative stress and free radicals. Thus, antioxidants play a key role in the prevention of oxidative damages caused by highly reactive free radicals. Method: In the present study, a series of previously synthesized heterocyclic 2-aryl-4(3H)-quinazolinone derivatives 1-25 was screened for antioxidant activity by employing in vitro DPPH and superoxide anion radical scavenging activities. ROS inhibitory activities were also evaluated by serum-opsonized zymosan activated whole blood phagocytes and isolated neutrophils. Cytotoxicity studies were carried out by employing an MTT assay against the 3T3 cell line. Results: Most of the 2-aryl-4(3H)-quinazolinone derivatives showed potent antioxidant activities in superoxide anion radical scavenging assay with IC50 value ranging between 0.57 µM – 48.93 µM, as compared to positive control quercetin dihydrate (IC50 = 94.1± 1.1 µM ). Compounds 5, 6, and 14 showed excellent activity in DPPH assay. Compounds 5-8, 12-15, 17, and 20 showed promising activities in the ROS inhibition assay. All compounds were found to be non-cytotoxic against the 3T3 cell line. Structure antioxidant activity has been established. Conclusion: It can be concluded that most of the heterocyclic 2-aryl-4(3H)-quinazolinone derivatives 1-25 are identified as promising antioxidant agents that are capable of fighting against free radicals and oxidative stress. Thus, they can serve as a lead towards treating oxidative stress and related pathologies.

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Effect of extraction conditions of paprika oleoresins on their free radical scavenging and anticancer activity

Open Chemistry ◽

10.2478/s11532-013-0378-1 ◽

2014 ◽

Vol 12 (3) ◽

pp. 377-385 ◽

Cited By ~ 4

Author(s):

Vesna Šaponjac ◽

Dragana Četojević-Simin ◽

Gordana Ćetković ◽

Jasna Čanadanović-Brunet ◽

Sonja Djilas ◽

...

Keyword(s):

Carbon Dioxide ◽

Supercritical Carbon Dioxide ◽

Cell Line ◽

Antiproliferative Activity ◽

Superoxide Anion ◽

Anion Radical ◽

Radical Scavenging ◽

Superoxide Anion Radical ◽

Food Ingredients ◽

Supercritical Carbon

AbstractGround spice paprika was extracted with hexane, by conventional Soxhlet procedure (SX oleoresin), and with supercritical carbon dioxide at three different pressures — 20, 30 and 40 MPa (SF20, SF30 and SF40 oleoresins). The effect of extraction method and conditions on the colour intesity of paprika oleoresins, content of α-tocopherol, as well as antioxidant and antiproliferative activity was examined. Hexane showed highest selectivity for paprika pigments (886.02 ASTA), while α-tocopherol showed highest solubility (3846.9 mg kg−1) in supercritical carbon dioxide at 20 MPa. All paprika oleoresins exhibited good superoxide anion radical scavenging activity SF30 being the best superoxide anion radical scavenger. Cell growth activity was evaluated in vitro in human cell lines:cervix epitheloid carcinoma (HeLa), breast adenocarcinoma (MCF7) and colon adenocarcinoma (HT-29). The highest antiproliferative activity was exhibited by SX in MCF7 cell line (IC50=14.28 mg mL−1). Extract SF40 produced significant and selective antiproliferative action towards HeLa cell line. These results suggest that paprika oleoresins, due to high antiradical and tumor cell-inhibiting activity, can be regarded as functional food ingredients.

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Researches on the Structure and Antioxidant Activity of Apigenin-La (III) Complex

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.781-784.1138 ◽

2013 ◽

Vol 781-784 ◽

pp. 1138-1143

Author(s):

Feng Qiu Wen ◽

Lang Sheng Pan ◽

Lei Lei

Keyword(s):

Thermal Analysis ◽

Antioxidant Activity ◽

Free Radicals ◽

Superoxide Anion ◽

Anion Radical ◽

Superoxide Anion Radical ◽

Scavenging Activity ◽

Scavenging Effect ◽

Pyrogallic Acid ◽

Better Than

La (III) complex with apigenin was synthesized and characterized by UV, IR, 1HNMR and thermal analysis. The scavenging activity of the complex on superoxide anion radical (O2-·) and hdroxyl radical (·OH) was also investigated by improved pyrogallic acid way and ortho-oxybenzoic acid way. The results show that the complex had remarkable scavenging effects on O2-· and ·OH, and its scavenging effect on free radicals was better than apigenin.

