scholarly journals Spectrofluorimetric Determination of Some N. Containing Medicines Using Rhodamine 6G as a Chromogenic Reagent

2021 ◽  
Vol 22 (2) ◽  
pp. 367-375
Author(s):  
Theia'a Najim Al-Sabha ◽  
Mohamed Yahya Dhamra
Keyword(s):  
Fluorescence Intensity ◽  
Rhodamine 6G ◽  
Pharmaceutical Preparations ◽  
Detection Limits ◽  
Ion Pair ◽  
Amino Groups ◽  
Spectrofluorimetric Method ◽  
Spectrofluorimetric Determination ◽  
Tertiary Amino

A sensitive spectrofluorimetric method has been developed for the analysis of some medicines containing primary, secondary, and tertiary amino groups, namely Diclofenac (DIC), Domperidone (DOM), Famotidine (FAM), and Propranolol (PRO), in their pure and medicinal forms. The method is based on the quenching of the fluorescence intensity of rhodamine 6G (R6G) through the formation of ion-pair complexes between the above medicines and the R-6G reagent, which is measured at 552 nm after excitation at 402 nm. The calibration graphs were rectilinear in the concentration ranges of 0.10- 9.00, 0.05-15.00, 0.10-14.0 and 0.05-5.00 µg mL-1 for above medicines respectively. The recovery (%) values were ranged between 99.45%- 100.97%. The detection limits ranged in the concentration of 0.243-0.754 µg/mL, and the limits of quantitation were 0.806- 2.420 µgmL-1 for all drugs. The method was successfully applied for the determination of these drugs in their pharmaceutical preparations.

Spectrofluorimetric Determination of Bilirubin Using Enoxacine-Terbium Probe

2014 ◽  
Vol 556-562 ◽  
pp. 421-424 ◽  
Author(s):  
Wei Wei Bian
Keyword(s):  
Detection Limit ◽  
Fluorescence Intensity ◽  
Trace Amount ◽  
Optimum Conditions ◽  
Serum Samples ◽  
Buffer Solution ◽  
Spectrofluorimetric Method ◽  
Spectrofluorimetric Determination ◽  
Terbium Ion

A new spectrofluorimetric method was developed for determination of trace amount of bilirubin. Using enoxacine–terbium ion as a fluorescent probe, in the buffer solution of pH=5.8, BR can remarkably reduce the fluorescence intensity of the ENX-Tb3+ complex at λ=545nm and the reduced fluorescence intensity of Tb3+ ion is in proportion to the concentration of BR. Optimum conditions for the determination of BR were also investigated. The linear range and detection limit for the determination of BR are 1.0×10-7～4.5×10-6mol/L and 8.1×10-8mol/L. This method is simple, practical and can be successfully applied to assess BR in serum samples.

Application of the Stern–Volmer equation for studying the spectrofluorimetric quenching reaction of eosin with clindamycin hydrochloride in its pure form and pharmaceutical preparations

2015 ◽  
Vol 7 (24) ◽  
pp. 10445-10451 ◽  
Author(s):  
M. E. K. Wahba ◽  
N. El-Enany ◽  
F. Belal
Keyword(s):  
Pharmaceutical Preparations ◽  
Pure Form ◽  
Eosin Y ◽  
Amino Group ◽  
Spectrofluorimetric Method ◽  
Tertiary Amino

A sensitive, selective, economic, and validated spectrofluorimetric method was developed for the determination of clindamycin hydrochloride in pharmaceutical preparations, depending on the reaction of its tertiary amino group with eosin Y.

scholarly journals Spectrofluorometric determination of nicardipine, nifedipine and isradipine in pharmaceutical preparations and biological fluids

2008 ◽  
Vol 6 (2) ◽  
pp. 222-228 ◽  
Author(s):  
Sheikha Al-Ghannam ◽  
Abeer Al-Olyan
Keyword(s):  
Fluorescence Intensity ◽  
Reaction Pathway ◽  
Biological Fluids ◽  
Sodium Dodecyl ◽  
Pharmaceutical Preparations ◽  
Reduction Reaction ◽  
Factors Affecting ◽  
Plasma And Urine Samples

AbstractA simple and highly sensitive spectrofluorometric method was developed for the determination of some 1,4-dihydropyridine compounds namely, nicardipine, nifedipine and isradipine in pharmaceutical preparations and biological fluids. The method is based on the reduction of nicardipine, nifedipine and isradipine with Zn/HCl and measuring the fluorescence intensity obtained (λem/λex) at 460/364, 450/393 and 446/360 nm, respectively. The factors affecting the development of the fluorophore and its stability were studied and optimized. The effect of some surfactants such as β-cyclodextrin (βCD), carboxymethylcelullose (CMC), sodium dodecyl sulphate (SDS) and triton X-100, on the fluorescence intensity was studied. The fluorescence intensity-concentration plots of nicardipine, nifedipine and isradipine were rectilinear over the ranges 0.4–6.0, 0.2–4.0 and 0.1–9.0 μg ml−1 with detection limits of 0.0028, 0.017 and 0.016 μg ml−1, respectively. The proposed method was successfully applied to commercial tablets containing the compounds; the percentage recovery agreed well with those obtained using the official methods. The method was further extended to the in vitro determination of the compounds in spiked human plasma and urine samples. A proposal of the reduction reaction pathway was postulated.

