Exploring the Protective Effect of ShenQi Compound on Skeletal Muscle in Diabetic Macrovasculopathy Mice

Objective: ShenQi compound (SQC) is a traditional herbal formula, which has been used to treat Type 2 diabetes mellitus (T2DM) and complications for years. The aim of this study was to explore the preventive and protective effects of SQC recipe on the skeletal muscle of diabetic macrovasculopathy mice, which provides a theoretical basis for the clinical use of this formula. Methods: We evaluated the effect of SQC in a diabetic vasculopathy mouse model by detecting a series of blood indicators (blood glucose, lipids and insulin) and performing histological observations. Meanwhile, we explored the molecular mechanism of SQC treatment on skeletal muscle by genome expression profiles. Results: The results indicated that SQC could effectively improve blood glucose, serum lipids (total cholesterol (TC), Triglyceride (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C)) and insulin (INS) levels in diabetic vasculopathy mice, as well as alleviating skeletal muscle tissue damage for diabetic macrovasculopathy. Meanwhile, compared with rosiglitazone, SQC showed a better effect on blood glucose fluctuation. Moreover, the gene microarray analysis indicated that SQC might improve T2DM by affecting biological functions related to cell death and cell adhesion. Moreover, 7 genes (Celsr2, Rilpl1, Dlx6as, 2010004M13Rik, Anapc13, Gm6097, Ddx39b) might be potential therapeutic targets of SQC. Conclusion: All these results indicate that SQC is an effective preventive and protective drug for skeletal muscle in diabetic macrovasculopathy, and could alleviate skeletal muscle tissue damage through affecting biological functions related to cell death and cell adhesion.

Download Full-text

Serum and urinary markers of skeletal muscle tissue damage after weight lifting exercise

European Journal of Applied Physiology and Occupational Physiology ◽

10.1007/bf00637392 ◽

1989 ◽

Vol 58 (7) ◽

pp. 786-790 ◽

Cited By ~ 35

Author(s):

G. L. Paul ◽

J. P. DeLany ◽

J. T. Snook ◽

J. G. Seifert ◽

T. E. Kirby

Keyword(s):

Skeletal Muscle ◽

Muscle Tissue ◽

Tissue Damage ◽

Weight Lifting ◽

Skeletal Muscle Tissue ◽

Urinary Markers

Download Full-text

The Effects of Estrogen on Indices of Skeletal Muscle Tissue Damage after Eccentric Exercise in Postmenopausal Women

Human Physiology ◽

10.1023/b:hump.0000036343.54390.0f ◽

2004 ◽

Vol 30 (4) ◽

pp. 462-466 ◽

Cited By ~ 1

Author(s):

J. D. Dobridge ◽

A. C. Hackney

Keyword(s):

Skeletal Muscle ◽

Postmenopausal Women ◽

Muscle Tissue ◽

Eccentric Exercise ◽

Tissue Damage ◽

Skeletal Muscle Tissue

Download Full-text

Skeletal Muscle Tissue Damage Leads to a Conserved Stress Response and Stem Cell-Specific Adaptive Transitions

SSRN Electronic Journal ◽

10.2139/ssrn.3537798 ◽

2020 ◽

Author(s):

Léo Machado ◽

Matthieu Dos Santos ◽

Jordi Camps ◽

Jens Van Herck ◽

Hugo Varet ◽

...

Keyword(s):

Skeletal Muscle ◽

Stem Cell ◽

Stress Response ◽

Muscle Tissue ◽

Tissue Damage ◽

Skeletal Muscle Tissue

Download Full-text

Recycled algae-based carbon materials as electroconductive 3D printed skeletal muscle tissue engineering scaffolds

Journal of Materials Science Materials in Medicine ◽

10.1007/s10856-021-06534-6 ◽

2021 ◽

Vol 32 (7) ◽

Author(s):

Selva Bilge ◽

Emre Ergene ◽

Ebru Talak ◽

Seyda Gokyer ◽

Yusuf Osman Donar ◽

...

Keyword(s):

Skeletal Muscle ◽

Tissue Engineering ◽

Electrical Stimulation ◽

Muscle Tissue ◽

Carbonaceous Material ◽

Hydrothermal Carbonization ◽

Skeletal Muscle Tissue ◽

Myotube Formation ◽

3D Printed

AbstractSkeletal muscle is an electrically and mechanically active tissue that contains highly oriented, densely packed myofibrils. The tissue has self-regeneration capacity upon injury, which is limited in the cases of volumetric muscle loss. Several regenerative therapies have been developed in order to enhance this capacity, as well as to structurally and mechanically support the defect site during regeneration. Among them, biomimetic approaches that recapitulate the native microenvironment of the tissue in terms of parallel-aligned structure and biophysical signals were shown to be effective. In this study, we have developed 3D printed aligned and electrically active scaffolds in which the electrical conductivity was provided by carbonaceous material (CM) derived from algae-based biomass. The synthesis of this conductive and functional CM consisted of eco-friendly synthesis procedure such as pre-carbonization and multi-walled carbon nanotube (MWCNT) catalysis. CM obtained from biomass via hydrothermal carbonization (CM-03) and its ash form (CM-03K) were doped within poly(ɛ-caprolactone) (PCL) matrix and 3D printed to form scaffolds with aligned fibers for structural biomimicry. Scaffolds were seeded with C2C12 mouse myoblasts and subjected to electrical stimulation during the in vitro culture. Enhanced myotube formation was observed in electroactive groups compared to their non-conductive counterparts and it was observed that myotube formation and myotube maturity were significantly increased for CM-03 group after electrical stimulation. The results have therefore showed that the CM obtained from macroalgae biomass is a promising novel source for the production of the electrically conductive scaffolds for skeletal muscle tissue engineering.

Download Full-text