Analysis of Brassinosteroids in Soybean Seeds and Leaves by Liquid Chromatography-Tandem Mass Spectrometry
Keyword(s):
Objective:A simple and fast high performance liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS) method has been developed for the analysis of brassinosteroids (BRs) in plants without derivatization.Materials:The BRs (including castasterone, 24-epicastasterone, brassinolide and 24-epibrassinolide) have been extracted with ice cold 80% aqueous methanol solution.Method:Five different purification strategies have been tested for the purification and enrichment of BRs.Conclusion:This analytical method was sensitive, reliable, rapid and applicable to trace analysis in complex plant samples.
2006 ◽
2013 ◽
Vol 41
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pp. 1147
2013 ◽
Vol 40
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pp. 1764-1771
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2013 ◽
Vol 30
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pp. 154-159
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2013 ◽
Vol 31
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pp. 1224
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2013 ◽
Vol 30
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pp. 160-164
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