scholarly journals Development and validation of a new spectrofluorimetric method for the determination of some beta-blockers through fluorescence quenching of eosin Y. Application to content uniformity test

2016 ◽  
Vol 14 (1) ◽  
pp. 258-266 ◽  
Author(s):  
Sayed M Derayea ◽  
Mahmoud A Omar ◽  
Mohamed Aboel-Kasem Abdel-Lateef ◽  
Ahmed I. Hassan
Keyword(s):  
Fluorescence Quenching ◽  
Beta Blockers ◽  
Dosage Forms ◽  
Ion Pair ◽  
Content Uniformity ◽  
Eosin Y ◽  
Pharmaceutical Dosage Forms ◽  
Quenching Effect ◽  
Spectrofluorimetric Method

AbstractA simple, rapid, sensitive and economic spectrofluorimetric method has been developed and validated for determination of some β-adrenergic blocking agents namely; betaxolol hydrochloride (BTX), carvedilol (CAR), labetalol hydrochloride (LBT), nebivolol hydrochloride (NEB) and propranolol hydrochloride (PRO). The method is based on the quenching effect of the cited drugs on the fluorescence intensity of eosin Y at pH 3.4 (acetate buffer). The fluorescence quenching is due to the formation of an ion-pair complex and was measured without extraction at 545 nm (λex. 301.5 nm). The factors affecting the formation of the ion-pair complex were carefully studied and optimized. Under the optimal conditions, the linear ranges for the relationship between the fluorescence quenching value and the concentration of the investigated drugs were 100-2500, 150-2500 and 50-2250 ng mL-1 for (BTX, CAR), (LBT, NEB) and (PRO) respectively. The method was validated according to ICH guidelines and was applied for determination of the cited drugs in pharmaceutical dosage forms with excellent recoveries. In addition, content uniformity testing of some commercial dosage forms was checked by the proposed method.

Rapid Colorimetric Assay of Trimethoprim and Sulfamethoxazole in Pharmaceuticals

1983 ◽  
Vol 66 (6) ◽  
pp. 1447-1449 ◽  
Author(s):  
Asis K Sanyal ◽  
Dinabandhu Laha
Keyword(s):  
Solvent Extraction ◽  
Colorimetric Assay ◽  
Pharmaceutical Preparations ◽  
Ion Pair ◽  
Pair Formation ◽  
Bromophenol Blue ◽  
Colorimetric Determination ◽  
Ion Pair Formation ◽  
Subsequent Measurement

Abstract A method is described for the direct colorimetric determination of trimethoprim and sulfamethoxazole in pharmaceutical preparations, without prior separation. Estimation of trimethoprim is based on its ion-pair formation with bromophenol blue and subsequent measurement of absorbance of the ionpair at 418 nm. Estimation of sulfamethoxazole is possible without removal of trimethoprim by solvent extraction.

scholarly journals Spectrofluorimetric Determination of Warfarin Sodium by Using N1-Methylnicotinamide Chloride as a Fluorigenic Agent

2005 ◽  
Vol 88 (2) ◽  
pp. 455-461 ◽  
Author(s):  
Mohamed A El Dawy ◽  
Mokhtar M Mabrouk ◽  
Riad A El Barbary
Keyword(s):  
Aqueous Solutions ◽  
Human Plasma ◽  
Plasma Samples ◽  
Spiked Human Plasma ◽  
Human Plasma Samples ◽  
Warfarin Sodium ◽  
Spectrofluorimetric Method ◽  
Spectrofluorimetric Determination ◽  
Methylene Groups

Abstract A spectrofluorimetric method is described for the determination of drugs containing active methylene groups adjacent to carbonyl groups. The method was applied successfully to the determination of warfarin sodium in laboratory-prepared mixtures, in commercial tablets, and in spiked human plasma samples. Finally, the method was applied to the determination of the steady-state concentration of warfarin sodium in the blood of a hospitalized patient. The method involves the reaction of warfarin sodium with 0.2 ml (0.4 × 10−3M) N1-methylnicotinamide chloride reagent in the presence of 3 mL 1.0N NaOH and cooling in ice for 8 min, followed by adjustment of the pH to 2.0, using formic acid and heating for 4 min, whereby a highly fluorescent reaction product is produced. The optimal wavelengths of excitation and emission were determined by using a synchronous wavelength search and found to be 284 and 354 nm, respectively. The standard curves were linear over a concentration range of 50–1500 ng/mL in both aqueous solutions and spiked human plasma samples. The mean recoveries (± standard deviation) were 101.157 (±1.33) and 95.73 (±1.88%) for aqueous solutions and spiked human plasma samples, respectively. The method showed good specificity and precision. The proposed method is simple and economical because of its minimal instrumentation and chemicals requirements. Nevertheless, it is highly sensitive, specific, and reproducible. Accordingly, it is suitable for quality-control applications, drug monitoring, and bioavailability and bioequivalency studies.

Sign in / Sign up

Export Citation Format

Share Document