Molecular markers and their potentials

2016 ◽  
Vol 5 (01) ◽  
pp. 4706 ◽  
Author(s):  
Arifa Khatoon ◽  
Sumeet Verma ◽  
Gayatri Wadiye ◽  
Anuprita Zore

The use of molecular markers, revealing polymorphism at the DNA level, has been playing an increasing part in plant molecular biotechnology and their genetic studies. There are three different types of markers viz. morphological, biochemical and DNA based molecular markers. These DNA based markers are differentiating in two types 1. Non PCR based (RFLP) and 2. PCR based markers (RAPD, AFLP, SSR, SNP etc.). Amongst others, the microsatellite DNA marker is one of the most widely used marker due to its easy use by simple PCR, followed by a denaturing gel electrophoresis. SNP (Single Nucleotide Polymorphism) is nowadays is the one which is used mainly. In this review, we are going to discuss about the biochemical and molecular markers which are recently developed, the important characteristics of molecular markers their advantages, disadvantages and the applications of these markers in comparison with other markers types.

2013 ◽  
Vol 13 (1) ◽  
pp. 197 ◽  
Author(s):  
Mir A Iquebal ◽  
Sarika   ◽  
Vasu Arora ◽  
Nidhi Verma ◽  
Anil Rai ◽  
...  

2011 ◽  
Vol 108 (3) ◽  
pp. 347-354 ◽  
Author(s):  
Sivaramakurup SREEKUMAR ◽  
Southekal K. ASHWATH ◽  
Monika SLATHIA ◽  
Sundaramurthy N. KUMAR ◽  
Syed M.H. QADRI

BMC Genetics ◽  
2013 ◽  
Vol 14 (1) ◽  
pp. 118 ◽  
Author(s):  
Mir Iquebal ◽  
Null Sarika ◽  
Sandeep Dhanda ◽  
Vasu Arora ◽  
Sat Dixit ◽  
...  

Database ◽  
2015 ◽  
Vol 2015 ◽  
pp. bav111 ◽  
Author(s):  
Mir Asif Iquebal ◽  
Sarika Jaiswal ◽  
U.B. Angadi ◽  
Gaurav Sablok ◽  
Vasu Arora ◽  
...  

2003 ◽  
Vol 82 (2) ◽  
pp. 101-106 ◽  
Author(s):  
SOOJIN YI ◽  
BRIAN CHARLESWORTH

We have investigated the pattern of DNA sequence variation at the exuperantia2 locus in Drosophila pseudoobscura. This adds to the increasing dataset of genetic variation in D. pseudoobscura, a useful model species for evolutionary genetic studies. The level of silent site nucleotide diversity and the divergence from an outgroup Drosophila miranda are comparable with those for other X-linked loci. One peculiar pattern at the exu2 locus of D. pseudoobscura is a complete linkage disequilibrium between two SNPs, one of which is a replacement site. As a result, there are two distinct haplotype groups in our dataset. Based upon the comparisons with the outgroup sequences from D. miranda and Drosophila persimilis, we show that the newly derived haplotype group has lower diversity than the ancestral haplotype group. The pattern of protein evolution at exu2 shows some deviation from the neutral model. Together, these and other characteristics of the exu2 locus suggest the action of selection on the pattern of SNP variation, consistent with a partial selective sweep associated with the newly derived haplotype.


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