A study on the antifungal susceptibility pattern of dermatophytes isolated in a tertiary care hospital

Recent years, due to increased usage of antifungal treatment worldwide, there is an increased chance of rising resistance among antifungal drugs too. Dermatophytic infections causes’ superficial mycosis and it affects skin, hair and nail. These infections are more common and antifungal drugs are used everywhere to treat those common infections. To conduct a study by determining the antifungal susceptibility pattern in dermatophytic isolates from patients attending dermatology OPD in a tertiary care hospital. A total of 217 samples like hair, nail and skin scrapings were obtained and isolation of dermatophytes was done. Antifungal susceptibility testing for dermatophytes was performed by micro broth dilution method. Antifungal drugs tested were Griseofulvin, Fluconazole, Itraconazole and Ketoconazole. Minimum inhibitory concentration for each drug for fungal isolates was tested and results studied. Fluconazole showed a higher MIC values in the range of 1-8µg/ml. Itraconazole showed the lowest MIC values by micro broth dilution method. Since there is limitation of standard guidelines and protocol, meticulous research must be conducted on effect of antifungals and derive at universally implementable guidelines.

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To study the profile of Candida isolates and antifungal susceptibility pattern of neonatal sepsis in a tertiary care hospital of North India

The Journal of Maternal-Fetal & Neonatal Medicine ◽

10.1080/14767058.2019.1670799 ◽

2019 ◽

pp. 1-5

Author(s):

Mamta Lamba ◽

Deepak Sharma ◽

Rajni Sharma ◽

Aruna Vyas ◽

Ved Mamoria

Keyword(s):

Neonatal Sepsis ◽

Tertiary Care Hospital ◽

Antifungal Susceptibility ◽

Tertiary Care ◽

North India ◽

Care Hospital ◽

Susceptibility Pattern

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Oral Candidiasis among Cancer Patients Attending a Tertiary Care Hospital in Chennai, South India: An Evaluation of Clinicomycological Association and Antifungal Susceptibility Pattern

Canadian Journal of Infectious Diseases and Medical Microbiology ◽

10.1155/2016/8758461 ◽

2016 ◽

Vol 2016 ◽

pp. 1-6 ◽

Cited By ~ 8

Author(s):

Abirami Lakshmy Jayachandran ◽

Radhika Katragadda ◽

Ravinder Thyagarajan ◽

Leela Vajravelu ◽

Suganthi Manikesi ◽

...

Keyword(s):

Cancer Patients ◽

Amphotericin B ◽

Oral Candidiasis ◽

Antifungal Susceptibility ◽

Tertiary Care ◽

Antifungal Drug ◽

Dilution Method ◽

Care Hospital ◽

Susceptibility Pattern

Oropharyngeal candidiasis is one of the common manifestations seen in cancer patients on cytotoxic therapy and invasion into deeper tissues can occur if not treated promptly. Emergence of antifungal drug resistance is of serious concern owing to the associated morbidity and mortality. The present study aims at evaluation of clinicomycological association and antifungal drug susceptibility among the 180 recruited patients with cancer on chemotherapy and/or radiotherapy with signs or symptoms suggestive of oral candidiasis. Speciation and antifungal susceptibility was done by Microbroth dilution method for fluconazole, Itraconazole, and Amphotericin B as per standard microbiological techniques. Chi-square test was used for statistical analysis (p<0.05was considered statistically significant).Candida albicanswas the predominant species isolated (94) (58%) followed byCandida tropicalis(34) (20.9%). Fluconazole and Itraconazole showed an overall resistance rate of 14% and 14.8%, respectively. All the isolates were susceptible to Amphotericin B. There was a significant association between the presence of dry mouth and isolation ofCandida(p<0.001). Such clinicomicrobiological associations can help in associating certain symptoms with the isolation ofCandida. Species level identification with in vitro antifungal susceptibility pattern is essential to choose the appropriate drug and to predict the outcome of therapy.

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Surveillance and characterization of Candida bloodstream infections in a Serbian tertiary care hospital

The Journal of Infection in Developing Countries ◽

10.3855/jidc.7970 ◽

2016 ◽

Vol 10 (06) ◽

pp. 643-656 ◽

Cited By ~ 10

Author(s):

Predrag Stojanovic ◽

Nikola Stojanovic ◽

Zorica Stojanovic-Radic ◽

Valentina Arsić Arsenijević ◽

Suzana Otasevic ◽

...

Keyword(s):

Tertiary Care Hospital ◽

Antifungal Susceptibility ◽

Tertiary Care ◽

Bloodstream Infections ◽

Drug Susceptibility ◽

Antifungal Drugs ◽

Care Hospital ◽

Candida Spp ◽

Multivariate Statistical ◽

Medical Histories

Introduction: Candida spp. frequently cause hospital-acquired bloodstream infections (BSI) with a high mortality rate (up to 70%). We analyzed the frequency, infection characteristics, potential predisposing factors, susceptibility to antifungal drugs, biofilm production and other virulence characteristics of Candida spp. isolates obtained from a tertiary care hospital in Niš, Serbia, during a one year period. Methods: Medical histories, characteristics of isolated strains and drug susceptibility, as well as the effect on the function of isolated macrophages and other virulence features were evaluated. The obtained results were subjected to student’s t-test and multivariate statistical analyzes. Results: Herein we report an annual incidence of 3.65 cases of C. albicans, C. lusitaniae and C. lipolytica infections per 105 population. Out of eight isolated strains, two (25%) were shown to be strong biofilm producers, one (12.5%) caused hemolysis on blood agar and in two (25%) cases macrophages were able to completely eliminate the yeast colonies. Chronic kidney disease, diabetes, malignant and other diseases were present in 37.5, 62.5, 50 and 75%, respectively, in the study group. All patients with Candida BSI received antifungal therapy (amphotericin B), however, hospital mortality was observed in 25% of patients. Conclusions: Evaluation of local Candida epidemiology, antifungal susceptibility and virulence factors, as well as personalized patient risk factors are important for the surveillance of Candida BSI, especially in intensive care unit patients and may contribute to the improved options and outcome for patients with Candida BSI.

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