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Anti-Oxidant Activity of Anthocyanins of Black Soybean In Vitro

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.634-638.1423 ◽

2013 ◽

Vol 634-638 ◽

pp. 1423-1428 ◽

Cited By ~ 2

Author(s):

Yan Song ◽

Guo Ping Yu ◽

Chao Ran Dou ◽

Hao Wu ◽

Zhu Gong

Keyword(s):

Antioxidant Activity ◽

Superoxide Anion ◽

Anion Radical ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Superoxide Anion Radical ◽

Scavenging Activity ◽

Antioxidant Power ◽

Black Soybean

The aim of the work was to research antioxidant activity of the anthoyanins extracts of black soybean. The whole soybean through defatted, were to determine the phenolic and anthocyanin contents in black soybean extracts and to examine antioxidant activity of extract against DPPH and ABTS assays, and Ferric Reducing Antioxidant Power (FRAP), and superoxide anion radical scavenging activity. The defatted sample was extracted using ethanol by ultrasonic. The optimum DPPH and ABTS radical scavenging capacity (%) were 68.34% and 70.12%, VC was 59.76% and 95.23%, respectively, and contains high levels of reducing power and superoxide anion radical scavenging activity. The present results indicate that the anthoyanins extract from black soybean has greater anti-oxidative and is useful in functional food and other applications.

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A Study of Antioxidant Activity about Seleno-Polysaccharide of Cordyceps militaris from Different Areas of China

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.807-809.1984 ◽

2013 ◽

Vol 807-809 ◽

pp. 1984-1987 ◽

Cited By ~ 1

Author(s):

Qin Li ◽

Chi Zhang

Keyword(s):

Antioxidant Activity ◽

Hydroxyl Radical ◽

Superoxide Anion ◽

Anion Radical ◽

Reducing Power ◽

Cordyceps Militaris ◽

Superoxide Anion Radical ◽

Scavenging Capacity ◽

Research Objects ◽

Total Selenium

In this article,Cordyceps militaris,that from Hubei and Yunnan,were used as the research objects, to extract seleno-polysaccharide ,then the antioxidant activity of seleno-polysaccharide were determined by reducing power,the scavenging capacity on superoxide anion radical and hydroxyl radical.The results indicated that,the content of selenium in Hubei and Yunnan Cordyceps militaris samples were 210.0μg/g and 57.5μg/g,respectively;the share of Se-polysaccharide in total selenium were 11.086% and 26.713%,respectively,lower degree to turn the selenium organic in Hubei Cordyceps militaris.The reducing power of seleno-polysaccharide from Hubei and Yunnan Cordyceps militaris were 0.0253,0.0297,respectively;two kinds of Cordyceps militaris have certain ability to remove superoxide anion radical and different between them,the seleno-polysaccharide of Yunnan Cordyceps militaris has a better effect to remove superoxide anion radical under different sample amount.About hydroxyl radical,there were no ability to remove it.

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Study on Scavenging Free Radical Activity with Polysaccharides Materials in Chuanxiong Based on Composite Properties of Biomaterials

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.583.244 ◽

2012 ◽

Vol 583 ◽

pp. 244-247

Author(s):

Yong Guang Bi ◽

Chun Chun Liu

Keyword(s):

Free Radical ◽

Superoxide Anion ◽

Anion Radical ◽

Radical Scavenging ◽

Natural Antioxidants ◽

Superoxide Anion Radical ◽

Dose Effect ◽

Spectrophotometric Detection ◽

Free Radical Activity ◽

Composite Properties

By using spectrophotometric detection of the Chuanxiong polysaccharides on the free radical scavenging. The results show that the Chuanxiong polysaccharides on DPPH radical, hydroxyl radical (HO•) and superoxide anion radical (•) clearance. Scavenging ability with the the Chuanxiong polysaccharide concentration increased, and showed the dose-effect relationship. Concentration of 2.0mg/mL when DPPH radicals clear the rate of 35.61%, while the rate of hydroxyl radicals and superoxide anion radical scavenging, clear the rate of 57.78% and 57.14%. Chuanxiong polysaccharide is an ideal natural antioxidants and good prospects for the development of biomedical composites.

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