Directed Differentiation of Pluripotent Cells Towards Therapeutic Stem Cells

Xeno-free culture systems have expanded the clinical and industrial application of human pluripotent stem cells (PSCs). However, yet some problems, such as the reproducibility among the experiments, remain. Here we describe an improved method for the subculture of human PSCs. The revised method significantly enhanced the viability of human PSCs by lowering DNA damage and apoptosis, resulting in more efficient and reproducible downstream applications such as gene editing, gene delivery, and directed differentiation. Furthermore, the method did not alter PSC characteristics after long-term culture and attenuated the growth advantage of abnormal subpopulations. This robust passaging method minimizes experimental error and reduces the rate of PSCs failing quality control of human PSC research and application.

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Directed differentiation of mouse-induced pluripotent stem cells generates dopaminergic nerve

Cell Biology International ◽

10.1042/cbi034s036c ◽

2010 ◽

Vol 34 (8) ◽

pp. S36-S36

Author(s):

Ping Duan ◽

Xuelin Ren ◽

Wenhai Yan ◽

Xuefei Han ◽

Xu Yan ◽

...

Keyword(s):

Stem Cells ◽

Induced Pluripotent Stem Cells ◽

Pluripotent Stem Cells ◽

Directed Differentiation ◽

Induced Pluripotent

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Faculty Opinions recommendation of Directed differentiation and functional maturation of cortical interneurons from human embryonic stem cells.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.718007791.793478218 ◽

2013 ◽

Author(s):

Bill Lowry

Keyword(s):

Stem Cells ◽

Embryonic Stem Cells ◽

Human Embryonic Stem Cells ◽

Embryonic Stem ◽

Directed Differentiation ◽

Functional Maturation ◽

Cortical Interneurons

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Chemically defined and xeno-free culture condition for human extended pluripotent stem cells

Nature Communications ◽

10.1038/s41467-021-23320-8 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Bei Liu ◽

Shi Chen ◽

Yaxing Xu ◽

Yulin Lyu ◽

Jinlin Wang ◽

...

Keyword(s):

Stem Cells ◽

Pluripotent Stem Cells ◽

Culture Condition ◽

Human Fibroblast ◽

Culture System ◽

Disease Modeling ◽

Directed Differentiation

AbstractExtended pluripotent stem (EPS) cells have shown great applicative potentials in generating synthetic embryos, directed differentiation and disease modeling. However, the lack of a xeno-free culture condition has significantly limited their applications. Here, we report a chemically defined and xeno-free culture system for culturing and deriving human EPS cells in vitro. Xeno-free human EPS cells can be long-term and genetically stably maintained in vitro, as well as preserve their embryonic and extraembryonic developmental potentials. Furthermore, the xeno-free culturing system also permits efficient derivation of human EPS cells from human fibroblast through reprogramming. Our study could have broad utility in future applications of human EPS cells in biomedicine.

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